• Title/Summary/Keyword: neutral sugar

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Studies on the Induction of Available Mutants of Takju Yeast by UV light Irradiation (part 2) -On the Physiological Characteristics of the Mutants- (자외선조사(紫外線照射)에 의한 탁주효모(酵母)의 변이주육성(變異株育成)에 관한 연구 (제 2 보) -변이주(變異株)의 생리적성질(生理的性質)에 관하여)

  • Kim, Chan-Jo;Oh, Man-Jin;Kim, Seung-Yul
    • Applied Biological Chemistry
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    • v.18 no.1
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    • pp.16-22
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    • 1975
  • This experiment was carried out to investigate the physiological characteristics of two original yeasts, 5-Y-5 and 6-Y-6, which selected from 24 Takju yeasts and three mutants, 30-24,30-81 and 40-27. induced from two original yeasts by the irradiation of UV light. The results were summarized as follows. 1) Alcohol tolerances of three mutants were decreased in some degree as compared with those of original yeasts. 2) Tolerances of lactic and citric acids of acid producing mutant 30-81, was increased than those of original yeasts. 3) In the case of using ammonium sulfate as a nitrogen source, two original yeasts and three mutants required Ca-pantothenate as a essential growth factor and four strains of yeasts except the mutant, 30-81, required biotin as a stimulated growth factor, When asparagine was used as a nitrogen source, two original yeasts and three mutants showed the same as above result but the stimulated effect of biotin was far less. 4) Propagation powers of the mutants were weaken than those of original yeasts, particular that of acid producing mutant, 30-81, was the weakest in the three mutants. 5) The optimum temperature for fermentation of original yeasts were $30^{\circ}C\;to\;35^{\circ}C$ but three mutants were $25^{\circ}C\;to\;30^{\circ}C$. 6) The optimum pH for fermentation of original yeasts were pH 5 to 6, and there is no appreciable difference between original yeasts and three mutants. The fermentation power of mutant,30-81, was decreased more rapidly than those of other mutants according to approach neutral. Three mutants were more sensible to heat than original yeasts. 7) Two original yeasts and three mutants were inhibited more over 20 percent of sugar for fermentation and three mutants were more sensible to sugar concentration than original yeasts.

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Studies on the Effect of Garlic on the Enzyme Production and Growth of Aspergillus oryzae (국균(麴菌)의 생육(生育) 및 효소생산(酵素生産)에 미치는 마늘성분(成分)에 관한 연구(硏究))

  • Lee, Suk-Kun;Lee, Taik-Soo;Nam, Sung-Hee
    • Applied Biological Chemistry
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    • v.21 no.2
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    • pp.123-130
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    • 1978
  • Asp. oryzae D and H strains were cultured in the wheat bran and Czapek-Dox liquid media containing garlic powder in order to investigate the effect of garlic on the enzyme production and growth of Asp. oryzae. The results obtained were as follows; 1. Wheat bran media containing 0.5 to 2% garlic powder yielded increased in acid protease activity of the Asp. oryzae D strain while the best activity appeared at 2 to 6% in alkaline protease and 0.5% in neutral protease. 2. The protease activities of Asp. oryzae H strain was similar to that of the control in wheat bran media containing 0.5 to 8% garlic powder, but the peak appeared at the garlic powder concentration of 30%. 3. Garlic powder increased the $\alpha$-and Glucoamylase activities of Asp. oryzae H strain. 4. Garlic powder inhibited the both Asp. oryzae strains from the cellulase production. 5. Czapek-Dex liquid media containing 0.5 to 6% garlic powder yielded increase in dry mycerial weight in comparison with the control, and the increament was much more in case of the Asp. oryzae H strain. 6. As the amount of the garlic powder added to the Czapek-Dox liquid media increased, the pH of the cultured media of Asp. oryzae D strains was on the decrease while the media of H strain increased. 7. As the amount of the garlic powder added increased, the acidity of the cultured Czapek-Dex media increased. 8. The growth rate of the two Asp. oryzae strains were remarkably inhibited and no growth appeared in wheat bran and Czapek-Dox media containing garlic powder more over 10%. 9. The utility rate of reducing sugar was the highest in the Czapek-Dox liquid media containing 1 to 2% garlic powder.

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Physicochemical Stability of Anthocyanins from a Korean Pigmented Rice Variety as Natural Food Colorants (천연색소로서 한국산 유색미 안토시아닌의 안정성 연구)

  • Yoon, Joo-Mi;Cho, Man-Ho;Hahn, Tae-Ryong;Paik, Young-Sook;Yoon, Hye-Hyun
    • Korean Journal of Food Science and Technology
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    • v.29 no.2
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    • pp.211-217
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    • 1997
  • The physical and chemical stability of anthocyanins from a Korean pigmented rice variety was investigated at various conditions of pH, temperature, metal ion, sugar, organic acid and light. The anthocyanin pigments were relatively stable with half-lives of 36 days (pH 2.0) and 17 days (pH 3.0), while they were decomposed in a day at neutral and basic pH of 7.0 and 9.0 at $25^{\circ}C$. The anthocyanins also showed high thermal stability at pH 3.0; the half-lives were 7.4 hr, 23.6 hr and 96.3 hr at $95^{\circ}C,\;75^{\circ}C\;and\;50^{\circ}$, respectively. Addition of di- and tri-valent metal ions at pH 3.0 resulted in the increase of color intensity and stability throughout 21 days of storage periods at $25^{\circ}C$. Most sugars added accelerated the degradation of anthocyanin pigments, so that fructose showed the greatest degradation effect on the pigments. Addition of citric acid at pH 3.0 increased stability of anthocyanins, while tartaric acid decreased stability. The anthocyanins were very sensitive on light irradiation with a degradation half-life of 14 hr under 20,000 lux-light dosage at pH 3.0.

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Effect of Application Level of Swine Slurry on Growth Characteristics and Yield of Sorghum$\times$Sudangrass Hybrid and $NO_3-N$ Content in Infiltration Water (돈분액비 시용수준이 수수$\times$수단그라스 교잡종의 생육특성, 수량 및 용탈수 중 $NO_3-N$ 함량에 미치는 영향)

  • Lim Young-Chul;Yoon S.H.;Kim J.G.;Kim W.H.;Choi G.J.;Seo S.;Yook W.B.
    • Journal of Animal Environmental Science
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    • v.12 no.1
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    • pp.35-40
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    • 2006
  • This experiment was conducted to investigate the effect of application level of swine slurry on the growth characteristics and yield of sorghum$\times$sudangrass hybrid and $NO_3-N$ content in infiltration at experimental field of Grassland and Forage Crops Division, National Livestock Research Institute, RDA from 2000 to 2002. Treatments were consisted of non fertilizer(NF), chemical fertilizer(CF), 100% swine slurry(SS 100), 150% swine slurry(SS 150), 200% swine slurry(SS 200) and 100% swine slurry + CF 50%(SS100 + CF 50) with randomized complete block design and three replications. Growth of sorghum$\times$sudangrass hybrid was not nearly different among the treatments, but early growth of swine slurry treatments was better than that of CF, and regrowth after 1st cutting was shown better in CF and SS 100+CF 50 with adding application of chemical fertilizer. The sugar content(brix %) was tends to be increased with swine slurry application. Dry matter(DM) yields of SS 100 and SS 150 were lower 15 and 6% than that of CF, respectively, and SS 200 was similer to CF, but there was not found significant difference among all treatments. The content of crude protein(CP), acid detergent fiber(ADF), and neutral detergent fiber(NDF) did not show the difference. The content of $NO_3-N$ in infiltration water was not more than CF by the 55 150 application, but more than by SS 200 and 55 100+CF 50 treatment.

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Comparison of Agronomic Characteristics, Forage Production and Quality of Kenaf (Hongma 300), Maize (Kwangpyeongok) and Sorghum × Sudangrass Hybrids (Jumbo) in Middle Region of Korea (중부지역에서 케나프(홍마 300), 옥수수(광평옥) 및 수수×수단그라스 교잡종(점보)의 생육특성, 수량성 및 품질비교)

  • Hwang, Tae-Young;Ji, Hee Chung;Kim, Ki Yong;Lee, Sang-Hoon;Lee, Ki-Won;Choi, Gi Jun
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.35 no.2
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    • pp.152-158
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    • 2015
  • This study was conducted from 2013 to 2014 in the middle region of Korea to compare the agronomic characteristics, forage production, and quality of kenaf, maize, and a sorghum ${\times}$ sudangrass hybrid. The three crops used in this study were kenaf "Hongma 300," maize "Kwangpyeongok," and the sorghum ${\times}$ sudangrass hybrid "Jumbo" (headless type). The sugar contents of the kenaf, maize, and sorghum ${\times}$ sudangrass hybrid were 5.4%, 4.4%, and 2.5%, respectively. The emergence rates and lodgings of the three crops were sound, as they were more than 80% and 1.5, respectively. The fresh yields of kenaf, maize, and the sorghum ${\times}$ sudangrass hybrid were 117,521 kg/ha, 73,336 kg/ha, and 133,334 kg/ha, respectively, and the dry matter (DM) yields were 28,598 kg/ha, 19,951 kg/ha and 20,083 kg/ha, respectively. The DM yield of kenaf was significantly the highest among the three crops (p<0.05). However, the total digestible nutrients (TDN) contents of the kenaf, corn, and sorghum ${\times}$ sudangrass hybrid were 43.3 %, 72.0 %, and 54.8 %, respectively, and the in vitro digestible dry matter (IVDDM) yields were 44.4%, 73.7%, and 59.6 %, respectively; therefore, the TDN yield of the corn was 13 % significantly higher than that of the kenaf (p<0.05). The TDN yields of the kenaf, corn, and sorghum ${\times}$ sudangrass hybrid were 12,472 kg/ha, 14,350 kg/ha, and 11,001 kg/ha, respectively. Among the three crops, the content of neutral detergent fiber (NDF) and acid detergent fiber (ADF) were highest in the kenaf. This study shows that the kenaf had a good forage productivity but a low forage quality.

Quality Characteristics of Bijijang in Different Fermentation Conditions (발효 조건을 달리한 비지장의 품질특성)

  • Im, Sung-Kyung;Yoo, Seon-Mi;Kim, Tae-Young;Chun, Hye-Kyung
    • Korean Journal of Food Science and Technology
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    • v.36 no.3
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    • pp.448-455
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    • 2004
  • Changes in quality characteristics of Bijijang (fermented soybean curd residus) prepared at $35^{\circ}C\;and\;40^{\circ}C$ for 0, 12, 24, 36, and 48 hr were investigated. Acidity of Bijijang increased, whereas pH and Hunter's color values decreased during fermentation. Immediately after Bijijang preparation, ${\alpha}-and\;{\beta}-amylase$ activities were very low, ${\beta}-Amylase$ activity during fermentation increased rapidly, with those fermented at $40^{\circ}C$ higher than at $35^{\circ}C$. Neutral pretense activity was significantly higher than acidic pretense activity, and increased gradually after 12 hr. Change in total nitrogen content in Bijijang was insignificant, whereas contents of amino-type and water-soluble nitrogens increased significantly during fermentation. Major free amino acids of Bijijang were Arg, Pro, Glu, Thr, Ser, and Lys at initial fermenting stage, and, as fermentation progressed, contents of Cys, Met Glu, Ile, Leu, and Phe increased. Reducing sugar contents of Bijijang fermented at $40^{\circ}C$ were higher than those fermented at $35^{\circ}C$. Sucrose content decreased and glucose content increased. Glucoside (genistin and daidzin) contents decreased and aglycone (genistein and daidzein) contents increased during preparation of Biji and fermentation of Bijijang. Contents of free sugars and isoflavones were higher in Bijijang fermented at $40^{\circ}C$ than at $35^{\circ}C$. Based on these results, fermentation at $40^{\circ}C$ for 48 hr was determined to be optimum fermentation condition for Bijijang.