• Applied Science and Convergence Technology
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• v.25 no.3
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• pp.61-65
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• 2016

Here, the rapid detection of Salmonella typhimurium by a portable surface plasmon resonance (SPR) biosensor in which the beam from a diode laser is modulated by a rotating mirror is reported. Using this system, immunoassay based on lipopolysaccharides (LPS)-specific monoclonal anti-Salmonella antibody was performed. For the purpose of orientation-controlled immobilization of antibodies on the SPR chip surface, the cysteine-mediated immobilization method, which is based on interaction between a gold surface and a thiol group (-SH) of cysteine, was adopted. As a result, using the portable SPR-based immunoassay, we detected S. typhimurium in the range from 10^7 CFU/mL to 10^9 CFU/mL within 1 hour. The results indicate that the portable SPR system could be potentially applied for general laboratory detection as well as on-site monitoring of foodborne, clinical, and environmental agents of interest.

• The Transactions of The Korean Institute of Electrical Engineers
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• v.59 no.5
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• pp.922-926
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• 2010

We have investigated the grating coupled surface plasmon resonance (GC-SPR) sensors using ZnO nano-grating structures to enhance the sensitivity of an SPR sensor. The GC-SPR sensors were analyzed using the finite-difference time-domain method. The optimum resonance angles of 49 degrees are obtained in the 150 nm wide grating structure with a period of 300 nm for the ZnO thickness of 30 nm. Then, the ZnO nano-grating patterns were fabricated by using laser interference lithography. The measured resonance angle of nano-grating patterns was around 49 degrees. Here, an enhanced evanescent field is obtained due to the surface plasmon on the edge of the bandgap when the ZnO grating structures are used to excite the surface palsmon.

• Journal of Sensor Science and Technology
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• v.18 no.4
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• pp.251-262
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• 2009

Biosensors exploit the specific binding between recognition molecule on the biosensor surface and target molecule in analyte and are used in the detection of specific biomolecules such as protein, DNA, cell, virus, etc., with a view towards developing analytical devices. Recently, application field of biosensors have been expanding from diagnosis to biodefense because they can basically serve as high performance devices. This review describes the basic information of biosensors including definition, classification, and operational principle. Moreover, we introduce micro/nano technology-based biosensors with better detection performance than traditional method and their application examples.

• Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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• 2008.06a
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• pp.356-357
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• 2008

The effects of $O_2$ plasma ashing process for surface treatment of nano-wire Bio-FET were investigated. In order to evaluate the plasma damage introduced by $O_2$ plasma ashing, a back-gate biasing method was developed and the electrical characteristics as a function of $O_2$ plasma power were measured. Serious degradations of electrical characteristics of nano-wire Bio-FET were observed when the plasma power is higher than 50 W. For curing the plasma damages, a forming gas anneal (2 %, $H_2/N_2$) was carried out at $400^{\circ}C$. As a result, the electrical characteristics of nano-wire Bio-FET were considerably recovered.

• Journal of the Korean Society for Precision Engineering
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• v.25 no.11
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• pp.15-21
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• 2008

• Transactions of the Korean Society of Mechanical Engineers A
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• v.32 no.6
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• pp.459-464
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• 2008

We present a novel microcantilever device with nano-interdigitated electrodes (nano-IDEs) and DNA selective immobilization on the nano-IDEs for biosensing applications. Using the nano-IDEs and cyclic voltammetric methods, we have achieved selective immobilization of DNA with submicrometer spatial resolution on a freestanding microcantilever. $70{\sim}500\;nm$-wide gold (Au) nano-IDEs are fabricated on a low-stress SiNx microcantilever with dimensions of $100{\sim}600\;{\mu}m$ in length, and $15{\sim}60\;{\mu}m$ in width, with a $0.5\;{\mu}m$ thickness using electron beam lithography and bulk micromachining. Streptavidin is selectively deposited on one side of the nano-IDEs using cyclic voltammetry at a scan rate of 0.1 V/s with a range of $-0.2{\sim}0.7\;V$ during $1{\sim}5$ cycles. The selective deposition of dsDNA is confirmed by fluorescence microscopy after labeling with YOYO-1 dye.

• Journal of Microbiology and Biotechnology
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• v.17 no.11
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• pp.1848-1855
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• 2007

Antibacterial activity of essential oils (Tea tree, Chamomile, Eucalyptus) on Staphylococcus aureus growth was evaluated as well as the essential oil-loaded alginate beads. The binding interactions between the cell and the essential oils were measured using an optical biosensor. The antibacterial activity of the essential oils to the cell was evaluated with their binding interaction and affinity. The antibacterial activity appeared in the order of Tea Tree>Chamomile>Eucalyptus, in comparison of the inhibition effects of the cell growth to the essential oils. The association rate constant and affinity of the cell binding on Tea Tree essential oil were $5.0{\times}10^{-13}\;ml/(CFU{\cdot}s)$ and $5.0{\times}10^5\;ml/CFU$, respectively. The affinity of the cell binding on Tea Tree was about twice higher than those on the other essential oils. It might be possible that an effective antibacterial activity of Tea Tree essential oil was derived from its strong adhesive ability to the cell, more so than those of the other essential oils.

• Applied Chemistry for Engineering
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• v.27 no.6
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• pp.565-572
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• 2016

While there have been great demands on improving domestic water pollution issues, the necessity for real time monitoring of particular drug residues in water resources has been raised since drug residues including antibiotics could provoke new trains of drug-resistant bacteria in water environments. Among many different types of drugs used for pharmaceutical treatment, antibiotics are considered to be one of the most hazardous to our ecosystem since they can rapidly promote the spreading of drug-resistant bacteria in water environments. In this mini-review, we will highlight recent developments made on creating in-situ sensing platforms for the fast monitoring of antibiotic residues in aquatic environmental samples focusing on optical and electrochemical techniques. Related recent technology developments and the resulting economy effects will also be discussed.

• Journal of the Korean Electrochemical Society
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• v.10 no.2
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• pp.150-154
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• 2007

Redox complexes to transport electrodes from biomaterial to electrodes are very important part in commercial biosensor industry. A novel osmium redox complex was synthesized by the coordinating pyridine group with osmium metal. A novel osmium complex is described as $[Os(dme-bpy)_2(ap-im)Cl]^{+/2+}$. We have been studied the electrochemical characteristics of this osmium complex with electrochemical techniques such as cyclic voltammetry and chronoamperommetry. In order to immobilize osmium redox complexes on the electrode, we deposited gold nano-particles on screen printed carbon electrode(SPE). The electrical signal converts the osmium redox films into an electrocatalyst for glucose oxidation. The catalytic currents were monitored that the catalytic currents were linearly increased from 1 mM to 5 mM concentrations of glucose.

• Applied Chemistry for Engineering
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• v.27 no.2
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• pp.145-152
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• 2016

In this study, CuO was introduced on MWCNTs dispersed with Au nanoparticles to improve the glucose sensing capability of electrochemical biosensors. Nano-cluster shaped CuO was synthesized due to the presence of Au nanoparticle, which affects glucose sensing performance. The biosensor featuring CuO/Au@MWCNTs nanocomposite as an electrode material when 0.1 mole of CuO was synthesized showed the highest sensitivity of $504.1{\mu}A\;mM^{-1}cm^{-2}$, which is 4 times better than that of MWCNTs based biosensors. In addition, it shows a wider linear range from 0 to 10 mM and lower limit of detection (LOD) of 0.008 mM. These results demonstrate that CuO/Au@MWCNTs nanocomposite sensors are superior to other CuO based biosensors which are attributed that the nano-cluster shaped CuO is favorable for the electrochemical reaction with glucose molecules.