• Korean Journal of Plant Resources
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• v.11 no.3
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• pp.301-306
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• 1998

In order to improve cultivation technuque of C. lanceolata, natural ABA was treated with foliar application periodically during differenctitation of node. The higher is concentration and the earier its foliar application was, the shoter plant height was. Especially, when $10mg;.L{-1}$ of ABA was treated at differentiated stage of 3rd node, plant height was inhibited to 60% of control. But leaf length, leaf width, and number of branches have no significant differnence in comparison wiht control. The fresh weight of subterranean part was similar to control independent of treat-time in the case of $10mg;.L{-1}$. When 1,5 and $10mg;.L{-1}$ of ABA were treated at initial differentiated node stage, plant height inhibited to 20~30% of control, but subterranean part was similar to control. All treatement showed slight antitumor activity by the P388 cytotoxic screening test.

• Proceedings of the Korean Society of Crop Science Conference
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• 2017.06a
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• pp.90-90
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• 2017

Regulation of seed dormancy is important in many grains to prevent pre-harvest sprouting. To identify and understand the gene related to seed dormancy regulation, we have screened for viviparous phenotypes of rice mutant lines generated by insertion of Ds transposon in a Korean Japonica cultivar (Dongjin) background. One of the mutants, which represented viviparous phenotype, was selected for further seed dormancy regulation studies and designated dor1. The dor1 mutant has single Ds insertion in the second exon of OsDor1 gene encoding glycine-rich protein. The seeds of dor1 mutant showed a higher germination potential and reduced abscisic acid (ABA) sensitivity compared to wild type Dongjin. Over-expression of Dor1 complements the viviparous phenotype of dor1 mutant, indicating that Dor1 function in seed dormancy regulation. Subcellular localization assay of Dor1-GFP fusion protein revealed that the OsDor1 protein mainly localized to membrane and the localization of OsDOR1 was influenced by presence of a giberelin (GA) receptor OsGID1. Further bimolecular fluorescence complementation (BiFC) analysis indicated that OsDOR1 interact with OsGID1. The combined results suggested that OsDOR1 regulates seed dormancy by interacting with OsGID1 in GA response. Additionally, expression of OsDOR1 partially complemented the cold sensitivity of Escherichia coli BX04 mutant lacking four cold shock proteins, indicating that OsDOR1 possessed RNA chaperone activity.