• Title/Summary/Keyword: near infrared reflectance spectroscopy

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EVALUATION OF RAPID DETERMINATION OF PHOSPHORUS IN SOILS BY NIR SPECTROSCOPY

  • Ryu, Kwan-Shig;Kim, Book-Jin;Park, Jin-Sook
    • Proceedings of the Korean Society of Near Infrared Spectroscopy Conference
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    • 2001.06a
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    • pp.1072-1072
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    • 2001
  • The purpose of this research is to evaluate rapid determination of phosphorus in soils using NIR spectroscopy. The soil samples from the fields subject to different crops and land-use in Kyeongbook province, Korea were used to make the calibration and validation of the calibration set estimating phosphorus in soil. The NIR reflectance was scanned at 2nm intervals from 1100 to 2500nm with an InfraAlyzer 500 (Bran+Luebbe Co.). Various regression analyses were used to evaluate a NIRS method for determination of phosphorus in the soil. NIR absorption approach requires many soil samples to obtain optimal prediction. Applicability of NIR spectra technique may allow for the analysis of available soil phosphorus rapidly as well as total component within a few seconds.

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Measurement of human skin moisture in the near infrared region from 1100 nm to 2200 nm by using a portable system

  • Suh, Eun-Jung;Ahn, Jhii-Weon;Woo, Young-Ah;Kim, Hyo-Jin
    • Proceedings of the PSK Conference
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    • 2003.04a
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    • pp.290.2-290.2
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    • 2003
  • Skin Moisture is an important factor in skin health. Measurement of moisture content can provide diagnostic information on the condition of skin. In this study, a photo diode array type near infrared spectroscopy was used to determine skin moisture. Diffuse reflectance spectra were collected and transformed to absorbance using 1-nm stepsize over the wavelength range of 1100 nm to 2200 nm. (omitted)

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PRELIMINARY STUDY ON NIR SPECTRA OF RETROGRADED STARCH

  • Terazawa, Yoko;Maekawa, Takaaki;Kawano, Sumio
    • Proceedings of the Korean Society of Near Infrared Spectroscopy Conference
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    • 2001.06a
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    • pp.1515-1515
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    • 2001
  • Retrogradation of starch is one of important quality indexes for food based on starch such as rice. Therefore, in this research, possibility of near infrared spectroscopy to determine the degree of the retrogradation was examined. The degree of the retrogradation was indicated as the degree of geratinization analyzed by BAP(-amylase-pullulanase) method. 20 samples which have a wide range of the degree of the retrogradation were prepared and the NIR spectra of the samples were measured in reflectance mode with the NIR Systems 6500. In the correlation plots calculated from the 2nd derivative values of the MSC treated spectra and the degree of the geratinization, the major negative peaks of 1544 nm and 2258 nm, and the major positive peaks of 1460 nm, 1602 nm, 1766 nm and 2136 nm could be observed, indicating that NIR absorption at the positive peak wavelengths became strong while the absorption at the negative peak wavelengths became weak as the degree of the retrogradation increased. Because there is negative correlation between the degree of the retrogradation and the degree of the geratinization. As a result of MLR using the 2nd derivative values of the MSC treated spectra and the degree of the geratinization, good calibration equation which include 2258 nm as the first wavelength and 1764 nm as the second one could be obtained, indicating that NIR spectroscopy has a possibility to detect the retrogradation of starch. In order to find the assignment of the bands observed in the correlation plots, further study may be needed.

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Predicting Calcium and Phosphorus Concentrations in Imported Hay by near Infrared Reflectance Spectroscopy (근적외선분광법을 이용한 수입건초의 Ca과 P 함량 예측)

  • Lee, Bae Hun;Kim, Ji Hye;Oh, Mirae;Lee, Ki Won;Park, Hyung Soo
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.41 no.1
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    • pp.29-34
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    • 2021
  • Near infrared reflectance spectroscopy (NIRS) is routinely used for the determination of nutrient components of forages. However, little is known about the impact of sample preparation and wavelength on the accuracy of the calibration to predict minerals. This study was conducted to assess the effect of sample preparation and wavelength of near infrared spectrum for the improvement of calibration and prediction accuracy of Calcium (Ca) and Phosphorus (P) in imported hay using NIRS. The samples were scanned in reflectance in a monochromator instrument (680-2,500 nm). Calibration models (n = 126) were developed using partial least squares regression (PLS) based on cross-validation. The optimum calibrations were selected based on the highest coefficients of determination in cross validation (R2) and the lowest standard error of cross-validation (SECV). The highest R2 and the lowest SECV were obtained using oven-dry grinded sample preparation and 1,100-2,500 nm wavelength. The calibration (R2) and SECV were 0.99 (SECV: 468.6) for Ca and 0.91 (SECV: 224.7) for P in mg/kg DM on a dry weight, respectively. Results of this experiment showed the possibility of NIRS method to predict mineral (Ca and P) concentration of imported hay in Korea for routine analysis method to evaluate the feed value.

Vitamin C Tablet Assay by Near -Infrared Reflectance spectrometry

  • Kargosha, Kazem;Ahmadi, Hamid;Nemati, Nader
    • Proceedings of the Korean Society of Near Infrared Spectroscopy Conference
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    • 2001.06a
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    • pp.4111-4111
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    • 2001
  • When a drug is prepared in a tablet, the active component represents only a small portion of the dosage form. The other components of the formulation include materials to assist in the dissolution, antioxidants, coloring agents and bulk fillers. The tablets are tested using approved testing methods usually involving separation and subsequent quantification of the active component. Tablets may also be tested by near-Infrared Reflectance spectrometry (NIRS). In the present study, based on NIRS and multivariate calibration methods, a novel and precise method is developed for direct determination of ascorbic acid in vitamin C tablet. Two different tablet formulations were powdered in three different sizes, 63-125 ${\mu}{\textrm}{m}$, and examined. Spectral region of 4750-4950 $cm^{-1}$ / was used and optimized for quantitative operations. Partial least squares (PLS) and multiple linear regression (MLR) methods were performed for this spectral region. The results of optimized PLS and MLR methods showed that reproducibility increase with decreasing grain size and standard error of calibration (SEP) of less than 1% w/w of ascorbic acid and a correlation coefficient of 0.998 can be achieved. The PLS method showed better results than MLR. Seven overdose and underdose samples (prepared in the laboratory to match marketed products) were tested by proposed and iodometric standard methods. A correlation between NIRS predicted ascorbic acid values and iodomet.ic values was calculated ($R^2$=0.9950). Finally, the direct analysis of individual intact tablets in their unit-dose packages (Blistering in aluminum and PVC foils) obtained from market were also carried out and a correlation coefficient of 0.9989 and SEP of 0.931% w/w of ascorbic acid were achieved.

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THE USE OF NEAR INFRARED REFLECTANCE SPECTROSCOPY(NIRS) TO PREDICT CHEMICAL COMPOSITION ON MAIZE SILAGE

  • D.Cozzolino;Fassio, A.;Mieres, J.;Y.Acosta
    • Proceedings of the Korean Society of Near Infrared Spectroscopy Conference
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    • 2001.06a
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    • pp.1610-1610
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    • 2001
  • Microbiological examination of silage is of little value in gauging the outcome of silage, and so chemical analysis is more reliable and meaningful indicator of quality. On the other hand chemical assessments of the principal fermentation products provide an unequivocal basis on which to judge quality. Livestock require energy, protein, minerals and vitamins from their food. While fresh forages provide these essential items, conserved forages on the other hand may be deficient in one or more of them. The aim of the conservation process is to preserve as many of the original nutrients as possible, particularly energy and protein components (Woolford, 1984). Silage fermentation is important to preservation of forage with respect of feeding value and animal performance. Chemical and bacteriological changes in the silo during the fermentation process can affect adversely nutrient yield and quality (Moe and Carr, 1984). Many of the important chemical components of silage must be assayed in fresh or by extraction of the fresh material, since drying either by heat or lyophilisation, volatilises components such as acids or nitrogenous components, or effects conversion to other compounds (Abrams et al., 1987). Maize silage dorms the basis of winter rations for the vast majority of dairy and beef cattle production in Uruguay. Since nutrient intake, particularly energy, from forages is influenced by both voluntary dry matter intake and digestibility; there is a need for a rapid technique for predicting these parameters in farm advisory systems. Near Infrared Reflectance Spectroscopy (NIRS) is increasingly used as a rapid, accurate method of evaluating chemical constituents in cereals and dried forages. For many years NIRS was applied to assess chemical composition in dry materials (Norris et al., 1976, Flinn et al., 1992; Murray, 1993, De Boever et al., 1996, De la Roza et al., 1998). The objectives of this study were (1) to determine the potential of NIRS to assess the chemical composition of dried maize samples and (2) to attempt calibrations on undried samples either for farm advisory systems or for animal nutrition research purposes in Uruguay. NIRS were used to assess the chemical composition of whole - plant maize silage samples (Zea mays, L). A representative population of samples (n = 350) covering a wide distribution in chemical characteristics were used. Samples were scanned at 2 nm intervals over the wavelength range 400-2500 nm in a NIRS 6500 (NIRSystems, Silver Spring, MD, USA) in reflectance mode. Cross validation was used to avoid overfitting of the equations. The optimum calibrations were selected on the basis of minimizing the standard error of cross validation (SECV). The calibration statistics were R$^2$ 0. 86 (SECV: 11.4), 0.90 (SECV: 5.7), 0.90 (SECV: 16.9) for dry matter (DM), crude protein (CP), acid detergent fiber (ADF) in g kg$\^$-1/ on dry matter, respectively for maize silage samples. This work demonstrates the potential of NIRS to analyse whole - maize silage in a wide range of chemical characteristics for both advisory farm and nutritive evaluation.

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OPTIMISING CALIBRATION TRANSFER TO MEASURE DEGRADABILITY PARAMETERS OF HAYS AND DEHYDRATED FORAGES

  • Andueza, Donato;Munoz, Fernando;Martinez, Adela;De La Roza, Begona
    • Proceedings of the Korean Society of Near Infrared Spectroscopy Conference
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    • 2001.06a
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    • pp.1268-1268
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    • 2001
  • The availability of in vivo and in sacco degradability values are limited because those methods require work with fistulated animals and are rather complicated, labour intensive and expensive. That is to say, the dynamics and logistics of the methodology result in considerable work, due to limitations on the amount of samples, number of bags that can be placed in an animal and different time intervals to perform kinetic studies. Therefore, a simpler method is necessary to estimate the degradation characteristics of the feed. In this way, near infrared reflectance spectroscopy has been used to predict degradation characteristics of forages. In other hand, the possibility of achieving successful transfer of spectra and equations between instruments is closely related. The objective of this study was to confirm the potential of NIR to optimize work conditions to avoid duplicated efforts in collaborative trials on animal feeds evaluation between research institutions. For this purpose, one set with forty hays and dehydrated forages samples from SERIDA and ten samples with the same characteristics from SIA, were be used to create a spectral database. A calibration was developed using samples from degradation essays made in SERIDA to predict dry matter and crude protein degradability. With the addition of five samples from SIA in original calibration set, the effect of different origin and location was compensated.

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Development of Models for the Prediction of Domestic Red Pepper (Capsicum annuum L.) Powder Capsaicinoid Content using Visible and Near-infrared Spectroscopy

  • Lim, Jongguk;Mo, Changyeun;Kim, Giyoung;Kim, Moon S.;Lee, Hoyoung
    • Journal of Biosystems Engineering
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    • v.40 no.1
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    • pp.47-60
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    • 2015
  • Purpose: The purpose of this study was to non-destructively and quickly predict the capsaicinoid content of domestic red pepper powders from various areas of Korea using a pungency measurement system in combination with visible and near-infrared (VNIR) spectroscopic techniques. Methods: The reflectance spectra of 149 red pepper powder samples from 14 areas of Korea were obtained in the wavelength range of 450-950 nm and partial least squares regression (PLSR) models for the prediction of capsaicinoid content were developed using area models. Results: The determination coefficient of validation (RV2), standard error of prediction (SEP), and residual prediction deviation (RPD) for the capsaicinoid content prediction model for the Namyoungyang area were 0.985, ${\pm}2.17mg/100g$, and 7.94, respectively. Conclusions: These results show the possibility of VNIR spectroscopy combined with PLSR models in the non-destructive and facile prediction of capsaicinoid content of red pepper powders from Korea.

IDENTIFICATION OF GEOGRAPHICAL ORIGIN AND VARIETY OF GREEN COFFEE BY NIR

  • Nzabonimpa, Rukundo;Prodolliet, Jacques;Vouilloz, Annick
    • Proceedings of the Korean Society of Near Infrared Spectroscopy Conference
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    • 2001.06a
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    • pp.1151-1151
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    • 2001
  • The international coffee trade is conducted almost exclusively with green coffee. The main coffee producing countries include Brazil, Columbia, Indonesia, Mexico and the Ivory Coast. About 99 % of the coffee grown throughout the world belong to two coffee plant varieties that are commonly known as Arabica and Robusta. The quality of green coffee can be assessed according to several ISO standards (1,2,3,4,5). However, no official international standards for the authenticity of green coffee have been issued. It is important to know the country of origin of the coffee for the purposes of fair international trade. The geographic origin of the coffee is often stated on the label of coffee products such as speciality roasted and soluble coffees. Near Infrared Spectroscopy (NIR) is an accepted technique for quantitative analysis of various parameters in routine QC analysis of food products. It would appear to be a promising candidate as a tool for identification of green coffee origin and numerous feasibility studies have appeared in the literature on its use for soluble, roasted and green coffee variety identification as well as identification of arabica or robusta coffees. NIR spectrophotometers when configured in the reflectance mode are able to perform a complete profile of the NIR spectrum on whole beans. The data can then be interpreted by discriminant chemometrics data analysis. This is the approach used in the present study.

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Application of Near Infrared Spectroscopy for Nondestructive Evaluation of Nitrogen Content in Ginseng

  • Lin, Gou-lin;Sohn, Mi-Ryeong;Kim, Eun-Ok;Kwon, Young-Kil;Cho, Rae-Kwang
    • Proceedings of the Korean Society of Near Infrared Spectroscopy Conference
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    • 2001.06a
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    • pp.1528-1528
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    • 2001
  • Ginseng cultivated in different country or growing condition has generally different components such as saponin and protein, and it relates to efficacy and action. Protein content assumes by nitrogen content in ginseng radix. Nitrogen content could be determined by chemical analysis such as kjeldahl or extraction methods. However, these methods require long analysis time and result environmental pollution and sample damage. In this work we investigated possibility of non-destructive determination of nitrogen content in ginseng radix using near-infrared spectroscopy. Ginseng radix, root of Panax ginseng C. A. Meyer, was studied. Total 120 samples were used in this study and it was consisted of 6 sample sets, 4, 5 and 6-year-old Korea ginseng and 7, 8 and 9-year-old China ginseng, respectively. Each sample set has 20 sample. Nigrogen content was measured by electronic analysis. NIR reflectance spectra were collected over the 1100 to 2500 nm spectral region with a InfraAlyzer 500C (Bran+Luebbe, Germany) equipped with a halogen lapmp and PbS detector and data were collected every 2 nm data point intervals. The calibration models were carried out by multiple linear regression (MLR) and partial least squares (PLS) analysis using IDAS and SESAME software. Result of electronic analysis, Korean ginseng were different mean value in nitrogen content of China ginseng. Ginseng tend to generally decrease the nitrogen content according as cultivation year is over 6 years. The MLR calibration model with 8 wavelengths using IDAS software accurately predicted nitrogen contents with correlation coefficient (R) and standard error of prediction of 0.985 and 0.855%, respectively. In case of SESAME software, the MLR calibration with 9 wavelength was selected the best calibration, R and SEP were 0.972 and 0.596%, respectively. The PLSR calibration model result in 0.969 of R and 0.630 of RMSEP. This study shows the NIR spectroscopy could be applied to determine the nitrogen content in ginseng radix with high accuracy.

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