• Title/Summary/Keyword: natural insecticide

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Protein structure analysis : Pharmacophore study for new insecticide target AnCE using the substrate of ACE, HHL molecule (단백질의 구조연구 : ACE의 기질 HHL을 이용한 신규 살충제 표적 AnCE에 대한 약리단 연구)

  • Lee, Jung-Kyung;Kim, Kyeong-Yee
    • The Korean Journal of Pesticide Science
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    • v.9 no.3
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    • pp.191-198
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    • 2005
  • Hippuryl-L-histidyl-L-leucine (Hip-L-His-L-Leu, HHL) is the known substrate of ACE, which used often in inhibition kinetic study to design new inhibitor. Here we use HHL molecule as a template to predict pharmacophore which can interact with residues in active site of AnCE, new potential insecticide target protein. To explain physicochemical properties related to molecular geometry and conformational change in reaction field as well as electron density of atoms associated to pharmacophores, geometry optimization, NMR chemical shifts and natural population analysis were performed by ab initio and DFT method. Calculated NMR chemical shifts showed good agreement with the experimental ones and obtained electron densities were used for analyzing pharmacophores of corresponding atoms. Finally, we could extract aye pharmacophores related to hydrophobic aliphatic and aromatic site, hydrogen bonding donor and acceptor site and Zn binding site from the HHL molecule.

An Integrated Approach in the Pest Management in Sericulture

  • Singh, R.N.;Saratchandra, Beera
    • International Journal of Industrial Entomology and Biomaterials
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    • v.5 no.2
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    • pp.141-151
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    • 2002
  • The success of sericulture industry in India is mainly attributed to the well-planned annual sericultural activity and the systematic implementation of pest preventive and control measures. The insect spectrum of silkworm and its food plants is complex and plays a major role in limiting the production of silk. Insects cause extensive damage to plant whereas predators and parasites either kill the silkworm larvae or force them to spin flimsy cocoons. Unilateral control measure against this pest is mainly based on the use of synthetic organic insecticides. Though these approaches initially paid rich dividends, the undesirable consequences soon surfaced. Insecticide induced resurgence of gall midges, leafhopper, leaf roller, secondary pest out breaks and development of pest biotypes has led to realization of Integrated Pest Management in sericulture. Various components of IPM, viz. Host plant resistance, cultural practices, biological control, chemical control and integrating them at various technological levels have been studied. Sources of host plant resistance have been identified for some of the major insect pests. High yielding mulberry variety has been propagated and their resistances towards major pests have been recorded. Cultural practices like pruning, pollarding, judicious use of nitrogen, optimum spacing and weed management have preyed to be the powerful tools in containing pests. Natural control over the pest population build- up exerted by the wide range of parasitoids, predators and pathogens has been well documented with identification of natural enemies and studies on their potential. Augmentation, through inoculation or inundative releases of parasitic arthropods, is the most direct way of increasing the numbers of these beneficials in sericulture.

The Public Perception and Attitude on the Medical Insects and Pest Control in Korea

  • Lyu, Eun-Soon;Kang, Hye-Sook;Lee, Dong-Kyu
    • Korean Journal of Health Education and Promotion
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    • v.1 no.1
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    • pp.1-13
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    • 1999
  • A study has been performed on perception of citizens about the medical insects (house flies, mosquitoes, cockroaches) in Chang Won city. A total of 375 subjects (male: 180, female: 195) was surveyed in October 1997. Cockroaches were the most dislike of medical insects to the dwellers. At a residence, cockroaches were the most troubled insects although mosquitoes were the most frequently appeared insects. Also, the most troubled insects were the cockroaches at hospitals, departments, coffee shops, and bars; the mosquitoes at theaters and parks; and the house flies at restaurants in the city. The cockroaches had the highest negative opinion score as a 4.4 point of a 5.0 point. About half of the subjects had extremely suffered from the mosquitoes (52.8%) and cockroaches (51.8%). House residents controlled house flies using fly swatters (50.0%) and insecticide sprayers (38.9%), but apartment residents controlled them using the sprayers (50.8%) and fly swatters (36.5%). The mosquitoes were controlled by using the sprayers (51.6%) and mosquito coils (36.4%). The cockroaches were controlled by using toxic baits (32.9%) and the sprayers (31.5%). Only 10% of the subjects were satisfied control management against the medical insects. The subjects wanted to more effectively control against cockroaches (39.9%) and mosquitoes (37.9%).

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Natural parasitism of parasitoids on Phyllocnistis citrella (Lepidoptera: Gracillaridae) in Jeju citrus orchards in Korea (제주 감귤원에서 귤굴나방에 대한 포식 기생자 천적의 자연 기생율)

  • Hyun, Seung Young;Lim, Un Taek;Kim, Dong-Soon
    • Korean journal of applied entomology
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    • v.58 no.4
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    • pp.299-303
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    • 2019
  • This study was conducted to investigate the species of native parasitoids attacking Phyllocnistis citrella and their parasitism in citrus orchards in Jeju, Korea. During the survey period from 2013 to 2014, seven parasitoids belonging to Chalcidoidea were found: majority of the parasitoids were Sympiesis striatipes with 73% and Quadrastichus sp. with 22% in Eulophidae. The others were Neochrysocharis sp., Pnigalio sp., Holcopelte sp., Ageniaspis sp. (Encyrtidae) and Trichomalopsis sp. (Pteromalidae). Average parasitism rate reached 10.7% in orchards with spraying insecticide, and 32.2% in environmental-friendly managed orchards.

A Report on Mixed Occurrence of Tobacco Whitefly (Bemisia tabaci) Biotypes B and Q in Oriental Melon Farms in Kyungpook Province, Korea (담배가루이 생태형 B와 Q가 같이 발생하는 경북 참외재배 지역 보고)

  • Kim, Eunsung;Kim, Yonggyun
    • Korean journal of applied entomology
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    • v.53 no.4
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    • pp.465-472
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    • 2014
  • The tobacco whitefly, Bemisia tabaci, infest the Oriental melon and give significant economic damage along with its virus-vectoring activity. Various biotypes of B. tabaci have been well known and are classified depending on the severity of crop damage and insecticide susceptibility. In this study, B. tabaci adults were collected in the melon fields located in Poongchun-myeon, Andong, Korea and diagnosed on their biotypes using PCR molecular markers. From the all the 11 greenhouses, B. tabaci biotype Q was identified. In addition, biotype B adults were also found from the 4 greenhouses. These results report the first occurrence of B. tabaci at the Oriental melon farms in Gyeongbuk province with mixed infection by the two biotypes in the area.

Insecticidal Activities of Aromatic Plant Extracts against Four Agricultural Insects

  • Kim, Soon-Il;Shin, Ook-Kyun;Song, Cheol;Cho, Kwang-Yun;Ahn, Young-Joon
    • Journal of Applied Biological Chemistry
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    • v.44 no.1
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    • pp.23-26
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    • 2001
  • The insecticidal activities of 30 aromatic plant extracts against four insect pests were examined by direct contact application. Against Nilaparvata lugens female adults, Myzus persicae female adults, and Plutella xylostella larvae, at 5,000 ppm, >90% mortality was achieved in the methanol extracts of the rhizomes from Cnidium officinale, Acorus gramineus, Acorus calamus var. angustatus and Curcuma Ionga, the whole plant from Agastache rugosa, the fruits from Illicium verum and Piper nigrum, and the flower bud from Eugenia caryophyllata as well as Cinnamomum camphora steam distillate. Against Spodoptera litura larvae, all test plant extracts were ineffective. The plants described merit further study as potential insect-control agents against insect pests.

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Copying and Manipulating Nature: Innovation for Textile Materials

  • Rossbach, Volker;Patanathabutr, Pajaera;Wichitwechkarn, Jesdawan
    • Fibers and Polymers
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    • v.4 no.1
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    • pp.8-14
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    • 2003
  • This paper considers the potential impact of biological approaches such as bio-copying (biomimetics) and biomanipulating (e.g. genetic engineering) on future developments in the field of textiles and, in particular, fibres. If analytical tools for studying biological systems combined with those of materials science are further developed, and higher efficiency and reproducibility of genetic engineering technology can be achieved, the potential for the copying and manipulation of nature for textile innovations will be immense. The present state for both fields is described with examples such as touch and close fastener, structurally coloured fibres, the Lotus of lect (for bio-copying), as well as herbicide tolerant cotton, insecticide resistant cotton (Bt cotton), cotton polyester bicomponent fibres, genetically engineered silkworm and silk protein, and spider fibres. (for genetic engineering).

Mosquito Control Using Entomopathogenic Fungi (곤충병원성 곰팡이를 이용한 모기 방제)

  • Choi, Kwang Shik;Jung, Hee-Young
    • The Korean Journal of Mycology
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    • v.43 no.2
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    • pp.77-87
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    • 2015
  • Insects are commonly infected by fungal diseases and are mostly susceptible to them. Increasing levels of insecticide resistance has recently become an issue for control programs; thus, research has focused on mosquito control using entomopathogenic fungi, including fungal pathogens such as Beauveria bassiana, Metarhizium anisopliae, and Lagenidium giganteum. Review discusses entomopathogenic fungi related to control programs for mosquito transmitted vector-borne diseases such as dengue, filariasis, malaria, and yellow fever, and how to use entomopathogenic fungi for mosquito control.

Determination of Methoxyfenozide Residues in Water and Soil by Liquid Chromatography: Evaluation of its Environmental Fate Under Laboratory Conditions

  • Choi, Jeong-Heui;Mamun, M.I.R.;Shin, Eun-Ho;Kim, Hee-Kwon;El-Aty, A.M. Abd;Shim, Jae-Han
    • Toxicological Research
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    • v.24 no.3
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    • pp.207-212
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    • 2008
  • Pesticide residues play several key roles as environmental and food pollutants and it is crucial to develop a method for the rapid determination of pesticide residues in environments. In this study, a simple, effective, and sensitive method has been developed for the quantitative analysis of methoxyfenozide in water and soil when kept under laboratory conditions. The content of methoxyfenozide in water and soil was analyzed by first purifying the compound through liquid-liquid extraction and partitioning followed by florisil gel filtration. Upon the completion of the purification step the residual levels were monitored through high performance liquid chromatography(HPLC) using a UV absorbance detector. The average recoveries of methoxyfenozide from three replicates spiked at two different concentrations and were ranged from 83.5% to 110.3% and from 98.1% to 102.8% in water and soil, respectively. The limits of detection(LODs) and limits of quantitation(LOQs) were 0.004 vs. 0.012 ppm and 0.008 vs. 0.024 ppm, respectively. The method was successfully applied to evaluate the behavioral fate of a 21% wettable powder(WP) methoxyfenozide throughout the course of 14 days. A first-order model was found to accurately fit the dissipation of methoxyfenozide in water with and a $DT_{50}$ value of 3.03 days was calculated from the fit. This result indicates that methoxyfenozide dissipates rapidly and does not accumulate in water.

Inheritance of Insecticide Resistance to Plant- and Leaf-hoppers Inherited Properties of MEP Resistance to Small Brown Plant-hopper (Laodelphax striatellus Fallen) (멸구 매미충류에 대한 약제저항성의 유전성에 관한 연구 I. 애멸구의 MEP제에 대한 약제저항성의 유전적 특성)

  • Shim Jai Wook
    • Korean journal of applied entomology
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    • v.17 no.2 s.35
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    • pp.75-80
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    • 1978
  • The study was conducted to determine the inherited properties of the MEB resistance to natural population of the small brown plant-hopper(Laodelphax striatelluss). The plant-hoppers were collected from the natural population of Naju area where the highest insecticide resistance of MEF to the green rice leaf hopper (Nephotettix cincticeps) have been examined in 1976. And Naju collections were crossed to the susceptible Lab stock to examine the MEP resistance in the $F_1,\;BC_1F_1\;and\;F_2$ and $F_2$ populations. Ail the data were analyzed by the probit method. There was a difference in MEP resistance between Naju collection and susceptible Lab stock, showing $LD_{50}$ value of the former was 0.0029ug/insect compared to 0.0008ug/insect for the later. The $LD_{50}$ values and dosage-mortality lines of the $F_1$ and $BC_1F_1$ tended to close their resistant parent. and it was considered that the character of the MEP resistance in the Naju collection of the small brown plant hopper was controlled by the genetic traits. However, $LD_{50}$ value and dosasage-mortality lines of the $F_2$ populations were intermediate to their parents, it would be conclusive that the trait will be governed by a interaction of the genes or factors rather than the single genic control.

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