• Title/Summary/Keyword: molecular survey

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The production and application of therapeutic 67Cu radioisotope in nuclear medicine

  • Kim, Gye-Hong;Lee, Kyo Chul;Park, Ji-Ae;An, Gwang-Il;Lim, Sang Mo;Kim, Jung Young;Kim, Byung Il
    • Journal of Radiopharmaceuticals and Molecular Probes
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    • v.1 no.1
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    • pp.23-30
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    • 2015
  • Radioisotopes emitting low-range highly ionizing radiation such as ${\beta}$-particles are of increasing significance in internal radiotherapy. Among the ${\beta}$-particle emitting radioisotopes, $^{67}Cu$ is an attractive radioisotope for various nuclear medicine applications due to its medium energy ${\beta}$-particle, gamma emissions, and 61.83-hour half-life, which can also be used with $^{64}Cu$ for PET imaging. The production and application of the ${\beta}$-emitting radioisotope $^{67}Cu$ for therapeutic radiopharmaceutical are outlined, and different production routes are discussed. A survey of copper chelators used for antibody labeling is provided. It has been produced via proton, alpha, neutron, and gamma irradiations followed by solvent extraction, ion exchange, electrodeposition. Clinical studies using $^{67}Cu$-labelled antibodies in lymphoma, colon carcinoma and bladder cancer patients are reviewed. Widespread use of this isotope for clinical studies and preliminary treatments has been limited by unreliable supplies, cost, and difficulty in obtaining therapeutic quantities.

Accumulation of Transcripts Abundance after Barley Inoculation with Cochliobolus sativus

  • Arabi, Mohammad Imad Eddin;AL-Daoude, Antonious;Shoaib, Amina;Jawhar, Mohammad
    • The Plant Pathology Journal
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    • v.31 no.1
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    • pp.72-77
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    • 2015
  • Spot blotch caused by the hemibiotrophic pathogen Cochliobolus sativus has been the major yield-reducing factor for barley production during the last decade. Monitoring transcriptional reorganization triggered in response to this fungus is an essential first step for the functional analysis of genes involved in the process. To characterize the defense responses initiated by barley resistant and susceptible cultivars, a survey of transcript abundance at early time points of C. sativus inoculation was conducted. A notable number of transcripts exhibiting significant differential accumulations in the resistant and susceptible cultivars were detected compared to the non-inoculated controls. At the p-value of 0.0001, transcripts were divided into three general categories; defense, regulatory and unknown function, and the resistant cultivar had the greatest number of common transcripts at different time points. Quantities of differentially accumulated gene transcripts in both cultivars were identified at 24 h post infection, the approximate time when the pathogen changes trophic lifestyles. The unique and common accumulated transcripts might be of considerable interest for enhancing effective resistance to C. sativus.

QUANTIFYING DARK GAS

  • LI, DI;XU, DUO;HEILES, CARL;PAN, ZHICHEN;TANG, NINGYU
    • Publications of The Korean Astronomical Society
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    • v.30 no.2
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    • pp.75-78
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    • 2015
  • A growing body of evidence has been supporting the existence of so-called "dark molecular gas" (DMG), which is invisible in the most common tracer of molecular gas, i.e., CO rotational emission. DMG is believed to be the main gas component of the intermediate extinction region from Av~0.05-2, roughly corresponding to the self-shielding threshold of $H_2$ and $^{13}CO$. To quantify DMG relative to $H{\small{I}}$ and CO, we are pursuing three observational techniques; $H{\small{I}}$ self-absorption, OH absorption, and THz $C^+$ emission. In this paper, we focus on preliminary results from a CO and OH absorption survey of DMG candidates. Our analysis shows that the OH excitation temperature is close to that of the Galactic continuum background and that OH is a good DMG tracer co-existing with molecular hydrogen in regions without CO. Through systematic "absorption mapping" by the Square Kilometer Array (SKA) and ALMA, we will have unprecedented, comprehensive knowledge of the ISM components including DMG in terms of their temperature and density, which will impact our understanding of galaxy evolution and star formation profoundly.

Report of 21 unrecorded bacterial species in Korea belonging to the phylum Actinobacteria, discovered during the survey in 2020

  • Ham, You Ju;Jeong, Ji Won;Im, Wan-Taek;Kim, Won-Yong;Yoon, Jeong-Hun;Kim, Myung Kyum;Seong, Chi Nam;Kim, Seung Bum
    • Journal of Species Research
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    • v.11 no.1
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    • pp.1-9
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    • 2022
  • The phylum Actinobacteria includes many groups of aerobic, Gram-stain-positive, rod, or filamentous shaped bacteria. Actinobacteria are known for multicellular differentiation in some groups, and also for production of various secondary metabolites such as antibiotics. During a series of extensive surveys of indigenous prokaryotic species diversity in Korea, bacterial strains belonging to Actinobacteria were isolated from various sources of terrestrial environments. A total of 21 bacterial strains, belonging to 10 genera in 8 families, were isolated as unrecorded species in Korea. Among them, 11 were assigned to the family Streptomycetaceae, two species assigned to each of the families Microbacteriaceae, Mycobacteriaceae and Nocardioidaceae, and one species assigned to each of the families Euzebyaceae, Corynebacteriaceae, Micrococcaceae and Intrasporangiaceae. At the genus level, Streptomyces (10 species) was the most abundant, followed by Microbacterium and Mycolicibacterium(2 species each), and one species in each of the genera Corynebacterium, Euzebya, Arthrobacter, Terracoccus, Kribbella, Nocardioides and Yinghuangia. The detailed descriptions of each unrecorded species are provided.

TRAO Multi-beam Legacy Survey of Nearby Filamentary Molecular Clouds : Progress Report

  • Kim, ShinYoung;Chung, Eun Jung;Lee, Chang Won;Myers, Philip C.;Caselli, Paola;Tafalla, Mario;Kim, Gwanjeong;Kim, Miryang;Soam, Archana;Gophinathan, Maheswar;Liu, Tie;Kim, Kyounghee;Kwon, Woojin;Kim, Jongsoo
    • The Bulletin of The Korean Astronomical Society
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    • v.42 no.1
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    • pp.32.1-32.1
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    • 2017
  • To dynamically and chemically understand how filaments, dense cores, and stars form under different environments, we are conducting a systematic mapping survey of nearby molecular clouds using the TRAO 14 m telescope with high ($N_2H^+$ 1-0, $HCO^+$ 1-0, SO 32-21, and $NH_2D$ v=1-0) and low ($^{13}CO$ 1-0, $C^{18}O$ 1-0) density tracers. The goals of this survey are to obtain the velocity distribution of low dense filaments and their dense cores for the study of their origin of the formation, to understand whether the dense cores form from any radial accretion or inward motions toward dense cores from their surrounding filaments, and to study the chemical differentiation of the filaments and the dense cores. Until Feb. 2017, the real OTF observation time is 460 hours. We have almost completed mapping observation with four molecular lines ($^{13}CO$ 1-0, $C^{18}O$ 1-0, $N_2H^+$ 1-0, and $HCO^+$ 1-0) on the five regions of molecular clouds (L1251 of Cepheus, Perseus west, Polaris south, BISTRO region of Serpense, California, and Orion B). The maps of a total area of $7.38deg^2$ for both $^{13}CO$ and $C^{18}O$ lines and $2.19deg^2$ for both $N_2H^+$ and $HCO^+$ lines were obtained. All OTF data were regridded to a cell size of 22 by 22 arcseconds. The $^{13}CO$ and $C^{18}O$ data show the RMS noise level of about 0.22 K and $N_2H^+$ and $HCO^+$ data show about 0.14 K at the velocity resolution of 0.06 km/s. Additional observations will be made on some regions that have not reached the noise level for analysis. We are refining the process for a massive amount of data and the data reduction and analysis are underway. This presentation introduces the overall progress from observations to data processing and the initial analysis results to date.

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Occurrence of Clubroot Caused by Plasmodiophora brassicae in Baecheongchae

  • Kim, Wan-Gyu;Oh, Sang-Keun;Semunyana, Marc;Han, Man-Jong;Lee, Gyo-Bin;Cho, Weon-Dae
    • The Korean Journal of Mycology
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    • v.48 no.4
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    • pp.499-503
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    • 2020
  • Clubroot symptoms were frequently observed on the roots of Baecheongchae plants grown in vinyl greenhouses of a farmer located in Yangpyeong area of Korea during a disease survey in June 2019. The incidence of diseased Baecheongchae plants ranged from 30 to 90% in the vinyl greenhouses investigated. Many resting spores were found in the tissue of root galls collected. The resting spores were hyaline and spherical and measured 2.5-4.2 ㎛ in diameter. Three inoculum suspensions of resting spores prepared from the root galls were inoculated to the roots of healthy Baecheongchae plants. All the inoculum suspensions caused clubroot symptoms to appear on the roots of the inoculated Baecheongchae plants. The symptoms on the roots induced by artificial inoculation were similar to those observed in the plants of the vinyl greenhouses during the disease survey. Resting spores of the pathogen were recovered from the root galls of the inoculated plants. Three root gall isolates obtained from the inoculated plants were used for molecular identification. Comparing the isolates to the Plasmodiophora brassicae strains in GenBank, the amplification products demonstrated 100% similarity with the internal transcribed spacer (ITS2) sequences. The clubroot pathogen was identified as P. brassicae according to its morphological, pathological, and molecular characteristics. This is the first report of P. brassicae causing clubroot in Baecheongchae.

Near-IR Polarimetry Survey of the Large Magellanic Cloud : Photometric Reliability Test

  • Kim, Jae-Yeong;Pak, Soo-Jong
    • The Bulletin of The Korean Astronomical Society
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    • v.37 no.1
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    • pp.78.1-78.1
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    • 2012
  • We present near-IR imaging polarimetry of the 5${\times}$9 fields (-39'${\times}$69') centered at 30 Doradus in the Large Magellanic Cloud (LMC), using the InfraRed Survey Facility (IRSF). We obtained polarimetry data in J, H, and Ks bands using the JHKs-simultaneous imaging polarimeter SIRPOL in 2008 December and 2011 December. We measured Stokes parameters of point-like sources to derive the degree of polarization and the polarization position angle. Since our results are suffered from non-photometric weather, we compare the polarization results from 2008 and those from 2011, and examine the photometric reliabilities between the two runs. Our survey data will be compared with molecular and dust maps to reveal the large-scale magnetic field properties in the star-forming clouds.

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FEED RESOURCE AVAILABILITY AND UTILIZATION IN SMALLHOLDER PIG FARMS IN SRI LANKA

  • Ravindran, V.;Cyril, H.W.;Nadesalingam, P.;Gunawardene, D.D.
    • Asian-Australasian Journal of Animal Sciences
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    • v.8 no.4
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    • pp.311-316
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    • 1995
  • Data on available feed resources, feeding practices and nutrient adequacy of rations under small farm conditions in Sri Lanka were obtained in a baseline survey involving 104 pig farms. The results showed that a wide range of non-conventional feedstuffs are used for pig feeding under typical small farm conditions and that dietary protein quality is a major factor limiting productivity. Following the survey, two on-farm trials were conducted to evaluate cheaper, alternative feeding strategies. In trial 1, a test diet was formulated using several non-conventional feedstuffs and compared with a commercial feed that is normally fed in the farms. In trial 2, the possibility of improving growth rates by amino acid supplementation was evaluated. The results demonstrated that feed costs can be considerably lowered through these packages. Some problems inherent to on-farm livestock trials are highlighted.

UKIRT Widefield Infrared Survey for [Fe II] $1.64{\mu}m$

  • Lee, Jae-Joon;Koo, Bon-Chul
    • The Bulletin of The Korean Astronomical Society
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    • v.37 no.2
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    • pp.97.1-97.1
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    • 2012
  • NIR Emission lines from singly-ionized Iron, in particular [Fe II] $1.64{\mu}m$, are good tracer of dense atomic gas in star-forming regions, around evolved stars, and in supernova remnants. We are imaging about 180 square degrees along the Galactic Plane ($6^{\circ}$ < l < $65^{\circ}$;$-1.5^{\circ}$ < b < $+1.5^{\circ}$) with the narrow band filter centered on the [Fe II] $1.64{\mu}m$ line using WFCAM at UKIRT. The observations will complement the UWISH2 survey, which have imaged the same area with the narrow band filter centered on the molecular hydrogen 1-0 S(1) emission line at $2.12{\mu}m$, and probe a dynamically active component of ISM. We present the goals and preliminary results of our survey.

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Lack of Association between Herpes Simplex Virus Type 2 Infection and Cervical Cancer - Taq Man Realtime PCR Assay Findings

  • Farivar, Taghi Naserpour;Johari, Pouran;Shafei, Shilan;Najafipour, Reza;Reza, Najafipour
    • Asian Pacific Journal of Cancer Prevention
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    • v.13 no.1
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    • pp.339-342
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    • 2012
  • Background: About one third of the human population suffer cancer during their lifetime and more than 20% of total morbidity is related to neoplasia. Cervical cancer is generally the most common cancer in developing countries and the second most common in women globally. The role of human papilloma viruses viruses in its induction is clear. However, the involvement of hepres simplex virus type 2 (HSV-2) is controversial. Therefore a survey was conducted of the prevalence of HSV-2 in patients with cervical cancer and also healthy people with sensitive and quantitative Taq Man real-time PCR assay. Materials and methods: Seventy six formaldehyde fixed paraffin embedded tissue specimens from patients with histologically proven history of cervical cancer as well as 150 control blocks were sectioned for deparaffinization and DNA extraction. Results: There was no HSV-2 DNA in our patient specimens but four control samples were positive, all with a history of hysterectomy. Conclusion: Considering the absence of any positive viral HSV-2 DNA in our patients and also the presence of four positive specimens among our controls, we did not find any relationship between the presence of HSV-2 DNA and cervical cancer.