• 제목/요약/키워드: muscle cell culture.acetylcholinesterase

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인삼의 dammarane계 glycosides 분획물이 일차 배양한 계배의 근육세포에 미치는 영향 (The Effect of Dammarane Glycosides of Panax ginseng on Primary Cultured Chicken Embryonic Muscle Cells)

  • 정영경;박미정;송진호;김영중
    • 약학회지
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    • 제33권3호
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    • pp.161-166
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    • 1989
  • Effects of dammarane glycosides of Panax ginseng on primary cultured chicken embryonic skeletal muscle cells were studied by microscopic observation and determination of the activity of acetylcholinesterase. Muscle cells were prepared from the breast of 12-day-old chicken embryo and cultured with either a medium consisted of 87.5% Dulbecco's Modified Eagle Medium (DMEM), 10% horse serum and 2.5% chicken embryonic extract or a medium consisted of 90% DMEM and 10% horse serum. It was observed that dammarane glycosides of Panax ginseng seemed to show the tendency to stimulate the growth and the differentiation of the muscle cells cultured with a medium consisted of 90% DMEM and 10% horse serum under microscopic observation. The activity of acetylcholinesterase in the muscle cells cultured with a medium consisted of 90% DMEM and 10% horse serum was increased by dammarane glycosides of Panax ginseng.

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