• 건축역사연구
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• 제13권2호
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• pp.21-38
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• 2004

This study provides a descriptive and analytical account of major aspects of urban development and transformation of housing types of Italian Firenze from the 13th century to the 19th century. It is a typo-morphological depiction of urban spatial structure of the extraordinary city, Firenze, the center of Italian Renaissance. And this study has proceeded on the assumption that the evolving form of the urban structure and housing types cannot be understood without reference to the larger context of political, economic, and social life. Based on these backgrounds, the purpose of this study is threefold: to provide a comprehensive discussion of general characteristics of urban spatial structure of Firenze, and to explain the process of formation of working-class neighborhoods by constructing new city wall in later 13th century, and to discuss transformation of housing types of the working-class neighborhood with understanding the mechanism of existence of housing in the newly formed residential neighborhoods. The development of residential neighborhoods was pursued by 'planned' manner through forming square-shaped blocks, and characterized by the subdivision of larger properties into standardized building lots for the construction of houses. On the bases of documentary evidences, several ecclesiastical institutions are identified as the agents of a distinctive type of development. While the institutions did the major role for developing lands, the construction of houses was done by small scale construction agents with moderate amount of properties. The major housing type of working-class neighborhoods of Firenze has been the 'casa a schiera' characterized by the form of narrow front and long depth. The type was generalized by the newly formed middle and working-class of Firenze which grew their body very rapidly, Even though the type assumed very uniform in its fen there were many variations. And through passing time, the casa a schiera developed to be multi-family housing, and the level of variation became deepen. Eventually, transformation of housing type of Firenze was ended by appearance of the 'casa in linea', which was very similar to modern apartment in its spatial organization.

• 한국콘텐츠학회논문지
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• 제6권12호
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• pp.147-154
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• 2006

본 연구는 심양성의 도시평면에 대한 형태학적 분석을 토대로 심양 도성형태의 형성 변화과정에서 표출되는 형태적 특성을 도출하는 한편, 이를 정치 사회 문화적 여건변화와 문화적 상호작용 측면에서 해석해보고자 하는데 목적이었다. 이를 위해 심양의 도시구조를 시계열 적으로 고찰하고, 이를 표현할 수 있는 지도정보와 도면정보를 GIS DB로 구축하여 시계열별로 중첩비교하여 역사적인 관점에서 해석해보고자 하는 것이다. 연구를 통해 도출된 심양의 도시 형태적 특성은 첫째 개방적인 도성구조에서 폐쇄적인 도성구조로의 변화과정, 둘째 횡축기반의 수평적 공간구조의 종축기반의 수직적 공간구조의 결합, 셋째 실용적인 도성 구조로부터 축과 중심성에 기초한 상징적인 도성구조로의 변화, 넷째, 자연에 순응한 도성구조로부터 중국고대도성제에 기초한 도성구조로의 변화, 다섯째 만족 고유의 문화적 전통의 보존 등의 다섯 가지 측면으로 파악되었다.

• 한국작물학회지
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• 제54권4호
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• pp.422-426
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• 2009

Sweetpotato is one of the important starch crops, current more considered as an industrial crop rather than food because it has higher starch content (over 80% of biomass), it is used for bio resources for industrial area. In this study, we generated S598A (a mutant gene of oat phytochrome A) sweetpotato plant using Agrobacterium-transformation method. Morphological characteristics of S598A plant were compared with the wild type sweetpotato, S598A had darker green leaves, increased chlorophyll content higher than to two-fold, delayed leaf senescence, shorter plant height (60% shorter than that of the wild type), more number of leaves and petioles about 1.8-fold, shorter petiole length (30% shorter), 1.2-fold more branches and 1.6-fold thicker stem diameters. From this study, S598A plants with such phenotypic characteristics might be able to use the solar energy efficiently, to have increased tolerance to biotic and abiotic stresses and finally to increase productivity (not only starch yield but also root biomass yield). S598A sweetpotato lines are under field trials.

• Applied Microscopy
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• 제46권3호
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• pp.134-139
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• 2016

Detailed structural and molecular imaging of intact organs has incurred academic interest because the associated technique is expected to provide innovative information for biological investigation and pathological diagnosis. The conventional methods for volume imaging include reconstruction of images obtained from serially sectioned tissues. This approach requires intense manual work which involves inevitable uncertainty and much time to assemble the whole image of a target organ. Recently, effective tissue clearing techniques including CLARITY and ACT-PRESTO have been reported that enables visualization of molecularly labeled structures within intact organs in three dimensions. The central principle of the methods is transformation of intact tissue into an optically transpicuous and macromolecule permeable state without loss of intrinsic structural integrity. The rapidly evolving protocols enable morphological analysis and molecular labeling of normal and pathological characteristics in large assembled biological systems with single-cell resolution. The deep tissue volume imaging will provide fundamental information about mutual interaction among adjacent structures such as connectivity of neural circuits; meso-connectome and clinically significant structural alterations according to pathologic mechanisms or treatment procedures.

• 국제초고층학회논문집
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• 제6권2호
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• pp.139-147
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• 2017

Analyzing morphological evolution over a long period of time is deemed an effective way to identify problems occurring in the process of urban development, in addition to achieving a fundamental understanding of socio-cultural changes and growth rooted from the context. As far as the urban morphology is concerned, Hong Kong is characterized by its unique high-density and compact layout patterns, which have aroused the interest of a number of authors in the urban design domain. Whilst an increasing number of redevelopment projects in Hong Kong were criticized for ignoring and destroying the old urban fabric, there is a need for research to investigate the origins and changes of various urban patterns and their implications for society. By employing the theories and techniques of space syntax, this paper accordingly provides a morphological analysis based on the Wanchai District - a 'Segment-line' city, which particularly epitomizes various urban grids of Hong Kong and may have different implications for functional aspects. By axial-mapping the urban layouts of five stages of growth since 1842 and subsequently investigating their spatial and functional transformation over the past 170 years, this paper identifies a series of spatial characteristics underlying different grid patterns, as well as achieves a precise understanding of their ever changing relationship. Based on these understandings, this paper intends to provide valuable reference and guidance for upcoming spatial development in Hong Kong and other regions.

• 한국주조공학회지
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• 제12권5호
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• pp.403-411
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• 1992

The growth characteristics of bainite at early stage in the fast quenched spheroidal graphite cast irons containing 0.06%Mn and 0.45%Mn during austempering process, was investigated with optical and scanning electron microscope. The following results regarding the effects of Mn and isothermal heat treatment on the morphological variation of bainitic ferrite were obtained. The morphology of bainite varies from acicular below 350$^{\circ}C$ to feather shape above 350$^{\circ}C$. The period of isothermal treatment also affects the shape of bainite at the fixed temperature. At 350$^{\circ}C$, bainite is bamboo leaf-like up to 200 secs of isothermal holding time and with further increasing time up to 300 secs, changes to a mixed structure consisting of both feather and bamboo leaf and, finally becomes all feather shape at 900 secs. The morphology of bainitic ferrite formed at early stage of 300$^{\circ}C$ isothermal treatment is similar to that of bainitic ferrite formed at 250$^{\circ}C$ or 350$^{\circ}C$ with unbranched, linear ferrite. However, bainitic ferrite divides into branches with increasing isothermal treatment, which occurs more fast at 400$^{\circ}C$ than at 350$^{\circ}C$. The difference in adding amount of Mn influences the morphology of bainitic ferrite in upper bainite. The bainitic ferrite with 0.45%Mn is observed to be more stable than that with 0.06%Mn, remaining unbranched for a longer period at the same temperature.

• 한국해양공학회지
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• 제37권2호
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• pp.68-79
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• 2023

Even though submerged breakwater reduces incident wave energy, it redistributes the coastal area's wave-induced current, sediment transport, and morphological change. This study examines the coastal hydrodynamics and the morphological response of a wave-dominated beach with submerged breakwaters installed through field observation and quasi-3D numerical modeling. The pre-and post-storm bathymetry, water level, and offshore wave under storm forcing were collected in Bongpo Beach on the East coast of Korea and used to analyze the coastal hydrodynamic response. Four vertically equidistant layers were used in the numerical simulation, and the wave-induced current was examined using quasi-3D numerical modeling. The shore normal incident wave (east-northeast) generated strong cross-shore and longshore currents toward the hinterland of the submerged breakwater. However, the oblique incident wave (east-southeast) induced the southeastward longshore current and the sedimentation in the northeast area of the beach. The results suggested that the incident wave direction is a significant factor in determining the current and sediment transport patterns in the presence of the submerged breakwaters. Moreover, the quasi-3D numerical modeling is more appropriate for estimating the wave transformation, current, and sediment transport pattern in the coastal area with the submerged breakwater.

• 한국농촌건축학회논문집
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• 제13권4호
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• pp.115-124
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• 2011

This study examines the process of urban formation in the city of Yanji. The analysis is done by the change of street pattern and major facility location in a timely manner by appling space syntax method. This research categorizes the pattern of the urban growth focused on two urban forms, street and major buildings, in the city and is offered of the meaning of the first value on interpretation of the urban growth in the city of Yanji. The result of the research are as follows. First, the period of the city transformation is divided into four stage; intuitive period, grid-iron formative period, consolidation period, and urban expansion period. Second, characteristics of each stage have been analyzed. At the first stage, the city started to frame along both buildings and streets which were placed without a plan. At the second stage, the city was planned and constructed into new grid-iron pattern ignoring existing context by Jananese colony. At the consolidation period, the road system expands from the city center toward suburban. At the last expansion period, the shape of the city has complicated its shape with complex road system. This study contributes to provide a basic analysis and data to investigate how the Yanji city has evolved over period of time. But further continuous research should be done for future urban development of the Yanji city.

• 한국초지조사료학회지
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• 제21권1호
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• pp.21-26
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• 2001

환경 스트레스에 의해 야기되는 활성 산소종에 의한 피해에 내성을 가지는 목초의 개발을 위하여 오차드그래스의 배반 조직 유래의 캘러스에 배추유래의 cytosolic glutathione reductase 유전자(BcGRl)를 Agrobucterium tumefaciens EHA101을 매개로 형질전환시켰다. Hygromcin으로 선발된 캘러스로부터 재분화된 식물체는 야생형과 비교하여 형태적으로 차이를 나타내지 않았다. PCR 및 Southern blot 분석을 통하여 형질전환 식물체의 염색체 내에 BcGRl 유전자가 integration 되었음을 확인하였다. 오차드그래스의 잎으로부터 total RNA를 분리하여 Northern blot 분석을 실시한 결과, 도입된 유전자가 형짙전환 식물체 내에서 지속적으로 발현된다는 것을 확인하였다.

• Journal of Plant Biotechnology
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• 제37권2호
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• pp.174-185
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• 2010

Full-length cDNAs are essential for the correct annotation of genomic sequences and for the functional analysis of genes and their products. To elucidate the functions of a large population of Chinese cabbage (Brassica rapa) genes and to search efficiently for agriculturally useful genes, we have been taking advantage of the full-length cDNA Over-eXpresser (FOX) gene hunting system. With oligo dT column it purify the each mRNA from the flower organs, leaf and stem tissue. And about 120,000 cDNAs from the library were transformed into $\lambda$-pFLCIII-F vector. Of which 115,000 cDNAs from the library were transformed into T-DNA binary vector, pBigs for transformation study. We used normalized full-length cDNA and introduced each cDNA into Arabidopsis by in planta transformation. Full-length Chinese cabbage cDNAs were expressed independently under the CaMV 35S promoter in Arabidopsis. Selfed seeds were harvested from transgenic Arabidopsis. We had selected 2,500 transgenic plants by hygromycin antibiotic tolerant test, and obtained a number of transgenic mutants. Each transgenic Arabidopsis was investigated in morphological changes, fertility and leaf colour. As a result, 285 possible morphological mutants were identified. Introduced cDNA was isolated by PCR amplification of the genomic DNA from the transgenic mutants. Sequencing result and BLAST analysis showed that most of the introduced cDNA were complete cDNAs and functional genes. Also, we examined the effect of Bromelain on enhancing resistance to soft rot in transgenic Chinese cabbage 'Osome'. The bromelain gene identified from FOX hunting system was transformed into Chinese cabbage using Agrobacterium methods. Transformants were screened by PCR, then RT-PCR and real time PCR were performed to analyze gene expression of cysteine protease in the T1 and T2 generations. The anti-bacterial activity of bromelain was tested in Chinese cabbages infected with soft rot bacteria. The results showed that the over-expressed bromelain gene from pineapple conferred enhanced resistance to soft rot in Chinese cabbage.