• Proceedings of the Zoological Society Korea Conference
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• 1998.04a
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• pp.22-23
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• 1998

No Abstract, See Full Text

• Safety and Health at Work
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• v.13 no.1
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• pp.131-133
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• 2022

• Molecules and Cells
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• v.40 no.2
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• pp.100-108
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• 2017

Cathepsin F, which is encoded by CTSF, is a cysteine proteinase ubiquitously expressed in several tissues. In a previous study, novel transcripts of the CTSF gene were identified in the crab-eating monkey deriving from the integration of an Alu element-AluYRa1. The occurrence of AluYRa1-derived alternative transcripts and the mechanism of exonization events in the CTSF gene of human, rhesus monkey, and crabeating monkey were investigated using PCR and reverse transcription PCR on the genomic DNA and cDNA isolated from several tissues. Results demonstrated that AluYRa1 was only integrated into the genome of Macaca species and this lineage-specific integration led to exonization events by producing a conserved 3' splice site. Six transcript variants (V1-V6) were generated by alternative splicing (AS) events, including intron retention and alternative 5' splice sites in the 5' and 3' flanking regions of CTSF_AluYRa1. Among them, V3-V5 transcripts were ubiquitously expressed in all tissues of rhesus monkey and crab-eating monkey, whereas AluYRa1-exonized V1 was dominantly expressed in the testis of the crab-eating monkey, and V2 was only expressed in the testis of the two monkeys. These five transcript variants also had different amino acid sequences in the C-terminal region of CTSF, as compared to reference sequences. Thus, species-specific Alu-derived exonization by lineage-specific integration of Alu elements and AS events seems to have played an important role during primate evolution by producing transcript variants and gene diversification.

• Molecules and Cells
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• v.38 no.11
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• pp.950-958
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• 2015

BCS1L gene encodes mitochondrial protein and is a member of conserved AAA protein family. This gene is involved in the incorporation of Rieske FeS and Qcr10p into complex III of respiratory chain. In our previous study, AluYRa2-derived alternative transcript in rhesus monkey genome was identified. However, this transcript has not been reported in human genome. In present study, we conducted evolutionary analysis of AluYRa2-exonized transcript with various primate genomic DNAs and cDNAs from humans, rhesus monkeys, and crabeating monkeys. Remarkably, our results show that AluYRa2 element has only been integrated into genomes of Macaca species. This Macaca lineage-specific integration of AluYRa2 element led to exonization event in the first intron region of BCS1L gene by producing a conserved 3' splice site. Intriguingly, in rhesus and crabeating monkeys, more diverse transcript variants by alternative splicing (AS) events, including exon skipping and different 5' splice sites from humans, were identified. Alignment of amino acid sequences revealed that AluYRa2-exonized transcript has short N-terminal peptides. Therefore, AS events play a major role in the generation of various transcripts and proteins during primate evolution. In particular, lineage-specific integration of Alu elements and species-specific Alu-derived exonization events could be important sources of gene diversification in primates.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.17 no.11
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• pp.248-255
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• 2016

The amount and diversity of information is increasing, as is the information integration connecting data among the related institutions. In the defense field, DTAQ, which is in charge of the quality of military supplies, is attempting to collect and analyze the information which is related with it. In addition, the object of information integration is to expand civil data as well as defense data. There are many ways to integrate data in various environments. In the defense field, which needs enhanced security, it is necessary to establish and apply the information integration methods which are enhanced with more security. In this study, the framework and architecture of information integration was designed by considering task requirements and security conditions. As a result of example application of this framework for information systems to the selected 4 institutions, it was confirmed that the task can be performed through data connections. From the study result, integration architecture which can be applied securely in defense field was suggested. The data accumulated by using this framework with strengthened security are expected to be utilized for the quality improvement of military supplies.

• The Journal of the Korea Contents Association
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• v.12 no.6
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• pp.518-529
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• 2012

Integration of Europe was started when European Union Treaty was concluded at Maastricht for the first time on December, 1991. Europe which may be called as a cradle of modern national state has realized a single Europe not only in the socio-economic integration field but also in the political field. Under this background, it is considered that life-long education policy for developing a new integrated growth engine of EU requires educational response that may get ready for socio-economic environmental transformation more than anything else. In particular, this policy is faced with an important task of having to achieve harmony of efficiency through diversity and mutual coordination in pursuing cooperation and integrated development of life-long education field. However, notwithstanding their efforts, since 2008, some countries of EU were faced with economic crisis due to economic recession and this situation starts to drive the whole Europe even to the point of their financial crisis at last. This crisis is currently shaking socio-economic integration of EU. This study intends to observe a status of establishing life-long education system and promoting a policy for socio-economic integration of EU and to analyze as to what kind of relevance adult participation rate of life-long learning among the countries belonged to EU has with per capita income and to explore as whether socio-economic integration among member countries could be sustained based on problems of integrative life-long education system under the economic crisis of EU. In addition, through this study, an implication required for presenting a new paradigm conversion, policy establishment and development direction for the life-long education of our country is intended to be deduced.

• Structural Engineering and Mechanics
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• v.14 no.6
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• pp.713-732
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• 2002

A local radial point interpolation method (LRPIM) based on local residual formulation is presented and applied to solid mechanics in this paper. In LRPIM, the trial function is constructed by the radial point interpolation method (PIM) and establishes discrete equations through a local residual formulation, which can be carried out nodes by nodes. Therefore, element connectivity for trial function and background mesh for integration is not necessary. Radial PIM is used for interpolation so that singularity in polynomial PIM may be avoided. Essential boundary conditions can be imposed by a straightforward and effective manner due to its Delta properties. Moreover, the approximation quality of the radial PIM is evaluated by the surface fitting of given functions. Numerical performance for this LRPIM method is further studied through several numerical examples of solid mechanics.

• Journal of Positioning, Navigation, and Timing
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• v.5 no.2
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• pp.47-57
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• 2016

Since the Global Navigation Satellite System (GNSS) may not provide navigation information due to external interferences, many countries have plans to prepare a backup system for this situation. One of the possible GNSS backup systems is a multiple radio positioning integration system in combination with the terrestrial radio navigation system. Before constructing such a GNSS backup system, M&S software is needed to analyze if the system satisfies the performance the required navigation performance. This study presents requirements of M&S software for coverage analysis of the navigation system, and proposes an M&S software design scheme on the basis of the requirements. The M&S software is implemented, and coverage analysis is performed to verify the validity of the proposed design scheme.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.43 no.2
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• pp.159-168
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• 2010

This paper presents a mathematical model and simulation method for investigating the performance of set net systems and fish cage systems influenced by currents and waves. Both systems consist of netting, mooring ropes, a floating collar and sinkers. The netting and ropes were considered flexible structures and the floating collar was considered an elastic structure. Both were modeled on a mass-spring model. The structures were divided into finite elements and mass points were placed at the mid-point of each element, and the mass points were connected by mass-less springs. Each mass point was subjected to external and internal forces and the total force was calculated at every integration step. An implicit integration scheme was used to solve the nonlinear dynamic system. The computation method was applied to dynamic simulation of actual systems simultaneously influenced by currents and waves in order to evaluate their practicality. The simulation results improved our understanding of the behavior of the structure and provided valuable information concerning the optimized design of set net and fish cage systems exposed to an open ocean environment.

• Proceedings of the Korean Society of Plant Biotechnology Conference
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• 2004.10a
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• pp.62-65
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• 2004

To protect the watermelon against soil-borne pathogens, we are currently producing disease-resistant transgenic root stock for the growth of watermelon, A defensin gene (J1-1) from Capsicum annum, a ACC deaminase gene from Pseudomonas syringae, a galactinol synthase (CsGolS) gene from Cucumis sativus, and a WRKY (CvWRKY2) gene from Citullus vulgaris were used as transgenes for disease resistance. The gene were transformed into a inbred line (6-2-2) of watermelon, Kong-dae watermelon and a inbred line (GO702S) of gourd, respectively, by Agrobacterium-mediated transformation. Putative transgenic plants were selected in medium containing 100mg/L kanamycin, and then integration of the genes into the genomic DNA were demonstrated by PCR analysis. Successful integration of the gene in regenerated plants was also confirmed by PCR (Figf 1), genomic Southern blot (Fig 2), RT-PCR (Fig 3), and Northern blot analysis(Fig 4). Several T1 lines having different transgene were produced, and disease resistance of the T1 lines are under estimation.