• Journal of Physiology & Pathology in Korean Medicine
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• v.20 no.5
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• pp.1290-1294
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• 2006

Biflavonoid (1) showed no antimicrobial activity at a concentration of 150 ${\mu}$g/disc. However, the crude extract of Quintinia acutifolia Kirk inhibited the growth of Bacillus subtilis and the dermatophytic fungus Trichophyton mentagrophytes. 2',3'-Dihydroochanaflavone (1) showed some cytotoxicity with IC$_{50}$ value of 3.1 ${\mu}$g/mL against P388 murine lymphocytic leukemia cells (positive control: mitomycin C IC$_{50}$ 0.06 ${\mu}$g/mL). The structure was determined by Spectroscopic methods.

• Korean Journal of Veterinary Service
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• v.26 no.3
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• pp.203-214
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• 2003

Scrub typhus and murine typhus are common endemic febrile illness in the fall in Korea. Scrub typhus is caused by Orientia tsutsugomushi, murine typhus is caused by Rickettsia typhi. Trombiculid mites are known as both the vector and the reservoir host of O tsutsugamushi, the mites which transmit O tsutsugomushi have been reported to be Leptotrombidium pallidum and L scutellare. The author carried out an epidemiological study of scrub typhus and murine typhus in Kyodong-Myeon, Kanghwa-Gun, Incheon in relation to the residents and the host rodents, such as their distribution, seroepidemiology, and population density of chigger mites. 1. Out of 900 residents, 33(3.7%) showed positive reaction to O tsutsugamushi, 24(2.7%) to R typhi. 2. In the seropositives to O tsutsugamushi or R typhi, between the sixties and the seventies of the age were dominant. 3. In the seropositives to O tsutsugamushi serotypically Gilliam was dominant. 4. Among the total 42 field rodents trapped by the sherman traps, 18 rodents were Apodemus agrarius(42.9%), 13 rodents were Crocidura lasiura(31.0%), 5 rodents were Musmusculus(11.9%), 2 rodents(4.8%) were Crocidura suaveolens, Rattus norvegicus, Tscherskia triton, respectively. 5. Out of 42 field rodents, 25 were parasitized by 4,419 chigger mites, showing 59.5% of the infestation rate and 98.8 of the chigger index. L pallidum parasitized in A agrarius, C lasiura, M musculus, R norvegicus and T triton, and L scutellare parasitized only C lasiura. 6. Antibodies in the sera of field rodents against O tsutsugamushi and R typhi were investigated by indirect immunofluorescent antibody technique. Positive rate of antibody against O tsutsugamushi were 11.9(5 of 42) and all of the positive is A agrarius. Antibody against R typhi was not detected. These results might provide the basic information for the management of scrub typhus and murine typhus in Kyodong-myeon, where the epidemiological studies on scrub typhus and murine typhus was not carried out enough.

• Journal of Digital Convergence
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• v.13 no.5
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• pp.337-344
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• 2015

Medicinal plants containing wellness-fusion-complex compound are increasingly being pursued as suitable alternative sources of various biological properties. In this study, inhibitory effect of Quintinia acutifolia, which is a New Zealand plant, on P388 murine lymphocytic leukemia cells using MTT [3-(4,5-dimethylthiazol-2-yl)- 2,5-diphenyl-tetrazolium bromide] assay. Based on $^1H-NMR$, $^{13}C-NMR$ spectral data and other spectral analysis, 2,3,2'',3''-tetrahydroochanaflavone (1) and 2'',3''-dihydroochana-flavone (3) inhibited the leukemia cells were purified from the plants. 2,3,2'',3''-tetrahydroochanaflavone (1) and 2'',3''-dihydroochana-flavone (3) are biflavonoids possessing two basic flavonoids and actively inhibited growth of P388 murine lymphocytic leukemia cells with a 50% inhibitory concentration ($IC_{50}$) of $8.2{\mu}g/mL$ and $3.1{\mu}g/mL$, respectively. Specially, 2'',3''-dihydroochana-flavone (3) possessed unconjugated flavonone system, which isn't consist of a pair with B ring of 2,3,2'',3''-tetrahydroochanaflavone (1). Therefore, the two compounds could be considered as a candidate for development of anticancer drugs and need to much studies in the future.

• Archives of Pharmacal Research
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• v.9 no.3
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• pp.175-181
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• 1986

Four technical grade organophosphate pesticides (Fenitrothion, Fenthion, Dizninon and EPN) were investigated for their effects on the murine immune function. Among the immunotoxicological assay parameters of NIEHS, humoral immune parameter and pathotoxicological indicators were examined in this study. Subchronic exposure of rodents to these pesticides resulted in marked suppression of humoral immune function and moderate histological changes of lymphoid organ any significant alterations of clinical status.

• Korean Journal of Microbiology
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• v.26 no.2
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• pp.101-105
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• 1988

6M-MuLV mutants containing deldtions around the putative packaging signal were constructed by using recombinant DNA technique and transfected into NIH/3T3 cell. 2 of 6 mutants can not be packaged into virions even in the presence of the wild type helper virus. The boundary between the packagible and the non-packagible genome is located around Pvu I site, 421 nucleotide downstream from the 5' end of M-MuLV genome. 10 base pair inverted repeat sequence (GAGUCCAAAA) which can make stem structure around Pvu Isite could be the putative packaging signal.

• Proceedings of the PSK Conference
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• 2003.04a
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• pp.214.2-215
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• 2003

Reactive oxygen species play an important role in aging. carcinogenesis, and certain neurological disorders of human beings in addition to the host-defensive mechanism of inflammatory response. Murine macrophages Raw264.7 released superoxide anions via NADPH oxidase complex and nitric oxide (NO) via iNOS synthase when the cells were stimulated with unopsonized zymosan binding to complement receptor. (omitted)

• Proceedings of the Korean Society for Food Science of Animal Resources Conference
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• 2004.05a
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• pp.318-321
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• 2004

분리동정한 유산균의 cell wall은 murine macrophage를 활성화 시켜 NO와 cytokine을 생성함을 확인하였다. 그 결과 NO 생성량에 있어서는 L. casei SGU 0020에서 가장 높은 분비를 유도하였으며, TNF-a의 분비량은 S. thermophilus SGU 0021의 균주에서 가장 높았다. 그러나 cytokine인 일종인 $IL-1{\beta}$, IL-2, IL-6에 있어서는 L. casei SGU 0020에서 가장 많은 IL의 분비를 유도하였다.

• Proceedings of the Korean Society of Toxicology Conference
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• 2002.11b
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• pp.137-137
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• 2002

Capsaicin has been reported to induce apoptosis in various cancer cells. However, its effect on bladder cancer cells has not been studied. In this study, we investigated whether capsaicin induces apoptosis in murine orthotopic bladder cancer MBT-2 cells and reactive oxydative species(ROS) are involved in capsaicin-induced apoptotic process.(omitted)

• Proceedings of the PSK Conference
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• 2003.04a
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• pp.211.1-211.1
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• 2003

There are now increasing evidences that free radicals and reactive oxygen species are involved in a variety of pathological events. Flavonoids. a group of polypenolic compounds, are widespread in the human food supply. This study was carried out to investigate the antioxidant activity of these compounds. myriceitn and (+)-catechin on B 16Fl0. murine melanoma cell line in oxidative stress. Oxidative stress was induced by exposure to hydrogen peroxide. (omitted)

• Proceedings of the PSK Conference
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• 2003.10b
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• pp.156.1-156.1
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• 2003

Cerarmide acts as a lipid second messenger in the cellular signal transduction and is involved in mediating a variety of cell functions such as proliferation, differentiation, growth arrest, and apoptosis. In the present study, we have investigated the effect of ceramide on cellular cytotoxity and reactive oxygen species (ROS) to understand the relationship between them. Ceramide treatment significantly increased cell death in RAW 264.7 murine macrophages activated with lipopolysaccharide (LPS) and interferon-g (IFN-g). (omitted)