• Journal of Plant Biotechnology
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• 제30권4호
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• pp.381-385
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• 2003

지리오가피의 체세포배로부터 식물체재생을 위한 효율적인 방법을 개발하였다. 체세포배의 형태형성에 미치는 식물생장 조절물질의 영향을 조사하고자, 접합자 배 유래의 배발생 세포를 MS배지에 현탁배양하고, 이로부터 얻어진 자엽시기의 배를 배양접시에서 건조처리 (0∼72 h)한 후, NAA, BA, GA$_3$(0∼0.5mg/L)가 첨가된 MS배지에 배양하였다. 그 결과, 체세포배를 72시간 동안 배양접시에서 건조처리 후에 0.5mg/L NAA, 0.5mg/L BA 또는 0.5mg/L NAA, 0.5mg/L BA 0.5mg/L GA$_3$가 첨가된 MS배지에 6주간 배양하였을 때, multiple shoot가 100%로서 가장 높게 유도됨을 관찰 할 수 있었다. 가장 높은 식물체의 재생율 (29.1%)은 체세포배를 72시간 건조처리 후에,0.1mg/L NAA, 0.1mg/L BA그리고 0.1mg/L GA$_3$가 첨가된 MS배지에 6주간 이식한 후, 이를 다시 1.0mg/L GA$_3$가 첨가된 1/3MS배지에 6주간 배양하였을 때 이루어졌다. 유식물체를 vermiculite와 sand가 1:1로 첨가된 plastic pot 이식하였을 때 생존율은 약 70%로서 나타났다 이상의 결과는 지리오가피의 계량생산에 활용될 수 있을 것으로 기대된다.

• Journal of Forest and Environmental Science
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• 제25권2호
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• pp.119-126
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• 2009

An efficient in vitro plant regeneration system was developed through shoot proliferation from axillary buds of Tectona grandis (L.f), APNBV-1 (Andhra Pradesh North Badrachalam Venkatapuram-1) clone. Multiple shoots of high quality were produced in vitro from axillary bud explants. An average of 4.39 shoots/explant were obtained on Murashige and Skoog's (MS) medium supplemented with plant growth regulators (PGRs) benzyl amino purine (BA), kinetin (KN), indole acetic acid (IAA), gibberillic acid ($GA_3$), growth adjuvants casein hydrolysate (CH), adenine sulphate (Ads) and antioxidants ascorbic acid, polyvinyl pyrrollidine (PVP). Eighty five percent of rooting was observed in ex vitro rooting media containing IBA and vermiculite. In ex vitro rooting, single shoots with 2 to 3 nodes were subjected to IBA of different concentrations at different periods of time intervals. Direct rooting in vermiculite at 500 ppm concentration of IBA resulted in 4.3 number of roots with 2 cm length. Minimum response of rooting and length of roots were recorded at 100 ppm concentration of IBA. Planlets were transferred to plastic bags for short acclimatization stage in green house where they survived at 95%.

• Journal of Plant Biotechnology
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• 제31권1호
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• pp.61-65
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• 2004

본 실험은 Alocasia cadieri Chantrier를 기내배양하여 일시에 균일한 식물체를 대량생산하기 위하여 실시하였다. Alocasia의 경정을 TDZ 0.1mg/L가 첨가된 배지에 배양하였을 때 신초증식이 가장 높았으며, 형성된 신초에서 신초의 증식은 TDZ과 NAA가 각각 0.5mg/L첨가된 배지에서 효과적이었다. 형성된 신초의 발근은 IBA또는 NAA가 첨가된 배지보다는 활성탄 2.0g/L가 첨가된 배지에서 양호하였으며, 소식물체의 순화는 perlite : vermiculite가 1:1로 혼합된 용토 또는 vermiculite가 적합하였다.

• Journal of Biosystems Engineering
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• 제19권1호
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• pp.17-21
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• 1994

This reasearch was performed to provide the fundamental intonation for the mechanization of Korean pine cone harvest when the shoot shearing method is adopted. Shear force and stress of pine cone shoots were measured and analyzed for this purpose. Samples are selected along their harvesting time and tested in 17 levels of shoot diameter from 10 to 26mm with 1mm increment. 1) Shear force-deformation characteristics showed that shoot reached its rupture point after 2 to 4 of bio-yield points. It was supposed that these multiple bio-yield points were caused by the discrete compression of wood parts which are composed of water, nutrient, resin, etc. 2) Required shear force to shear shoot was proportional to the square of shoot diamter, however, shear force for shoots of early harvesting time(Aug. 31) was proportional to the shoot diameter. Variance of shear force was increased as the harvesting time was delayed. Shear forces were distributed from 468N(Aug. 31, 12mm dia) to 4153N(Aug. 31, 26mm dia) disregarding the sampling date. 3) The average shear stresses by sampling dates were 744,822, and 883N/m2, respectively, and for the earlier shoot samples shear stress was quite smaller than the others. Shear stress was proportional to shoot diameter squared, and the effect of shoot diameter on the shear stress was decreased as harvesting time was delayed.

• Journal of Plant Biotechnology
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• 제5권4호
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• pp.239-244
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• 2003

A protocol has been developed for rapid large scale clonal propagation of an aromatic endangered medicinal plant, Hemidesmus indicus R. Br. with the elimination of the problems such as premature leaf fall and callus formation during caulogenesis and rhizogenesis. Multiple shoots were induced from shoot tip and nodal explants on Murashige and Skoog (MS) medium supplemented with 1 mg/L Benzylaminopurine (BAP) and 0.5 mg/L Napthaleneaceticacid (NAA). Addition of 15 mg/L adenine sulphate to the above medium checked leaf abscission completely, reduced the time required for caulogenesis and restored morphogenetic potential after several subcultures. The in vitro grown propagules were rooted in 1/2 MS medium supplemented with 2 mg/L Indolebutyric acid (IBA) +1 mg/L NAA and sucrose 0.7% (w/v). Addition of charcoal at 100 mg/L to the rooting medium quickened root initiation with a complete check on callus formation. The effect of sucrose concentration on both caulogenesis and rhizogenesis was also studied. The resultant plantlets were acclimatized and grown in fields where ninety eight percent of the rooted shoots survived and grew normally. The estimation of the secondary metabolite content in the shoots of the regenerated plant and the mother plant indicated that the concentration of the three secondary metabolites lupeol, vanillin and rutin was similar.

• Journal of Plant Biotechnology
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• 제33권4호
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• pp.249-255
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• 2006

Effects of ethylene inhibitors like silver nitrate $(AgNO_3)$, cobalt chloride $(CoCl_2)$ and Salicylic acid (SA) on multiple shoot induction and their impact on ethylene production using embryonal cotyledon cultures of Cucumis sativus L. were examined. The optimum concentration of $AgNO_3\;(40{\mu}M),\;CoCl_2\;(20{\mu}M)\;and\;SA\;(20{\mu}M)$, separately, induced maximum number of shoots on Murashige and Skoog's (MS) medium supplemented optimally with $4.44{\mu}M$ BA and $0.25{\mu}M$ NAA. Among the three ethylene inhibitors tested, $AgNO_3$ produced maximum number of shoots when compared to $CoCl_2$ and SA Ethylene production was monitored in all the treatments with $AgNO_3/CoCl_2/SA$ and it was observed that the treatment with $AgNO_3$ alone showed increase in ethylene production when compared to $CoCl_2$ and SA Even though ethylene concentration was the highest in $AgNO_3$ treated explants, maximum number of shoots was obtained.

• Journal of Plant Biotechnology
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• 제7권3호
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• pp.155-160
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• 2005

Haloxylon recurvum (Locally known as Khar) is drought and salt tolerant plant of Thar Desert. This plant is a major biomass producer and has economic and ecological importance for the region. There is need for study on biology, propagation and genetic improvement for utilization of this plant for reclamation of saline soils. We report here on in vitro propagation of Haloxylon recurvum (Moq.) using nodal explant. Secretion of phenolic compound from explants was a major constraint for establishment of culture. This was checked by thorough washing and quick transfer of explant on fresh culture medium. Juvenile nodal explant with leaves was found suitable for culture establishment. Benzy-ladenine($4.0\;{\mu}M$) incorporated in Murashige and Skoog (MS) medium with additives (50 mg/L ascorbic acid and 25 mg/L each of adenine sulphate, arginine and citric acid) induced multiple shoots from nodal explant. Addition of $1.0\;{\mu}M$ naphthalene acetic acid (NAA) in combination with $4.0\;{\mu}M$ BAP improved the growth of axillary shoots. Further shoot amplification was achieved by repeated subculture of mother explants on fresh medium. Forty percent of the micropropagated shoots rooted on half-strength MS medium with $4.0\;{\mu}M$ indolebutyric acid (IBA) and 100 mg/L activated charcoal, at $28{\pm}2^{\circ}C$ and $60\%$ RH. Sixty percent of these plantlets were hardened in green house.

• 한국작물학회지
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• 제37권5호
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• pp.419-424
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• 1992

콩에서의 조직배양 가능성을 알아보고자 콩 유묘를 기내에서 배양시킨 뒤, 여러 부위를 채취하여 각기 다른 배지 치상한 결과는 다음과 같다. 1. 줄기정단조직 배양에서는 대부분 shoot가 분화되어 완전한 식물체를 얻을 수 있었다. 2. 자엽절 조직배양에서는 multiple shoots가 나타나 대량증식에 유용했으나 배지에 따른 영향이 컸다. 3. 배축 및 상배축 조직배양에서는 callus만 분화되엇다. 4. 엽조직배양에서는 callus 분화 후 계대배양했을 때 shoot가 분화되었으나 완전한 식물체를 얻기는 힘들었다.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2002년도 제9차 국제심포지움 및 추계정기학술발표회
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• pp.6-7
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• 2002

The goal of this research was to develop the effectiveness of in vitro culture method for A. formosanus and study the environment in vitro conditions affecting on growth. The first series of experiments were examined to investigate the response of three different basal media, MS (Murashige and Skoog, 1962), Knudson (KC; Knudson, 1946) and modified hyponex on growth and multiplication during in vitro culture. Multiple shoot proliferation was induced in shoot tip explants on Hyponex (H3) media supplemented with BA (1 mg1$^{-1}$) or TDZ (1-2 mg1$^{-1}$). Addition of activated charcoal (1%) to the TDZ containing medium promoted rapid shoot tip proliferation (11.1 shoots per explant) but the same medium had an opposite effect resulting in poor proliferation in the nodal explants. However, the regenerated shoots had slow growth rate and failed to elongate. This problem was overcome by transferring the shoot clumps to a hormone free H3 media supplemented with 2% sucrose and 0.5% activated charcoal. Using bioreactor culture for scaling up was also shown the best way for multiple shoot induction and growth of this plant.(중략)

• 식물조직배양학회지
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• 제21권3호
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• pp.145-150
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• 1994

본 연구는 감귤 품종인 '삼보감'과 '병귤'에서 3종류의 싸이토키닌(BA, 2iP, kinetin)과 그들의 농도(0, 0.1, 0.5, 1.0, 2.0mg/L)가 마디배양에 의한 대량 증식에 미치는 영향을 구명하고자 수행되었다. 마디의 절편체는 기내 발아된 두 품종의 유묘에서 얻어졌다. 두 품종을 비교하면 신초의 수는 '병귤'에서보다 '삼보감'에서 더 많이 형성되었다. 본 실험에서 사용된 3종류의 싸이토키닌 중에 고체 MS 기본배지에 첨가된 BA가 신초의 대량 생산에 가장 효과적인 촉진제였으며 그 적정농도는 1.0 mg/L인 것으로 나타났다. 줄기의 신장 뿌리의 형성은 싸이토키닌의 종른에 관계없이 싸이토 키닌의 농도가 증가함에 따라 억제되었다. 삼보감'에서는BA 1.0 mg/L에서 가장 탄은 신초 및 엽수를 형성한 반면 '병귤에서는 어떠한 싸이토키닌의 종류나 농도도 본 실험에서 신초 및 뿌리 등의 형성에 커다란 영향이 없었다.