• Proceedings of the Korean Society of Plant Biotechnology Conference
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• 2002.04b
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• pp.143-143
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• 2002

• Journal of Plant Biotechnology
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• v.46 no.1
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• pp.32-36
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• 2019

Calla lilies (Zantedeschia spp.) are monocotyledonous ornamental plants which belongs to the Araceae family. After the release of elite calla cultivar, an efficient propagation system is needed for commercial use. Despite the use of conventional propagation methods such as splitting of tubers and rhizomes of calla, rapid and efficient propagation system should be developed. In order to achieve this goal, stem segments contained apical meristems derived from calla lily cultivar (cv. Gag-si) were cultured on Murashige and Skoog (MS) medium supplemented with various concentrations of cytokinin and auxin. This was aimed at inducing embryogenic calluses, shoots and multiple shoots. As a result, about 25% of induction rates of yellow embryogenic calluses were observed with MS medium containing both $0.5mg{\cdot}L^{-1}\;NAA$ and $1.5mg{\cdot}L^{-1}\;BA$ as growth regulators. In the experiments involving the regeneration from embryogenic calluses through shoot formation, MS medium supplemented with $0.5mg{\cdot}L^{-1}\;IAA$ and $2.0mg{\cdot}L^{-1}\;BA$ showed the highest rates at approximately 85 ~ 90% with regard to the formation of shoots in calla. Moreover, multiple shoots needed for rapid propagation were generated when explants were cultured on MS medium supplemented with $0.5mg{\cdot}L^{-1}\;IAA$ and $2.0mg{\cdot}L^{-1}\;BA$ with 40% of formation rate. In this study, the combination of auxin and cytokinin showed positive effects on both the induction of embryogenic calluses, the formation of shoots as well as multiple shoots in calla. The regeneration system described here can contribute to the development of breeding programs of calla in the future.

• Proceedings of the Korean Society of Plant Biotechnology Conference
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• 2002.04a
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• pp.33-40
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• 2002

We, Tong Yang Moolsan Co. Ltd. (TYM) set up the mass-production system for virus-free seed garlic via tissue culture technique. TYM's tissue culture technique is called as 'Multiple shoot propagation technique'. This technique can lead mass propagation of genetically homogeneous seed garlic in a short period because of its highly proliferation rate of in vitro shoots ($15^{10}$ /year). TYM researchers applied the technique to some selected garlic cultivars with superior characteristics and carried out field test of productivity in the inside and outside of the country for several years. According to the yearly results of field test with virus-free seed garlic, we ascertained that virus-free seed garlic can produce the highly yield increase (max. above $50\%$) and also can enhance the product quality. Consequently, we estimated that TYM's seed garlic will contribute to farmers with increase of income and can elevate the national position of garlic market in the world for its competitive power of technical and production cost.

• Journal of the Korean Institute of Intelligent Systems
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• v.14 no.5
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• pp.539-544
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• 2004

We investigate a visual object tracking algorithm based upon particle filters, namely CONDENSATION, in order to combine multiple observation models such as active contours of digitally subtracted image and the particle measurement of object color. The former is applied to matching the contour of the moving target and the latter is used to independently enhance the likelihood of tracking a particular color of the object. Particle filters are more efficient than any other tracking algorithms because the tracking mechanism follows Bayesian inference rule of conditional probability propagation. In the experimental results, it is demonstrated that the suggested contour tracking particle filters prove to be robust in the cluttered environment of robot vision.

• JSTS:Journal of Semiconductor Technology and Science
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• v.14 no.6
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• pp.824-831
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• 2014

In this study, structural variations and overlay errors caused by multiple patterning lithography techniques to print narrow parallel metal interconnects are investigated. Resistance and capacitance parasitic of the six lines of parallel interconnects printed by double patterning lithography (DPL) and triple patterning lithography (TPL) are extracted from a field solver. Wide parameter variations both in DPL and TPL processes are analyzed to determine the impact on signal propagation. Simulations of 10% parameter variations in metal lines show delay variations up to 20% and 30% in DPL and TPL, respectively. Monte Carlo statistical analysis shows that the TPL process results in 21% larger standard variation in delay than the DPL process. Crosstalk simulations are conducted to analyze the dependency on the conditions of the neighboring wires. As expected, opposite signal transitions in the neighboring wires significantly degrade the speed of signal propagation, and the impact becomes larger in the C-worst metals patterned by the TPL process compared to those patterned by the DPL process. As a result, both DPL and TPL result in large variations in parasitic and delay. Therefore, an accurate understanding of variations in the interconnect parameters by multiple patterning lithography and adding proper margins in the circuit designs is necessary.

• Smart Structures and Systems
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• v.30 no.4
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• pp.397-410
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• 2022

It is essential to monitor the working conditions of bolt-connected joints, which are widely used in various kinds of steel structures. The looseness of bolts may directly affect the stability and safety of the entire structure. In this study, a guided wave-based method for bolt looseness detection and localization is presented for a joint structure with multiple bolts. SH waves generated and received by a small number (two pairs) of magnetostrictive transducers were used. The bolt looseness index was proposed based on the changes in the reconstructed responses excited by the time reversal signals of the measured unit impulse responses. The damage locations and local damage severities were estimated using the damage indices from several wave propagation paths. The back propagation neural network (BPNN) technique was employed to identify the local damages. Numerical and experimental studies were conducted on a lap joint with eight bolts. The results show that the total damage severity can be successfully detected under the effect of external force and measurement noise. The local damage severity can be estimated reasonably for the experimental data using the BPNN constructed by the training patterns generated from the finite element simulations.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.41 no.6
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• pp.704-709
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• 1996

This experiment was carried out to establish the system of mass propagation through tissue culture using sucker and stem in Ramie. The sterilization for tissue culture of Ramie was the better treatment of 2% NaClO for 20 minute into ultrasonic cleaner than the others, and so rate of contamination was 3.3%, and it was able to produce 96% healthy plant. The effect of growth regulator was superior to mixed treatment of 0.02mg/$\ell$ NAA, 1.5mg/$\ell$ BA, 0.lmgmg/$\ell$ GA$_3$, which it was not formed callus and but produced 96% healthy plant. The effect of propagation was higher in culturing of the stem node than the sucker in cultural part, local variety than improved ones. The effect of acclimatization was superior to pretreatment of 30 minute after soaking in 100ppm NAA, transplanting on bed soil which mixed to ratio of vermiculite : soil : sand =1 : 2 : 1, the transplanted plants were grown all normal.

• Journal of the Korean Society for Aeronautical & Space Sciences
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• v.31 no.9
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• pp.26-31
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• 2003

Aging aircraft accumulates widespread fatigue damage commonly referred to as multiple site damage(MSO). For ductile material such as 2024-T3 aluminum, MSO may lower the service life below that which is predicted by conventional fracture mechanics. The present paper is concerned with the fatigue life extension by pre-indentation technique for thin 2024-T3 aluminum plate to decelerate the crack propagation rate in the panels with MSO. The panel with fastener holes can be simply modelled by Hole/Slot type Middle-Tension specimen. Results of fatigue testing show significantly improving failure cycles from 10 to 40 times. This retardation effect is decreased by increasing the loading level in the constant amplitude loading. In the sense of retardation mechanism, the crack propagation rate is gradually attenuated by entering the indentation mark and maintains at the lowest value for a long period after the edge of crack passes the center of indentation area.

• Journal of the Korean Society of Safety
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• v.26 no.2
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• pp.32-35
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• 2011

Experimental studies were carried out in an explosion chamber to investigate the influences of multiple cylinder obstacles on local flame propagation. The chamber dimension is 235 mm in height with a $1,000{\times}950\;mm^2$ rectangular cross section and a large vent area of $1,000{\times}320\;mm^2$. Multiple cylinder bars with obstruction ratio of 30% were used. In order to examine the interaction between the propagating flames and the obstacles, temporally resolved flame front images were recorded by a high speed video camera. The propagation behaviour of local flame fronts around the left obstacle was analyzed in terms of two different methods such as the incremental burnt area divided by the flame front length and the average of the local propagation velocity determined at each point along the flame front. It was found that two methods give good consistency.

• Structural Engineering and Mechanics
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• v.66 no.3
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• pp.343-351
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• 2018

Brazilian disc test is one of the most widely used experiments in the literature of geo-mechanics. In this work, the pre-holed concrete Brazilian disc specimens are numerically modelled by a two-dimensional discrete element approach. The cracks initiations, propagations and coalescences in the numerically simulated Brazilian discs (each containing a single cylindrical hole and or multiple holes) are studied. The pre-holed Brazilian discs are numerically tested under Brazilian test conditions. The single-holed Brazilian discs with different ratios of the diameter of the holes to that of the disc radius are modelled first. The breakage load in the ring type disc specimens containing an internal hole with varying diameters is measured and the crack propagation mechanism around the wall of the ring is investigated. The crack propagation and coalescence mechanisms are also studied for the case of multi-holes' concrete Brazilian discs. The numerical and experimental results show that the breaking mechanism of the pre-holed disc specimens is mainly due to the initiation of the radially induced tensile cracks which are growth from the surface of the central hole. Radially cracks propagated toward the direction of diametrical loading. It has been observed that for the case of disc specimens with multiple holes under diametrical compressive loading, the breaking process of the modelled specimens may occur due to the simultaneous cracks propagation and cracks coalescence phenomena. These results also show that as the hole diameter and the number of the holes increases both the failure stress and the crack initiation stress decreases. The experimental results already exist in the literature are quit agree with the proposed numerical simulation results which validates this simulation procedure.