• 제목/요약/키워드: mung bean

검색결과 245건 처리시간 0.029초

양송이배지로부터 분리한 Klebsiella michiganensis Jopap-1의 식물생장촉진효과 (Plant growth promotion effect of Klebsiella michiganensis Jopap-1 isolated from button mushroom bed)

  • 김예슬;윤민호
    • 한국버섯학회지
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    • 제16권3호
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    • pp.218-224
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    • 2018
  • An auxin-producing bacterium, Klebsiella michiganensis Jopap-1, was isolated from a button mushroom bed in Buyeo-Gun, Chungcheongnam-Do. The strain Jopap-1 was classified as a novel strain of K. michiganensis based on a chemotaxonomic and phylogenetic analysis. The isolated K. michiganensis Jopap-1 was confirmed to produce indole-3-acetic acid (IAA), which is one of auxin hormones by TLC and HPLC analyses. The maximum concentration of IAA ($96.05mg\;L^{-1}$) was detected in the culture broth incubated in R2A medium containing 0.1% L-tryptophan for 48 h at $35^{\circ}C$ by HPLC quantity analysis. A negative relationship between IAA production and pH variation was estimated to show that the increase of IAA caused acidic pH in the culture. The effect of the supplement on L-tryptophan (precursor of IAA) production was observed to be highest at 0.1% concentration, but was significantly lowered above a concentration of 0.2%. To investigate the growth-promoting effects on the crops, the culture broth of E. michiganensis Jopap-1 was infected to water cultures and seed pots of mung bean and lettuce. Consequently, the adventitious root induction and root growth of mung bean and lettuce were observed to be 2.1 and 1.8 times higher than those of the control.

녹두의 전분지질에 관한연구 (Compositions of Lipid Class and Fatty Acid in Lipids Extracted from Mung Bean Starch)

  • 엄수현;송영옥;최홍식
    • 한국식품영양과학회지
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    • 제19권1호
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    • pp.87-93
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    • 1990
  • 녹두전분으로 부터 유리지질과 저온 및 고온에서 결합지질을 추출한 다음 이들을 중성지질, 당지질, 인지질로 분획 정량하였으며 아울러 각 지질들의 지방산 조성을 분석하였다. 녹두전분에서 추출한 유리지질에서의 중성지질, 당지질, 인지질의 함량은 각각 98.2%, 1.7%, 0.1% 이였으며 저온 및 고온에서 추출한 결합지질에서는 각각 84.3~85.7%, 10.5~11.0%, 5.2~3,3% 이었다. 그리고 중성지질의 주된 성분은 triglyceride와 esterified sterol이었으며 이들의 함량은 총 중성지질의 90%를 차지 하였다. 당지질의 유리지질과 결합지질은 monogalactosyl diglycerides와 esteryl steryl glyceried가 주성분 이었다. 그리고 인지질의 경우 유리지질은 phosphatidyl ethanolamine (79.0%)이 그리고 결합지질은 phosphatidyl inositol등이 주성분이었고 고온추출 결합지질에서는 phosphatidyl giyeride 함량이 높았다. 또한 각 지질의 지방산 조성은 유리지질에서는 palmitic acid(50.2%), stearic acid(20.6%)이 주성분이었고 결합지질에서는 palmitic acid과 linoleic acid의 함량이 높았다. 그리고 유 질에서는 arachidonic acid와 behenic acid가 또한 고온추출 결합지질에서는 behenic acid가 미량 함유되어 있었다.되어 있었다.

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Effect of Transgenic Rhizobacteria Overexpressing Citrobacter braakii appA on Phytate-P Availability to Mung Bean Plants

  • Patel, Kuldeep J.;Vig, Saurabh;Nareshkumar, G.;Archana, G.
    • Journal of Microbiology and Biotechnology
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    • 제20권11호
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    • pp.1491-1499
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    • 2010
  • Rhizosphere microorganisms possessing phytase activity are considered important for rendering phytate-phosphorus (P) available to plants. In the present study, the Citrobacter braakii phytase gene (appA) was overexpressed in rhizobacteria possessing plant growth promoting (PGP) traits, for increasing their potential as bioinoculants. AppA was cloned under the lac promoter in the broadhost-range expression vector pBBR1MCS-2. Transformation of the recombinant construct pCBappA resulted in high constitutive phytase activity in all of the eight rhizobacterial strains belonging to genera Pantoea, Citrobacter, Enterobacter, Pseudomonas (two strains), Rhizobium (two strains), and Ensifer that were studied. Transgenic rhizobacterial strains were found to display varying levels of phytase activity, ranging from 10-folds to 538-folds higher than the corresponding control strains. The transgenic derivative of Pseudomonas fluorescens CHA0, a well-characterized plant growth promoting rhizobacterium, showed the highest expression of phytase (~8 U/mg) activity in crude extracts. Although all transformants showed high phytase activity, rhizobacteria having the ability to secrete organic acid showed significantly higher release of P from Ca-phytate in buffered minimal media. AppA overexpressing rhizobacteria showed increased P content, and dry weight (shoot) or shoot/ root ratio of mung bean (Vigna radiata) plants, to different extents, when grown in semisolid agar (SSA) medium containing Na-phytate or Ca-phytate as the P sources. This is the first report of the overexpression of phytase in rhizobacterial strains and its exploitation for plant growth enhancement.

1600년대-1940년대 조리서에 등장한 화채류의 종류 및 조리법 특성 (Culinary Characteristics of Hwachae in Korean Cookbooks Published in 1600-1940s)

  • 양지혜;송경희;장윤희;이영미
    • 한국식품조리과학회지
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    • 제32권6호
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    • pp.800-810
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    • 2016
  • Purpose: This study aimed to characterize the recipes of Hwachae in Korean cookbooks published from the 1600's to 1940's. Methods: We conducted a content analysis of thirteen old cookbooks (Eumsikdimibang, Jubangmun, Jeungbosallimgyeongje, Kyugonyoram, Imwonsibyukji, Gyuhapchongseo, Sieuijeonseo, Buinpilji, Chosunyorijebub, Chosunmusangsinsikyorijebub, Ganpyeonchosunyorijebub, Chosunyori, Chosunyoribub). We collected a total of 99 recipes of Hwachae and analyzed the ingredients used and characteristics of the recipes according to five groups of Hwachae: noodle type Hwachae, dumpling type Hwachae, fruit Hwachae, flower Hwachae, and the others. Results: The noodle type Hwachae included Changmeyon, Smyeon, Nanmyeon, and Waemyeon. Mung bean (81.0%) and Schisandra (95.2%) were mostly employed as solid ingredient and as beverage base, respectively. In the noodle type Hwachae, Wonsobyeong and Sudan belonged to the dumpling type Hwachae, in which mung bean (51.7%) was mostly used as solid ingredient and just water without any additive was used as the most popular beverage base (62.1%) unlike in other types of Hwachae. There were various types of fruit Hwachae, which used different fruits as solid ingredient such as pear, peach, cherry, strawberry, etc. Pears (44.4%) have been employed as the most popular ingredient and sweets such as honey and sugar have been used in all the fruit Hwachae. The flower Hwachae included Hwamyeon, Sunchaehwachae, Jangmihwachae, and Bomhwachae. In all the flower Hwachae, schizandra has been used as a beverage base. Conclusion: These findings provide basic information required for developing standard recipes of traditional Hwachae.

Antioxidative and anti-inflammatory activities of Phaseolus aureus

  • Hong, In-Taik;Kim, Beom-Jun;Yu, Duck-Cho;Kim, Jung-Haeng;Kim, Jung-Han;Heo, Moon-Young;Lee, Song-Jin;Kim, Hyun-Pyo
    • 대한화장품학회지
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    • 제22권2호
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    • pp.41-51
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    • 1996
  • Phaseolus aureus (mung bean), Leguminosae, has been used as an antidote from the ancient time. Especially, it has been widely used for cleaning face and skin in oriental countries. Although several constituents such as fatty acids, phytoallexin and phaseol derivatives were reported in P. aureus and related species including seedlings, there has been a few report to describe its biological activity. Therefore, in this investigation, the ethanol extract from P. aureus was obtained and its biological activities including the antioxidative and anti-inflammatory activities were studied. The 70% ethanol extract from P. aureus showed dose-dependent antioxidative activity (52.3% inhibition at 4 mg/ml) against lipid peroxidation assay, while the extract did not show the inhibitory activity of superoxide radical formation. The extract also showed the topical anti-inflammatory activity against croton-oil and arachidonic acid induced mouse ear edema test (18-19% inhibition at 7.5 mg/ear) as well as mild inhibitory activity against picryl chloride induced delayed hypersensitivity in mouse. For investigating active principles, vitexin and isovitexin (apigenin C-glycoside) as flavonoids, and adenosine were isolated from the extract using silica gel chromatography. The actual contents of vitexin and isovitexin were found to be 3.7 and 2.4 mg/g extract, respectively. Vitexin and isovitexin showed the antioxidative activity. They showed the topical anti-inflammatory activity, although the activities were not potent compared to the reference compounds. These results suggested that vitexin and isovitexin may be, at least in part, the compounds contributing the antioxidative activity in vitro and the topical anti-inflammatory activity of P. aureus in vivo. All results of present study might be one of the scientific rationale in using mung bean for skin care from the ancient time.

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녹두의 발아과정 중 $GA_3$ 및 ABA의 처리가 Peroxidase, Catalase 활성변화와 Isoperoxidase Pattern에 미치는 영향 (Effect of $GA_3$ and ABA on Peroxidase and Catalase Activities and Isoperoxidase Patterns in Mung Bean Seedling)

  • 이상갑;박우철
    • Applied Biological Chemistry
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    • 제31권2호
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    • pp.205-210
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    • 1988
  • 식물은 생장조절제에 의한 녹두의 부위별 peroxidase, catalase활성과 전기영동상 peroxidase 동위효소의 변화를 조사하였다. 녹두의 생육이 진행됨에 따라 하배축을 제외하고는 peroxidase 활성도는 증가하였으나 catalase 활성도는 감소하여 서로 상반된 결과를 나타내었다. 암발아시킨 녹두에 $GA_3$와 ABA를 각각 처리하면 peroxidase 활성도의 증가에 대하여 자엽에서만 $GA_3$가 ABA 보다 더 큰 영향 을 끼쳤으나 catalase 활성도 변화는 ABA가 $GA_3$ 보다 더 큰 영향을 끼쳐 모든 부위에서 활성도를 증가시켰다. $GA_3$와 ABA를 혼합하여 처리하면 효소활성도의 변화 경향은 $GA_3$보다 ABA에 더 큰 영향을 받았다. Peroxidase 동위효소의 수는 발아, 생육이 진행됨에 따라 모든 부위에서 계속 증가하여 활성도와는 무관하게 발아 후 6일째에 가장 많이 나타났으며, 호르몬의 처리에 따른 동위효소의 변화는 동위효소의 수에 대해서 보다는 intensity에 대하여 더 큰 영향을 받은 것으로 나타났다.

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양송이 수확 후 배지로부터 식물생장촉진세균의 분리 및 생육특성 (Isolation and Characterization of Plant Growth Promoting Rhizobacteria from Waste Mushroom bed from Agaricus bisporus)

  • 정영필;경기천;장갑열;윤민호
    • 한국토양비료학회지
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    • 제44권5호
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    • pp.866-871
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    • 2011
  • An auxin-producing bacteria (3YN11-02) was isolated from waste mushroom bed from Agaricus bisporus of Chungnam Buyeo-Gun area. The strain 3YN11-02 was identified as a novel species belongs to Rahnella aquatica by a chemotaxanomic and phylogenetic nalysis. The isolate was confirmed to produce indole-3-acetic acid (IAA) which is one of auxin hormone by TLC and HPLC analysis. When the concentration of IAA was assessed by performing HPLC quantity analysis, the maximal $290mg\;L^{-1}$ of IAA detected in ether fraction extracted from the culture filtrate which was cultured in R2A broth containing 0.1% tryptophan for 24 h at $35^{\circ}C$. The molecular weight of the main peak obtained by LC-mass analysis was correspondent well to 175, that of IAA. To investigate the growth promoting effect of crop, when the culture broth of R. aquatica 3YN11-02 was infected onto water culture and seed pot of mung bean, the adventitious root induction and root growth of mung bean were 2.0 times higher than control.

육계(Cortex of Cinnamomum cassia J.Presl) 추출물로부터 부정근 형성 유도물질 분리 및 동정 (Isolation and Identification of Adventitious Root Formation Inducing Substances from Cortex of cinnamomum cassia J.Presl)

  • 여주호;백정규;박지성;김건우
    • 한국자원식물학회지
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    • 제37권1호
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    • pp.11-21
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    • 2024
  • 본 연구에서 육계 추출물로부터 cinnamyl alcohol을 분리·정제하였다. 연구 결과, H3 분획100 ㎍/mL에서 부정근 형성율이 286.6%였으며, 표준품 cinnamyl alcohol 1 mM (134.2 ㎍/mL)의 형성율이 290%로 유사한 결과를 나타내었다. 따라서 육계 methanol 추출물에 함유되어있는 cinnamyl alcohol이 녹두의 부정근 형성을 유도하는 주된 화합물일 것으로 판단된다. 육계에서 동정한 cinnamyl alcohol은 현재 식품첨가제 및 향료로써 사용되고 있으므로 안전성이 이미 보장되어 있으며, 한약재로써 사용된 육계 잔류물을 활용하여 부정근 형성 유도물질을 추출한다면비용적인 측면에서 또한 긍정적인 요인으로 작용할 것으로 판단된다. 따라서 육계는 유기농 농자재로써 충분히 사용 가능할 뿐만 아니라 신규 천연 식물생장조절제의 개발용 소재로서도 활용 가능할 것으로 사료된다.

김치용 천연 pH 조정제 연구 (Studies on the Natural pH Adjusters for Kimchi)

  • 장경숙
    • 한국식품영양과학회지
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    • 제18권3호
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    • pp.321-327
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    • 1989
  • 김치의 산패를 지연시킬 수 있는 천연 pH 조정제를 찾아내기 위하여 김치에 첨가 가능한 한방 및 식재료 72종의 1% 물추출액에 대하여 산에 대한 완충능을 조사하였다. 한방재료중에서 완충능이 100이상으로 나타난 것은 감초 150, 방풍 142, 등 14종이었다. 한방재료들은 50%씩 상호혼합한 것중 완충능이 150이상으로 높게 나타난 것은 소엽+감국, 소엽+산약, 소엽+갈근, 감국+갈근, 택사+갈근, 택사+일당귀, 산약+갈근이었다. 버섯류, 두류 및 채소류 중에서 완충능이 높게 나타난 것은 녹두 207, 대두 160, 완두 192, 느타리 225, 쑥갓 200, 비름 175, 깻잎 200, parsley 202, 피망 257 등이었으며, 이들을 상호혼합처리한 결과 두류상호간의 혼합을 제외하고는 완충능이 향상되었는데 특히 느타리+쑥갓 262, 느타리+parsley 255, 녹두+쑥갓 247, 대두+느타리 245, 대두+쑥갓 247, 대두+parsley 250으로 매우 높았다. 완충능이 높게 나타난 천연 pH 조정제를 무우즙액에 혼합한 결과 완충능에서 효과가 인정되는 것은 녹두+완두 272, 녹두+대두 282, 느타리+parsley 302, 쑥갓+비름 300, 쑥갓+깻잎 277, 비름+피망 300, 비름+parsley 280 등 이었으며 배추즙액에서 효과가 인정된 것은 완두 202, 느타리 202, 쑥갓 207, 느타리+parsley 205 등이었다.

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관상식물 삽목발근에 있어서 NAA, IBA 및 Ethychlozate의 발근촉진효과와 그 생리학적연구 (The Promotive Effect of NAA, IBA and Ethychlozate on Rooting Cuttings of Certain Ornamental Plants and Some Physiological Studies.)

  • 정해준;곽병화
    • 자연과학논문집
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    • 제1권
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    • pp.115-198
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    • 1987
  • The present studies were undertaken to elucidate the influence of auxins, auxin-like substance-ethychlozate ("Figaron"),and pH and sort of rooting media on rooted propagation of certainornamental woody plant cuttings, and to see possible changes in internal compositions characterizing after root-promoting treatment as the cutting stage proceeded. The experimental check-up srevealed and summarized as seen in the following;I. Effect of three different auxin treatments on rooting cuttings: 1) Promotive influence of auxin varied according to different concentration levels, hours of dipping treatment of the auxins, and kind of plants. The greatest effect was obtained for Forsythia ksreana with NAA and IBA, for Ligustrurn obtusifolium var. variegatum with NAA and ethychlozate, for Hydrangea macrophylla, Magnolia kobus, and Magnolia liliflora with NAA, lBA and ethychlozate also. The most effective level of the promotive agents was found 200mg/l for NAA, 1000mg/l for IBA, and 200mg/l for ethychlozate. For Weigela florida and Gardenia jasminoides, range of the most effective level was shown relatively wide spread. 2) NAA was more effective at its optimal level of the rooting agent than ethychiozate for Weigela florida, Viburnum awabuki, Forsythia koreana, Acer palmatum 'Nomura', Bouga invillea glabra, Elaeagnus umbellata, Prunus tomentosa, Ligustrum obtusifolium, Pyracantha coccinea, Cestrum noctu rnum, Hydrangea macrophylla, Codiaeum variegatum, Rhododen dron lateritium, and Ilex crenata var. macrophylla, and yet ethychlozate was found either as equally as effective or more so than NAA for Zebrina pendula, Hibiscus syriacus, Fatshedera lizei, Schefflera arboricola, Campsis grandiflo ra, Ixora chinensis, Euonymus japonica, and Magnolia liliflora. On the contrary, no the auxin effect was noted with Lagerstroemia indica, Trachelospermum asiaticum, and Syringa vulgaris. This probably indicates that these species are genetically different for the auxin response.II. Effect of different pH and sorts of cutting media on rooting cuttings: 1) Bougainvillea showed best in rooting for the number and dry weight at pH 6.5, more with ethychlozate than NAA, while Ligustrum did at pH 5.0 more with NAA than ethychlozate. pH 4.0 medium resulted in the best rooting for Rhododendron with NAA, more than ethychlozate. 2) Use of cutting medium with peat: perlite: vermiculite = 1:1:1 showed to give the greatest rooting percent and dry weight, apart from considering the number of roots. This apparently meant the fact that cutting medium has more to do with root growth than root differentiation. Rhododendron yet showed results with cutting media that use of peat: perlite = 2:1 mixed is more effective on rooting than using peat alone.III. Effect of auxinic treatments on rooting cuttings and change in some cutting compositions: 1) Under the climatic conditions of July having temperature $26.3\pm$$2.4^{\circ}C$for cutting bed, new roots of Magnolia started to show up generally 20 days after the cutting was made, whereas Cestrum did much earlier than that, namely 14 days after. 2) Although total carbohydrate content of Magnolia cuttings showed no marked change without auxin treatment, it did so with the treatment, especially 30 days after the start of cutting. Cestrum cuttings demonstrated a gradual in crease in total carbohydrate content as rooting took place, and the content became reduced more with auxin than with out, just about when rooting proceeded to 14 days after the start of cutting. 3) Magnolia generally showed an increase in total nitrogen content as rooting proceeded more, and Cestrum showed a decrease in total nitrogen of cuttings. The auxin treatment exhibited no pertinent relation with change in plant nitro gen when rooting is promoted with auxin treatment. 4) An abrupt drop of total sugar and reducing sugar was noticed as Magnolia rooting started, and this reduction was parti cularly outstanding with auxin treatment. Starch content also was decreased in the later stage of cutting with auxin treatment, and was rather increased without auxin. Although sugar content soon increased as cutting started with auxin treatment in the case of Cestrum, it became reduced after rooting took place. 5) Total phenol content increased with rooting, and this was especially true when rooting started. This increase was reversed somehow regardless of auxin treatment. A decrease in phenol of Magnolia was found more striking with auxin than without in the later stage of the cutting period. 6)Avena coleoptile test for auxin-like substances presented the physiologically active factor is more in easy-to-root Magnolia liliflora than hard-to-root Magnolia kobus, and the activity of auxin-like substances was much increased with auxin treatment. The increase in the growth promoting substances was markedly pronounced when rooting just started. The active growth substances decreased in the later stage of cutting, and certain inhibitory substances started appearing. Cestrum also showed physiologically similar growth promoting substances accompanying auxin-like active substances if auxin is treated, and some strong inhibitory substances seemed to appear in the later stage of cutting. 7) Mung-bean-rooting test indicated biologically that endogenous growth substances in Magnolia all promoted mung-bean rooting, and activity of the growth substances apparently stimulated mung-bean rooting with auxin more than without. Here auxin treatment seemed to give a rise to an increased activity of endogenous growth substances in cuttings. This activity was found much greater with either NAA or IBA than ethychlozate, and showed its peak of the activity when rooting first started taking place. Certain inhibitory substances for Avena coleoptile growth strongly promoted mung-bean rooting, and it was also much like in the case of Cestrum.

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