• Title/Summary/Keyword: mycology

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Historical Studies on Scientific Research of Mycologist Sam Soon KIM (균학자 김삼순 연구활동의 과학사적 접근)

  • You-Jeong Sun;Geun Bae Kim
    • The Korean Journal of Mycology
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    • v.50 no.2
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    • pp.75-92
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    • 2022
  • Sam Soon KIM was a female scientist who pioneered mycology in South Korea. She not only created the Korean Society of Mycology and the Korean Journal of Mycology but also achieved extraordinary results in the studies of mycology. After returning from Kyushu University in Japan in 1966, she turned her attention to the applied research of microorganisms. Kim pursued an earnest exploration of the practical values of bacteria in addition to those of fungi and mushrooms. Then, with the founding of the Korean Society of Mycology in 1972, she emerged as the central figure in the rare academia of mycology. Particularly, mushroom research, which had been stagnant, became revitalized by the joining of researchers from the Rural Development Administration. Taking this as momentum, Kim moved beyond applied research, such as mushroom cultivation, and led the mushroom name unification plan from 1978. She also studied mushroom classification and eventually launched publishing an illustrated book of mushrooms. These fruits of her long-term research trajectory led her to be known as a mushroom expert.

Cloning and Expression of Leu 2 Gene (${\beta}-isopropylmalate$ dehydrogenase) from the Basidiomycete Flammulina velutipes in E. coli (팽나무버섯 균사체에서 ${\beta}-isopropylmalate$ dehydrogenase(Leu 2) gene 의 cloning 및 E. coli에서 발현)

  • Byun, Myung-Ok;Yoo, Young-Bok;Go, Seung-Joo;You, Chang-Hyun;Cha, Dong-Yul;Park, Yong-Hwan
    • The Korean Journal of Mycology
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    • v.17 no.1
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    • pp.35-38
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    • 1989
  • Gene libraries of DNA from Flammulina velutipes were constructed using Escherichia coli plasmid pBR 322. Leu 2 gene coding ${\beta}-isopropylmalate$ dehydrogenase from F. velutipes was cloned by complementation of leucine requiring mutant of E. coli. The size of inserted DNA fragment of this clone is about 1 Kbp. The fragment has Bam H1 and Ava 1 restriction sites.

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Studies on Sclerotium Formation in Curvularia Species

  • Singh, U.P.;Singh, S.K.;Sugawara, Koya;Srivastava, J.S.;Sarma, B.K.;Prithiviraj, B.
    • Mycobiology
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    • v.29 no.3
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    • pp.154-159
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    • 2001
  • Natural sclerotium formation in two species of Curvularia was observed. The sclerotia were spherical to elongated. The scanning electron microscopical observations of sclerotia revealed that the sclerotia were of two distinct layers of cells, outer loosely woven hyphae and inner contact layer of cells. Different lights, viz. red, blue, green, fluorescent or addition of culture filtrate of Sclerotium rolfsii in the medium did not affect sclerotium formation.

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Microscopic observation of conidia from the genus of Pleurotus (느타리버섯속(屬)에서 Conidia의 현미경적(顯微鏡的) 관찰(觀察))

  • Byun, Myung-Ok;Yoo, Young-Bok;Go, Seung-Joo;You, Chang-Hyun;Cha, Dong-Yeul
    • The Korean Journal of Mycology
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    • v.19 no.1
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    • pp.27-31
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    • 1991
  • Formation of conidium, an asexual organ on the hyphae, was examined from ten species of Pleurotus. Conidiospores of them were distinguished into two types of spores; an elliptical and a globose spores. Dikaryotic hyphae of ten species and monokaryotic hyphae of three species were observed to produce conidiospore. Conidia were observed on the hyphae grown on mushroom complete agar medium but were not on mushroom minimal agar medium. Two aconidial mutants were obtained by the ultraviolet irradiation.

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Factors Affecting Fusion Frequency of Pleurotus Protoplasts (느타리버섯속(屬)의 원형질체(原形質體) 융합률(融合率)에 영향(影響)을 미치는 요인(要因))

  • Yoo, Young-Bok;Kim, Yeong-Tae;Byun, Myung-Ok;You, Chang-Hyun;Cha, Dong-Yeul;Park, Yong-Hwan
    • The Korean Journal of Mycology
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    • v.18 no.2
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    • pp.77-83
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    • 1990
  • Factors influencing the fusion frequency of protoplasts were investigated with auxotrophic mutants of Pleurotus florida and Pleurotus ostreatus. Immediately after the polyethylene glycol (PEG) solution was added, the protoplasts adhered firmly and shrank. During the subsequent dilution with 0.6 M sucrose, the protoplasts regained their normal size and larger bodies were observed. Interspecific heterokaryons were obtained by fusion of the nutritionally complementing protoplasts. Hyphae of the heterokaryotic fusants formed true clamp connections. The optimum conditions were a total of 1 to 15 million protoplasts per ml, 30% polyethylene glycol 8000 solution with adjustment to pH 8.0 and 0.6 M sucrose stabilized regeneration medium. Other parameters such as $CA^{++}$, glycine, exposure time and temperature influenced mainly the viability of the protoplasts.

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