• 한국미생물·생명공학회지
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• 제24권4호
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• pp.472-477
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• 1996

Natural human epidermal growth factor (nhEGF) was purified from pregnant human urine by benzoic acid adsorption, DEAE-Sepharose ion exchange, and immunoaffinity chromatography. The purified nhEGF was further separated into four fractions using Bondapak C$_{18}$ HPLC system. Following characterization by Western blot analysis and double immu- nodiffusion, we found that each fraction corresponds to four derivatives of the nhEGF. For biological analysis of nhEGF, we optimized the labeling time and serum concentration for the incorporatioin of 5-bromo-2'-deoxy uridine (BrdU), a non-radioactive alternative for [$^{3}$H]-thymidine uptake, into NIH 3T3 cells. The DNA synthesis of NIH 3T3 cells was gradually increased at the nhEGF concentrations between 0.1 - 10 ng/ml in the Dulbecco's Modified Eagles Medium (DMEM) containing 0.2% Fetal calf serum (FCS). When we assayed the biological activity of four fractions, the activity of the second fraction was superior to that of the others. Based on the results from the HPLC analysis spiked with recombinant human epidermal growth factor (rhEGF) and amino acid sequencing, we concluded that the second fraction was nhEGF and the other three fractions were the derivatives of nhEGF. In addition, the proportion of nhEGF was approximately 46% is compared with that of the other three derivatives.

• Archives of Pharmacal Research
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• 제27권6호
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• pp.615-618
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• 2004

In the course of screening for reactive oxygen species scavengers from natural products, an antioxidant was isolated from the mycelial culture broth of Phellinus linteus and identified as hispidin. The hispidin content was reached its maximum level at 12 days after onset of inoculation. About 2.5 mg／mL of hispidin was produced by P linteus in a yeast-malt medium (pH 5.8, $25^{\circ}C$). Hispidin inhibited 22.6 and 56.8% of the super oxide anion radical, 79.4 and 95.3% of the hydroxyl radical, and 28.1 and 85.5% of the DPPH radical at 0.1 and 1.0 mM, respectively. The positive control ${\alpha}-tocopherol$ scavenged 25.6 and 60.3%, 74.6 and 96.3%, and 32.7 and 77.5% of each radical, respectively, at the same concentrations. However, hispidin showed no significant activity on the hydrogen peroxide radical.

• 한국주거학회논문집
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• 제20권6호
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• pp.11-18
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• 2009

The apartments of South Korea has developed enormously from 1960's to the present. Nevertheless, People have not paid much attention to the exterior colors used in the apartments. For this reason, this study tries to understand the differences of the colors between each brands and areas based on the newly-built apartments in Jeonju and Gunsan, and this focuses on the colors used in the main buildings, public facilities, playgrounds, and the main entrances. According to the research, although six-cases all showed that YR was higher than any other color patterns, but there were differences from both the brands and the areas. Both the colors and the materials, as distinguished by the brands, were more diverse in the apartments by the small or medium businesses than in those by the big businesses. Moreover, when the identical brands were observed in Jeonju and Gunsan, the differences between the areas was more recognizable than the differences between the brands. Both the colors and the materials, as distinguished by the areas, had more varieties in Gunsan than in Jeonju. It was because Gunsan had many diversities in natural, historical or social backgrounds, as compared to Jeonju. Unlike other studies, this study researched public facilities as well as the main buildings so that it was possible to perceive the harmony of the colors in the apartment complex; however, the cases used in this study were limited numerically.

• Structural Engineering and Mechanics
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• 제33권6호
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• pp.725-745
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• 2009

In this paper, probabilistic seismic performance assessment of a typical non-seismic RC frame building representative of a large inventory of existing buildings in developing countries is conducted. Nonlinear time-history analyses of the sample building are performed with 20 large-magnitude medium distance ground motions scaled to different levels of intensity represented by peak ground acceleration and 5% damped elastic spectral acceleration at the first mode period of the building. The hysteretic model used in the analyses accommodates stiffness degradation, ductility-based strength decay, hysteretic energy-based strength decay and pinching due to gap opening and closing. The maximum inter story drift ratios obtained from the time-history analyses are plotted against the ground motion intensities. A method is defined for obtaining the yielding and collapse capacity of the analyzed structure using these curves. The fragility curves for yielding and collapse damage levels are developed by statistically interpreting the results of the time-history analyses. Hazard-survival curves are generated by changing the horizontal axis of the fragility curves from ground motion intensities to their annual probability of exceedance using the log-log linear ground motion hazard model. The results express at a glance the probabilities of yielding and collapse against various levels of ground motion intensities.

• 한국의학물리학회지:의학물리
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• 제7권1호
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• pp.25-35
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• 1996

혼탁한 물질인 생체조직에서 신진대사, 혈액확산, 혈중산소의 비침습적 측정에 관한 연구를 광을 이용한 방법으로 제시하였다. 생체조직내의 산소포화도와 혈액분량을 측정하기 위하여 660nm와 880nm 의 광파장을 이용하여 측정하였으며, 생체조직에서 얻은 광 세기의 데이터는 조직내의 깊은 곳에서 생리적인 변화를 나타낸다. 데이터의 평가는 혈액분량과 산소포화도의 변화에 대한 지수의 기울기로서 조사하여 평가하였다. 여기서 곡선으로 나타낸 지수는 각각의 파장에서 기준파장의 세기와 측정부위에서의 반사광의 세기를 비율에 의한 자연대수로 나타냈다. 장단지 근육에 대한 실험 결과에 따르면 산소의 지수는 운동중에 현저하게 변화를 보여 주었다.

• Bulletin of the Korean Chemical Society
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• 제34권8호
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• pp.2487-2490
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• 2013

SA51, a medium potency inhibitor of protein tyrosine phosphatase 1B (PTP1B), was identified to be a potent inhibitor of $I{\kappa}B$ kinase ${\beta}$ ($IKK{\beta}$). Consistent with this, SA51 prevented lipopolysaccharide (LPS)-induced breakdown of $I{\kappa}B{\alpha}$ in macrophages. The effects of SA51 in mice were compared with those of structurally related compounds, SA18 and SA32, which were previously reported as inhibitors of both enzymes - less potent against $IKK{\beta}$ but more potent against PTP1B compared to SA51. SA51 improved glucose tolerance and lipid parameters in mice, consistent with the results reported for $IKK{\beta}^{+/-}$ mice. In contrast, SA18 and SA32 showed anti-obesity effects without anti-diabetic effects. Collectively, the effects of SA51 could be due largely to the inhibition of $IKK{\beta}$, whereas SA18 and SA32 may be more likely to inhibit PTP1B, consistent with their relative in vitro inhibitory effects.

• Structural Engineering and Mechanics
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• 제54권6호
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• pp.1061-1073
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• 2015

This paper presents a nonlocal sinusoidal shear deformation beam theory (SDBT) for the nonlinear vibration of single walled carbon nanotubes (CNTs). The present model is capable of capturing both small scale effect and transverse shear deformation effects of CNTs, and does not require shear correction factors. The surrounding elastic medium is simulated based on Pasternak foundation. Based on the nonlocal differential constitutive relations of Eringen, the equations of motion of the CNTs are derived using Hamilton's principle. Differential quadrature method (DQM) for the natural frequency is presented for different boundary conditions, and the obtained results are compared with those predicted by the nonlocal Timoshenko beam theory (TBT). The effects of nonlocal parameter, boundary condition, aspect ratio on the frequency of CNTs are considered. The comparison firmly establishes that the present beam theory can accurately predict the vibration responses of CNTs.

• 한국동물학회:학술대회논문집
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• 한국동물학회 1997년도 제18회 생물과학 심포지움 강연록
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• pp.42-57
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• 1997

The multicriterion matrix technique (MM-technique) was proposed for a method of monitoring and assessment about vegetation naturalness. Four criteria and 10 subcriteria were selected and two evaluation indices such as VN-value and VN-class were used. The criteria were characterized by syntaxonomical informations of hemeroby concept and potential natural vegetation, hierarchical system between criteria, and ordinal scale of VN-values. VN-values were classified into 11 ordinal levels and condensed to five VN-classes for facilitating practical use. A vegetation map of naturalness described by combination o( two indices was proposed as an alternative resolution of the DGN map. We also discuss the organization of the map content which is a matter of grid size (unit-area). In the case study, a grid size proper to show a full account of real information of actual vegetation is less 250-grid (250 $\times$ 250 $m^2$) in a medium size of city area containing relatively fragmented ecosystems. In conclusion, it was recognized that this new assessment technique was useful and vegetation assessment was accomplished with the smaller grid size in Korea.

• 약학회지
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• 제49권1호
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• pp.68-73
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• 2005

Epi-xanthatin analogs containing hydrophilic substituents such as carboxylic acid, alcohol, morpholine, amino acid, and glucose derivatives were synthesized and their in vitro cytotoxicity and in vivo antitumor activity were evaluated. The target compounds were generally cytotoxic against tumor cell lines of human origin with $ED_{50}$ values of $0.1{\sim}30{\mu}g/ml$, except the highly hydrophilic analog 6 containing aspartic acid. Contrary to the potent cytotoxicity weakly hydrophilic analogs 2 and 8 were not active in vivo, or even toxic to the test animals. As a result, hydrophilic analog of epi-xanthatin did not show in vitro cytotoxicity and hydrophobic analogs did not show in vivo antitumor activity, thus it is presumed that amphiphilic analogs or those with medium hydrophilicity would exhibit the antitumor potency in vivo.

• 한국수소및신에너지학회논문집
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• 제26권5호
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• pp.469-476
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• 2015

The purpose of this work is to investigate main factors to design a solid-state hydrogen stroage system with magnesium hydride with 10 wt% graphite using numerical simulation tools. The heat transfer characteristic of this material was measured in order to perform the highly reliable simulation for this system. Based on the measured effective thermal conductivity, a transient heat and mass transfer simulation revealed that the total performance of hydrogen storage system is prone to depend on heat and mass transfer behaviors of hydrogen storage medium instead of its inherent kinetic rate for hydrogen adsorption. Furthermore, we demonstrate that the thermodynamic aspect between equlibrium presssure and temperature is one of key factor to design the hydrogen storage system with high performance using magnesium hydride.