• KSBB Journal
• /
• v.24 no.2
• /
• pp.170-176
• /
• 2009

Lectin was finally isolated on Sephadex G-100 from Korean soybean cultivars developed for soy source and investigated its some biochemical properties. Native PAGE pattern of this lectin revealed a molecular weight of 108 kDa as tetramer. The molecular weight of this lectin isolated as double protein band by SDS-PAGE was calculated to be 32 and 22 kDa from the relative mobilities compared with those of the standard proteins. Among the tested red blood cell, the isolated lectin agglutinated rabbit red blood cell treated with trypsin, but did not agglutinated human red blood cells (A, B, AB, O), rat, and untreated rabbit red blood cell. The optimal temperature and thermal stability of isolated lectin was at 20-$50^{\circ}C$ and 10-$60^{\circ}C$, respectively. This lectin was stable at 7.2, and showed complete loss in its activity below pH 6.2 and above pH 8.0.

• Journal of Microbiology and Biotechnology
• /
• v.18 no.2
• /
• pp.299-307
• /
• 2008

Soybean seed ferritin is essential for human iron supplementation and iron deficiency anemia prevention because it contains abundant bioavailable iron and is frequently consumed in the human diet. However, it is poorly understood in regards its several properties, such as iron mineralization, subunit assembly, and protein folding. To address these issues, we decided to prepare the soybean seed ferritin complex via a recombinant DNA approach. In this paper, we report a rapid and simple Escherichia coli expression system to produce the soybean seed ferritin complex. In this system, two subunits of soybean seed ferritin, H-2 and H-1, were encoded in a single plasmid, and optimal expression was achieved by additionally coexpressing a team of molecular chaperones, trigger factor and GroEL-GroES. The His-tagged ferritin complex was purified by $Ni^{2+}$ affinity chromatography, and an intact ferritin complex was obtained following His-tagged enterokinase (His-EK) digestion. The purified ferritin complex synthesized in E. coli demonstrated some reported features of its native counterpart from soybean seed, including an apparent molecular weight, multimeric assembly, and iron uptake activity. We believe that the strategy described in this paper may be of general utility in producing other recombinant plant ferritins built up from two types of subunits.

• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.2
• /
• pp.39-45
• /
• 1964

The phenotypic and genotypic correlations and heritabitities were calculated on 10 characters measured, using 10 native and 10 of introduced soybean varieties planted on 7 times with 15 days interval from April 15th. The genotypic correlations associated with grain yield were partitioned in to direct and indirect effects through the path coefficient analysis. The results are summarized as follows; 1. Generally high associations between characters were found, with high genotypic correlations than phenotypic, due to, probably, the fact that non segregating fixed varieties were used, and that the environmental effects were not elliminated appropreately here. Neverthless, the relative degree of association could be estimated. 2. The degree of associations of characters were different according the planting time, requiring the considerations on the specific characteristics depending on the specific planting time. 3. The introduced varieties were different with native ones in many respects, especially on the associations with stem length, stem diameter and seed weight. Here, the degree and the directions of the associations in the introduced ones were some peculier. 4. The less significant differences of characters between native varieties caused the heritability of native lower than that of introduced except the heritability of days from blooming to mature and seed weight. 5. The genetic associations of characters, measured, with grain yield were partitioned in to direct and indirect effects, calculating path coefficients. The days to bloom, stem length and stem diameter were most significantly affected the grain yield in the 5th planting of native ones.

• Journal of The Korean Society of Grassland and Forage Science
• /
• v.23 no.3
• /
• pp.143-150
• /
• 2003

This study was conducted to determine the response of main growth characters, yield and chemical composition of Jeju native soybean based on the difference between phosphate rates (0, 50, 100, 150, 200, 250kg/ha) from May 11 to September 10 in 2002 in Jeju island. Days to flowering was delayed from 92 days to 97 days as the increasing of phosphate rate. Also plant height grew longer from 109cm to 124cm. The number of branches and leaves, stem diameter, root length and weight of root grew powerful as phosphate rate increased from 0 to 250kg/ha. Fresh forage yield was 26.5MT/ha in the nonphosphate plot and then was 36.9MT/ha as phosphate rate increased to 250kg/ha. And the difference between 200kg/ha and 250kg/ha in phosphate rate was not significant. Dry matter, crude protein and TDN yield increased 6.0∼7.9MT/ha, 0.9∼1.4MT/ha and 3.4∼4.9MT/ha respectively, as the increasing of phosphate rate. Also crude protein, crude fat, NFE and TDN content increased 15.5∼18.3%, 2.2∼3.3%, 42.5∼43.5%, and 56.7∼61.9% respectively. In contrast with this, crude fiber and crude ash decreased 32.1∼28.1% and 7.7∼6.8% respectively. To reach the climax of forage yield was estimated optimum phosphate rate to be 200kg/ha.

• Journal of The Korean Society of Grassland and Forage Science
• /
• v.23 no.3
• /
• pp.135-142
• /
• 2003

Jeju native soybean was grown at five plant densities(30${\times}$30cm, 30${\times}$25cm, 30${\times}$20cm, 30${\times}$15cm, 30${\times}$10cm) from May 11 to September 10 in 2002 in Jeju island to determine the optimum plant density. Days to flowering was delayed from 94 days to 98 days as increasing of plant density. Plant height was 103cm at 30${\times}$30cm plot, as plant density increased, was 117cm at 30${\times}$10cm plot. As plant density increased, the number of branches and leaves, stem diameter, weight of plant, root length and weight of root grew low. As plant density increased from 30${\times}$30cm to 30${\times}$15cm, fresh forage, dry matter, crude protein and TDN yield increased 23.3∼36.5MT/ha, 5.1∼8.0MT/ha, 0.8∼1.4MT/ha and 2.9∼4.8MT/ha respectively, but decreased at 30${\times}$10cm plot. As plant density increased, crude protein, crude fat, NFE and TDN content increased 16.2∼17.9%, 2.7∼3.7%, 37.6∼40.7% and 56.1∼60.0% respectively. In contrast with this, crude fiber and crude ash decreased 34.9 ∼30.8% and 8.6∼7.2% respectively. To reach the climax of forage yield was estimated optimum plant density to be 30${\times}$15cm.

• Culinary science and hospitality research
• /
• v.13 no.4
• /
• pp.220-231
• /
• 2007

This study was designed to standardize the recipes of native local foods in Gijang region such as 'Miyeokseolchi', 'Molseolchi', 'Gijangumuk' and 'Seokbakji' and analyze their nutrients. The test recipe for each food was prepared according to the information obtained from the personal interview of Gijang natives and then applied to sensory evaluation. After that, CAN pro 3.0 was used for the nutritional evaluation. The results are as follows: Both Miyeokseolchi and Molseolchi were preferred with soybean sprouts added but Miyeokseolchi with fermented red pepper paste added. Gijangumuk was preferred with dissolved ceylon moss added, not filtered. Seokbakji was preferred with salt-fermented gizzard shad added. Nutrient analysis was performed according to the established standard recipe. In general, it appeared that energy content was low and amino acid contents such as glutamate, aspartic acid, leuicine and arginine were high. Major fatty acids in Miyeokseolchi and Molseolchi were a linoleic acid and Seokbakji has a palmitic acid and EPA.

• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.28 no.3
• /
• pp.345-350
• /
• 1983

Collected 38 varieties of colored-soybean seed cultivated under a given cultural conditions were tested in the various chemical compositions and rheological properties in seeds. As it were, protein, fat, carbohydrate, ash and water contents as a chemical contents were studied not only on their statistical variations, but also on interrelationships with their collaborated rheological characteristics; hardness, cohesiveness, elasticity, gumminess and chewiness. For the rheological analysis of the materials, the General Foods Texturometer and typical texturometer curve were used.

• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.25 no.3
• /
• pp.31-34
• /
• 1980

In order to evaluate the day length sensitivity of soybean lines, Korean native and other varieties were planted in different dates from May 4 to July 13. With early plantings flowering was delayed and little differences of flowering time among lines were observed with the early plantings as compared with those of late plantings. The positive relationship between number of days to flowering from plantings on May 4 and July 13 was found and screening for the day length sensitivity is recommended to plant either on the beginning of Mayor mid-July in the middle part of Korean peninsular. Among the soybean lines tested several day neutral lines were observed.

• BMB Reports
• /
• v.29 no.6
• /
• pp.540-544
• /
• 1996

Ferritins from germinated pumpkin seeds were isolated by ammonium sulfate precipitation (0.55 saturation), ion-exchange chromatography on DEAE-cellulose, and gel filtration chromatographies on Sephacryl S-300 and Sephadex G-100. Pumpkin ferritin contains less iron than soybean ferritin. Pumpkin ferritin cross-reacted with anti-soybean ferritin antiserum made in rabbit, and showed two distinct antibody reactive bands, both of equal intensity. The pumpkin ferritins corresponding to the two bands were separable by centrifugation in a sucrose gradient (20~50%). The molecular weights of the native pumpkin ferritins based on the estimation of sucrose gradient centrifugation, gel filtration on Sephacryl S-300 and non-denaturing polyacrylamide gel electrophoresis appeared to be: 530~580 KD (the large molecular weight pumpkin ferritin) and 330-360 KD (the small molecular weight pumpkin ferritin) The large molecular weight pumpkin ferritin contains less iron. Both pumpkin ferritins cross-reacted with anti-soybean ferritin antibody with a spur formation suggesting partial antigenic recognition.

• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.22 no.2
• /
• pp.93-97
• /
• 1977

The rate of damaged soybean seeds by pod borer averaged 7.4% with the range of 1.2%-21.3% and tended to be higher in the lines with hairy pods comparing to those with hairless pods. The susceptibility of pod borer was negatively correlated with the pod setting date. while was not significantly affected by the weather conditions during the pod setting period.