• 미생물학회지
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• 제49권2호
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• pp.146-149
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• 2013

비브리오균은 해양 및 하구 환경에서 상재하는 균으로 그람 음성 간균, 호염성, 비포자형성의 특징을 가지고 있다. 질병과 관련된 균주로는 Vibrio parahaemolyticus, Vibrio vulnificus, Vibrio cholera 등이 있다. 매년 어패류의 섭취 등을 통해 식중독 사고가 빈번하게 발생하고 있으며, 이에 본 연구에서는 서해안 6곳에서 패류를 채취하여 비브리오균의 분포도를 모니터링하고, 이들의 항생제 내성을 분석하였다. 총 120건의 패류를 TCBS 평판배지와 API 20E kit 기법으로 동정하였을 때 23.3%의 시료에서 비브리오균이 검출되었다. 이들 분리균주들의 항생제 내성을 16종의 항생제에 대해 분석한 결과 82.1%가 각각 vancomycin과 ampicillin에 대해 내성을 보였고, rifampin (71.4%)과 cephalothin (53.6%)에 대한 내성비율도 다른 항생제들에 비해 상대적으로 높은 것으로 나타났다. 분리균주들의 항생제 감수성은 chloramphenicol (92.9%), sulfamethoxazole/trimethoprim (92.9%)과 tetracycline (96.4%)에 대해 가장 높았다. 분리균주들의 약 71.4%가 vancomycin과 ampicillin을 포함하여 3개 이상의 항생제에 대해 다중내성을 보였다.

• Current Research on Agriculture and Life Sciences
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• 제18권
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• pp.9-17
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• 2000

풋마름병과 역병에 복합저항성인 계통을 육성하기 위한 노력으로서 앞서 역병 저항성으로 육성한 계통(16-2-3-2 = 역병 저항성 '칼미초', 19-1-3-7-1-1, 19-2-4-5-3-2 = 역병 저항성 '수비초', 김 등, 1996)과 풋마름병 저항성 계통(KC350 = MC 4, KC353 = PBC631)을 교배하여 육성한 $F_5$와 $BC_1F_4$, $F_6$와 $BC_1F_5$ 세대에 대해 1999년과 2000년에 각각 풋마름병과 역병에 대한 복합저항성을 검정하였다. 풋마름병과 역병에 복합 저항성을 보인 개체를 선발하여 다음 세대의 종자를 채종하였다.

• 한국수산과학회지
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• 제49권1호
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• pp.13-19
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• 2016

A total of 423 shellfish (Oysters, Crassostrea gigas; short-necked clams, Ruditapes philippinarum and corb shells, Cyclina sinensis) were collected from the west coast of Korea, from which 277 Escherichia coli strains were isolated. The antimicrobial susceptibility patterns of isolated strains were analyzed for 22 antimicrobial agents used in Korea for clinical or veterinary therapy. The resistance of E. coli isolates to ampicillin (37.2%) was highest, followed by cephalothin (21.7%), cefazolin (19.9%), trimethoprim (15.2%). Antimicrobial resistance was present in 56.7% of E. coli isolates against at least one antimicrobial agent. Of 277 isolates, 44 (15.9%) were resistant to multiple antimicrobial agents.

• Weed & Turfgrass Science
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• 제3권2호
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• pp.110-113
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• 2014

The continuous use of acetolactate synthase (ALS) and acetyl-CoA carboxylase (ACCase) inhibitors has led to the selection of herbicide resistant barnyardgrass populations in direct-seeded rice fields of Korea. This study was conducted to identify herbicide resistant barnyardgrass biotypes and to determine the cross- and multiple-resistance of them. 25% of the population collected from Taeahn was partially resistant to ACCase inhibitors and 22% collected from Kimjae were partially resistant to ALS inhibitors. However, 8.2% of the population from both sites was resistant to ALS and ACCase inhibitors. Resistance to sulfonylurea herbicide, flazasulfuron was identified from two barnyardgrass accessions collected from both Taeahn and Kimjae. One barnyardgrass accession from both sites was resistant to ACCase inhibitor, sethoxydim. The cross-resistance to ALS inhibitors was identified at one barnyardgrass accession from Taeahn and at two accessions from Kimjae. Further, crossresistance to ACCase inhibitors was also identified at barnyardgrass accessions from Taeahn and Kimjae. Multiple-resistance to flazasulfuron and sethoxydim was determined at four barnyardgrass accessions from Taeahn and at six accessions from Kimjae. Therefore, the herbicide mixture and sequences within a growing season or the herbicide rotation with different modes of actions across growing seasons are recommended to control herbicide-resistant barnyardgrass in infested fields.

• 한국동물위생학회지
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• 제46권3호
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• pp.211-218
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• 2023

This study was carried out to investigate the prevalence and antimicrobial resistance of Salmonella serotypes in duck farms of Jeollanam-do Province, South Korea. A total of 1112 samples (breeder ducks, 286; broiler ducks, 826) were collected from 196 duck farms (breeder duck farms, 25; broiler duck farms, 171) between January 2018 and November 2019. The total prevalence of Salmonella serotypes was 45.4% (89/196) in the duck farms, with no significant difference between prevalence in breeder and broiler duck farms (48% and 45%, respectively; P>0.05). The most prevalent serotype among the 127 Salmonella isolates was Salmonella Typhimurium (20.5%) followed by Salmonella Albany (17.3%), Salmonella Hadar (15.7%), and Salmonella Enteritidis (11.8%). Maximum resistance was observed against penicillin (78.74%), followed by tetracycline (68.50%), and kanamycin (65.35%). Of the 127 isolates, 117 (92.13%) were resistant to ≥3 antimicrobials and 2 to all 18 antimicrobials. Our results demonstrate the presence of Salmonella strains and their resistance to multiple antimicrobials, thus indicating a public health concern in South Korea. The emergence of Salmonella stains that are resistant to multiple drugs highlight the need for careful use of antimicrobials in duck farms.

• 한국응용곤충학회지
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• 제26권4호
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• pp.257-259
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• 1987

131개 땅콩품종을 공시하여 녹병, 검은무늬병, Phaeoisariopsis 점무늬병에 대한 복합저항성을 조사한 결과 7개품종이 저항성이었고 11개품종은 중도저항성이었다. 각 품종들의 발병 정도와 수량에 대한 효과가 논의되었다.

• 한국해양공학회지
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• 제9권1호
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• pp.111-119
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• 1995

The elastic-plastic fracture toughness($J_{IC}$) and fracture resistance (J-R curve) of SA508-3 alloy steel used for nuclear reactor pressure vessel are investigated by using CT-type specimens. Fracture toughness tests are conducted by unloading compliance method and multiple specimen method at room temperature, -2$0^{\circ}C$ and 20$0^{\circ}C$. The apparent negative crack growth phenomenon which usually arises in partial unloading compliance test is well known. The negative crack growth phenomenon in determining J sub(IC) or J-R cure from partial unloading compliance experiments may be eliminated by the offset technique. In this study, the evaluation of $J_{IC}$ multiple specimen method recommended by the JSME gives the most reliable results by using half-size CT(similar-type) specimens.

• 한국생명과학회:학술대회논문집
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• 한국생명과학회 2007년도 제48회 학술심포지움 및 추계국제학술대회
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• pp.105.1-105.1
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• 2007

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• 미생물학회지
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• 제48권1호
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• pp.22-28
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• 2012

장염비브리오는 우리나라에서 하절기에 발생하는 식중독의 주요 원인균으로 주로 해수 및 어패류를 통해 인체에 감염된다. 최근 지구 온난화로 해수의 온도가 상승하면서 해양환경 중 세균들의 분포도가 증가하고 또 다양한 항생제에 대한 내성을 보이는 병원균들이 확산되고 있어 국민보건 및 수산양식 등의 분야에 큰 문제로 대두되고 있다. 이에 본 연구에서는 경기, 인천지역 7곳에서 해수 및 어패류 시료를 채취하여 장염비브리오균의 분포도를 모니터링하고 이들의 항생제 내성 양상을 분석하였다. 총 966건의 시료를 $CHROMagar^{TM}$ 및 TCBS 평판배지와 multiplex PCR기법으로 동정했을 때 전체 해수 시료의 61.9%, 어패류 시료의 41.8% 등 총 47.7%의 시료에서 장염비브리오균이 검출되었다. 이들 분리균주들의 항생제 내성을 13종의 항생제에 대해 분석한 결과 97.3%와 87.3%가 각각 vancomycin과 ampicillin에 대해 내성을 보였고 cephalothin (48.8%)과 rifampin (46.1%)에 대한 내성비율도 기타 다른 항생제들에 비해 상대적으로 높은 것으로 나타났다. 분리균주들의 항생제 감수성은 chloramphenicol (91.7%)과 trimethoprim-sulfamethoxazole (91.8%)에 대해 가장 높았고, gentamycin (82.3%), tobramycin (74.8%), nalidixic acid (71.6%), tetracyclin (69.4%), cefotaxime (63.0%) 등의 순서로 감수성 비율이 높았다. 분리균주들의 약 69%가 vancomycin과 ampicillin을 포함하여 3개 이상의 항생제에 대해 다중내성을 보였고 특히 11개 항생제에 대해 내성을 보이는 균주도 2개 검색되었다. 항생제 내성 장염비브리오 분리균주들의 플라스미드 프로파일을 분석한 결과 55.1%가 플라스미드를 보유하고 있었으며, 이들은 총 24가지 유형으로 분류되었으나 플라스미드 프로파일과 항생제 내성 간의 뚜렷한 상관관계는 관찰되지 않았다.

• Journal of Plant Biotechnology
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• 제43권3호
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• pp.317-331
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• 2016

Development of disease resistant plant is one of the important objectives in rice breeding programs because biotic stresses can adversely affect rice growth and yield losses. This study was conducted to identify lines with multiple-resistance genes to biotic stress among 173 hybrid rice breeding lines and germplasms using DNA-based markers. Our results showed that one hybrid rice line [IR98161-2-1-1-k1-3 (IR86409-3-1-1-1-1-1/IRBB66)] possessed 5 bacterial blight resistance genes (Xa4, xa5, Xa7, Xa13 and Xa21) while two hybrid rice lines [IR98161-2-1-1-k1-2 (IR86409-3-1-1-1-1-1/IRBB66) and 7292s (IR75589-31-27-8-33S(S1)/IR102758B)] possessed 3 bacterial blight resistance genes (Xa4, Xa7 and Xa21, and Xa3, Xa4 and xa5). Molecular survey on rice blast disease revealed that most of these lines had two different resistant genes. Only 11 lines possessed Pib, Pi-5, and Pi-ta. In addition, we further surveyed the distribution of insect resistant genes, such as Bph1, Bph18(t), and Wbph. Three hybrid breeding lines [IR98161-2-1-1-k1-3 (IR86409-3-1-1-1-1-1/IRBB66), IR98161-2-1-1-k1-2 (IR86409-3-1-1-1-1-1/IRBB66), and 7292s (IR75589-31-27-8-33S(S1) /IR102758B)] contained all three resistance genes. Finally, we obtained four hybrid rice breeding lines and germplasms [IR98161-2-1-1-k1-2 (IR86409-3-1-1-1-1-1/IRBB66), Damm-Noeub Khmau, 7290s, and 7292s (IR75589-31-27-8-33S(S1)/IR102758B)] possessing six-gene combination. They are expected to provide higher level of multiple resistance to biotic stress. This study is important for genotyping hybrid rice with resistance to diverse diseases and pests. Results obtained in this study suggest that identification of pyramided resistance genes is very important for screening hybrid rice breeding lines and germplasms accurately for disease and pest resistance. We will expand their cultivation safely through bioassays against diseases, pests, and disaster in its main export countries.