• The Korean Journal of Ecology
• /
• v.19 no.2
• /
• pp.151-163
• /
• 1996

To investigate the early symptoms of light-chilling, alterations of chlorophyll fluorescence transients were monitored in cucumber (Cucumis sativus L. cv. Ilmichungjang) leaves. During 24 h chilling, decreases in (Fv)m/Fm, qE and qQ, and an increase in Fo were observed. The chilling effects were not recovered at room temperature, and a significant increase in Fo was observed during the recovery period. After 6 h chilling, ‘dip’(D) level of the transients became obscure, and the negative slope after ‘peak’(P) disappeared. The first derivative (dFv/dt) of the fast fluorescence rise curve was used to obtain more accurate information about the changes in the transients. The maximal rate of the fluorescence increase in the D-p rise curve (Fr) has been the most frequently used chilling stress indicator. However, a correct value of Fr could not be measured when the D level became obscure. This problem was overcome by introducing a new indicator, HFr (dFv/dt at Fv = 1/2 (Fv)m), and HFr gave very similar values to Fr. To monitor the changes in curvature around D level, another new parameter, ${\Delta}S$(D-Fr), was also introduced. These three parameters decreased very sensitively during light-chilling. In addition, increases in these parameters were observed during the first 2 h chilling, but this increase in Fr was also observed in pea leaf discs dark-chilled for 15 min, suggesting that this very early change is a common response to chilling in both pea and cucumber leaves. Quenching coefficients were also very sensitive to chilling, especially qE. Discussion on the usage of these parameters as chilling stress indicators is given in the text.

• Asian Pacific Journal of Cancer Prevention
• /
• v.13 no.10
• /
• pp.5161-5165
• /
• 2012

Background: There are few molecular markers known to predict cholangiocarcinoma (CCA) prognosis. Smad7 has a certain relationship with epithelial-mesenchymal transition (EMT), but its relevance to CCA in unclear. Therefore expression and clinical significance of Smad7 in CCA was the focus of this study. Methods: Expression of Smad7, E-cadherin and vimentin was assessed in 41 patients with CCA by immunohistochemistry and analyzed for associations with clinical parameters. Results: Smad7 and vimentin expression in the CCA tissue was dramatically higher than that in adjacent tissues. In addition, Smad7, vimentin and E-cadherin expression was significantly associated with CCA lymph node metastasis and perineural invasion($P{\leq}0.05$), but not other factors, such as gender, age, tumor location, tumor type and tumor differentiation degree (P>0.05). The overall survival and relapse-free survival rate was significantly higher in patients with negative Smad7 expression than those with positive Smad7 expression. Conclusion: EMT phenomena may occur in the process of CCA invasion and metastasis. Smad7, which was highly expressed in CCA, may be considered to be one feedback regulator in late stages and could have potential as a prognostic indicator for clinical assessment.

• Journal of Microbiology and Biotechnology
• /
• v.14 no.2
• /
• pp.290-296
• /
• 2004

The effects of potassium, sodium, calcium, magnesium, and iron on cyanobacterial bloom potentials were investigated in Daechung Reservoir, Korea. Potassium showed the highest correlation with the cyanobacterial cell number (r=0.487, P<0.05) and phycocyanin concentration (r=0.499, P<0.05). However, it was not likely that the potassium had directly affected the bloom formation, because the variations of its concentration were not significantly large. In contrast, the Fe concentration fluctuated drastically and exhibited a negative correlation with the cyanobacterial cell number (r=- 0.388, P<0.1) and phycocyanin concentration (r=-0.446, P<0.05). Accordingly, the K:Fe atomic ratio would appear to reflect the extent of cyanobacterial bloom more precisely than K or Fe alone. The K:Fe ratio specifically correlated with cyanobacterial percentage, the cyanobacterial cell number and phycocyanin concentration (r=0.840, P<0.001; r=0.416, P<0.05; r=0.522, P<0.01, respectively). With the K:Fe atomic ratio of over 200, the chlorophyll-a concentration, cyanobacterial cell number, and phycocyanin concentration exceeded $10\mu$g $1^{-1}$20,000 cells $ml^{-1}$, and 20 pM, respectively, the general criteria of eutrophic water.

• YAKHAK HOEJI
• /
• v.49 no.3
• /
• pp.217-224
• /
• 2005

Human Immunodeficiency Virus type 1 (HIV-l) based lentivirus vector has demonstrated great potential as gene therapy vectors mediating efficient gene delivery and long-term transgene expression in both dividing and nondividing cells. However, for clinical studies it must be confirmed that vector preparations are safe and not contaminated by replication competent lentivirus (RCL) related to the parental pathogenic virus, HIV-l. In this study, we would like to establish the method for titration and RCL detection of lentivirus vector. The titration was determined by vector expression containing the green fluorescent protein, GFP in transduced cells. The titer was $1{\times}10^7$ Transducing Unit/ml in the GFP expression assay and $8.9{\times}10^7$ molecules/ml in the real-time PCR. Also, for the detection of RCL, we have used a combination method of PCR and p24 antigen detection. First, PBS/psi and VSV-G region in the genomic DNA of transduced cells was detected by PCR assay. Second, transfer and expression of the HIV-1 gag gene was detected by p24 ELISA. In an attempt to amplify any RCL, the transduced cells were cultured for 3 weeks (amplification phase) and the supernatant of amplified transduced cell was used for the second transduction to determine whether a true RCL was present (indicator phase). Analysis of cells and supernatant at day 6 in indicator phase were negative for PBS/psi, VSV-G, and p24 antigen. These results suggest that they are not mobilized and therefore there are no RCL in amplification phase. Thus, real-time PCR is a reliable and sensitive method for titration and RCL detection of lentivirus vector.

• The Journal of Bigdata
• /
• v.7 no.1
• /
• pp.89-97
• /
• 2022

Various efforts are needed to prevent accidents because ship collisions can cause various negative situations such as economic losses and casualties. Therefore, research to prevent accidents is being actively conducted, and in this study, new leading indicators for preventing ship collision accidents is proposed. In previous studies, the risk of collision was expressed in consideration of the distance between ships in a specific sea area, but there is a disadvantage that a new model needs to be developed to apply this to other sea areas. In this study, the density-based ship domain DESD (Density-based Empirical Ship Domain) including the environment and operating characteristics of the sea area was defined using AIS (Automatic Identification System) data, which is ship operation information. Deep clustering is applied to two-dimensional DESDs created for each sea area to cluster the seas with similar operating environments. Through the analysis of the relationship between clustered sea areas and ship collision accidents, it was statistically tested that the occurrence of accidents varies by characteristic of each sea area, and it was proved that DESD can be used as a leading indicator of accidents.

• International Journal of Computer Science & Network Security
• /
• v.24 no.7
• /
• pp.73-78
• /
• 2024

The present world is full of challenges and opportunities for the women at work. Every woman works hard for academic growth and professional development. For the same reason they also face challenges every day such as stresses, tensions, problems and competitions. When these factors exceed above the strengths, the capabilities of a woman start drowning under stress. Stress is generally thought negative characteristic, but stress or tension maintained up to some level is necessary and effective towards work done at job. This paper covers the study relationship between job stressor and female teacher's performance. The study was carried out in Government Colleges of District Hyderabad, Pakistan. The teacher student relationship was selected as a stressor for female teachers. The study is descriptive in nature followed by method of correlation. A five point "Likert Scale" was developed to collect the data from the sample size of 158 college teachers drawn randomly and the collected data was assessed quantitatively. The relationship between the "teacher student" (as an indicator of job stress) and "female teachers' performance" was calculated by Pearson Correlation formula. The findings of the study show the significant correlations between the teacher student relationship and job stress in female teachers' performance where it is found that the stressor caused the poor Physical and Mental health of female teachers working in colleges.

• Science of Emotion and Sensibility
• /
• v.20 no.3
• /
• pp.13-22
• /
• 2017

Emotion has a direct influence such as decision-making, perception, etc. and plays an important role in human life. For the convenient and accurate recognition of high-arousal negative emotion, the purpose of this paper is to design an algorithm for analysis using the bio-signal. In this study, after two emotional induction using the 'normal' / 'fear' emotion types of videos, we measured the Galvanic Skin Response (GSR) signal which is the simple of bio-signals. Then, by decomposing Tonic component and Phasic component in the measured GSR and decomposing Skin Conductance Very Slow Response (SCVSR) and Skin Conductance Slow Response (SCSR) in the Phasic component associated with emotional stimulation, extracting the major features of the components for an accurate analysis, we used a discrete wavelet transform with excellent time-frequency localization characteristics, not the method used previously. The extracted features are maximum value of Phasic component, amplitude of Phasic component, zero crossing rate of SCVSR and zero crossing rate of SCSR for distinguishing high-arousal negative emotion. As results, the case of high-arousal negative emotion exhibited higher value than the case of low-arousal normal emotion in all 4 of the features, and the more significant difference between the two emotion was found statistically than the previous analysis method. Accordingly, the results of this study indicate that the GSR may be a useful indicator for a high-arousal negative emotion measurement and contribute to the development of the emotional real-time rating system using the GSR.

• Journal of Food Hygiene and Safety
• /
• v.16 no.3
• /
• pp.241-246
• /
• 2001

Distribution of pathogenic vibrios in the seawater of live fish tank and effect of environmental factors on their existence were investigated by collecting samples from fish markets and restaurants in 6 different cities. Pathogenic vibrios and coliforms were determined by using the most probable number (MPN) procedure, and aerobic plate count was enumerated by the standard pour plate method. No Vibrio chulerae O1 was detected in all the samples tested. Detection rates of V. cholerae non-O1, V. parahaemolyticus and V. vulnificus in all the smaples tested were 7.7%, 69.2% and 23.1%, respectively. Water temperature and trubidity of the seawater measured were higher in the pathogenic vibrios positive samples than in those negative samples. However, higher salinity and pH were shown in the pathogenic vibrios negative samples than in positive samples. The aerobic plate counts and MPN or total and focal coliforms in the seawater were higher in the presence of pathogenic vibrios than in the absence of pathogenic vibrios. In this study, the presence of pathogenic vibrios in the seawater tested was closely related with other physiochemical parameters and populations of coliforms, indicators far food safety.

• ALGAE
• /
• v.19 no.4
• /
• pp.329-337
• /
• 2004

Monthly variations of phytoplankton communities were conducted to the investigation at five stations in mid and lower parts of the Nakdong River from December 1995 to November 1996. The phytoplankton communities were identified a total 456taxa which were composed of 136 genera, 427 species, 27 varieties and 2 forma. The standing crops of phytoplankton communities ranged from 2.7 to 52.8 (${\times}$10³)cells·ml$^{-1}$ during the investigation periods. Bacillariophyceae was the dominant classes at all stations. The dominant species were 7taxa and the standing crops of these ranged from 18.3 to 95.1% of the standing crops of phytoplankton communities. The dominant species in the mid parts (st. 1, 2) of this river system were benthic species such as Navicula gregaria, N. viridula var. rostellata and Nitzschia palea, however it was planktonic species (Stephan discus hantzschii, Golenkinia radiata) and meroplanktonic species (Aulacoseira ambigua, A. granulata) in the lower parts (st. 3, 4, 5). The seasonal variations of the dominant species in the lower parts were appeared to the planktonic species (S. hantzschii) from winter to spring, and were the meroplanktonic species (A. ambigua and A. granulata) in from summer to fall. The lower parts of the Nakdong river were entropic states because the dominant species were composed A. ambigua, A. granulate and S. hantzschii which were indicators of entropic water quality. The interrelationships between total standing crops and environmental factors(water temperature, pH, NH$_4$, NO$_2$, NO$_3$ and PO$_4$) were low positive or negative coefficients. S. hantzschii had low positive or negative relations with water temperature, NH$_4$, NO$_2$, NO$_3$ and PO$_4$. As the result, the variations of standing crops of phytoplankton were not caused by a single factor but controlled by the complex factors.

• Asian Pacific Journal of Cancer Prevention
• /
• v.16 no.18
• /
• pp.8155-8161
• /
• 2016

The prognostic significance of AgNOR proteins in stage II-III rectal cancers treated with chemoradiotherapy was evaluated. Silver staining was applied to the $3{\mu}m$ sections of parafin blocked tissues from 30 rectal cancer patients who received 5-FU based chemoradiotherapy from May 2003 to June 2006. The microscopic displays of the cells were transferred into the computer via a video camera. AgNOR area (nucleolus organizer region area) and nucleus area values were determined as a nucleolus organizer regions area/total nucleus area (NORa/TNa). The mean NORa/TNa value was found to be $9.02{\pm}3.68$. The overall survival and disease free survival in the high NORa/TNa (>9.02) patients were 52.2 months and 39.4 months respectively, as compared to 100.7 months and 98.4 months in the low NORa/TNa (<9.02) cases. (p<0.001 and p<0.001 respectively). In addition, the prognosis in the high NORa/TNa patients was worse than low NORa/TNa patients (p<0.05). In terms of overall survival and disease-free survival, a statistically significant negative correlation was found with the value of NORa/TNa in the correlations tests. Cox regression analyses demostrated that overall survival and disease-free survival were associated with lymph node status (negative or positive) and the NORa/TNa value. We suggest that two-dimensional AgNOR evaluation may be a safe and usable parameter for prognosis and an indicator of cell proliferation instead of AgNOR dots.