• Animal cells and systems
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• v.3 no.4
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• pp.407-412
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• 1999

The influence of eating habits and food type on the ultrastructural characteristics of the digestive tracts was studied under the scanning and transmission electron microscopes in two crustacean decapods (Hemigrapsus penicillatus De Haan; mud crab, Pinnotheres cyclinus Shen; symbiotic crab). The relative ratio of the length of midgut versus hindgut was 1:1 in the mud crab, but 4:1 in the symbiotic crab. Observation through the scanning electron microscope revealed that the midguts of both species have densely-arranged longitudinal mucosal folds with a smooth surface. In the hindgut of the mud crab, mucosal folds were longitudinally oriented, clusters of two to five spines were observed on the cuticular surface, and the length of the spine in the distal hindgut was longer than that in the proximal portion. In the symbiotic crab, the mucosal folds were irregulary arranged, and numerous rudimentary spinal structures were noted on the cuticular surface. Through observation of a transmission electron microscope, the epithelial cells of the midgut in both species had numerous microvilli, but the length of the microvilli was slightly longer in the mud crab than in the symbiotic crab. The central layer of the basement membrane and the muscular layer of the midgut were more developed in the mud crab than in the symbiotic crab. The thickness of the cuticular layer over the hindgut surface in the mud crab was about 4 times than that of the symbiotic crab.

• Ocean and Polar Research
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• v.31 no.3
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• pp.257-264
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• 2009

Seventeen females of the mud crab Scylla serrata, from the State of Kosrae, Micronesia, were transported to the Fisheries Resources Research Institute, Gyeongsangnam-do, in oxygen-filled plastic bags. After acclimatization to a $30^{\circ}C$ holding temperature, nine females were selected for seed production trials. Spawning was hastened using eyestalk ablations; however, this may not be required in commercialscale mud crab seed production. Primary spawning produced an average of 2.4 million hatched larvae, whereas secondary spawning produced 0.4 million. About 10 days elapsed between spawning and hatching and 30 days between hatching and crablet. Mass mortalities up to 90% were observed between stages zoea 1 and zoea 2 in every trial. The highest survival rate from zoea 1 to crablet was estimated at 0.25%. Most commercial shrimp hatcheries in Korea are equipped with almost all necessary facilities and could be converted easily to mud crab hatcheries, able to run three to four times per year using hatchery technologies developed for blue crabs and Chinese mitten crabs.

• Ocean and Polar Research
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• v.31 no.4
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• pp.319-326
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• 2009

Analysis of four microsatellite markers from Mud Crab Scylla serrata revealed that there is high level of genetic diversity within this species. Genetic diversity of S. serrata was calculated using allele diversity, observed heterozygosity, expected heterozygosity (Het-exp), polymorphic information content, gene differentiation and Nei's $D_{A}$ distance. Mean polymorphic information content value was 0.797, which reflected high level of polymorphism across the loci of S. serrata. The Palau population has the highest genetic diversity (Het-exp=0.871), while the Kosrae population has the lowest genetic diversity (Hetexp=0.806). However, the geographical genetic distance among S. serrata populations from Yab, Chuuk, Pohnpei, Kosrae, and Palau were low (0.2009${\sim}$0.3350). These results suggest that despite their wide distribution, S. serrata are no different in geographical genetic diversity within the five sampled locations.

• The Korean Journal of Zoology
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• v.34 no.3
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• pp.289-299
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• 1991

Two new species of the genus Hemicyclops, H. 형omsoensis n. sp. and H. soxatilis n. sp., taken from crab burrows on each of mud flat and rocky shore, are described from the Yellow Sea coast of southwest Korea.

• Microbiology and Biotechnology Letters
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• v.49 no.3
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• pp.432-439
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• 2021

Microbial community plays important roles in the culture environment of mud crab Scylla serrata. One of the environmental management efforts for the cultivation of S.serrata is by stabilizing microorganisms involved in nitrogen cycle process. The availability of dissolved inorganic nitrogen in its culture environment under a recirculating system closely relates to the nitrogen cycle, which involves both anaerobic and aerobic bacterial activities. Anaerobically, there are two major nitrogen compound degradation processes, i.e., denitrification and dissimilatory nitrate reduction to ammonium (DNRA). This study aimed to identify denitrifying and DNRA bacteria isolated from the recirculating cultivation of S. serrata. The water samples were collected from anaerobic filters called close filter system, which is anaerobically conditioned with the addition of varying physical filter materials in the recirculating mud crab cultures. The results showed that three denitrifying bacterial isolates and seven DNRA bacterial isolates were successfully identified. The phylogenetic analysis based on 16S rRNA gene of the denitrifying bacteria revealed that HIB_7a had the closest similarity to Stenotrophomonas daejeonensis strain MJ03. Meanwhile, DNRA bacterial isolate of HIB_92 showed a 100% similarity to Bacillus sonorensis strain N3, Bacillus vallismortis strain VITS-17, Bacillus tequlensis strain TY5, Geobacillus sp. strain DB24, Bacillus subtilis strain A1, and Bacillus mojavensis strain SSRAI21. This study provides basic information denitrifying and DNRA bacterial isolates identity which might have the potential to be applied as probiotics in aquaculture systems in order to maintain optimal environmental conditions.

• Fisheries and Aquatic Sciences
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• v.15 no.4
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• pp.337-343
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• 2012

The Korean bottom trawl survey was conducted using a trawl designed by the National Fisheries Research and Development Institute (NFRDI). The capture efficiency and size selectivity of this trawl for snow crab Chionoecetes opilio was investigated by experimental tows. An auxiliary sampling net (underbag) was attached beneath the trawl net to capture crabs escaping under the trawl footrope. Experimental tows were made by the same vessel speed (3.4 knots) as in the bottom trawl survey, but toing time was shortened from the standard 30 min to 10 min to reduce possible trawl distortion due to the high catch rate of mud and debris in the underbag. In averaged over 17 tows conducted between 110-383 m depth, trawl efficiency of both males and females combined increased from about 10% at 20 mm (carapace width) to about 70% at 100 mm, with a width of 50% capture equal to 78 mm.

• Proceedings of the Korean Society of Crop Science Conference
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• 2001.09a
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• pp.72-90
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• 2001

Environment agriculture carried out from 1999 in Daeho reclaimed saline area, located in central east cost of Choongnam Korea, resulted in dramatic reduction of amount of nitrogen fertilizer and application times and amount of pesticides. The ecological status of Daeho reclaimed saline area was considered to still very sound from the results of ecological survey on flora and fauna. However, it was desirable to adapt precision agriculture for production of high eating quality of rice and preservation of Daeho ecosystem. Especially, precise application of nitrogen and phosphorus fertilizer was recommendable for prevention of water pollution in environment rice cultivation by duck or mud snail or crab. The bioefficacy of Scirpus maritimus and Echinochloa crus-galli in paddy field of environment rice cultivation by duck or mud snail or crab in Daeho reclaimed saline area was revealed very low. Therefor, it was concluded that the pre-measures of reduction of natural weed population were necessary for successful environment agriculture. The most desirable and ideal environmentally sound agriculture in Daeho reclaimed saline area was performance of crop rotation, introduction of legume crops and green manure crops, and also, simultaneous management of crop production and animal husbandry for smooth flow of energy cycle within the closed Daeho ecosystem.

• Parasites, Hosts and Diseases
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• v.48 no.4
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• pp.335-338
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• 2010

Maritrema spp. (Digenea: Microphallidae) are parasites of birds, but have not been found in the Republic of Korea. In this study, metacercariae of Maritrema sp. were discovered in the Asian shore crab, Hemigrapsus sanguineus, caught in the mud-flats of Jebu-do, Hwasung-gun, Gyeonggi-do, and the adult flukes were confirmed by experimental infection into mice. Based on the symmetric ribbon-like vitellarium, adult flukes of Maritrema sp. were identified, but did not belong to previously described species in terms of the following morphologic characteristics: ceca reaching to the lateral wall at the anterior border of the ovary; ventral sucker larger than oral sucker; a prominent metraterm; and vitellarium forming a complete ring. Hence, we named this microphallid M. jebuensis n. sp. after the island where the second intermediate hosts were collected. From this study, it has been shown that Maritrema sp. is distributed in Korea and transmitted by the Asian shore crab, H. sanguineus.

• Journal of the Korean Society of Costume
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• v.62 no.5
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• pp.59-72
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• 2012

This study aims to collect Minhwa, or folk painting, particularly the Lotus Flower Painting, to analyze its formative characteristics and related story, and to develop cultural fashion products by utilizing Korean traditional culture and modern flavor based on the results. Lotus flowers grow from mud, but are unstained, and they bloom beautiful flowers. Thus, they symbolize Gunja, a true gentleman who is very learned and proper in behavior. In Buddhism, lotus flowers are divine flowers that have the meaning of the creation of life and the eternal cycle of birth, death and rebirth. Lotus flowers also represent the love between man and woman as well as conjugal harmony and love. Lotus flower painting includes the paintings of the lotus flower alone as well as the paintings of the lotus flower with ducks, white heron, kingfisher, fish, butterfly, crab, or tortoise. Colors that are mostly used in lotus flower paintings is the compatible combination of red and blue (green). Based on these findings, fashion products such as bag accessories, sitting cushions, and kitchen utensils are developed using various designs such as realistically drawn lotus flower, schematized lotus flower, the lotus flower alone, or the lotus flower with kingfisher, crab, or dragonfly, that emphasizing the compatible color combination of red and blue.

• Korean Journal of Environmental Biology
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• v.32 no.4
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• pp.311-318
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• 2014

PFOS (perfluorooctane sulfonate) is one of the perflourinated organic compound, which persist as a residual compound in the coastal environments. Intertidal mud crab Macrophthalmus japonicus mainly inhabits in coastal and bay ecosystems in Indo-Pacific region including Korea and reflects to environmental changes. In the present study, M. japonicus were exposed to different concentrations of PFOS and various ecotoxicological end-points such as survival rate, elimination of appendages, changes of the crust and internal organ color changes were investigated. Interestingly, the PFOS exposure showed concentration-dependent decrease of survival rate. High PFOS exposure ($30{\mu}gL^{-1}$) showed a low survival rate of 24% at 168 hours. Further, in comparison with the controls, the rate of elimination of appendages was also considerably increased in a time dependent manner upon PFOS exposure. Notably, with progression of time, an increased exposure to PFOS, test species showed whitening effect in a concentration-dependent manner, whereas the crab crust color was unchanged in the control. In addition, change in internal organs color and their visibility (clarity) observed in PFOS exposed crabs compared to control. Taken together, we suggest, eco-toxicology end-points of M. japonicus exposed to PFOS gave important biological information which could be useful to identify toxic contamination in the marine benthic environments.