• Mycobiology
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• v.28 no.3
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• pp.119-122
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• 2000

Phytases (myo-inositol hexakisphosphate phosphohydrolase; EC 3.1.3.8) are enzymes which catalyze the hydrolisys of phytate into myo-inositol and inorganic phosphates. Phytases are found in plants and a variety of microorganisms. Aspergillus species were treated with 254 nm of UV irradiation for the screening of phytase overproducing mutant strains. At 15 minute irradiation, the survivals of population were less than 5%, and UV irradiation time was decided at 20 minute for the isolation of mutant strains. Four UV mutant strains in A. oryzae (YUV-47, -169, -341, -511) and six in A. ficuum (FUV-17, -36, -69, -193, -317, -419) were isolated on PSM media containing ammonium phosphate. The specific enzyme activities of A. ficuum mutants are 110 to 140% higher than that of wild type.

• The Korean Journal of Mycology
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• v.27 no.4 s.91
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• pp.299-303
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• 1999

Phytase(myo-inositol-hexakis phosphate 3-phosphohydrolase, E C 3.1.3.8) sequentially hydrolyzes phytate to myo-inositol and inorganic phosphate. Phytase of Aspergillus ficuum was purified to homogeneity using ultrafiltration, cation exchange column and anion exchange column. It's molecular weight is estimated as around 90,000 by SDS-PAGE. Antibody against the phytase was produced by immunizing mice with the purified phytase. The titer of the antibody was determined to be 1/25,000.

• Nutritional Sciences
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• v.8 no.3
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• pp.189-195
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• 2005

To assess the effect of pinitol supplementation and strength training for two weeks on the anaerobic capacity during and after exercise, and improvement of glucose metabolism during the recovery period of muscular fatigue with repeated acute bouts of cycling exercise, a total of 24 healthy young men were recruited and randomly and equally divided into three groups; pinitol supplementation group (PSG), placebo group (PLG), and control group (CON). Using a randomized double-blinded design, subjects in PSG were provided pinitol supplement, consumed orally 1.2 g/day, and participated in the resistance exercise program and cycling exercise for two weeks. Subjects in PLG underwent the same protocol as those in PSG but consumed the same amount of placebo. No supplementation and exercise program was given to CON. Before and after the intervention, all subjects were tested for their anaerobic capacities evaluated by Wingate test twice separated by 30 min. During the test, peak anaerobic power (PP), mean anaerobic power, total work, and fatigue index were evaluated During resting and recovery, blood samples were drawn and plasma pinitol, myo-inositol, chiro-inositol, insulin, free fatty acid, glucose, and lactate levels were analyzed After two weeks, PP and relative PP of the second biking were improved from the first biking in PSG only (p<0.05). No changes were found in all other variables of Wingate test in all groups. No statistical differences between groups and pre- and post-intervention were observed in concentrations of pinitol, myo-inositol, and chiro-inositol, but pinitol concentration was higher during recovery compared to the baseline in all groups and testings (p<0.05). Lactate level during recovery was higher than the resting level, but no other blood parameters were significantly changed. In conclusion, two weeks of pinitol supplementation in conjunction with short duration of anaerobic training in healthy young men did not induce any obvious benefits in terms of anaerobic capacity and energy metabolism Individual and/or population susceptibility may be one factor responsible for adopting pinitol supplementation.

• Bulletin of the Korean Chemical Society
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• v.20 no.7
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• pp.846-848
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• 1999

• Restorative Dentistry and Endodontics
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• v.21 no.1
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• pp.366-374
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• 1996

The purpose of this study was to investigate whether D-myo-inositol-l,2,6-trisphosphate (PP56) can effectively antagonize vasoconstriction caused by neuropeptide Y in the dental pulp, and to understand involvement of neuropeptide Y in the regulation of microcirculation in the dental pulp with the aim of elucidating neurogenic inflammation. Experiments were performed on 7 cats anesthetised with sodium pentobarbital, and neuropeptide Y and a neuropeptide Y antagonist PP56 were injected close intra-arterially into the dental pulp. The probe of laser Doppler flowmeter was placed on the buccal surface of ipsilateral canine teeth to the drug administration and pulpal blood flow was measured. Intra-arterial injection of neuropeptide Y (1.3-$2.0\;{\mu}g$/kg) resulted in pulpal blood flow decrease of $37.73{\pm}5.73%$(mean${\pm}$SEM) (n=9). Intra-arterial injection of PP56(0.3 mg/kg) alone changed pulpal blood flow little by 1.03 % reduction. The effect of neuropeptide Y in the presence of PP56 resulted in significantly less decreases in pulpal blood flow ranging from $27.17{\pm}5.37$ to $16.63{\pm}3.48%$ from control as compared with neuropeptide Y alone(n = 13). In effect, PP56 attenuated pulpal blood flow caused by neuropeptide Y. Results of the present study have provided evidences that a non-peptide PP56 is capable of antagonizing vasoconstriction caused by neuropeptide Y in the feline dental pulp. In addition, they show functional evidences that neuropeptide Y plays an active role in modulating the microcirculation of the dental pulp.

• Proceedings of the Korean Society of Fisheries Technology Conference
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• 2002.10a
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• pp.179-180
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• 2002

Phytase는 phytic acid (myo-inositol 1,2,3,4,5,6 hexakis dihydrogen phosphate)를 분해하여 phosphate와 phosphate inositol를 만드는 효소이다. Phytic acid는 가축의 사료로 사용하고 있는 곡물의 경우 인함량의 50∼70％를 차지하지만, 물고기, 닭, 돼지와 같은 단위동물은 생체 내에 phytic acid를 분해하는 phytase가 존재하지 않기 때문에 식물성 인의 이용률이 극히 낮아서 성장에 필요한 인을 무기물 형태로 외부에서 따로 충분히 공급해줘야만 한다. (중략)

• Journal of Mushroom
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• v.10 no.4
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• pp.216-223
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• 2012

Oyster mushroom is one of the popular mushrooms for Korean people and it is thus one of the mushrooms that were mostly cultivated in Korea in addition to winter and king oyster mushrooms. To promote the consumption in terms of its nutritional value and determine the absorption mechanism in its physiological and metabolic aspects, we investigated the sugar composition of its fruit bodies according to the amount of sugar component in the medium. As a result from the treatment of 4 different sugar components to the sawdust medium of oyster mushroom varieties (i.g., Suhan 1 ho, Yeorum, Chunchu 2 ho), we detected fructose, glucose, ribose, xylose as a monosaccharide with ${\alpha}$-lactose and trehalose as a disaccharide. The sugar alcohols were also detected including glycerol, mannitol, myo-inositol, and sorbitol. The sugar components that were detected in all treatments were trehalose, mannitol, ${\alpha}$-lactose, fructose, glucose, ribose, and myo-inositol and the rest of the components were found in some treatments with their different quantity included in the fruit bodies. The amount of monosaccharides included in fruit bodies is comparatively low and Suhan 1 ho contained them more than other varieties. There was no remarkable difference in the amount of sugar components in fruit bodies depending on treated sugars and their amounts in the treatment. With the increase of lactose treatment, the sugar components were markedly increased and the amount of trehalose in the fruit bodies was different according to the varieties. Therefore, it is not possible to detect the trend of sugar components according to the medium used in the cultivation. The sugar alcohols in the fruit bodies were comparatively contained more in Suhan 1 ho and Chunchu 1 ho. The amount of mannitol in the fruit bodies was not possible to be detected in terms of its trend according to the treatment. For myo-inositol, its quantity is gradually increasing in Suhan 1 ho and Chunchu 1 ho and it is not possible to detent the trend in Yeorum. In conclusion, we only detected ${\alpha}$-lactose as a sugar component that showed a correlation between the amount of treatment and the amount of the fruit bodies. Therefore, the sugar components detected in the fruit bodies were transformed rather than direct absorption into fruit bodies and further studies are needed to address this question.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.41 no.6
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• pp.729-735
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• 1996

This study was carried out to investigate the effect of Germaniwn(Ge) treatment in the culture media on the Ge absorption by the callus induced from brown rice cv. Dongjinbyeo. MS medium was more effective on the growth ratio of callus and the content of Ge and some inorganic elements except K in callus than N$_{6}$ medium. The more Ge treatment in the N$_{6}$ or MS medium, the more Ge absorption by the callus, but the growth ratio of callus and the content of Ca, Mg, and K in callus were decreased. The content of Fe, Mn, Zn, and Cu was increased under treatment up to 100～200mg /$\ell$ Ge, but tended to be decreased under treatment more than that of Ge concentration. Under treatment less than 150mg /$\ell$ Ge, GeO$_2$(inorganic Ge) was more effective on the Ge absorption by callus than Ge-132(organic Ge), but Ge-132 was more effective on the Ge ab-sorption by callus and the activity of callus in case of treatment more than 150mg /$\ell$ Ge. The lower pH of culture medium, the higher Ge content in the callus. When callus was cultured on medium supplemented with Ge and 0.1～1.0mM of citric acid or myo-inositol, content of Ge and some inorganic elements in callus, as well as growth and dry weight of callus, were tend to increase in comparison to control, but myo-inositol was more effective on them than citric acid.cid.

• BMB Reports
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• v.38 no.1
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• pp.58-64
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• 2005

Myo-inositol monophosphate phosphatase (IMPP) is a key enzyme in the phosphoinositide cell-signaling system. This study found that incubating the IMPP from a porcine brain with pyridoxal-5'-phosphate (PLP) resulted in a time-dependent enzymatic inactivation. Spectral evidence showed that the inactivation proceeds via the formation of a Schiff's base with the amino groups of the enzyme. After the sodium borohydride reduction of the inactivated enzyme, it was observed that 1.8 mol phosphopyridoxyl residues per mole of the enzyme dimer were incorporated. The substrate, myo-inositol-1-phosphate, protected the enzyme against inactivation by PLP. After tryptic digestion of the enzyme modified with PLP, a radioactive peptide absorbing at 210 nm was isolated by reverse-phase HPLC. Amino acid sequencing of the peptide identified a portion of the PLP-binding site as being the region containing the sequence L-Q-V-S-Q-Q-E-D-I-T-X, where X indicates that phenylthiohydantoin amino acid could not be assigned. However, the result of amino acid composition of the peptide indicated that the missing residue could be designated as a phosphopyridoxyl lysine. This suggests that the catalytic function of IMPP is modulated by the binding of PLP to a specific lysyl residue at or near its substrate-binding site of the protein.

• Applied Chemistry for Engineering
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• v.5 no.4
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• pp.573-579
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• 1994

1-O-oleoyl glycerol, 2-O-oleoyl myo-inositol and methyl 2-O-oleoyl-${\alpha}$-D-glucopyranoside were used as surfactants in this study. The solution properties and solubilization process of those nonionic surfactants were examined by the phase equilibria. As a result of this study, we have found that phase behavior of two component systems of surfactants/$H_2O$/cyclohexane depends on temperature respectively. The three phase regions of three component systems appeared in the temperature range of $27^{\circ}C{\sim}32^{\circ}C$, $36^{\circ}C{\sim}45^{\circ}C$ and $38^{\circ}C{\sim}52^{\circ}C$ and solubilization of water and oil was high in those three phase ranged As the temperature was varied in the two component systems, liquid crystals of hexagonal were observed to in the case of 1-O-oleoyl glycerol, and liquid crystal of lamella types were observed in the case of 2-O-oleoyl myo-inositol and methyl 2-O-oleoyl-${\alpha}$-D-glucopyranoside.