• Maxillofacial Plastic and Reconstructive Surgery
• /
• v.21 no.4
• /
• pp.332-344
• /
• 1999

Cytokines are hormone-like proteins which mediate and regulate inflammatory and immune responses. Interleukin-6 (IL-6) is involved in the final differentiation of B cells into antibody-producing cells. Interleukin-8 (IL-8) is a neutrophil chemotactic factor that plays an important role in the recruitment of neutrophil to inflammatory loci. Inflammatory mediators by cells in the gingiva have been implicated in the initiation and progression of periodontitis and oral infection. The purpose of this study was conducted to investigate the effect of lipopolysaccharide (LPS), staphylococcus enterotoxin B (SEB) on production of IL-6 and IL-8 by human gingival and facial dermal fibroblasts. Primary cultured human gingival and facial dermal fibroblasts were incubated with LPS (0.01, 0.1, $1.0{\mu}g/ml$), SEB (0.01, 0.1, $1.0{\mu}g/ml$) or LPS $(0.1{\mu}g/ml)$ plus SEB $(0.1{\mu}g/ml)$. Culture supernatants were collected at 24, 48, and 72 hrs and assessed for IL-6 and IL-8 production by enzyme-linked immunosorbent assay. IL-6 production in gingival fibroblasts stimulated with LPS was higher than that with SEB. IL-6 production by double exposure with LPS plus SEB was amplified in comparison with single exposure of LPS or SEB. IL-6 production in facial dermal fibroblasts was increased only by stimulation with a high concentration of LPS $(1.0{\mu}g/ml)$. Its production in facial dermal fibroblasts by exposure with SEB was decreased in comparison with control, nontreated cells. Therefore, gingival fibroblasts showed higher sensitivity than facial dermal fibroblasts in response to low concentration of LPS. Also, IL-6 production by double exposure with LPS plus SEB was amplified in comparison with single exposure of LPS or SEB. IL-8 production in gingival fibroblasts was enhanced greatly only by stimulation of high concentration of LPS $(1.0{\mu}g/ml)$. That by exposure with SEB was increased only in 24 hrs cultivation. IL-8 production by double exposure with LPS plus SEB was amplified in comparison with single exposure of LPS or SEB. IL-8 production in facial dermal fibroblasts was decreased by LPS and increased only in 48 hrs cultivation by SEB. IL-8 production by double exposure with LPS plus SEB was enhanced only in 48 hrs cultivation in comparison with single exposure of LPS or SEB. therefore, IL-6 and IL-8 production were released at various quantities according to bacterial toxin applied and site of fibroblast harvested. These results suggest that gingival fibroblasts may be concerned with IL-6 and IL-8 related inflammatory response more than facial dermal fibroblasts.

• Proceedings of the Korean Society of Crop Science Conference
• /
• 2017.06a
• /
• pp.326-326
• /
• 2017

Climate warming is the issue on the global scale. Soybean can be seriously damaged when high temperature occurs during a reproductive stage such as the flowering and pod-setting period according to the Representative Concentration Pathway (RCP) (2021~2100) 8.5 scenarios. The weather in 2016 was very different from other years (average for 30 years from 1980 to 2010) ; the highest temperature was $33.7^{\circ}C$ which was higher $3.29^{\circ}C$ than average temperature from last 30 years and average rainfall was 26.5 mm, lower 140.9 mm than average rainfalls from other years. Especially, the highest temperature during soybean flow-ering and pod setting stage was $26.8^{\circ}C$ which was higher $0.1^{\circ}C$ and rainfall was 172.2 mm, higher 47.8 mm than other years from the first to the 20th in the October at soybean seed elongation stage. Soybean leaves were turned upside down by the drought stress during the flowering and pod-setting stage. The numbe-r of pods and seeds per unit area decreased 11.0% and 30.3% compared with the previous year, respectively. The ripening period was prolonged by 21 days because of high temperature and soil moisture contents due to the continual rainmade increase of the seed weight up to 15.6% and the yield decreased 7.1% compared to the previous year.

• Proceedings of the Korean Society of Crop Science Conference
• /
• 2017.06a
• /
• pp.229-229
• /
• 2017

This research study was conducted to analyze the characteristics of different rice cultivars in abnormal temperature conditions (low temperature) for ripening period abnormalities, and to investigate the physiological causes behind the abnormalities. Four Korean high quality japonica-type rice cultivars, Jinbu (JB), Junamjosaeng (JJ), Geumyoung (GY), Hwawang (HW) were used in the experiment. The following day after flowering, they were then moved into two phytotrons under natural daylight with 65% RH but controlled at different temperatures - one at $19/29^{\circ}C$ (night/day) and the other at $13/23^{\circ}C$ as the low - temperature study on ripening. For the cultivars at $13/23^{\circ}C$ (low temperature study), JB and JJ had a ripening rate of 93% which is similar to the ripening rates of cultivars at $19/29^{\circ}C$ at 45 days after heading (DAH). In contrast, GY and HW recorded lower ripening rates of 86% and 57% respectively. However, when the cultivars at $13/23^{\circ}C$ were harvested at 61 DAH (when the accumulated temperature reached $1100^{\circ}C$), the difference in ripening rates compared to the 4 cultivars of $19/29^{\circ}C$ harvested at 45 DAH was not obvious (JB 94%, JJ 97%, GY 97%, HW 88%). Starch content showed little difference among the 4 cultivars at different temperature conditions while amylose content was higher for cultivars at $13/23^{\circ}C$ compared to those at $19/29^{\circ}C$. In addition, the enzyme activities of starch biosynthesis were about 5~10 days slower in cultivars at $13/23^{\circ}C$ compared to cultivars at $19/29^{\circ}C$. The grain-filling rate showed highly significant correlations with the enzyme activities of Sucrose synthase ($R^2=0.70^{***}$), ADP glucose pyrophosphorylase ($R^2=0.63^{***}$), UDP glucose pyrophosphorylase ($R^2=0.36^{***}$), Starch synthase ($R^2=0.51^{***}$), and Starch branching enzyme ($R^2=0.59^{***}$). Among the enzymes, Sucrose synthase activity had the highest correlation coefficient with grain-filling rate. In conclusion, the activity of enzymes such as Sucrose synthase, UDP glucose pyrophosphorylase, ADP glucose pyrophosphorylase, Starch synthase, Starch branching enzyme in starch biosynthesis is proven to be highly related to the grain filling process. Notably, the decrease in the activity of Sucrose synthase and Starch branching enzyme and the late increase in ADP glucose pyrophosphorylase activity at low temperature in the ripening stage are considered to be disadvantageous as they delay ripening and increased amylose content.

• Proceedings of the Korean Society of Crop Science Conference
• /
• 2017.06a
• /
• pp.317-317
• /
• 2017

We performed this experiment to select high quality rice adapted to wheat-rice double cropping system. We sowed barley and wheat seed in November 2, 2015. After harvesting barley and wheat, we transplanted high quality rice cultivars: 'Unkwang', 'Hopun', 'Haepum', 'Hyunoum' in June 17 and 24. We used 'sindongjin' rice as a control. As a result, 'Hyunpum' had a highest head rice yield regardless of transplanting date. Head rice yield of 'Hyunpum' was 488.1kg/10a when transplanted in June 17, and 453.6kg/10a when transplanted in June 24. The reason for this highest head rice yield was not percentage of head rice but milled rice yield. Milled rice yield of 'Hyunpum' was 526.5kg/10a when transplanted in June 17, and 490.0kg/10a when transplanted in June 24. Percentage of head rice had little differences between rice cultivars. Among yield components, only number of panicle per $m^2$ had an effect on the differences of yield between rice cultivars. The other yield components didn't have an effect on the differences of yield between rice cultivars. Compared to June 17, only 'Unkwang' had higher head rice percentage and head rice yield in June 24. Head rice percentage of 'Unkwang' changed from 67.3% to 85.0% and head rice yield changed from 324kg/10a to 393.8kg/10a when transplanting date delayed from June 17 to June 24. When transplanting date was delayed, heading date of 'Unkwang' was more delayed than other rice cultivars. By delay of heading date, mean temperature for 40days changed from $25.0^{\circ}C$ to $22.9^{\circ}C$ which improved temperature condition during grain filling stage. This improved head rice percentage and head rice yield of 'Unkwang' transplanted in June 24. If transplanting date is more delayed than June 24, 'Unkwang' could have higher head rice percentage and head rice yield. Therefore, if you transplant rice before June 24 in rice-wheat double cropping system, 'Hyunpum' is recommended as adequate rice cultivar. But if you transplant rice after June 24, further research is needed to find out adequate rice cultivar.

• Korean Journal of Agricultural and Forest Meteorology
• /
• v.25 no.2
• /
• pp.108-116
• /
• 2023

Recently, the concentration of fine dust causative substances (NOx, VOC, etc.) in the atmosphere has increased, resulting in high concentrations of tropospheric ozone (O3) and increased damage to crops. This study aimed to analyze the impact of high concentrations of ozone gas on the initial growth of rice plants and investigate the relationship between ozone damage resistance and anthocyanin biosynthesis. To achieve this, rice plants were exposed to elevated levels of ozone g as using an ozone chamber, and subsequent measurements were taken to assess changes in growth, the percentage of damaged leaves, and the anthocyanin content. The results revealed that varieties with a higher proportion of damaged leaves exhibited a relative increase in anthocyanin biosynthesis following ozone exposure. Notably, detrimental effects on growth, such as decreased biomass, were mitigated. Additionally, Anthocyanin biosynthesis genes in rice were listed by selecting homologous genes from Arabidopsis and Maize. The expression of OsF3H2, OsFLS1 and OsLDOX3 was induced during ozone treatment. This result is expected to contribute to the study of the protection mechanism of plants from ozone damage.

• The Plant Pathology Journal
• /
• v.39 no.4
• /
• pp.361-373
• /
• 2023

In plant-pathogen interactions, Magnaporthe oryzae causes blast disease on more than 50 species of 14 monocot plants, including important crops such as rice, millet, and most 15 recently wheat. M. oryzae is a model fungus for studying plant-microbe interaction, and the main source for fungal pathogenesis in the field. Here we report that MoJMJD6 is required for conidium germination and appressorium formation in M. oryzae. We obtained MoJMJD6 mutants (ΔMojmjd6) using a target gene replacement strategy. The MoJMD6 deletion mutants were delayed for conidium germination, glycogen, and lipid droplets utilization and consequently had decreased virulence. In the ΔMojmjd6 null mutants, global histone methyltransferase modifications (H3K4me3, H3K9me3, H3K27me3, and H3K36me2/3) of the genome were unaffected. Taken together, our results indicated that MoJMJD6 function as a nuclear protein which plays an important role in conidium germination and appressorium formation in the M. oryzae. Our work provides insights into MoJMJD6-mediated regulation in the early stage of pathogenesis in plant fungi.

• Parasites, Hosts and Diseases
• /
• v.53 no.4
• /
• pp.371-377
• /
• 2015

Trichomonas vaginalis induces proinflammation in cervicovaginal mucosal epithelium. To investigate the signaling pathways in $TNF-{\alpha}$ production in cervical mucosal epithelium after T. vaginalis infection, the phosphorylation of PI3K/AKT and MAPK pathways were evaluated in T. vaginalis-infected SiHa cells in the presence and absence of specific inhibitors. T. vaginalis increased $TNF-{\alpha}$ production in SiHa cells, in a parasite burden-dependent and incubation time-dependent manner. In T. vaginalis-infected SiHa cells, AKT, ERK1/2, p38 MAPK, and JNK were phosphorylated from 1 hr after infection; however, the phosphorylation patterns were different from each other. After pretreatment with inhibitors of the PI3K/AKT and MAPK pathways, $TNF-{\alpha}$ production was significantly decreased compared to the control; however, $TNF-{\alpha}$ reduction patterns were different depending on the type of PI3K/MAPK inhibitors. $TNF-{\alpha}$ production was reduced in a dose-dependent manner by treatment with wortmannin and PD98059, whereas it was increased by SP600125. These data suggested that PI3K/AKT and MAPK signaling pathways are important in regulation of $TNF-{\alpha}$ production in cervical mucosal epithelial SiHa cells. However, activation patterns of each pathway were different from the types of PI3K/MAPK pathways.

• Journal of agriculture & life science
• /
• v.46 no.4
• /
• pp.1-8
• /
• 2012

Acorn production of some oak species from 2008 to 2010 in Mt. Jiri national park(Pia, Bitjeom, Jangdang, Baemsa, Hansin, and Chilsun) was measured and compared among study area, altitude and aspect because acorns are an important wildlife food resources. Acorns were produced the most in Jangdang and Bitjeom area at year of good fruiting, and those of Bitjeom and Hansin area were produced the most at bad fruiting year. In terms of altitudinal characteristics on acorn production, acorn production in 1000 and 900 m was the most at good fruiting year, and 800 and 900 m at bad fruiting year showed the most production of acorn. Throughout the study period, acorn production on southern slopes was larger than that of northern slopes.

• Asian-Australasian Journal of Animal Sciences
• /
• v.12 no.3
• /
• pp.445-448
• /
• 1999

Beef and dairy cattle are the primary ruminant livestock in Korea, but there is a serious shortage of both fresh and dry forages. Small areas of forage crops or pastures, unfavorable soil and climatic conditions, high costs for pasture production, low establishment and management technologies, etc. are the main factors limiting roughage production in Korea. To meet the roughage demand of cattle several suggestions are presented. About 845,000 ha of hilly area could be developed for pastureland. Almost the same area of paddy field, and more than 200,000 ha of reclaimed land could be used for pasture production. If all the potential area is developed for pastureland, the area could be extended to 10 times more than is presently used for pasture crops and pastureland. Productivity would be increased by developing new technologies of establishment and management. Silvo-pastoral systems should be introduced to Korea.

• 한국생물공학회:학술대회논문집
• /
• 2005.10a
• /
• pp.260-265
• /
• 2005

In the previous research about ethanol production, we confirmed that SFW(saccharified foodwastes) medium(0.56g-ethanol/g-glucose) is mere efficient than YM medium(0.538g-ethanol/g-glucose). Ethanol production using SFW needs large enzyme cost due to the enzymatic hydrolysis of foodwastes, although the enzymes was obtained from our economical enzyme production methods, using the intact whole culture broth of Trichoderma harzianum FJ1. Therefore, in this research we used synchronous saccharification and fermentationmethod to produce ethanol using foodwastes. Ethanol production yield was 0.45g-ethanol/g-reducing sugar in synchronous saccharification and for-mentation by a fed-batch mode.