• The Plant Pathology Journal
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• 제21권4호
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• pp.334-342
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• 2005

The prototype Chlorella virus PBCV-l encodes 11 tRNA genes and over 350 protein-encoding genes in its 330 kbp genome. Initial attempts to overexpress the recombinant A189/192R protein, a putative virus attachment protein, in E. coli strain BL21(DE3) SI were unsuccessful, and multiple protein bands were detected on Western blots. However, the full-length A189/192R recombinant protein or fragments derived from it were detected when they were expressed in E. coli BL21 CodonPlus (DE3) RIL, which contains extra tRNAs. Codon usage analysis of the a189/192r gene showed highly biased usage of the AGA and AVA codons compared to genes encoded by E. coli and Chlorella. In addition, there were biases of XXA/U($56\%$) and XXG/ C($44\%$) in the codons recognized by the viral tRNAs, which correspond to the codon usage bias in the PBCV-1 genome of XXA/U ($63\%$) over those ending in XXC/G ($37\%$). Analysis of the codon usage in the major capsid protein and DNA polymerase showed preferential usage of codons that can be recognized by the viral tRNAs. The Asn (AAC) and Lys (AAG) codons whose corresponding tRNA genes are duplicated in the tRNA gene cluster were the most abundant (i.e., preferred) codons in these two proteins. The tRNA genes encoded in the PBCV-l genome seem to play a very important role during the synthesis of viral proteins through supplementing the tRNAs that are frequently used in viral proteins, but are rare in the host cells. In addition, these tRNAs would help the virus to adapt to a wide range of hosts by providing tRNAs that are rare in the host cells.

• 국제지역연구
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• 제14권3호
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• pp.131-154
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• 2010

본 논문은 브라질 현지에 진출하여 직접 경영활동을 수행하고 있는 한국기업들이 직면하고 있는 정치적 환경요인이 무엇인가를 규명하고 이에 어떠한 대응책을 마련하여 경영활동을 전개하고 있는 가를 밝히기 위한 것이라 할 수 있다. 기업의 경영활동에 영향을 미치는 일반 환경요인과 산업 환경요인 중에서 일반 환경요인으로 연구의 대상을 제한하였는데 그 이유는 기업이 오히려 일반 환경에 대한 분석을 체계적으로 실행하고 있지 않다는 지적이 있기 때문이다. 그리고 본 연구에서는 일반 환경요인 중에서 정치적 환경요인만을 분석에 대상에서 포함시켰는데 기업의 현지경영활동에 가장 직접적이고 가시적인 영향력을 가장 강력하게 행사하고 있다는 판단 때문이다. 선행연구를 통해 문헌분석을 실행하였고 이를 토대로 연구가설을 설립한 후 이를 검증하기 위한 실증분석을 실시하였다. 분석결과, 현지의 행정 관료의 비합리적 행태들과 관련 법 시스템의 임의적인 적용환경에 대응하기 위하여 합리적인 적응과 인간관계의 강화 등의 현지경영전략을 실행하고 있는 것으로 나타났다. 활용한 통계분석 프로그램은 기술통계를 위해서는 SPSS 12.0 그리고 추리통계에는 AMOS 12.0 등이다.

• Earthquakes and Structures
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• 제16권2호
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• pp.185-196
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• 2019

One of the shortcomings of seismic bridge design codes is the lack of clarity in defining the role of different seismic isolation systems with linear or nonlinear behavior in terms of R-factor. For example, based on AASHTO guide specifications for seismic isolation design, R-factor for all substructure elements of isolated bridges should be half of those expressed in the AASHTO standard specifications for highway bridges (i.e., R=3 for single columns and R=5 for multiple column bent) but not less than 1.50. However, no distinction is made between two commonly used types of seismic isolation devices, i.e., elastomeric rubber bearing (ERB) with linear behavior, and lead rubber bearing (LRB) with nonlinear behavior. In this paper, five existing bridges located in Iran with two types of deck-pier connection including ERB and LRB isolators, and two bridge models with monolithic deck-pier connection are developed and their R-factor values are assessed based on the Uang's method. The average R-factors for the bridges with ERB isolators are calculated as 3.89 and 4.91 in the longitudinal and transverse directions, respectively, which are not in consonance with the AASHTO guide specifications for seismic isolation design (i.e., R=3/2=1.5 for the longitudinal direction and R=5/2=2.5 for the transverse direction). This is a clear indicator that the code-prescribed R-factors are conservative for typical bridges with ERB isolators. Also for the bridges with LRB isolators, the average computed R-factors equal 1.652 and 2.232 in the longitudinal and transverse directions, respectively, which are in a good agreement with the code-specified R-factor values. Moreover, in the bridges with monolithic deck-pier connection, the average R-factor in the longitudinal direction is obtained as 2.92 which is close to the specified R-factor in the bridge design codes (i.e., 3), and in the transverse direction is obtained as 2.41 which is about half of the corresponding R-factor value in the specifications (i.e., 5).

• 디지털융복합연구
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• 제22권2호
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• pp.11-20
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• 2024

본 연구는 전환장애 증상 중 우울감을 경험하는 60대 여성 22명을 대상으로 2021년 1월26일부터 2월9일까지 실험집단 대상의 연구를 시행하였다. 60대 노년여성의 우울감 감소와 신체증상 완화를 위해 통합심리학적 치료 프로그램인 집단상담 프로그램을 개발하고, 연구 대상자들의 사전-사후 변화를 비교 분석하여 효과성을 검증하고자 하였다. 이를 위하여 22명의 60대 여성을 대상으로 한국형 노인 우울 검사(K-GDS), 증상해석 질문지(SIQ), Hamilton 우울 평가척도 설문지, PHQ-9 및 대인관계 척도(RCS)를 실시하였다. 이후 사전-사후 대응 표본 t 검정, 상관분석과 다중 회귀 분석을 수행하였다. 연구 결과, 본 연구가 개발한 집단상담 프로그램을 통하여 60대 여성의 우울감 감소, 신체증상 완화, 부정적 정서 및 부정적 신념 감소, 대인관계에 긍정적인 변화가 나타나 그 효과를 실증적으로 입증하였다. 마지막으로, 본 연구의 의의와 한계를 논의하였다.

• 한국수산과학회지
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• 제57권3호
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• pp.239-252
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• 2024

Recombination activating genes (RAGs) play a crucial role in initiating V(D)J recombination, which is essential for developing adaptive immunity in vertebrates. In this study, we cloned and characterized RAG1/2 cDNA from the marine medaka Oryzias dancena (OdRAG1/2) and investigated their mRNA expression patterns during ontogenetic developmental stages. The OdRAG1 and OdRAG2 cDNA contained open reading frames (ORFs) encoding proteins containing 1,078 and 531 amino acids, respectively. Multiple sequence alignment and phylogenetic analysis revealed that OdRAG1 and OdRAG2 are highly conserved with their corresponding orthologs, featuring distinct core and non-core regions. Notably, expression analysis showed that, in contrast to other fish RAGs studied, OdRAG1/2 expression peaked at 0 days post-hatching (DPH). Additionally, for the expression of T and B cell differentiation markers, CD3γ and CD20, also peaked at 0 DPH. Collectively, adaptive immunity in O. dancena potentially begins during embryonic development, which is critical for V(D)J recombination and essential immune component development, suggesting the early ontogenetic stage interactions between innate and adaptive immunity.

• Asian Pacific Journal of Cancer Prevention
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• 제15권22호
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• pp.9679-9685
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• 2014

Background: When evaluating health-economics for cervical cancer prevention policies in Japan, it is important to use Japanese value settings. This study aimed to obtain preference-based measures (preference measures) for hypothesized health states among healthy Japanese women, and to examine differences between the EuroQol-5D (EQ-5D) and standard gamble (SG) instruments. Materials and Methods: The investigation was performed among female students at a nursing university. We used written hypothetical scenarios describing three grades of cervical intraepithelial neoplasia (CIN) and eight stages of cervical cancer, both at diagnosis and after medical intervention. Preference measures were evaluated using both EQ-5D and SG. Results: We received responses from 136 women. The mean number of respondents per stage was 24.6 (SD: 2.7). At diagnosis, average EQ-5D scores for CIN1, CIN2, CIN3, IA1, IA2, IB1, IB2, IIA, IIB, III, and IV stages were 0.84 (0.14), 0.78 (0.12), 0.73 (0.10), 0.78 (0.12), 0.72 (0.12), 0.63 (0.13), 0.64 (0.12), 0.68 (0.08), 0.62 (0.13), 0.55 (0.21), and 0.18 (0.24), respectively. Using one-way analysis of variance with the Tukey-Kramer method for multiple comparisons (each stage vs. CIN1), we found significant differences for IB1 and more advanced stages (p<0.05). After medical intervention, corresponding EQ-5D scores were 0.84 (0.12), 0.81 (0.12), 0.84 (0.12), 0.80 (0.15), 0.78 (0.11), 0.64 (0.15), 0.63 (0.15), 0.71 (0.15), 0.50 (0.17), 0.52 (0.17), 0.21 (0.28). The multiple comparisons identified significant differences for IB1 and more advanced stages, excepting IIA (p<0.05). SG evaluations were more variable and relatively higher than EQ-5D evaluations. Conclusions: We obtained preference measures for three grades of CIN1-3 and eight stages of cervical cancer. In combination with appropriate sensitivity analyses, these preference measures will provide a basis for an economic evaluation of cervical cancer prevention in Japan. We suggest that EQ-5D is appropriate for cost-utility analysis of this topic.

• 유통과학연구
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• 제13권4호
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• pp.55-64
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• 2015

Purpose - Multi-dimensional prices comprise multiple components such as monthly payments and a number of payments rather than a single lump-sum amount. According to previous studies, an increase in the number of price dimensions leads to a massive amount of cognitive stress resulting in incorrect calculation, and deterioration in the consistency of the price judgment. However, an increase only in the level of complexity of calculating multi-dimensional prices does not always result in a corresponding decrease in the accuracy of price evaluation. Since diverse variables could affect consumers' purchase-decision-making process, the results of price evaluation would be different. In this study, an empirical analysis was performed to determine how the accuracy of price evaluation varies depending on the extent of the complexity of price dimensions using product involvement and brand preference as moderating variables. Research design, data, and methodology - A survey was conducted on 260 students, and 252 effective responses were used for analysis. The data was analyzed using t-test, one-way ANOVA, and two-way ANOVA. In this study, six hypotheses were developed to examine the effect of product involvement and brand preference on consumers' evaluation effort of multi-dimensional prices. Results - As the number of price dimensions increased, accuracy of price evaluation appeared to be low in high involvement, as expected. However, it showed no differences in price evaluation effort when the level of complexity of calculating multi-dimensional prices is low. When a small number of price dimensions are presented in both cases of high and low involvement, accuracy of price evaluation is much higher in a weak brand preference. On the contrary, a strong brand preference enhances an accuracy of price evaluation only in case of low involvement when the number of price dimensions is increased. An interaction effect of product involvement and brand preference on consumers' evaluation of multi-dimensional prices did not exist irrespective of the level of complexity of calculating prices being high or low. Conclusions - When the number of price dimensions is small, consumers' effort for price evaluation shows almost no difference without the moderating effect of involvement, and a weak brand preference leads to a higher accuracy of price evaluation in an effort to make the best selection. No interaction effect of product involvement and brand preference was found except for a main effect of brand preference. When a price is composed of multiple dimensions rendering it more difficult to calculate the final price, the effort for price evaluation was expected to decrease only slightly in case of combination of high involvement and strong brand preference. This is because people have a higher purchase intentions and trust for that particular brand. However, the accuracy of price evaluation was much lower in cases of high involvement, and there was no interaction effect between product involvement and brand preference except for a main effect of involvement and brand preference, respectively.

• 한국멀티미디어학회논문지
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• 제11권9호
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• pp.1296-1301
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• 2008

초음파 영상은 초음파 펄스를 이용하여 반사파를 수신하여 진단에 필요한 영상을 구성하는데 신호가 약해 질 경우 잡음이 발생하며 미세한 명암도 차이 등에 의해 분석 과정에서 육안으로 인지하고 진단하는데 어려움이 있다. 특히 근골격계 검사를 위한 초음파 영상에서 근육 영역의 진단에 어려움을 준다. 따라서 본 논문에서는 초음파 영상에서 영상 처리 기법을 이용하여 근육 영역을 검출할 수 있는 방법을 제안한다. 초음파 영상에서의 근육 영역 검출은 피하지방층과 기타 영역 그리고 근육을 둘러싸고 있는 근막 후보 영역을 검출한 후, 위치 정보와 형태학적 특징을 이용하여 최종적으로 근막 내부 영역인 근육 영역을 검출한다. 제안된 방법의 근막 후보 영역의 검출 과정은 개선된 히스토그램 스트레칭과 Multiple 연산으로 대비차를 향상시키고 반복 이진화 기법을 적용한 후, 잡음에 의해 손실되거나 끊어진 근막 영역을 거리 및 방향 분석을 이용하여 연결한 후에 근막 후보 영역을 검출한다. 검출된 근막 후보 영역의 형태학적 특징과 위치정보를 이용하여 피하지방층과 기타 영역을 분류한 후, 최종적으로 근육 영역을 검출한다. 실제 초음파 영상을 대상으로 제안된 근육 검출 방법을 적용하여 검출된 근육 영역과 전문의가 분석한 근육 영역을 비교한 결과, 제안된 근육 검출 방법이 전문의가 육안으로 분석한 근육 영역과 근접하게 검출되어 본 논문에서 제안한 근육 영역 검출 방법이 효율적임을 확인하였다.

• 한국콘텐츠학회논문지
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• 제17권12호
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• pp.92-103
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• 2017

본 연구는 스포츠브랜드와 스포츠 스타의 개성이 브랜드 애착과 브랜드 충성도에 미치는 영향과 차이를 비교분석 하였다. 이를 위해 부산, 경상 지역의 대학교의 대학생들을 대상으로 설문조사를 실시하였으며, 총 316부를 본 연구의 유효표본으로 선정하여 분석하였다. 자료 분석방법으로는 SPSS 18.0 version을 이용하여 빈도분석, 탐색적 요인분석, 신뢰도분석, 상관관계분석, 대응표본 t-test, 다중회귀분석을 실시하였다. 연구결과, 첫째, 스포츠브랜드 개성 중 흥미, 세련, 강인 순으로 브랜드 애착에 유의한 영향을 미쳤다. 둘째, 스포츠브랜드 개성 중 흥미, 세련, 강인 순으로 행동적 충성도에 유의한 영향을 미쳤으며, 강인, 흥미, 능력 순으로 태도적 충성도에 영향을 미쳤다. 셋째, 스포츠 스타 개성 중 흥미, 신뢰, 세련 순으로 브랜드 애착에 유의한 영향을 미쳤다. 넷째, 스포츠 스타 개성 중 흥미, 신뢰, 능력 순으로 행동적, 태도적 충성도에 유의한 영향을 미쳤다. 다섯째, 스포츠브랜드 개성과 스포츠 스타 개성의 차이검증을 분석한 결과 모든 요인에서 평균값의 차이가 통계적으로 유의한 것으로 나타났다.

• Journal of Pharmaceutical Investigation
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• 제37권4호
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• pp.223-227
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• 2007

A rapid, simple and sensitive LC/MS/MS method for the determination of lercanidipine in human serum was validated and applied to the pharmacokinetic study of lercanidipine. Lercanidipine and internal standard, amlodipine, were extracted from human serum by liquid-liquid extraction with hexan-isoamyl alcohol (100: 1, v/v) and analyzed on a $Symmetry^{(R)}$ MS $C_{18}$ column with the mobile phase of acetonitrile-0.2% aqueous formic acid (70: 30, v/v). Using MS/MS with multiple reaction monitoring (MRM) mode, lercanidipine and amlodipine were detected without severe interferences from human serum matrix. Lercanidipine produced a protonated precursor ion ($[M+H]^+$) at m/z 612.3 and a corresponding product ion at m/z 280.0. Internal standard produced a protonated precursor ion ($[M+H]^+$]) at m/z 409.0 and a corresponding product ion at m/z 238.0. The ruggedness of this method was investigated using quality control (QC) samples. This method showed linear response over the concentration range of 0.05-20 ng/mL with correlation coefficient greater than 0.999. The lower limit of quantitation using 0.5 mL of serum was 0.05 ng/mL, which was sensitive enough for pharmacokinetic studies. The overall accuracy of the developed method ranged from 85.51 to 112.2% for lercanidipine with overall precision (% C.V.) being 3.56-13.1%. This method showed good ruggedness (within 15% C.V.) and was successfully applied for the analysis of lercanidipine in human serum samples for the pharmacokinetic studies, demonstrating the suitability of the method.