• Korean Chemical Engineering Research
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• v.60 no.3
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• pp.398-406
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• 2022

The outer membrane of Gram-negative bacteria is the outermost layer of cellular environment in which numerous biophysical and biochemical processes are in action sustaining viability. Advances in cell engineering enable modification of bacterial genetic information that subsequently alters membrane physiology to adapt bacteria to specific purposes. Surface display of a functional molecule on the outer membranes is one of strategies that directs host cells to respond to a specific extracellular matter or stimulus. While intracellular expression of a functional peptide or protein fused to a membrane-anchoring motif is commonly practiced for surface display, the method is not readily applicable to exogenous or large proteins inexpressible in bacteria. Chemical conjugation at reactive groups naturally occurring on the membrane might be an alternative, but often compromises fitness due to non-specific modification of essential components. Herein, we demonstrated two distinct approaches that enable site-specific decoration of the outer membrane with a fluorescent agent in Escherichia coli. An unnatural amino acid genetically incorporated in a surface-exposed peptide could act as a chemoselective handle for bioorthogonal dye labeling. A surface-displayed α-helical domain originating from a part of a selected heterodimeric coiled-coil complex could recruit and anchor a green fluorescent protein tagged with a complementary α-helical domain to the membrane surface in a site- and hetero-specific manner. These methods hold a promise as on-demand tools to confer new functionalities on the bacterial membranes.

• Journal of the Korean Electrochemical Society
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• v.25 no.1
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• pp.1-12
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• 2022

Lithium metal is the most promising anode for next-generation lithium-ion batteries due to its lowest reduction potential (-3.04 V vs. SHE) and high specific capacity (3860 mAh/g). However, the dendritic formation under high charging current density remains one of main technical barriers to be used for commercial rechargeable batteries. To address these issues, tremendous research to suppress lithium dendrite formation have been conducted through new electrolyte formulation, robust protection layer, shape-controlled lithium metal, separator modification, etc. However, Li/Li symmetric cell test is always a starting or essential step to demonstrate better lithium dendrite formation behavior with lower overpotential and longer cycle life without careful analysis. Thus, this review summarizes overpotential behaviors of Li/Li symmetric cells along with theoretical explanations like initial peaking or later arcing. Also, we categorize various overpotential data depending on research approaches and discuss them based on peaking and arcing behaviors. Thus, this review will be very helpful for researchers in lithium metal to analyze their overpotential behaviors.

• Journal of Internet Computing and Services
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• v.24 no.1
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• pp.71-86
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• 2023

The purpose of this exploratory study is to compare the differences in public perceptual characteristics of Korean and Chinese societies regarding the metaverse using big data analysis. Due to the environmental impact of the COVID-19 pandemic, technological progress, and the expansion of new consumer bases such as generation Z and Alpha, the world's interest in the metaverse is drawing attention, and related academic studies have been also in full swing from 2021. In particular, Korea and China have emerged as major leading countries in the metaverse industry. It is a timely research question to discover the difference in social awareness using big data accumulated in both countries at a time when the amount of mentions on the metaverse has skyrocketed. The analysis technique identifies the importance of key words by analyzing word frequency, N-gram, and TF-IDF of clean data through text mining analysis, and analyzes the density and centrality of semantic networks to determine the strength of connection between words and their semantic relevance. Python 3.9 Anaconda data science platform 3 and Textom 6 versions were used, and UCINET 6.759 analysis and visualization were performed for semantic network analysis and structural CONCOR analysis. As a result, four blocks, each of which are similar word groups, were driven. These blocks represent different perspectives that reflect the types of social perceptions of the metaverse in both countries. Studies on the metaverse are increasing, but studies on comparative research approaches between countries from a cross-cultural aspect have not yet been conducted. At this point, as a preceding study, this study will be able to provide theoretical grounds and meaningful insights to future studies.

• Economic and Environmental Geology
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• v.56 no.4
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• pp.475-499
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• 2023

To infer the provenance of raw iron materials utilized in iron production at the archaeological sites in Gyeongsang province, petrographic and geochemical analyses were conducted for smelting samples and major iron ores sourced from ore deposits. The smelting samples excavated from various iron archaeological sites were classified into different types according to their refining processes, such as iron bloom, iron bloom slag, pig iron, pig iron slag, forging iron flake, smithery iron, iron flake, and arrowhead. These samples exhibited discernable differences in their mineralogical components and texture. The enrichments of major elements such as aluminum and calcium in silicate minerals of the residual slags and the high contents of trace elements such as nickel and copper in some iron-making relics reflect the characteristics of raw iron ores, and thus can be regarded as potential indicators for inferring the provenance of source materials. In particular, the compositional ranges of Pb-Sr isotope ratios for the iron smelting samples were classified into three categories: 1) those exhibiting similar ratios to those of the raw iron ores, 2) those enriched in strontium isotope ratio, and 3) those enriched in both lead and strontium isotope ratios. The observed distinct Pb-Sr isotope characteristics in the iron smelting samples suggest the potential contribution of specific additives being introduced during the high-temperature refining process. These results provide a new perspective on the interpretation of the provenance study of the iron archaeological samples in Gyeongsang province, particularly in terms of the potential contribution of additives on the refining process.

• Journal of Science Education
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• v.47 no.2
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• pp.117-126
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• 2023

In an elementary school science teacher's guide, the guide in details that can help elementary school teachers with difficulties when preparing and conducting science classes are essential. To gain insights into the development of the 2022 revised curriculum elementary school science teacher's guide, it is necessary to investigate the perceptions and needs of elementary school teacher's guide in details. In this study, we developed a questionnaire by analyzing the components and design of an elementary school science teacher's guide in details to explore elementary school teachers' perceptions and needs. For this purpose, we first investigated elementary school teachers' perceptions of their needs, satisfaction, and the utilization of each component of the current guide in details. Next, we investigated teachers' needs regarding the specific components and design of a guide. The findings were as follows. First, elementary school teachers were delighted with the components that help them prepare and conduct lessons. Second, elementary school teachers wanted an easy-to-read design with a layout that allowed them to see the components they needed for their lessons at a glance. In conclusion, the elementary school science teacher's guide in details to be readable and organized to provide at-a-glance information on lesson preparation, lesson flow, and teaching and learning materials needed for science teaching-learning. Based on the results of this study, new approaches and attempts should be made to develop a textbook that elementary school teachers can utilize in the future.

• Journal of the Computational Structural Engineering Institute of Korea
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• v.36 no.1
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• pp.9-18
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• 2023

Predicting the compressive strength of high-performance concrete (HPC) is challenging because of the use of additional cementitious materials; thus, the development of improved predictive models is essential. The purpose of this study was to develop an HPC compressive-strength prediction model using an ensemble machine-learning method of combined bagging and stacking techniques. The result is a new ensemble technique that integrates the existing ensemble methods of bagging and stacking to solve the problems of a single machine-learning model and improve the prediction performance of the model. The nonlinear regression, support vector machine, artificial neural network, and Gaussian process regression approaches were used as single machine-learning methods and bagging and stacking techniques as ensemble machine-learning methods. As a result, the model of the proposed method showed improved accuracy results compared with single machine-learning models, an individual bagging technique model, and a stacking technique model. This was confirmed through a comparison of four representative performance indicators, verifying the effectiveness of the method.

• Journal of the Korean Geotechnical Society
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• v.25 no.10
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• pp.87-97
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• 2009

The seismic site coefficients are often used with the seismic hazard maps to develop the design response spectrum at the surface. The site coefficients are most commonly developed deterministically, while the seismic hazarde maps are derived probabilistically. There is, hence, an inherent incompatibility between the two approaches. However, they are used together in the seismic design codes without a clear rational basis. To resolve the fundamental imcompatibility between the site coefficients and hazard maps, this study uses a novel probabilistic seismic hazard analysis (PSHA) technique that simulates the results of a standard PSHA at a rock outcrop, but integrates the site response analysis function to capture the site amplification effects within the PSHA platform. Another important advantage of the method is its ability to model the uncertainty, variability, and randomness of the soil properties. The new PSHA was used to develop fully probabilistic site coefficients for site classes of the seismic design code and another sets of site classes proposed in Korea. Comparisons highlight the pronounced discrepancy between the site coefficients of the seismic design code and the proposed coefficients, while another set of site coefficients show differences only at selected site classes.

• Journal of Daesoon Thought and the Religions of East Asia
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• v.2 no.2
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• pp.33-50
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• 2023

'Fasting of the Mind (心齋, ch. xīn zhāi)' is arguably the most important concept within the practical approach to the spiritual cultivation formulated by the Daoist philosopher, Zhuangzi (莊子). Most scholars have interpreted 'Fasting of the Mind' as an apophatic practice centered around the aim of the 'Dissolution of the Self (喪我, ch. sàng wŎ).' The Korean new religious movement, Daesoon Jinrihoe (大巡眞理會), can be shown to instead consistently utilize cataphatic descriptions of spiritual cultivation based on the 'quieting of the mind (安心, kr. anshim)' and 'quieting of the body (安身, kr. anshin)' with the highest attainable state referred to as the 'Perfected State of Unification with the Dao (道通眞境, kr. Dotong-jingyeong).' While the language used by Zhuangzi and Daesoon Jinrihoe appears quite different on a superficial level, a deeper examination shows that these rhetorical framings are likely negativistic and positivistic descriptions of the same, or at least reasonably similar, phenomena. Zhuangzi, who focused primarily on the body, mind, and internal energy, cautioned practitioners that 'mere listening stops with the ears (聽止於耳, ch. tīng zhǐ yú ěr)' and 'mere recognition stops with the mind (心止於符, ch. xīn zhǐ yú fú).' He therefore encouraged cultivators of the Dao to 'listen with the spirit (聽之以氣 ch. tīng zhī yǐ qì).' The main scripture of Daesoon Jinrihoe states that "The mind is a pivot, gate, and gateway for gods; They, who turn the pivot, open, and close the gate, and go back and forth through the gateway, can be either good or evil (心也者, 鬼神之樞機也, 門戶也,道路也)," and the Supreme God of the Ninth Heaven (九天上帝, kr. Gucheon Sangje) even promises to visit anyone who possesses a 'singularly-focused mind (一心, kr. il-shim).' In both these approaches, there is a sense of what must be kept out of the mind (e.g., external disturbances, strong emotions, malevolent entities) and what the mind should connect with to attain spiritual progress (e.g., spirit, singular focus, the Supreme God). The observations above serve as the main basis for a comparison between the apophatic descriptions of cultivation found in Zhuangzi and their cataphatic counterparts in Daesoon Thought. However, the culmination of this nuanced comparative exploration reveals that while the leanings of Zhuangzi and Daesoon Thought generally hold true, ultimately, both systems of cultivation transcend the categories of apophatic and cataphatic.

• Asia-pacific Journal of Multimedia Services Convergent with Art, Humanities, and Sociology
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• v.7 no.6
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• pp.897-906
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• 2017

Projection Mapping, also known as Spatial Augmented Reality(SAR) has attracted much attention recently and used for many division, which can augment physical objects with projected various virtual replications. However, conventional approaches towards projection mapping have faced some limitations. Target objects' geometric transformation property does not considered, and movements of flexible objects-like paper are hard to handle, such as folding and bending as natural interaction. Also, precise registration and tracking has been a cumbersome process in the past. While there have been many researches on Projection Mapping on static objects, dynamic projection mapping that can keep tracking of a moving flexible target and aligning the projection at interactive level is still a challenge. Therefore, this paper propose a new method using Unity3D and ARToolkit for high-speed robust tracking and dynamic projection mapping onto non-rigid deforming objects rapidly and interactively. The method consists of four stages, forming cubic bezier surface, process of rendering transformation values, multiple marker recognition and tracking, and webcam real time-lapse imaging. Users can fold, curve, bend and twist to make interaction. This method can achieve three high-quality results. First, the system can detect the strong deformation of objects. Second, it reduces the occlusion error which reduces the misalignment between the target object and the projected video. Lastly, the accuracy and the robustness of this method can make result values to be projected exactly onto the target object in real-time with high-speed and precise transformation tracking.

• The Journal of Bigdata
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• v.6 no.2
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• pp.17-28
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• 2021

Current development of technologies related to 4^th industrial revolution and the pandemic of COVID-19 lead the rapid expansion of e-marketplace. The level of competition among several companies gets increased by introducing different strategies. To cope with the current change in the market and satisfy the customers who request the better delivery service, the new concept, fulfillment, has been introduced. It makes the leadtime of process from order picking to delivery reduced and the efficiency improved. Still, the efficiency of operation in fulfillment centers constrains the service level of the entire delivery process. In order to solve this problem, several different approaches for demand forecasting and coordinating supplies using Bigdata, IoT and AI, which there exists the trivial limitations. Because it requires the most lead time for operation and leads the inefficiency the process from picking to packing the ordered items, the logistics service providers should try to automate this procedure. In this research, it has been proposed to develop the efficient plans to automate the process to move the ordered items from the location where it stores to stage for packing using AGV and AMR. The efficiency of automated devices depends on the number of items and total number of devices based on the demand. Therefore, the result of simulation based on several different scenarios has been analyzed. From the result of simulation, it is possible to identify the several factors which should be concerned for introducing the automated devices in the fulfillment centers. Also, it can be referred to make the optimal decisions based on the efficiency metrics.