• 미생물학회지
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• 제55권1호
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• pp.55-57
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• 2019

페놀과 같은 단환성 화합물을 분해하는 미생물인 Runella sp. ABRDSP2 균주는 담수로부터 분리되었다. 원형으로 완성된 하나의 chromosome과 3개의 plasmid로 구성된 유전체는 GC 함량이 44.4%인 총 7,613,819 bp의 크기를 나타내며 6,006개의 유전자를 인코딩하고 있다. ABRDSP2 균주는 monooxygenase, ring-cleaving dioxygenase 및 catechol 1,2-dioxygenase 등의 다수의 방향성 탄화수소를 분해하는 유전자를 함유하고 있다. 이런 전장 유전체는 Runella sp. ABRDSP2 균주가 다양한 생분해능력이 있음을 나타낸다.

• Journal of Microbiology and Biotechnology
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• 제14권2호
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• pp.302-311
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• 2004

Comamonas testosteroni (formerly Pseudomonas sp.) NCIMB 10643 can grow on biphenyl and alkylbenzenes $(C_2-C_7)$ via 3-substituted catechols. Thus, to identify the genes encoding the degradation, transposon-mutagenesis was carried out using pAG408, a promoter-probe mini-transposon with a green fluorescent protein (GFP), as a reporter. A mutant, NT-1, which was unable to grow on alkylbenzenes and biphenyl, accumulated catechols and exhibited an enhanced expression of GFP upon exposure to these substrates, indicating that the gfp had been inserted in a gene encoding a broad substrate range catechol 2,3-dioxygenase. The genes (2,826 bp) flanking the gfp cloned from an SphI-digested fragment contained three complete open reading frames that were designated bphCDorfl. The deduced amino acid sequences of bphCDorfl were identical to 2,3-dihydroxybiphenyl 1,2-dioxygenase (BphC), 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate hydrolase (BphD), and OrfI, respectively, that are all involved in the degradation of biphenyl/4-chlorobiphenyl (bph) by Ralstonia oxalatica A5. The deduced amino acid sequence of the orfl revealed a similarity to those of outer membrane proteins belonging to the OmpW family. The introduction of the bphCDorfl genes enabled the NT-l mutant to grow on aromatic hydrocarbons. In addition, PCR analysis indicated that the DNA sequence and gene organization of the bph operon were closely related to those in the bph operon from Tn4371 identified in strain A5. Furthermore, strain A5 was also able to grow on a similar set of alkylbenzenes as strain NCIMB 10643, demonstrating that, among the identified aromatic hydrocarbon degradation pathways, the bph degradation pathway related to Tn4371 was the most versatile in catabolizing a variety of aromatic hydrocarbons of mono- and bicyclic benzenes.