• Korean Journal of Agricultural Science
• /
• v.19 no.1
• /
• pp.33-39
• /
• 1992

Effects of buprofezin, a chitin synthesis inhibitor of insects, on mycelial growth, protoplast formation and reversion of Coriolus versicolor, Ganoderma applanatum and G. lucidum were investigated. The mycelial growth of C. versicolor, G, applanatum and G, lucidum was severely inhibited by buprofezin treatment, and the inhibition rate severely as the concentration of the buprofezin increased. Aerial mycelia and oidia formation of the mushrooms were increased by buprofezin treatment, but mycelial morphology was not changed. The rate of protoplast formation and reversion of G, applanatum was greatly increased by the treatment of the buprofezin, while that of G. lucidium was decreased. The rate of protoplast formation of C. versicolor was also increased when buprofezin was added to the medium, but the rate of protoplast reversion was not affected by the treatment, From the results obtained in this experiment, we found that the rate of protoplast. formation and reversion was increased by the treatment of the buprofezin in the mushrooms such as C, versicolor and G, applanatum, whose mycelial growth was fast on the medium, while that of G. lucidum, whose mycelial growth was relatively slow, was decreased by the treatment.

• Korean Journal of Agricultural Science
• /
• v.17 no.2
• /
• pp.77-81
• /
• 1990

Effects of buprofezin, an inhibitor of chitin synthesis, on mycelial growth, protoplast formation and reversion of Pleurotus ostreatus and P. sajor-caju were investigated. The mycelial growth of Pleurotus ostreatus and P. sajor-caju was the inhibited by buprofezin treatment, and the inhibition rate was severer as the concentration of the buprofezin increased. Aerial mycelium formation was increased by buprofezin treatment, but mycelial morphology was not changed. Protoplast formation of Pleurotus ostreatus and P. sajor-caju. was significantly increased when buprofezin was added to the culture medium at the concentration of 200~500 ppm and the protoplast reversion of the mushrooms was also increased by the treatment of the buprofezin.

• Microbiology and Biotechnology Letters
• /
• v.21 no.1
• /
• pp.59-65
• /
• 1993

We isolated Actinomycetes strain GMT 1155 and purified the active compound, MT 1155, on the morphological reversion of ts/NRK cell from the isolate. MT 1155 was identified as toyocamycin having antifungal and antitumor activities from physico-chemical properties and UV, IR, $^1H$-NMR, $^13C$-NMR and mass spectrum. MT 1155 showed the morphologically reversional activity on ts/NRK cell and the cytotoxicity on CTLL cell at the final concentrations of 1.7 JlM and 0.2 11M, respectively and its $IC_{50}$ value on protein kinase A enzyme was 2.3 $\mu$M. Also it had strong antifungal activity against several pathogenic fungi but not antibacterial activity. And it did not inhibit both protein kinase C activity and the bleb-formation of K562 cell induced by phorbol esters.

• The Korean Journal of Zoology
• /
• v.17 no.2
• /
• pp.51-56
• /
• 1974

Newborn Armenian and Chinese hamsters inoculated with adenovirus type 12 developed undifferentiated small cell tumors as early as 27 days after inoculation in the Chinese hamsters and within 30$\\sim$45 days in the Armenian hamsters. These tumors were transplantable and epithelial-like cell in morphology. Cultures of 6 tumors underwent spontaneous reversion to fibroblast-like morphology during the 14$\\sim$20 in vitro passages in the absence of chromosomal disturbances. While epithelial-like tumor derivatives were oncogenic and positive for the T-antigen, fibroblast-like revertants were non-oncogenic and negative for the T-antigen. Two other tumor derivatives reverted to fibroblast-like forms, immediately following exposure to SV40. These lacked the adenovirus T-antigen but were positive for the SV40 T-antigen and formed sarcomas in animals.

• Journal of Korean Society of Forest Science
• /
• v.1 no.1
• /
• pp.7-11
• /
• 1962

1. The inflorescence of the oak has been evolved through a serial reduction of the panicle as is presented in the figure 1. 2. Perianth lobes though they range from one to twelve in a flower six loves occur more than 50% of tested flowers as is tabulated in the table 1. They have been increased partly by a fusion of flowers and partly by a reversion of the stamen into the perianth lobe. They also have been decreased by a fusion of perianth lobes each other. 3. Stamen numbers in a flower range from one to twenty four, but eight stamens are more common than the others as is shown in the table 1. They have been increased by a fusion of flowers and decreased by a fusion of stamens in a flower as are presented in the figure 4. 4. Floral position in the inflorescence, environmental factors, hybridity, and abnormal nutrition supply seem to accelerate fusion and reduction of the stamen in a flower. 5. Flowers which consist of eight stamens and six perianth lobes occur more common than the others, however, the number of the stamen (Y) and the perianth lobes (X) appear to be correlated as follows (see also fig.5.). Y=1.09X+3.78, r=0.4 for Q. dentate Thumb. (7004 flowers investigated) Y=0.71X+4.18, r=0.48 for Q. mongolica Fischer (7409 flowers investigated) Y=1.03X+2.91, r=0.4 for Q. allena B1. (8662 flowers investigated) Y=0.69X+2.00, r=0.4 for Q. serrata Thumb. (9048 flowers investigated) 6. Morphological similarity of the bracteoles and perianth lobes as illustrated in the fig. 3, phenomena of a fusion of bracteoles and separation of perianth lobes into three groups in a flower, and increasing and decreasing phenomena of the stamen number and perianth lobes in a flower as presented in the figures 3 and 4 support the proposal on the oak flower evolution made by H. Hjelmquist in 1948.