• Neonatal Medicine
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• 제18권1호
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• pp.1-5
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• 2011

Coagulation involves the regulated sequence of proteolytic activation of a series of proteins to achieve appropriate and timely hemostasis in an injured vessel. In the non-pathological state, the inciting event involves exposure of circulating factor VIIa to extravascularly expressed tissue factor, which brings into motion the series of steps which results in cell based model of coagulation. In the new concepts of coagulation system, initiation, amplification and propagation steps are involved to converse of fibrinogen to fibrin. The precisely synchronized cascade of events is counter-balanced by a system of anticoagulant mechanisms. Developmental hemostasis refers to the age-related changes in the coagulation system that are most marked during neonate and childhood. An understanding of these changes in crucial to the accurate diagnosis of hemostatic abnormalities in neonate and children. This review aims to elucidate the main events within the coagulation cascade as it is currently understood to operate in vivo, and also a short review of the anticoagulants as they relate to this model. Also this paper describes the common pitfalls observed in the clinical data related to the coagulation system in neonate to children.

• Clinical and Experimental Pediatrics
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• 제50권3호
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• pp.223-229
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• 2007

The fetus is completely dependent on mother for glucose and other nutrient transfer across the placenta. At birth, when the maternal supply is discontinued, the neonate must adjust to an independent existence. The changes in the neonate's glucose homeostasis during this transition to the extrauterine environment are influenced by the mother's metabolism and intrinsic fetal and placental problems. Maturation of carbohydrate homeostasis results from a balance between substrate availability and coordination of developing hormonal, enzymatic, and neural systems. These mechanisms may not be fully developed in neonates, so the neonate is vulnerable to carbohydrate disequilibrium resulting in damage to the central nervous system. Hypoglycemia is a relatively common metabolic problem seen during newborn care. However its definition, management and long term sequalae remain controversial. Hyporglycemia occurs frequently as a transient disorder with excellent prognosis. It also may persist and recur and cause permanent neurological complications. Although the key to effective treatment of hypoglycemia is diagnostic specific, the maintenance of euglycemia is critical to the preservation of central nervous system function. This article discusses physiology of perinatal glucose homeostasis, focusing on evaluation and treatment of hypoglycemia.

• International Journal of Oral Biology
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• 제32권1호
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• pp.13-22
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• 2007

The stem cell research is emerging as a cutting edge topic for a new treatment for many chronic diseases. Recently, dental stem cell would be possible for regeneration of tooth itself as well as periodontal tissue. However, the study of the cell characterization is scarce. Therefore, we performed the genetic profiling and the characterization of mouse fetus/neonate derived dental tissue and cell to find the identification during dental development. We separated dental arch from mandibles of 14.5 d fetal mice and neonate 0 d under the stereoscope, and isolated dental cells primarily from the tissues. Then, we examined morphology and the gene expression profiles of the primary cells and dental tissues from fetus/neonate and adult with RT-PCR. Primary dental cells showed heterogeneous but the majority was shown as fibroblast-like morphology. The change of population doubling time levels (PDLs) showed that the primary dental cells have growth potential and could be expanded under our culture conditions without reduction of growth rate. Immunocytochemical and flow cytometric analyses were performed to characterize the primary dental cell populations from both of fetus (E14.5) and neonate. Alpha smooth muscle actin (${\alpha}-SMA$), vimentin, and von Willebrand factor showed strong expression, but desmin positive cells were not detected in the primary dental cells. Most of the markers were not uniformly expressed, but found in subsets of cells, indicating that the primary dental cell population is heterogeneous, and characteristics of the populations were changed during culture period. And mesenchymal stem cell markers were highly expressed. Gene expression profile showed Wnt family and its related signaling molecules, growth factors, transcription factors and tooth specific molecules were expressed both fetal and neonatal tissue. The tooth specific genes (enamelin, amelogenin, and DSPP) only expressed in neonate and adult stage. These expression patterns appeared same as primary fetal and neonatal cells. In this study we isolated primary cells from whole mandible of fetal and neonatal mice. And we investigated the characteristics of the primary cells and the profile of gene expressions, which are involved in epithelial-mesenchymal interactions during tooth development. Taken together, the primary dental cells in early passages or fetal and neonatal mandibles could be useful stem cell resources.

• Journal of Korean Neurosurgical Society
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• 제39권1호
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• pp.52-57
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• 2006

Objective : It has been suggested that the occurrence of persistent pain signal during the early postnatal period may alter an individual's response to pain later in life. The aim of this study is to assess whether neonatal nerve injury resulted in long-lasting consequences on nociceptive system in the rat. Methods : We examined whether neuropathic pain behaviors and the changes of spinal neuropeptides [SP, CGRP, VIP and VIP] induced by peripheral nerve injury within 1 day after birth [Neonate group] were different from those at 8 weeks after birth [Mature group]. Results : The Neonate group showed more robust and long-lasting pain behaviors than the Mature group. Immunohistochemical findings demonstrated that spinal SP- & CGRP-immunoreactivities[ir] of the ipsilateral to the contralateral side increased in the Neonate group, whereas those decreased in the Mature group. In addition, increase in spinal VIP- & NPY-ir of the ipsilateral to the contralateral side was more robust in the Mature group than in the Neonate group. Conclusion : These results suggest that peripheral nerve injury in the early postnatal period may result in long-lasting and potentially detrimental alterations in nociceptive pathways.

• 부모자녀건강학회지
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• 제3권2호
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• pp.18-24
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• 2000

The purpose of this research is to investigate Sleep/Activity pattern of normal neonate in Kangwon area. The subjects of this research were 23 normal neonate who visited pediatric clinic of kangneung general hospital from Jun 15, 1999 to August 10, 2000. The instrument of this research was the NCASA. The results of this research were as follows: 1. The mean time of daytime sleep was 8.21 hours. The mean time of nighttime sleep was 5.70 hours. The mean time of total daily sleep was 13.92 hours. The mean time of longest sleep period was 5.02 hours. The mean time of regularity of sleep were daytime 46.59%, nighttime 74.43%, total daily sleep 55.88%. The mean frequency of nighttime wakening was 2.03 times. 2. The each mean time of activity were daytime 7.72 hours, nighttime 2.28 hours, daily total activity 9.99 hours. The mean time of the longest activity period was 6.16 hours. The mean times of feeding frequency were daytime feeding 4.53, nighttime feeding 1.92, total daily feeding 6.45. The mean of regularity of feeding frequency was 65.71%.

• 여성건강간호학회지
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• 제17권4호
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• pp.426-437
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• 2011

Purpose: The purpose of this study was to identify the effects of paters' doula touch during labor on paternal attachment and role confidence to neonate and couple attachment. Methods: The participants were 60 couples of laboring woman and her husband who were hospitalized from May 2010 to december 2010. Among 60 couples, 30 couples were assigned to an experimental group and the other 30 couples to a control group. All of them had any other complications of pregnancy. During one month, the paters' duola touch was applied to the experimental group of 32~42 gestation once a week and be in labor. Data were analyzed using SPSS/WIN 14.0 Win program. Results: The scores for role confidence to neonate and couple attachment in the experimental group were significantly higher than those for the control group. The paternal attachment score of the experimental group was higher than that of the control group, but it was not significant. Conclusion: From these findings, it is concluded that paters'doula touch during pregnancy and labor can be helpful to improve attachment to the neonate and his/her spouse.

• Clinical and Experimental Pediatrics
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• 제46권2호
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• pp.192-194
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• 2003

가정분만으로 태어난 후 생후 6일된 신생아에서 전신성 강직-간대 경련이 발생하여 내원한 환아에서, 병력과 진찰 소견상 신생아 파상풍으로 진단하여 근이완제 및 인공환기요법으로 치료한 1례를 경험하였기에 보고하는 바이다.

• Child Health Nursing Research
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• 제3권1호
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• pp.5-18
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• 1997

This study was designed to compare the educational needs of the mothers of LBW infant and normal neonate for development of the educational program. The subjects for this study were 37 mothers of LBW and 60 mothers of normal neonate at 3 general hospitals in Seoul and Inchon. The data were collected during the period from July to November, 1996. The Educational Need for Infant Care was measuerd by questionnaire that has developed by researchers. The data were analized by descriptive statistics, non-paired t-test, ANOVA, ANCOVA using SPSS PC/sup +/. The results were as fellow : 1) The educational needs of the mothers of LBW infant were higher than the mothers of normal neonate(F=14.50, P=.000). 2) There were significant associations between the educational needs of the mothers of LBW infant and delivery history(nullipara. or multipara. ) and caregiver of postpartum(t:-2.08, P=.045 ; F=3.94, P=.0282). 3) There were significant associations between the educational needs of the mothers of normal neonate and numbers of children and caregiver of postpartum(F=5.53, P=.0064 ; F=3.22, P=.0480) .4) The educational need by content was signs, symptomes and management of disorders (i. g. cyanosis, seizure, fever, vomiting etc.) were higher than general care of baby(i. g. feeding, diaper change etc.) at two groups. In conclusion, when the nurses teaching the method of infant care to mother, there sholud be in consideration of delivery history and caregiver of postpartum. Also, its educational contents must be Included of signs, symptomes and management of disorders.

• Advances in pediatric surgery
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• 제20권2호
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• pp.33-37
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• 2014

Purpose: The purpose of this study was to compare the diagnostic accuracy of the non-invasive diagnostic methods and rectal suction biopsy for the detection of Hirschsprung disease (HD). Methods: We reviewed diagnostic methods and results retrospectively in patients who underwent anorectal manometry, barium enema and rectal suction biopsy for the diagnosis of HD at Asan Medical Center from January 2000 to December 2012. Results: There were 97 patients (59 neonates and 38 infants) in the study period. The overall accuracy of anorectal manometry for the diagnosis of HD was 71.1% and its sensitivity was 51.4% (48.1% in neonate and 62.5% in infant, respectively) and its overall specificity was 82.3% (81.3% in neonate and 83.3% in infant, respectively). The Overall accuracy of barium enema was 66.0% (72.8% in neonate and 55.3% in infant, respectively) and specificity of barium enema was 53.2% (56.3% in neonate and 50.0% in infant, respectively). These results were lower than those of anorectal manometry. The overall sensitivity of barium enema was 88.6% (92.6% in neonate and 75.0% in infant, respectively) and it was higher than the sensitivity of anorectal manometry. Histological studies confirmed HD in 35 patients, in one of whom the suction biopsy showed negative finding. Conclusion: Accuracy of non-invasive methods for diagnosis of HD in our study is lower than those in previous study, so we need to improve the quality of diagnostic tools in our hospital. We conclude that the rectal suction biopsy is the most accurate test for diagnosing HD, so the biopsy to confirm the diagnosis of the HD is very important.

• Clinical and Experimental Pediatrics
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• 제52권8호
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• pp.851-855
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• 2009

Death is not only a medical problem; it is also an ethical problem. When doctors face a dying neonate, their knowledge of bioethics and the opinions of ethical specialists and religious leaders are helpful for them and the family of the dying baby. In recent years, due to the increase of surviving babies who have suffered from severe illness, those born too small or too early, and those with severe anomalies in neonatal intensive care unit (NICU), we have met with complicated bioethical problems frequently. To lessen the burdens of doctors and the parents of the dying baby, I reviewed medical, ethical and religious articles about bioethics in adult death. My suggestions are listed as follows: 1) regular bioethical education and activation of bioethical committees in NICU, 2) a well-controlled nationwide database, 3) a hospice unit space and programs for dying baby in NICU, and 4) social support for pregnant women and financial support for the NICU.