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Verification of Anti-Inflammatory Effects of Strawberry (Fragaria x ananassa var. 'Seolhyang') Stems on Macrophages Stimulated by Lipopolysaccharides (LPS로 자극된 대식세포에 대한 딸기 줄기의 항염증 효능 검증)

  • Dan-Hee Yoo;In-Chul Lee
    • Microbiology and Biotechnology Letters
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    • v.51 no.3
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    • pp.280-288
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    • 2023
  • In strawberry farming, most parts of strawberry stems but the fruit have been dumped. Therefore, this study attempted to investigate the antioxidant and anti-inflammatory effects of strawberry stems which are thrown away after farming. For this, strawberry stem extracts were obtained, using hot water and 70% ethanol. First, total polyphenol contents of the hot water and ethanol extract were checked (265.4 ± 0.12 mg TAE/100 g, 503.88 ± 0.2 mg TAE/100 g). For analysis of antioxidant activities, electron donating ability (EDA) and 2,2'-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging activity were measured. Both extracts increased in a dose-dependent fashion, and similar effects with vitamin C (control group) were confirmed. In terms of cell viability of the hot water and ethanol extracts of strawberry stems, 'RAW 264.7' was 99% or higher at 500 ㎍/ml. In addition, cell experiments were conducted at 50, 100 and 500 ㎍/ml where cell viability is above 99%. In terms of inhibition of the inflammatory mediator 'nitric oxide (NO)', the hot water and ethanol extracts of strawberry stems were 37.9% and 38.8% respectively, confirming the inhibition of NO production. To check anti-inflammatory activities, protein and mRNA expressions of 'iNOS' and 'COX-2' were measured, using RAW 264.7. Compared to the LPS group, the protein expression of the inflammatory mediators was inhibited in the hot water and ethanol extract-treated groups. The above results confirmed that the hot water and ethanol extracts of strawberry stems are valuable as natural substances with antioxidant and anti-inflammatory activities.

Study on Geological Distribution of Fluorine in Forest Aggregate within Korea (산림골재 내 불소의 지질학적 분포 연구)

  • Yeong-Il Jeong;Kun-Ki Kim;Soon-Oh Kim;Sang-Woo Lee;Jin-Young Lee
    • Economic and Environmental Geology
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    • v.57 no.2
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    • pp.233-241
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    • 2024
  • This study was conducted to investigate the geological distribution characteristics of fluorine in rocks, which can be a major resource of forest aggregates in Korea. Samples of forest aggregates were collected from 224 sites in 22 cities and counties for this study. The national background concentration was 344 mg/kg, which was significantly lower than the average fluorine concentration of crustal, which was 625 mg/kg, and slightly higher than the average fluorine concentration of world soil, which was 321 mg/kg. In terms of region and tectonic structure, fluorine concentrations were investigated to be highest in Gyeonggi-do(394 mg/kg) and Gyeonggi massif(396 mg/kg), respectively. The concentration distribution by the origin of the parent rock was in the order of metamorphic rock(362 mg/kg) > sedimentary rock(354 mg/kg) > igneous rock(328 mg/kg), and the concentration distribution by geologic ages was the highest in the Paleozoic at 394 mg/kg. The concentration distribution by rock types was in the order of diorite(515 mg/kg) > gneisses(377 mg/kg) > schists(344 mg/kg) > phyllite(306 mg/kg) > granites(305 mg/kg) > quartz porphyry(298 mg/kg). Consequently, it is speculated that gneisses and schists, Precambrian metamorphic rocks in the Gyeonggi massif that forms the crust of Gyeonggi-do, contain high fluorine concentrations.

Contactless Data Society and Reterritorialization of the Archive (비접촉 데이터 사회와 아카이브 재영토화)

  • Jo, Min-ji
    • The Korean Journal of Archival Studies
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    • no.79
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    • pp.5-32
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    • 2024
  • The Korean government ranked 3rd among 193 UN member countries in the UN's 2022 e-Government Development Index. Korea, which has consistently been evaluated as a top country, can clearly be said to be a leading country in the world of e-government. The lubricant of e-government is data. Data itself is neither information nor a record, but it is a source of information and records and a resource of knowledge. Since administrative actions through electronic systems have become widespread, the production and technology of data-based records have naturally expanded and evolved. Technology may seem value-neutral, but in fact, technology itself reflects a specific worldview. The digital order of new technologies, armed with hyper-connectivity and super-intelligence, not only has a profound influence on traditional power structures, but also has an a similar influence on existing information and knowledge transmission media. Moreover, new technologies and media, including data-based generative artificial intelligence, are by far the hot topic. It can be seen that the all-round growth and spread of digital technology has led to the augmentation of human capabilities and the outsourcing of thinking. This also involves a variety of problems, ranging from deep fakes and other fake images, auto profiling, AI lies hallucination that creates them as if they were real, and copyright infringement of machine learning data. Moreover, radical connectivity capabilities enable the instantaneous sharing of vast amounts of data and rely on the technological unconscious to generate actions without awareness. Another irony of the digital world and online network, which is based on immaterial distribution and logical existence, is that access and contact can only be made through physical tools. Digital information is a logical object, but digital resources cannot be read or utilized without some type of device to relay it. In that respect, machines in today's technological society have gone beyond the level of simple assistance, and there are points at which it is difficult to say that the entry of machines into human society is a natural change pattern due to advanced technological development. This is because perspectives on machines will change over time. Important is the social and cultural implications of changes in the way records are produced as a result of communication and actions through machines. Even in the archive field, what problems will a data-based archive society face due to technological changes toward a hyper-intelligence and hyper-connected society, and who will prove the continuous activity of records and data and what will be the main drivers of media change? It is time to research whether this will happen. This study began with the need to recognize that archives are not only records that are the result of actions, but also data as strategic assets. Through this, author considered how to expand traditional boundaries and achieves reterritorialization in a data-driven society.

Egg Development and Morphology of Larva and Juvenile of Liparis tanakae in the Coastal Waters off Yeosu (여수 연안산 꼼치(Liparis tanakae)의 난발생 및 자치어 형태발달)

  • Kyung-Ae Jung;Na-Young Jeon;Sang-Hun Cha;Sung-Hoon Lee;Tae-Sik Yu;Keong-Ho Han
    • Korean Journal of Ichthyology
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    • v.35 no.4
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    • pp.263-269
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    • 2023
  • This study aims to contribute to the research on resource recovery for the rapidly declining population of Liparis tanakae by observing the larval development process and the morphology of juveniles based on their growth. Natural spawning eggs collected in Yeosu were used for observing the process of egg development and larval morphology. The water temperature during the rearing process was maintained at 12.3~13.5℃ (average 12.7℃). The fertilized eggs had an egg diameter ranging from 1.57 to 1.79 mm (average 1.71 mm) and were spherical and adhesive. Within 4 hours 35 minutes after fertilization, they reached the two-cell stage, and after 74 hours 10 minutes, the formation of the yolk sac began. At 106 hours post-fertilization, a caudal fin appeared at the tail tip. Hatching began at 526 hours, and the larvae developed with the yolk sac positioned just behind the eyes. The newly hatched larvae had both the mouth and anus open. Melanophores appeared inside the lower jaw and around the tail on the third day after hatching. By the 16th day after hatching, most of the yolk was absorbed, and melanophores were visible in the head region. Finally, on the 63rd day after hatching, the head region significantly developed, and the body shape and mouth were similar to those of an adult fish, signifying the transition to the juvenile stage. This study will serve as valuable data for aquaculture techniques related to the conservation and restoration of fish species based on the hatching and juvenile morphology of Liparis tanakae.

Physical Properties of Surface Sediments of the KR(Korea Reserved) 1, 2, and 5 Areas, Northeastern Equatorial Pacific (북동태평양 대한민국 광구 KR1, 2, 5 지역 표층 퇴적물의 물리적 특성 비교)

  • Lee, Hyun-Bok;Chi, Sang-Bum;Park, Cheong-Kee;Kim, Ki-Hyune;Ju, Se-Jong;Oh, Jae-Kyung
    • The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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    • v.13 no.3
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    • pp.168-177
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    • 2008
  • Trafficablility of a miner and potential environmental impacts due to mining activities should be considered in the selection of a commercial manganese nodule mining site. These two factors can be evaluated comparatively with physical properties and shear strength of sea-bed sediments. For the qualitative comparison of potential minig sites in terms of these two factors, physical properties such as water contents, void ratios, porosities, and grain densities, and shear strengths of surface sediments were determined for the three potential manganese nodule mining sites(KR1, KR2, and KR5) in the Korean manganese nodule contract area, northeast Pacific. For the study, sediment samples were collected from 107 stations from 2004 to 2006. The physical properties of surface sediments showed more significant differences between northern(KR1, KR2) and southern(KR5) blocks than between northern blocks(KR1 vs. KR2). Water content, void ratio, and porosity of sediments from KR5 were relatively higher than those from KR1 and KR2. Grain density of sediments from KR5 was relatively lower than those from KR1 and KR2. Shear strengths of the top 10cm sediments were higher in KR1 and KR2, whereas those of the deeper part were highest in KR5 block. Generally, sediments of high water contents are less suspendible than those of the low water contents by benthic disturbances, thus less disturbance is expected in the sediments of high water content by mining activities. In terms of trafficability, the shear strength of sediment below 10 cm deep is more important than shallower part because miner will disturb at least top 10 cm interval of the surface sediments. Base on these results, we conclude that KR5 area will be the best site for commercial mining among three investigated sites in this study.

Distribution and Remineralization Ratio of Inorganic Nutrients in the Divergence Zone($7^{\circ}{\sim}10.5^{\circ}N$), Northeastern Pacific (북동태평양 발산대 해역($7^{\circ}{\sim}10.5^{\circ}N$)의 무기영양염 분포와 재무기질화 비율)

  • Son, Ju-Won;Kim, Kyeong-Hong;Kim, Mi-Jin;Son, Seung-Kyu;Chi, Sang-Bum;Hwang, Keun-Choon;Park, Yong-Chul
    • The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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    • v.13 no.3
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    • pp.178-189
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    • 2008
  • The distribution of inorganic nutrients and their remineralization ratio in the divergence zone ($7^{\circ}{\sim}10.5^{\circ}N$) of the northeastern Pacific were investigated from July 2003 to July 2007. A divergence zone along the boundary of the North Equatorial Counter Current (NECC) and North Equatorial Current (NEC) at $10^{\circ}N$ was observed in July 2007 when the La Nina event and divergence-related upwelling was strong. The mean depth of oligotrophic surface mixed layer in the divergence zone was 46, 61, and 30 m in July 2003, August 2005, and July 2007, respectively. Below the surface mixed layer, a nutricline was clearly observed. The depth integrated value of nitrate including nitrite (DIVn) in the upper layer($0{\sim}100$ m depth) ranged from 5.51 to 21.71 $gN/m^2$(mean 12.82 $gN/m^2$) in July 2003, from 5.62 to 8.46 $gN/m^2$ (mean 7.15 $gN/m^2$) in August 2005, and from 8.98 to 27.80 $gN/m^2$(mean 21.12 $gN/m^2$) in July 2007. The maximum DIVn was observed at the divergence zone. The distributions of phosphate(DIVp) and silicate(DIVsi) were similar to that of DIVn and the DIVn/DIVsi ratio was $0.87{\pm}0.11$ in the upper layer. The limiting nutrient for phytoplankton growth in the study area was identified as nitrogen(N/P ratio=14.6). The nitrate (including nitrite) concentrations were lower in the region mainly affected by NEC than in the region affected by NECC. The study area of low silicate concentrations was also considered to be Si-limiting environment. The remineralization ratios of nutrients were $P/N/-O_2=1/14.6{\pm}1.1/100.4{\pm}8.8(23.44{\leq}Sigma-{\theta}{\leq}26.38)$ in the study area. These ratios suggested remineralization process in the surface layer of divergence zone.

Korean Sentence Generation Using Phoneme-Level LSTM Language Model (한국어 음소 단위 LSTM 언어모델을 이용한 문장 생성)

  • Ahn, SungMahn;Chung, Yeojin;Lee, Jaejoon;Yang, Jiheon
    • Journal of Intelligence and Information Systems
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    • v.23 no.2
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    • pp.71-88
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    • 2017
  • Language models were originally developed for speech recognition and language processing. Using a set of example sentences, a language model predicts the next word or character based on sequential input data. N-gram models have been widely used but this model cannot model the correlation between the input units efficiently since it is a probabilistic model which are based on the frequency of each unit in the training set. Recently, as the deep learning algorithm has been developed, a recurrent neural network (RNN) model and a long short-term memory (LSTM) model have been widely used for the neural language model (Ahn, 2016; Kim et al., 2016; Lee et al., 2016). These models can reflect dependency between the objects that are entered sequentially into the model (Gers and Schmidhuber, 2001; Mikolov et al., 2010; Sundermeyer et al., 2012). In order to learning the neural language model, texts need to be decomposed into words or morphemes. Since, however, a training set of sentences includes a huge number of words or morphemes in general, the size of dictionary is very large and so it increases model complexity. In addition, word-level or morpheme-level models are able to generate vocabularies only which are contained in the training set. Furthermore, with highly morphological languages such as Turkish, Hungarian, Russian, Finnish or Korean, morpheme analyzers have more chance to cause errors in decomposition process (Lankinen et al., 2016). Therefore, this paper proposes a phoneme-level language model for Korean language based on LSTM models. A phoneme such as a vowel or a consonant is the smallest unit that comprises Korean texts. We construct the language model using three or four LSTM layers. Each model was trained using Stochastic Gradient Algorithm and more advanced optimization algorithms such as Adagrad, RMSprop, Adadelta, Adam, Adamax, and Nadam. Simulation study was done with Old Testament texts using a deep learning package Keras based the Theano. After pre-processing the texts, the dataset included 74 of unique characters including vowels, consonants, and punctuation marks. Then we constructed an input vector with 20 consecutive characters and an output with a following 21st character. Finally, total 1,023,411 sets of input-output vectors were included in the dataset and we divided them into training, validation, testsets with proportion 70:15:15. All the simulation were conducted on a system equipped with an Intel Xeon CPU (16 cores) and a NVIDIA GeForce GTX 1080 GPU. We compared the loss function evaluated for the validation set, the perplexity evaluated for the test set, and the time to be taken for training each model. As a result, all the optimization algorithms but the stochastic gradient algorithm showed similar validation loss and perplexity, which are clearly superior to those of the stochastic gradient algorithm. The stochastic gradient algorithm took the longest time to be trained for both 3- and 4-LSTM models. On average, the 4-LSTM layer model took 69% longer training time than the 3-LSTM layer model. However, the validation loss and perplexity were not improved significantly or became even worse for specific conditions. On the other hand, when comparing the automatically generated sentences, the 4-LSTM layer model tended to generate the sentences which are closer to the natural language than the 3-LSTM model. Although there were slight differences in the completeness of the generated sentences between the models, the sentence generation performance was quite satisfactory in any simulation conditions: they generated only legitimate Korean letters and the use of postposition and the conjugation of verbs were almost perfect in the sense of grammar. The results of this study are expected to be widely used for the processing of Korean language in the field of language processing and speech recognition, which are the basis of artificial intelligence systems.

Studies on Soil Conservation Effects of the Straw-mat Mulchings (I) - Vegetation Establishment and Erosion Control Effects - (볏짚거적덮기공의 사방효과(砂防効果)에 관(關)한 연구(硏究)(I) - 사면지피조성(斜面地被造成) 및 침식방지(浸蝕防止) 효과(効果) -)

  • Woo, Bo Myong
    • Journal of Korean Society of Forest Science
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    • v.13 no.1
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    • pp.67-78
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    • 1971
  • The measures of contour-terracing with sod has been executed as a major measures for hillside erosion control works for a long time in Korea. It is, however, recognized that pair terracings make a new slope-face having the more steeper degree of slope between the upper and the lower terraces on hillsides and it also does not contribute for establishing the natural vegetation-cover by penetration of pioneer seeds on the slope faces or cut-faces of hillsides. The study was therefore conducted in connection with the above problems on the cut-face having slope of $40^{\circ}$ and 1.6 meter in slope length with clay soils. Plot allocation for the experiment consists of 3 kinds of 3 replica plots having each $1.6m^2$ of slope area, i. e., the control plot with direct seeding on slopes only ($T_1$), the covering plot with the straw-mats after seeding on slopes ($T_2$) and the seeding plot after covering with the straw-mats. ($T_3$). The main results obtained may be summarized as follows : 1. Effects of the straw-mat mulchings on surface soil loss control:-The total amount of soil losses from each treatments are measured as 4,651 gr from $T_1$, 163 gr. from $T_2$ and 2,891 gr. from $T_3$ treatment respectively. (Refer to table No. 2, 3 and 4). In short, it is recognized that effect of $T_2$ treatment is compared as 28.5 times than that of $T_1$ treatment and 17.7 times than that of $T_3$ treatment respectively. Effect of $T_3$ treatment compared with $T_1$ treatment is also such recognizable as 1.6 times in control of surface soil losses on a slope face. 2. Effect of the straw-mat mulchings on soil moisture content on slopes; -Average per cent of surface soil moisture content by treatments show as 21.60 at the $T_1$, 23.04 at the $T_2$ and 22.21 at the $T_3$ treatment respectively and that of subsurface soil moisture content by treatment show as 23.81 at the $T_1$, 26.16 at the $T_2$ and 24.81 at the $T_3$ treatment respectively. The variance of soil moisture content by treatments was highly significant (Refer table No. 7, 8 and 9). 3. Effect of the straw-mat mulchings on vegetation establishment;-Average numbers of germination by treatments are counted as 237 Nos. at the $T_1$, 246 Nos. at the $T_2$ and 262 Nos. at the $T_3$ treatment plots and the vegetation coverage on ground was almost same as about 90% of covers in all treatments. This effect is more or less lower than that of surface soil erosion control. 4. Regarding the effect on surface soil erosion control, the straw-mat mulchings would be effective as a new measures for control of soil erosion on erosion susceptible lands such slope-faced bare-lands as cut-fill faces, mass-movement faces and bare hillsides.

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Effect of Domestic Clay Minerals on Growth Performance and Carcass Characteristics in Growing-Fattening Hanwoo Steers (육성비육 거세한우에 대한 점토광물 급여가 성장 및 도체특성에 미치는 영향)

  • Kang, S.W.;Kim, J.S.;Cho, W.M.;Ahn, B.S.;Ki, G.S.;Son, Y.S.
    • Journal of Animal Science and Technology
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    • v.44 no.3
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    • pp.327-340
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    • 2002
  • This study was conducted to investigate the effects of domestic clay minerals on feed efficiency, meat quantity, meat quality and economic traits in 24 head of Hanwoo steers(166.1kg in body weight) for 540 days from six to 24 months in age. Feeding trial was conducted with 4 treatment(six heads/treatment) which were T1(Control), T2(Control+Kaolinite), T3(Control+Bentonite), T4(Control+Illite). The results obtained are summarized as follows; The range of average daily gains were 0.682 to 0.713, 0.669 to 0.714, 0.690 to 0.840 and 0.699 to 0.756kg in growing, fattening, finishing and over-all period, respectively, and the gains were high in T1 for growing and fattening period but in clay mineral groups for finishing and over-all period, especially it was high in Illite and Bentonite groups. Concentrates and TDN intakes per unit of kg gains were lower in clay mineral groups than in control and was lower especially in Bentonite groups. In carcass characteristics, dressed carcass and fresh meat and retailed cut percent were not apparently difference by treatments, and yield index was 69.3, 68.9, 68.8 and 68.6 in T3, T2, T4 and T1, respectively. Marbling scores were 5.1, 4.6, 4.4 and 3.3 in T3, T2, T4 and T1, respectively, and the range of shear force by treatment was from 3.51 to 6.02kg/cm2. and were improved with significant difference(P<0.05) in clay mineral groups than in control. Also in palatability traits, panel test scores of juiciness, tenderness and flavor were improved in clay mineral feeding groups, especially the flavor was improved with highly significant difference(P<0.01) in clay mineral groups than in control. In total fatty acid contents, the rate of SFA(saturated fatty acid) in longissimus muscle of beef was higher in the order of T2, T3, T1 and T4 while the rate of MUFA(monounsaturated fatty acid) was high in the order of T4, T3, T1 and T2. The content of oleic acid which is major influential factor at the flavor of beef was higher in Illite groups than in any other groups. In composition of amino acids in longissimus muscles of beef, the rate of essential amino acids was high in the order of T1, T2, T3 and T4. and the rate of amino acids in clay mineral groups was smaller than in control.In chemical component in Gom-Tang(soup of bone) made by Hanwoo steer’s leg-bone, the ranges of crude protein, ether extract, and crude ash was 0.81 to 1.24, 0.17 to 0.35 and 0.07 to 0.09%, respectively. In mineral composition, the ranges of Ca, P, Na and Mg was 14.01 to 15.77, 11.45 to 16.40, 37.92 to 49.99 and 0.26 to 0.46ppm, respectively. Chemical composition were not apparently different but mineral composition was increased in clay mineral groups than in control. Income by treatments was 967,096 to 1,524,055 Won per head for 540 days and income of clay mineral groups in comparison with control’s increased by 23.7 to 57.6 percent, and especially it was higher in bentonite and(or) Illite groups than others. According to the above results it may be concluded that clay mineral to growing-fattening Hanwoo steers can be improved the meat quantity, meat quality and income. Especially the effect of bentonite and illite is large and can be recommended for usage to improve animal performance as feed additives of growing-fattening Hanwoo steers.

Herbicidal Phytotoxicity under Adverse Environments and Countermeasures (불량환경하(不良環境下)에서의 제초제(除草劑) 약해(藥害)와 경감기술(輕減技術))

  • Kwon, Y.W.;Hwang, H.S.;Kang, B.H.
    • Korean Journal of Weed Science
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    • v.13 no.4
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    • pp.210-233
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    • 1993
  • The herbicide has become indispensable as much as nitrogen fertilizer in Korean agriculture from 1970 onwards. It is estimated that in 1991 more than 40 herbicides were registered for rice crop and treated to an area 1.41 times the rice acreage ; more than 30 herbicides were registered for field crops and treated to 89% of the crop area ; the treatment acreage of 3 non-selective foliar-applied herbicides reached 2,555 thousand hectares. During the last 25 years herbicides have benefited the Korean farmers substantially in labor, cost and time of farming. Any herbicide which causes crop injury in ordinary uses is not allowed to register in most country. Herbicides, however, can cause crop injury more or less when they are misused, abused or used under adverse environments. The herbicide use more than 100% of crop acreage means an increased probability of which herbicides are used wrong or under adverse situation. This is true as evidenced by that about 25% of farmers have experienced the herbicide caused crop injury more than once during last 10 years on authors' nationwide surveys in 1992 and 1993 ; one-half of the injury incidences were with crop yield loss greater than 10%. Crop injury caused by herbicide had not occurred to a serious extent in the 1960s when the herbicides fewer than 5 were used by farmers to the field less than 12% of total acreage. Farmers ascribed about 53% of the herbicidal injury incidences at their fields to their misuses such as overdose, careless or improper application, off-time application or wrong choice of the herbicide, etc. While 47% of the incidences were mainly due to adverse natural conditions. Such misuses can be reduced to a minimum through enhanced education/extension services for right uses and, although undesirable, increased farmers' experiences of phytotoxicity. The most difficult primary problem arises from lack of countermeasures for farmers to cope with various adverse environmental conditions. At present almost all the herbicides have"Do not use!" instructions on label to avoid crop injury under adverse environments. These "Do not use!" situations Include sandy, highly percolating, or infertile soils, cool water gushing paddy, poorly draining paddy, terraced paddy, too wet or dry soils, days of abnormally cool or high air temperature, etc. Meanwhile, the cultivated lands are under poor conditions : the average organic matter content ranges 2.5 to 2.8% in paddy soil and 2.0 to 2.6% in upland soil ; the canon exchange capacity ranges 8 to 12 m.e. ; approximately 43% of paddy and 56% of upland are of sandy to sandy gravel soil ; only 42% of paddy and 16% of upland fields are on flat land. The present situation would mean that about 40 to 50% of soil applied herbicides are used on the field where the label instructs "Do not use!". Yet no positive effort has been made for 25 years long by government or companies to develop countermeasures. It is a really sophisticated social problem. In the 1960s and 1970s a subside program to incoporate hillside red clayish soil into sandy paddy as well as campaign for increased application of compost to the field had been operating. Yet majority of the sandy soils remains sandy and the program and campaign had been stopped. With regard to this sandy soil problem the authors have developed a method of "split application of a herbicide onto sandy soil field". A model case study has been carried out with success and is introduced with key procedure in this paper. Climate is variable in its nature. Among the climatic components sudden fall or rise in temperature is hardly avoidable for a crop plant. Our spring air temperature fluctuates so much ; for example, the daily mean air temperature of Inchon city varied from 6.31 to $16.81^{\circ}C$ on April 20, early seeding time of crops, within${\times}$2Sd range of 30 year records. Seeding early in season means an increased liability to phytotoxicity, and this will be more evident in direct water-seeding of rice. About 20% of farmers depend on the cold underground-water pumped for rice irrigation. If the well is deep over 70m, the fresh water may be about $10^{\circ}C$ cold. The water should be warmed to about $20^{\circ}C$ before irrigation. This is not so practiced well by farmers. In addition to the forementioned adverse conditions there exist many other aspects to be amended. Among them the worst for liquid spray type herbicides is almost total lacking in proper knowledge of nozzle types and concern with even spray by the administrative, rural extension officers, company and farmers. Even not available in the market are the nozzles and sprayers appropriate for herbicides spray. Most people perceive all the pesticide sprayers same and concern much with the speed and easiness of spray, not with correct spray. There exist many points to be improved to minimize herbicidal phytotoxicity in Korea and many ways to achieve the goal. First of all it is suggested that 1) the present evaluation of a new herbicide at standard and double doses in registration trials is to be an evaluation for standard, double and triple doses to exploit the response slope in making decision for approval and recommendation of different dose for different situation on label, 2) the government is to recognize the facts and nature of the present problem to correct the present misperceptions and to develop an appropriate national program for improvement of soil conditions, spray equipment, extention manpower and services, 3) the researchers are to enhance researches on the countermeasures and 4) the herbicide makers/dealers are to correct their misperceptions and policy for sales, to develop database on the detailed use conditions of consumer one by one and to serve the consumers with direct counsel based on the database.

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