• Title/Summary/Keyword: natural colorants

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Structure and Function of the Genes Involved in the Biosynthesis of Carotenoids in the Mucorales

  • Iturriaga, Enrique A.;Velayos, Antonio;Eslava, Arturo P.
    • Biotechnology and Bioprocess Engineering:BBE
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    • v.5 no.4
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    • pp.263-274
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    • 2000
  • Carotenoids are widely distributed natural pigments which are in an increasing demand by the market, due to their applicatins in the human food, animal feed, cosmetics, and pharmaceutical industries. Although more than 600 carotenoids have been identified in nature, only a few are industrially important (${\beta}$-carotene, astaxanthin, lutein or lycopene). To date chemical processes manufacture most of the carotenoid production, but the interest for carotenoids of biological origin is growing since theire is an increased public concern over the safety of artificial food colorants. Although much interest and effort has been devoted to the use of biological sources for industrially important carotenoids, only the production of biological ${\beta}$-carotene and astaxanthin has been reported. Among fungi, several Mucorales strains, particularly Blakeslea trispora, have been used to develop fermentation processes for the production of ${\beta}$-carotene on almost competitive cost-price levels. Similarly, the basidiomycetous yeast Xanthophyllomyces dendrorhous (the perfect state of Phaffia rhodozyma), has been proposed as a promising source of astaxanthin. This paper focuses on recent findings on the fungal pathways for carotenoid production, especially the structure and function of the genes involved in the biosynthesis of carotenoids in the Mucorales. An outlook of the possibilities of an increased industrial production of carotenoids, based on metabolic engineering of fungi for carotenoid content and composition, is also discussed.

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The Effect of Chitosan Treatment of Fabrics on the Natural Dyeing using Loess (키토산 처리포의 황토염색에 관한 연구)

  • Kwon, Min-Soo;Jeon, Dong-Won;Kim, Jong-Jun
    • Fashion & Textile Research Journal
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    • v.7 no.3
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    • pp.327-332
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    • 2005
  • The purpose of this study is to investigate the effect of chitosan treatment on the dyeing of cotton fabric specimens using loess as colorants. The wet pick up ratio of the chitosan acid solution, as well as the drying condition after the padding of the fabric specimens, was changed in order to study the loess uptake on the fabric. The average particle diameter of the loess was measured. Main components of the loess were $SiO_2$, $Al_2O_3$, and $Fe_2O_3$. By the chitosan treatment, the loess amount on the cotton fabric increased. 80% wet pick up ratio of the chitosan acid solution on the cotton fabric specimen allowed more stable and even adhesion of the loess on the fabric surface, compared to the cases of 100% and 120% wet pick up ratio.

A Study on the improving Dyeing properties of Natural Dyes (천연염료의 염색성 향상에 관한 연구)

  • 서명희
    • Proceedings of the Korea Contents Association Conference
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    • 2004.05a
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    • pp.473-477
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    • 2004
  • Cotton fabric was treated with Cationon UK(tertiary ammonium salt) to improve the dyeability to black tea colorants. In order to investigate the efficacy of Cationon UK for improving dyeing properties of cotton fabric, the effect of Cationon UK treatment conditions and dyeing conditions on dye uptake of the cotton fabrics treated with Cationon UK were examined and the colorfastness to washing, perspiration, rubbing and light was also evaluated for practical uses. Cationized cotton treated with Cationon UK showed high dye uptake at lower dyeing concentration and shorter dyeing time, compared with untreated. Dyeing temperature did not affect significantly dye uptake and the maximum dye uptake was obtained at pH 5. Most colorfastness was relatively good, showing 4/5∼5 rating.

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Study on Genotoxicity of Crocin, a Component of Gardenia Fruit, in Bacterial and Mammalian Cell Systems

  • Choi, Hae-Yeon;Kim, Youn-Jung;Jeon, Hee-Kyung;Ryu, Jae-Chun
    • Molecular & Cellular Toxicology
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    • v.4 no.4
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    • pp.285-292
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    • 2008
  • Crocin is one of the major components of gardenia fruit and saffron which are widely used as natural food colorants and as traditional Chinese medicines. However, the genotoxicity data on crocin are not sufficient for safety evaluation. The purpose of this study was the examination of the genotoxicity on crocin from gardenia yellow in bacterial and mammalian cells, using various genotoxic battery testing assays and the influence of crocin on methyl methanesulfonate (MMS) and ${H_2}{O_2}$-induced DNA damage in vitro, using single cell gel electrophoresis (comet) assay. From results, no considerable mutagenicity and clastogenicity were seen in bacteria and mammalian cells treated with crocin, by Ames test, chromosomal aberration assay, ${tk}^{+/-}$ gene forward mutation assay and comet assay. And, post-treatment with crocin significantly suppressed ${H_2}{O_2}$-induced DNA damage in a dose-dependent manner. In conclusion, the findings of the present study and other previous observations indicate that crocin has no genotoxic potential. And it showed that crocin clearly repressed the genotoxic potency of ${H_2}{O_2}$. These results suggest that anti-oxidative effects of crocin may be involved in the protective effects of DNA damage.

Dyeing with Natural Dye (II) ―Dyeing of Silk with Sappan Wood― (천연염료에 의한 염색(II) ―소목에 의한 견염색―)

  • Nam, Sung Woo;Chung, In Mo;Kim, In Hoi
    • Textile Coloration and Finishing
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    • v.7 no.4
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    • pp.87-96
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    • 1995
  • Colorant concentrates are prepared by use of concentration method of colorants extracted from Sappan wood using met hanol. Dyeabilities and fastness properties of silk fabrics dyed with concentrate of Sappan wood are investigated.The results obtained are as follows; 1. The storage stability of colorant concentrate is poor as concentration is excess. 2. Because the colors of dyeings are different with mordants, aluminium acetate, copper acetate, chromium alum and tin chloride are used in order to obtain reddish color. 3. The optimum dyeing temperature and dyeing time are 6$0^{\circ}C$ and 60 min, respectively. 4. Dry cleaning fastness is very good, and light fastness and washing fastness are low. 5. The dyeing method is simple owing to usehess of colorant concentrate prepared without extraction of colorant every time.

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BLEACHING EFFECT AND SURFACE CHANGE INDUCED BY 3 BLEACHING AGENTS ON THE EXTRINSICALLY STAINED TEETH (표백제에 따른 외인성 착색치아의 표백효과 및 표면변화에 관한 연구)

  • Lee, Jae-Yoon;Shin, Dong-Hoon;Hong, Chan-Ui
    • Restorative Dentistry and Endodontics
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    • v.20 no.2
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    • pp.802-811
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    • 1995
  • The purpose of this in vitro study was to compare the bleaching effect and the surface change according to three bleaching agents (Rembrandt, Natural White, Hi-Lite). Teeth were stained by artificial food colorants for 6 weeks and bleached for 4 weeks. The teeth were evaluated with spectrophotometer after bleaching on the 1st week and on the 4th week. The surface change of enamel was evaluated under SEM after exposure to 3 agents for 1 and 24 hours. The results were as follows : 1. All groups showed increased Lightness difference after bleaching. 2. After 1 week, only Rembrandt group showed significant bleaching effect compared to control group. 3. After 4 weeks, Rembrandt group and Hi-Lite group showed significant bleaching effect compared to control group. 4. SEM evaluation revealed that enamel surface underwent considerable change after 24 hours. Scratch disappeared and smooth surface was shown.

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Antioxidative Activity of Acylated Anthocyanin Isolated from Fruit and Vegetables

  • Park, Sang-Won;Chang, Eun-Ju;Ha, Tae-Youl;Park, Kyoung-Ho
    • Preventive Nutrition and Food Science
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    • v.2 no.3
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    • pp.191-196
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    • 1997
  • The antioxidative activity of seven different acylated anthocyanin pigments isolated from grape, sweet potato, eggplant and red cabbage was evaluated bvy using linoleic acid autoxidation and rat liver microsomal systems. The acylated anthocyanins were isolated and purified by Amberlite SAD-7, ODS and Sephadex LH-20 column omatography, and preparative HPLC. Most of acylated anthocyanisns exhibited antioxidative activity as strong as $\alpha$-tocoperol, and especially peonidin3-O--(6-O-trans-caffeyl)-2-O-(6-O-trans-feuloylglucopyranosyl)-$\beta$-D-glucopyranosyl-5-O-$\beta$-D-glucopyranoside from purple sweet potato showed the strongest activity, comparable to BHA (not significant, p<0.05) in the linoleic acid system. Meanwhile, two acylated anthocyanins from the pericarps of grape and eggplant inhibited considerably the MDA formation from rat liver microsomal lipid peroxidation induced by FeSO$_4$/$H_2O$$_2$. In particular, malvidin 3-O-(6-O-p-coumaroyl)-$\beta$-D-glucopyranosde from grape pericarps showed the strongest antioxidant activity, comparable to $\alpha$-tocopherol (not significant, p<0.05). These results suggest that the acylated anthocyanins from fruits and vegetables can be used as potential dietary ntioxidants and natural colorants.

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Natural Dyeing of Chitosan Crossinked Cotton Fabrics - Gromwell - (키토산 가교 처리된 면직물의 천연염색에 관한 연구 - 자초를 중심으로 -)

  • Kwak, Mi-Jung;Lee, Shin-Hee
    • Fashion & Textile Research Journal
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    • v.14 no.2
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    • pp.311-319
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    • 2012
  • The purpose of this study is to investigate the dyeing property of gromwell on modified cotton fabric by chitosan. Modified cotton fabrics were manufactured by crosslinking agent epichlorohydrin in the presence of chitosan. Gromwell colorants were extracted with methanol. Modified cotton fabrics dyed using gromwell were post-mordanted using Al, Fe and Cu. The dyeability (K/S) and color factors (L, a, b, ${\Delta}E$ and h) of modified cotton fabrics were measured by computer color matching. Additionally the fastness to washing and light were also investigated. The dye-uptake of modified cotton fabrics increased with the dyeing time. The saturated dyeing time was about 10minutes at $50^{\circ}C$. The dyeability (K/S) was remarkably increased with increasing content of chitosan because of having a amine group of chitosan. Modified cotton fabrics were dyed yellowish red by non and Fe mordanting, blueish red by Al and Cu mordanting, respectively. The washing fastness of non, Al, Fe and Cu mordant in the presence and absence of chitosan were increased $1{\rightarrow}2$, $3{\rightarrow}4$, $4{\rightarrow}4-5$ and $4{\rightarrow}4-5$ respectively. And light fastness of non, Al, Fe and Cu mordant in the presence and absence of chitosan were increased $1{\rightarrow}1-2$, $1{\rightarrow}1-2$, $1.2{\rightarrow}2.3$ and $1-2{\rightarrow}2$ respectively.

Bicomponent Finishing of Cotton Fabrics(II) - Chitosan and Charcoal - (면직물의 복합가공(II) - 키토산과 숯 -)

  • Bae, Ki-Hyun;Lee, Shin-Hee
    • Fashion & Textile Research Journal
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    • v.10 no.5
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    • pp.748-755
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    • 2008
  • The purpose of this study is to investigate the effect of chitosan treatment on the dyeing of cotton fabric using charcoal as colorants. Particle size of charcoal, dyeability(K/S), SEM morphology of dyed fabrics and color fastness were also investigated. In this study, cotton fabrics were treated with a crosslinking agent epichlorohydrin in the presence of chitosan to provide the cotton fabrics the dyeing properties of natural dye by the chemical linking of chitosan to the cellulose structure. The results obtained were as follows; Mean average diameter of charcoal was 1.44 ${\mu}m$. According to various conditions, the dyeing effects of 1% chitosan treatment on the dyeing of cotton fabrics using charcoal were the highest with 10%(owb) of charcoal at $90^{\circ}C$ for 120minutes and non-treatment of cotton fabrics were the highest with 15%(owb) of charcoal at $90^{\circ}C$ for 150minutes. Overall, K/S value of 1% chitosan treatment of cotton fabrics on the natural dyeing using charcoal was higher than non-treatment of cotton fabrics. It was observed the surfaces of cotton fabrics treated with chitosan were adsorbed with charcoal powders of particle size more than a non-treated chitosan fabric by SEM. The cotton fabrics were dyed with blackish gray color by charcoal gradually according to treating chitosan. The K/S value, that is indicative of dye affinity, became higher as the increase of treated chitosan concentration. The color fastness of charcoal, washing and light fastness was excellent as 4-5 grade.

Natural dyeing of Soybean Protein Fabrics - Gallnut - (대두섬유의 천연염색에 관한 연구 - 오배자를 중심으로 -)

  • Noh, Young-Ju;Lee, Shin-Hee
    • Fashion & Textile Research Journal
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    • v.16 no.3
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    • pp.462-468
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    • 2014
  • This study investigates the dyeability of soybean protein fabric after dyeing with gallut. In this study, the colorants of gallnut were extracted with boiling water at $60^{\circ}C$ and 60min. Soybean protein fabric was dyed with extracted solution from gallnut according to concentration, temperature and time. After dyeing with gallut solution, dyeability (K/S) was obtained by CCM observation. Al, Cu and Fe were employed as a mordanting agents. Various color changing were produced by the interactions of mordants that were different from soybean protein fabric; however, Al mordant was not indicated as a variable color change. Fastness to washing and light were also investigated. Consequently, the dyeability of the soybean protein fabric dyed with gallut showed reasonable results at 100% o.w.f, $90^{\circ}C$, for 60min. Fastness to washing according to concentration indicated good result as more than grade (4-5) in general. Fastness to light was almost the same compared to fastness to washing at all concentrations. Fastness to washing according to mordants indicated good grade (4) in principle at Al, Cu, and Fe; in addition, fastness to light indicated a good grade too. Fastness to light also indicated the highest dyeability at Fe mordant. This study shows that gallut is a reasonable dyestuff to soybean protein fabric and that it is possible to manufacture a variety of products that use soybean protein fabric.