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Anti-Aspergillus Activities of the Ligusticum chuanxiong Essential Oil Alone and in Combination with Antibiotics

  • Sim, Youn;Shin, Seung-Won
    • Natural Product Sciences
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    • v.16 no.3
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    • pp.175-179
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    • 2010
  • The present study aimed to assess the antifungal properties of the essential oil fraction from Ligusticum chuanxiong (Umbelliferae) and its components against five clinically important Aspergillus species. The essential oil fraction was extracted from the underground parts of the plant by steam distillation, and its main components, namely, Z-ligustilide, butylidene phthalide, and p-cresol were isolated by column chromatography. The antifungal activities of the essential oils were evaluated by the broth dilution method. Both the total essential oil fraction of L. chuanxiong and its components showed significant anti-Aspergillus activity against all five tested strains with MICs between 62.5 and 250 ${\mu}g$/ml, respectively. In a checkerboard microtiter assay, the combination of antibiotics, itraconazole with the essential oil fraction of L. chuanxiong or its main components exhibited synergistic or additive, and in some cases indifferent, effects against the tested Aspergillus species, resulting in FICIs (fractional inhibiting concentration indices) ranging from 0.12 to 2, while the combination of antibiotics, amphothericin B with L. chuanxiong essential oils mostly showed antagonistic effects.

A Case Study of the Appropriate Measure of Herbei Spirit Oil Pollution Damages on the Hanging and Floating Netcage Aquaculture Fisheries (허베이스피리트호 유류오염피해배상에서 수하식 및 가두리식 양식어업의 피해정도사정 사례)

  • Kang, Young-Joo;Kim, Ki-Soo
    • The Journal of Fisheries Business Administration
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    • v.44 no.1
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    • pp.15-24
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    • 2013
  • Recently the Korean court accepts two typies of fisheries damages caused by oil pollution.. One is the direct loss of fisheries production originated from pollution of oil spillover. The other is the indirect loss of fisheries production originated from governmental measure of restricting fishing activities because of safety of marine product of oil spillover areas. The paper tries to suggest the appropriated measure of oil pollution damages of hanging and floating netcage aquaculture fisheries using the court judgement for responsibility restriction on Herbei Spirit Case. The paper tries to compare the damge estimation method of floating netcage aquaculture fisheries with that of natural aquaculture fisheries using conventional theory of the population biology of living resources characterized with age distribution.

Performance of environment friendly insulating dielectric oil for power transformer (친환경 변압기 절연유의 특성)

  • Han, Dong-Hee;Cho, Han-Goo;Han, Se-Won;Ahn, Myung-Sang
    • Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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    • 2004.07a
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    • pp.453-456
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    • 2004
  • This paper surveys the latest findings on vegetable-oil-based dielectric coolants in power systems. In recent years, environmental concerns have been increased on the use of poorly biodegradable mineral oils in distribution and power transformers in regions where spills from leaks and equipment failure could contaminate the surroundings. In addition, there are demands to improve equipment efficiencies in power systems. In this reason, researches were started in the mid 1990s to develop a fully biodegradable dielectric coolants. Vegetable oil was considered the most likely candidate for a fully biodegradable dielectric coolants. Vegetable-oil-based dielectric coolants provide the advantages of high level of biodegradability, renewable natural resource, non-toxic properties, enhanced fire safety, more effective cooling and good dielectric strength for many electrical equipment.

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Trends of Green Policies of Biogas Renewable Technology using POME in Malaysia (말레이시아 팜오일폐수 POME(Palm Oil Mill Effluent)를 이용한 바이오가스 신재생에너지기술 그린정책 동향)

  • Park, Young Gyu
    • Journal of Korea Society of Waste Management
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    • v.35 no.7
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    • pp.571-586
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    • 2018
  • The Malaysian biogas upgrading technologies and policies were examined. In Malaysia, the regulation of palm oil mill effluent (POME) has been enforced to reduce the biochemical oxygen demand to 20 ppm and the biogas capture in the palm oil mills have been recently enforced for renewable energy. A huge amount of organic waste is produced from POME, and 80 million tons from palm oil trees, every year. Due to the renewable energy trends, the Malaysian government is modifying the use of biogases as fuels in favor of their conversion into compressed natural gas (CNG) and other chemicals; various green policies are being promoted because of many advantages of the organic substances. The Korean policies for biogas are a good model for exporting environmental plants after upgrading the digestion and purification technologies. Therefore, this article introduces the current status of POME and biogas production in Malaysia, it could encourage creating a new market for biomethane.

In situ isolation and characterization of the biosurfactants of B. Subtilis

  • Akthar, Wasim S.;Aadham, Mohamed Sheik;Nisha, Arif S.
    • Advances in environmental research
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    • v.9 no.3
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    • pp.215-232
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    • 2020
  • Crude oils are essential source of energy. It is majorly found in geographical locations beneath the earth's surface and crude oil is the main factor for the economic developments in the world. Natural crude oil contains unrefined petroleum composed of hydrocarbons of various molecular weights and it contains other organic materials like aromatic compounds, sulphur compounds, and many other organic compounds. These hydrocarbons are rapidly getting degraded by biosurfactant producing microorganisms. The present study deals with the isolation, purification, and characterization of biosurfactant producing microorganism from oil-contaminated soil. The ability of the microorganism producing biosurfactant was investigated by well diffusion method, drop collapse test, emulsification test, oil displacement activity, and blue agar plate method. The isolate obtained from the oil contaminated soil was identified as Bacillus subtilis. The identification was done by microscopic examinations and further characterization was done by Biochemical tests and 16SrRNA gene sequencing. Purification of the biosurfactant was performed by simple liquid-liquid extraction, and characterization of extracted biosurfactants was done using Fourier transform infrared spectroscopy (FTIR). The degradation of crude oil upon treatment with the partially purified biosurfactant was analyzed by FTIR spectroscopy and Gas-chromatography mass spectroscopy (GC-MS).

Determination of Protein and Oil Contents in Soybean Seed by Near Infrared Reflectance Spectroscopy

  • Choung, Myoung-Gun;Baek, In-Youl;Kang, Sung-Taeg;Han, Won-Young;Shin, Doo-Chull;Moon, Huhn-Pal;Kang, Kwang-Hee
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.46 no.2
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    • pp.106-111
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    • 2001
  • The applicability of near infrared reflectance spectroscopy(NIRS) was tested to determine the protein and oil contents in ground soybean [Glycine max (L.) Merr.] seeds. A total of 189 soybean calibration samples and 103 validation samples were used for NIRS equation development and validation, respectively. In the NIRS equation of protein, the most accurate equation was obtained at 2, 8, 6, 1(2nd derivative, 8 nm gap, 6 points smoothing and 1 point second smoothing) math treatment condition with SNV-D (Standard Normal Variate and Detrend) scatter correction method and entire spectrum by using MPLS (Modified Partial Least Squares) regression. In the case of oil, the best equation was obtained at 1, 4, 4, 1 condition with SNV-D scatter correction method and near infrared (1100-2500nm) region by using MPLS regression. Validation of these NIRS equations showed very low bias (protein:-0.016%, oil : -0.011 %) and standard error of prediction (SEP, protein: 0.437%, oil: 0.377%) and very high coefficient of determination ($R^2$, protein: 0.985, oil : 0.965). Therefore, these NIRS equation seems reliable for determining the protein and oil content, and NIRS method could be used as a mass screening method of soybean seed.

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Extension of Shelf Life of Kimchi by Addition of Encapsulated Mustard Oil

  • Kim, Mi-Jeong;Ha, Jee-Yun;Yun, Ye-Rang;Noh, Jeong-Sook;Song, Yeong-Bok;Song, Yeong-Ok
    • Food Science and Biotechnology
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    • v.15 no.6
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    • pp.884-888
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    • 2006
  • In this study, we have attempted to characterize the effects of encapsulated mustard oil with regard to the extension of the shelf life of kimchi. The quantity of mustard oil or encapsulated mustard oil added to the brined cabbage in this study was 0.05%(w/w). Overall, the fermentation processes in the encapsulated mustard oil-added kimchi (EMO) and mustard oil-added kimchi (MO) occurred at a slower rate than in the controls. The periods during which the MO and EMO kimchi samples were edible were prolonged for more than two weeks, according to measurements of pH, total acidity, and microbial changes. The overall acceptability of EMO was superior to MO throughout the entirety of the fermentation period. Sensory evaluation verified that EMO yielded a more favorable product than the MO and control varieties. Encapsulated mustard oil can be employed as a natural food additive to prolong the shelf life of kimchi, via an induced delay of the fermentation process.

Chemical Compositions and Insecticidal Activity of Eucalyptus urophylla Essential oil Against Culex quinquefasciatus Mosquito

  • Pujiarti, Rini;Kasmudjo, Kasmudjo
    • Journal of the Korean Wood Science and Technology
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    • v.44 no.4
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    • pp.494-504
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    • 2016
  • Eucalyptus oils are widely used as spices, perfume industrial materials, food flavorings, and medicines. Several types of Eucalyptus oils also have insecticidal activity and as carminative. This study investigated the chemical composition, insecticidal (larvicidal and repellent) activity of E. urophylla oil against filarial mosquito Culex quinquefasciatus. E. urophylla oil was obtained from fresh leaves by water-steam distillation with oil yield 1.08%. E. urophylla oil in this study had no color (clear), has odor (typical eucalyptus), with specific gravity 0.941; refractive index 1.465; miscibility in 70% ethanol 1 : 3; and optical rotation (-) $5.83^{\circ}$. The major compounds of the oil were ${\alpha}$-pinene (11.73%), 1,8-cineole (49.86%), ${\beta}$-ocimene (6.25%), ${\gamma}$-terpinene (9.11%), and ${\alpha}$-terpinyl acetate (7.63%). The result showed the excellent insecticide activity against C. quinquefasciatus. The oil provided larvicidal activity with $LC_{50}$: 80.21 ppm and $LC_{90}$: 210.18 ppm, and repellent activity with $IC_{50}$: 0.82% and $IC_{90}$: 4.88%. The present study showed the effectiveness of E. urophylla as natural insecticide against C. quinquefasciatus, the mosquito vector of filariasis.

Chemical Composition of Seed in Medicinal Soybean Collected in Korea

  • Seong, Rak-Chun;Kim, Jeong-Gyu;Hwang, Young-Hyun
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.43 no.3
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    • pp.141-146
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    • 1998
  • Production of medicinal soybean [Glycine max (L.) Merrill], characterized with black seed, white stripe at hilum border, yellow cotyledon and small seed, is increasing with increasing consumption. The objective of this study was to investigate the chemical composition of medicinal soybean seed and to provide basic information, for the characterization of these soybeans among genetic resources. Forty-four lines of medicinal soybeans collected from Korea and two control cultivars, 'Hwangkeumkong' (Yellow seed coat) and 'Geomjeongkong l' (Black seed coat) were planted at the Research Farm of the College of Natural Resources, Korea University, located at Namyangju City on May 25, 1996. Seeds of these lines were harvested at full maturity and analyzed for protein, oil, sugar, starch and mineral contents. Mean protein and oil content of the medicinal line seeds were 42.6 and 16.1%, respectively, and those of the control cultivars were in the middle range for protein and oil content. However, sugar and starch content of the medicinal line seeds appeared to be in the lower range of the distributions compared to the control cultivars and were 10.0 and 1.68%, respectively. Mean P, K, Ca, and Mg contents of the seeds of medicinal soybean lines were 15.9, 21.5, 3.11, and 2.81 mg/g, respectively, indicating that these lines had higher P, K, and Mg and lower Ca contents when compared to the control cultivars. Mean Na and Fe contents were 671 and 224 mg/kg, respectively, showing lower Na and similar Fe contents. The observed results provided that chemical compositions of medicinal soybean were, on average, different from those of the general soybean cultivars.

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Effect of immune-enhancing enteral nutrition formula enriched with plant-derived n-3 fatty acids on natural killer cell activity in rehabilitation patients

  • Cho, Jung Min;Choi, Hyo Seon;Cho, Youn Soo;Park, So Young;Kim, Deog Young;Lee, Jong Ho
    • Nutrition Research and Practice
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    • v.13 no.5
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    • pp.384-392
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    • 2019
  • BACKGROUND/OBJECTIVES: Enteral nutrition formulas with immune-enhancing nutrients, such as n-3 fatty acids, may manage patients' nutritional status and pathophysiological processes. The aim of our study was to investigate natural killer (NK) cell activity alterations and related cytokine changes resulting from feeding with soybean oil-containing enteral nutrition formula (control group) and plant-derived n-3 fatty acid-enriched enteral nutrition formula. SUBJECTS/METHODS: Subjects participated for 14 consecutive days and consumed enteral formula containing canola and flaxseed oil (n3EN, test group) in nonsurgical patients hospitalized for rehabilitation. Blood samples were collected on the first day and 14 days after the consumption of each formula daily, and anthropometric parameters were collected. Hematology and biochemical values were analyzed, and NK cell activities and serum cytokine concentration were measured. A total of sixty subjects were included in the analysis, excluding dropouts. RESULTS: No significant differences were found in biochemical parameters. The n3EN group's NK cell activities at effector:tumor cell ratios of 10:1, 5:1, 2.5:1 and 0.625:1 were significantly higher than those of the control group after two weeks (P < 0.05). However, there were no statistically significant differences in serum cytokine interleukin (IL)-12, $interferon-{\gamma}$, $IL-1{\beta}$, IL-6 and tumor necrosis $factor-{\alpha}$ values between the two groups. CONCLUSIONS: In conclusion, this study elucidates the beneficial effects of plant-derived n-3 fatty acid supplementation in enteral formula on NK cell activity.