• Korean Journal of Weed Science
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• v.17 no.3
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• pp.256-261
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• 1997

Effect of a new postemergence herbicide carfentrazone-ethyl on controlling weeds in pear orchards was examined and compared with that of glyphosate and glufosinate. Herbicides tested were applied in mid June when weeds had reached at an average height of 15 to 20cm. The experimented orchards were infested mainly by dicots of Artemisia princeps, Chenopodium album, Polygonum hydropiper, Erigeron canadensis, Commelina cummunis, Calystegia japonica, and Amaranthus ascenders, and monocots of Echinochlor crus-galli, Digitaria sangunalis, Setaria viridis, and several sedges, Carfentrazone-ethyl alone effectively controlled dicot weeds in the orchards, but not monocot weeds. Especially, sedges were not adequately controlled by carfentrazone-ethyl alone at any application rates examined. Glyphosate or glufosinate was more effective to control monocot weeds than carfentrazone-ethyl, whereas carfentrazone-ethyl exhibited higher effect on dicot weeds than glyphosate or glufosinate. Carfentrazone-ethyl mixed with glyphosate of the half recommended rate exhibited a higher degree of weed control, especially of E. crus-galli, as compared to the herbicide mixture of carfentrazone-ethyl with glufosinate of the half recommended rate. However, no phytotocixity of the herbicides to pear trees was observed. Our results demonstrate that catfentrazone-ethyl is useful to reduce the application rates and to accelerate the weed controlling effect of glyphosate or glufosinate. Thus, carfentrazone-ethyl in combination with glyphosate or glufosinate can successfully be used to control the most important weeds in orchards.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.48 no.2
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• pp.127-133
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• 2003

The purpose of this study is to investigate the positive or negative effects of 5-aminolevulinic acid(ALA) on the growth of several crops and weeds, by applying a seed soaking treatment, foliar treatment, and application timing, while comparing biological activity between ALA produced by chemical synthesis (Synthetic-ALA) and extracellularly-accumulated ALA by overexpressing the hemeA gene isolated from Bradyrhizobium japonicum(Bio-ALA). Seed soaking treatment of ALA in barley (five cultivars) and wheat (five cultivars) had not shown positive effects at lower concentrations, 0.05 to 0.5 mM as well as negative effects at higher concentrations, 1 to 30 mM. In rice, there also was no positive effect by seed soaking treatment of ALA at lower concentrations, although the rice became damaged by an application of 5 and 10 mM ALA. Growth in barley cultivars, Ganghossalbori, Naehanssalbori, Songhakbori, Saessalbori, and Daehossalbori were increased up to 14%, 19-51 %, 17-64%, 18-23%, and 22-38% by ALA foliar application at lower concentrations, 0.05 to 0.5 mM, respectively. On the other hand, the growth in barley cultivars was inhibited by ALA foliar application at higher concentrations. Barley responded more positively to ALA foliar application than wheat and rice. The growth stimulation caused by ALA seed soaking treatment was less than by ALA foliar treatment. ALA treatment at the 1.5-leaf stage increased growth of barley by 19-58%, while pretreatment to seeds, post-emergence treatment at 3 days after seeding, 3-leaf stages, and 5-leaf stages had not shown positive effects. Thus, the positive effects of ALA on barley were dependent greatly upon the timing of application and its concentration. Monocots weeds were more sensitive to ALA foliar treatment than dicotyledonous weeds. A monocot weed, Setaria viridis L. was the most susceptible plant to ALA while a dicotyledonous weed, Plantago asiatica L. was the most tolerant. No significant difference in biological activity between bio-ALA and synthetic ALA on barley, wheat, rice, and weed, Ixeris dentate tested was observed. Thus, ALA produced by microorganisms would be a potent substance to be used effectively in agricultural production.

• Journal of Life Science
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• v.18 no.1
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• pp.52-57
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• 2008

Xylan is a major hemicellulose component of the cell walls of monocots and hardwood, representing up to 30% of the dry weight of these plants. To efficiently hydrolyze xylan, the endoxylanase gene from Bacillus sp. was expressed in B. subtilis DB431 by introducing the plasmid pJHKJ4. The total activity of the recombinant endoxylanase reached about 857 unit/ml by batch fermentation of B. subtilis DB431/pJHKJ4 in LB maltose medium. The majority (>92%) of endoxylanase was efficiently secreted into the culture medium. The recombinant endoxylanase hydrolyzed more the birchwood xylan efficiently than the other xylans. When 4 % concentration of xylan was used, the highest production of xylooligosaccharide was observed, and xylobiose and xylotriose were the major products. Optimal amount of enzyme and reaction time for producing xylooligosaccharide were found to be 10 unit and 1 hr, respectively. In addition, the temperature of $40^{\circ}C{\sim}50^{\circ}C$ gave the highest production of xylooligosaccharide. Consequently, the optimized conditions for the production of xylooligosaccharide through the hydrolysis of xylan were determined as follows: 10 unit endoxylanase, $50^{\circ}C$, 4% birchwood xylan, 1 hr reaction.