• 제목/요약/키워드: new test method

검색결과 3,990건 처리시간 0.041초

Modified sorbitol MacConkey agar for the rapid isolation of Escherichia coli O157:H7

  • Jung, Byeong-yeal;Jung, Suk-chan;Lee, Na-kyung;Cho, Seong-kun;Cho, Dong-hee;Her, Moon;Yoon, Yong-dhuk;Kim, Bong-hwan
    • 대한수의학회지
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    • 제39권4호
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    • pp.765-771
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    • 1999
  • Unlike most Escherichia coli strains, E coli O157 : H7 didn't ferment sorbitol within 24h of incubation and showed a negative reaction for $\beta$-glucuronidase. We developed a new medium for the rapid isolation of E coli O157 : H7 using sorbitol MacConkey agar with cefixime, potassium tellurite and 4-methylumbelliferyl-${\beta}$-D-glucuronide (MUG) as a primary plating medium. The addition of $20{\mu}g/ml$ of vancomycin in enrichment broth for E coli O157 : H7 inhibited lots of Gram positive bacteria. Three strains (10.3%) of 29 non-O157 E coli strains and 3 strains (8.3%) of 36 Salmonella spp were inhibited at the $0.05{\mu}g/ml$ of cefixime and 23 strains (79.3%) of 29 non-O157 E coli strains and 12 strains (33.3%) of 36 Salmonella spp were inhibited at the $2.0{\mu}g/ml$ of potassium tellurite. But none of the E coli O157 : H7 was affected at these concentration. The addition of MUG at $100{\mu}g/ml$ level to sorbitol MacConkey agar with cefixime and potassium tellurite (CTM-SMAC) aided in the rapid isolation of E coli O157 : H7 from samples by checking sorbitol-negative and $\beta$-glucuronidase negative phenotypes simultaneously. In conclusion, inoculation of a positive in the O157 screening test from enrichment broth on CTM-SMAC appeared to be a rapid, cost-effective and sensitive method for the isolation of E coli O157 : H7.

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보일러 管材料의 크리프破斷特性에 미치는 고온부식의 影響 (The Effects of Hot Corrosion on the Creep Rupture Properties of Boiler Tube Material)

  • 오세욱;박인석;강상훈
    • 대한기계학회논문집
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    • 제13권2호
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    • pp.236-242
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    • 1989
  • 본 연구에서는 주로 보일러의 과열기 및 재열기등의 관재료로 널리 사용되고 있는 304 스테인레스강에 대하여 실제 고온부식현상을 재현한 부식환경하인 630.deg. C, 690.deg. C 및 750.deg. C 3가지 온도범위에서 크리프시험을 실시하고 고온부식이 크리프파단 특성에 미치는 영향을 부식조건이 없는 고온대기중의 실험결과와 비교 검토하였다.

2002년 경주시에서 유행한 급성출혈성결막염에 관한 역학조사 (Epidemiologic Investigation into the Outbreak of Acute Hemorrhagic Conjunctivitis in Gyeongju-City, South Korea, in 2002)

  • 김홍환;민영선;임현술
    • Journal of Preventive Medicine and Public Health
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    • 제37권4호
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    • pp.312-320
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    • 2004
  • Objectives : An outbreak of acute hemorrhagic conjunctivitis (AHC) caused by coxsackie A24 (CA24) virus occurred in South Korea in 2002. CA24 was isolated for the first time from patients with AHC. Therefore, this study was conducted to understand the transmission routes and prevent another AHC outbreaks. Methods : The authors conducted a questionnaire survey among 1,730 students from 2 middle schools and 1 technical high school in Gyeongju city. For statistical analysis the chi-square test was used, and chi-square for trend method showing a level of significance less than p<0.05 was proven to be significant. Variables which were proven to be significant in univariate analysis were analysed by multiple logistic regression. Results : The attack rate was 57.1%. The student groups with rubbing one s own eyes, computer usage, and sharing cellular phone had a significantly higher AHC attack rate (p<0.05). According to the multiple logistic regression, the odds ratios for male, high school, computer use, sharing cellular phone, and rubbing one s own eyes were significant (p<0.05). Conclusion : The most significant feature of this outbreak was that many students rubbed their own eyes following contact with AHC patients in a deliberate attempt to avoid going to school. Other transmission methods were computer usage and sharing cellular phone. In the future, health and school authorities must plan new strategies for the prevention of AHC.

액정의 상전이 측정에 대한 초음파 spectroscopy 시스템의 적용 (The Application of Ultrasonic Spectroscopy System for Phase Transition of Liquid Crystal)

  • 김정구;임청환
    • 대한방사선기술학회지:방사선기술과학
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    • 제27권4호
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    • pp.31-35
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    • 2004
  • PVDF[poly(vinylidene fluoride)] 압전소자로 제작된 초음파 변환기를 이용하여 초음파 spectroscopy 측정시스템을 제작하였다. 이 장치를 이용하여 MBBA(p-methoxy benzylidene-p-n-butylaniline) 액정의 nematic-isotropic 상전이 현상을 관측한 결과 상전이 온도가 $47^{\circ}C$임을 확인하였으며, 기존의 광학적 방법에 의해 관측된 값과 잘 일치하였다. 한편으로 상전이 점의 주파수 의존성을 확인하였으나 이는 관측되지 않았으며, 단지 PVDF 압전소자의 공진주파수인 2 MHz에서 초음파의 진폭이 최대로 나타났으며, 다른 주파수에서는 진폭이 감소함을 알 수 있었다. 이와 같이 온도의 변화에 따른 액정의 상전이 현상을 초음파 감쇠의 변화로 확인하여 보았다. 이것은 액정의 상전이 메카니즘을 해석함에 있어 PVDF 초음파 변환기를 이용한 spectroscopy 측정 시스템은 매우 유용한 도구로 이용될 수 있음을 확인하였으며, 의료용으로 이용할 경우 겸상세포 빈혈증이나 동맥 경화증과 같은 질병 진단에 이용 가능하리라 여겨진다.

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정적변위를 이용한 교량의 손상도 평가기법 (A Damage Assessment Technique for Bridges Using Static Displacements)

  • 최일윤;조효남
    • 한국강구조학회 논문집
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    • 제14권5호통권60호
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    • pp.641-646
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    • 2002
  • 본 논문에서는 정적 처짐데이터를 이용한 교량의 강성추정에 관한 기법을 개발하였다. 제안된 기법은 주기적으로 교량의 자중에 의한 처짐을 계측하여 이들 처짐값의 변화량을 이용하여 손상의 진행정도를 규명하는 기법으로써 재하시험이 필요하지 않으며, 최근 활발히 진행되고 있는 계측모니터링 시스템으로부터 획득된 계측데이터를 정량적으로 분석하는 기법으로 활용이 가능하리라 판단된다. 손상평가를 위한 정식화과정에서 부재의 손상은 강성의 저감으로 표현하였으며, 부재의 질량과 초기강성은 실측 또는 도면을 통하여 획득이 가능하다고 가정하였다. 제안된 기법의 타당성 검증을 위하여 수치모형을 통한 손상도 추정결과를 제시하였으며, 기존의 손상도 추정기법 중 비교적 손상에 대한 민감도가 높은 모드형상을 이용한 기법과 손상도 추정결과를 비교하였다. 또한, 손상추정결과를 정확도를 감소시키는 노이즈의 영향을 분석하기 위하여 정적응답자료에 백색잡음을 추가하여 손상도를 추정하였으며, 손상부재의 수가 손상추정 결과에 미치는 영향을 검토하였다.

Moisture Absorption and Desorption Properties of Douglas Fir, Hinoki, Larch, Plywood, and WML Board in Response to Humidity Variation

  • PARK, Hee-Jun;JO, Seok-Un
    • Journal of the Korean Wood Science and Technology
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    • 제48권4호
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    • pp.488-502
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    • 2020
  • In this study, the moisture absorption and desorption properties presented by the Health-Friendly Housing Construction Standards of South Korea were compared using the wood of three tree species (Douglas-fir, Hinoki, Larch) and two types of wood-based materials(Plywood, WML Board). The national standards for functional building materials present that the amounts of moisture absorption and desorption should be at least 65g/㎡ on average, respectively according to the test method under KS F 2611:2009. Therefore, in this study, the moisture absorption/desorption properties of materials with no treatment (Control), with punching, and with surface stain finishing and the moisture absorption/desorption property improvement effects of the treatments were compared and analyzed. According to the results of this study, it was evaluated that all five types of wood and wood-based materials tested did not satisfy the amount of moisture absorption/desorption of at least 65g/㎡, which is the performance standard for moisture absorption/desorption functional building materials, indicating that untreated wood and wood-based materials cannot be applied as functional finishing materials according to the Health-Friendly Housing Construction Standards. The surface stain finishing greatly reduced the moisture absorption and desorption rates of the materials, and the amounts of moisture absorbed and desorbed were also shown to decrease by at least two times on average. When the surfaces of the materials were punched with Ø4mm holes at intervals of 20 mm, the moisture absorption/desorption areas increased from 18% to 51%, and this increase was shown to be capable of increasing the amounts of moisture absorbed/desorbed by 29% on average at the minimum, and 81% on average at the maximum. The effects of punching were shown to be identical even in cases where the materials were stain finished. For the application of wood or wood-based materials as eco-friendly, health-friendly, and moisture absorption/desorption functional building materials hereafter, it is judged that new physical and chemical improvement studies should be conducted, and treatment methods should be developed.

트랙터 부착형 자동 토양경도 측정 시스템 개발 (Development of an Automatic Soil Hardness Measuring System Mountable on Agricultural Tractors)

  • 이현동;김기대;김찬수;김성환
    • Journal of Biosystems Engineering
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    • 제27권6호
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    • pp.537-546
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    • 2002
  • In this study an automatic soil hardness measuring system mountable on agricultural tractors was developed to improve the accuracy of manual soil hardness testers by a constant penetrating rate, right direction of the cone-penetrometer and the isolation of vibration from the operator. This was necessary to supply similar experimental condition for performance test of new model and comparative experiment. The results of the study are summaried as follows; 1. The system consisted of a sensing part of soil hardness, a driving part of the measuring system and an attaching part between the tractor and the measuring system. 2. The allowable limit value of the system developed was set to 392N to protect from breaking the serve motor and the coupling used in this system. 3. The driving shaft penetrated into soil by 0.3m to measure soil hardness. The soil hardness was measured at the depth of 0.3m from the soil surface but the penetrating work was stopped and the driving shaft was pulled out to protect the system when the value of the soil hardness was too big on foreign substances like stones or straws. 4. Two values measured by automatic measuring system developed in this research and manual penetrometer were compared by statistics hypothesis testing method. When two people measured the soil hardness at the depth of 0.1 and 0.15m by manual cone penetrometer, there was no relationship between two values by two people but the values at the same depths by automatic measuring system developed showed similarity. The automatic system, therefore, developed in this research was proper for measuring soil hardness.

Development of a Prototype of Guidance System for Rice-transplanter

  • Zhang, Fang-Ming;Shin, Beom-Soo;Feng, Xi-Ming;Li, Yuan;Shou, Ru-Jiang
    • Journal of Biosystems Engineering
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    • 제38권4호
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    • pp.255-263
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    • 2013
  • Purpose: It is not easy to drive a rice-transplanter avoiding underlapped or overlapped transplanting in paddy fields. An automated guidance system for the riding-type rice-transplanter would be necessary to operate the rice-transplanter autonomously or to assist the beginning drivers as a driving aid. Methods: A prototype of guidance system was composed of embedded computers, RTK-GPS, and a power-steering mechanism. Two Kalman filters were adopted to overcome sparse positioning data (1 Hz) from the RTK-GPS. A global Kalman filter estimated the posture of rice-transplanter every one second, and a local Kalman filter calculated the posture from every new estimation of the global Kalman filter with an interval of 200 ms. A PID controller was applied to the row-following mode control. A control method of U-turning mode was developed as well. A stepping motor with a reduction gear set was used to rotate the shaft of steering wheel. Results: Test trials for U-turning and row-following modes were done in a paddy field after some parameters have been tuned at the ground speed range of 0.3 ~ 1.2 m/s. The minimum RMS error of offset was 3.13 cm at the ground speed of 0.3 m/s while the maximum RMS error was 13.01 cm at 1.2 m/s. The offset RMS error tended to increase as the ground speed increased. The target point distance, LT also affected the system performance and PID controller parameters should be adjusted on different ground speeds. Conclusions: A target angle-based PID controller plus stationary steering angle controller made it possible for the rice-transplanter to steer autonomously by following a reference line accurately and even on U-turning mode. However, as condition in paddy fields is very complicated, the system should control the ground speed that prevents it from deviating too much due to ditch and slope.

Insights into the in vitro germicidal activities of Acalypha indica

  • Rahman, Md. Shahedur;Hossain, Riad;Saikot, Forhad Karim;Rahman, Shaikh Mizanur;Saha, Subbroto Kumar;Hong, Jongki;Kim, Ki-Hyun
    • 분석과학
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    • 제30권1호
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    • pp.26-31
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    • 2017
  • Background and purpose: This study was carried out to learn more about the potential prophylactic or antibacterial activity of the plant Acalypha indica against selective pathogenic bacteria. Experimental: The test organisms were Sarcina lutea IFO 3232, Bacillus subtilis IFO 3026, Pseudomonas denitrificans, Escherichia coli IFO 3007, Klebsiella pneumoniae ATTC 10031, Xanthomonas campestris IAM 1671, and Proteus vulgaris. Leaf, stem, and bud powder of Acalypha indica were dissolved in various solvents, and the extracts were tested for antimicrobial activity through the disc diffusion method. GC-MS profiling was performed to characterize active chemical compounds in the essential oil of Acalypha indica. Results: The ethanol extract showed the highest activity against all bacteria, while the petroleum ether extract yielded the highest zone of inhibition against Proteus vulgaris ($11.83{\pm}1.75mm$). The minimum inhibitory concentration (MIC) of the ethyl acetate extract against Bacillus subtilis was 16 µg/mL. Phytochemical screening by GC-MS revealed a total of 12 bioactive compounds. Conclusion: Extracts of Acalypha indica may be useful in formulating and synthesizing new antibacterial drugs.

실험비용을 고려한 적응적 실험설계법 기반 KF-16 피칭모멘트계수 모델링 (Pitching Moment Coefficient Modeling of KF-16 using Adaptive Design of Experiments with cost consideration)

  • 이돈구;진현;안재명;이영빈
    • 한국항공우주학회지
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    • 제44권6호
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    • pp.537-543
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    • 2016
  • KF-16의 1/33 축소 모형 공력 데이터베이스를 기반으로 풍동실험에 적응적 실험설계법을 적용하는 경우 실험의 정확도뿐만 아니라 풍동 실험에 소요되는 비용을 최소화할 수 있도록 추가 실험점 선택 방법에 대한 연구를 수행하였다. 라틴방격법을 이용하여 초기실험점을 선택하였고, Gaussian Process를 통해 불확실성이 가장 크면서도 실험에 소요되는 비용이 상대적으로 적은 추가 실험점을 선택하였다. 공력계수 모델링은 가장 비선형성이 큰 피칭모멘트계수를 정확히 모델링 하는 것을 목표로 하였다. 실험 비용을 고려한 적응적 실험설계법을 적용한 경우, 기존의 적응적 실험설계 방법론에 비해 모델의 정확도와 실험에 소요되는 비용에 어떤 영향을 미치는지 알아보았다.