• Publications of The Korean Astronomical Society
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• v.27 no.4
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• pp.261-262
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• 2012

The aim of this research is to reveal the spatial distribution of the star formation activity of nearby galaxies by comparing CO molecular emission lines with the large area observation in far-infrared (FIR) lines. We report the imaging observations of NGC 253 by FIR forbidden lines via FIS-FTS and CO molecular lines from low to high excitation levels with ASTE, which are good tracers of star forming regions or photo-dissociation regions, especially spiral galaxies, in order to derive the information of the physical conditions of the ambient interstellar radiation fields. The combination of spatially resolved FIR and sub-mm data leads to the star formation efficiency within galaxy. The ratio between the FIR luminosity and molecular gas mass, $L_{FIR}/M_{H_2}$, is expected to be proportional to the number of stars formed in the galaxy per unit molecular gas mass and time. Moreover the FIR line ux shows current star formation activity directly. Furthermore these can be systematic and statistical data for star formation history and evolution of spiral galaxies.

• Molecules and Cells
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• v.41 no.5
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• pp.413-422
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• 2018

Soybean transgenic plants with ectopically expressed AtABF3 were produced by Agrobacterium-mediated transformation and investigated the effects of AtABF3 expression on drought and salt tolerance. Stable Agrobacterium-mediated soybean transformation was carried based on the half-seed method (Paz et al. 2006). The integration of the transgene was confirmed from the genomic DNA of transformed soybean plants using PCR and the copy number of transgene was determined by Southern blotting using leaf samples from $T_2$ seedlings. In addition to genomic integration, the expression of the transgenes was analyzed by RT-PCR and most of the transgenic lines expressed the transgenes introduced. The chosen two transgenic lines (line #2 and #9) for further experiment showed the substantial drought stress tolerance by surviving even at the end of the 20-day of drought treatment. And the positive relationship between the levels of AtABF3 gene expression and drought-tolerance was confirmed by qRT-PCR and drought tolerance test. The stronger drought tolerance of transgenic lines seemed to be resulted from physiological changes. Transgenic lines #2 and #9 showed ion leakage at a significantly lower level (P < 0.01) than ${\underline{n}}on-{\underline{t}}ransgenic$ (NT) control. In addition, the chlorophyll contents of the leaves of transgenic lines were significantly higher (P < 0.01). The results indicated that their enhanced drought tolerance was due to the prevention of cell membrane damage and maintenance of chlorophyll content. Water loss by transpiration also slowly proceeded in transgenic plants. In microscopic observation, higher stomata closure was confirmed in transgenic lines. Especially, line #9 had 56% of completely closed stomata whereas only 16% were completely open. In subsequent salt tolerance test, the apparently enhanced salt tolerance of transgenic lines was measured in ion leakage rate and chlorophyll contents. Finally, the agronomic characteristics of ectopically expressed AtABF3 transgenic plants ($T_2$) compared to NT plants under regular watering (every 4 days) or low rate of watering condition (every 10 days) was investigated. When watered regularly, the plant height of drought-tolerant line (#9) was shorter than NT plants. However, under the drought condition, total seed weight of line #9 was significantly higher than in NT plants (P < 0.01). Moreover, the pods of NT plants showed severe withering, and most of the pods failed to set normal seeds. All the evidences in the study clearly suggested that overexpression of the AtABF3 gene conferred drought and salt tolerance in major crop soybean, especially under the growth condition of low watering.

• Molecules and Cells
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• v.43 no.2
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• pp.176-181
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• 2020

The RUNX transcription factors serve as master regulators of development and are frequently dysregulated in human cancers. Among the three family members, RUNX3 is the least studied, and has long been considered to be a tumor-suppressor gene in human cancers. This idea is mainly based on the observation that RUNX3 is inactivated by genetic/epigenetic alterations or protein mislocalization during the initiation of tumorigenesis. Recently, this paradigm has been challenged, as several lines of evidence have shown that RUNX3 is upregulated over the course of tumor development. Resolving this paradox and understanding how a single gene can exhibit both oncogenic and tumor-suppressive properties is essential for successful drug targeting of RUNX. We propose a simple explanation for the duality of RUNX3: p53 status. In this model, p53 deficiency causes RUNX3 to become an oncogene, resulting in aberrant upregulation of MYC.

• The Bulletin of The Korean Astronomical Society
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• v.42 no.1
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• pp.32.1-32.1
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• 2017

To dynamically and chemically understand how filaments, dense cores, and stars form under different environments, we are conducting a systematic mapping survey of nearby molecular clouds using the TRAO 14 m telescope with high ($N_2H^+$ 1-0, $HCO^+$ 1-0, SO 32-21, and $NH_2D$ v=1-0) and low ($^{13}CO$ 1-0, $C^{18}O$ 1-0) density tracers. The goals of this survey are to obtain the velocity distribution of low dense filaments and their dense cores for the study of their origin of the formation, to understand whether the dense cores form from any radial accretion or inward motions toward dense cores from their surrounding filaments, and to study the chemical differentiation of the filaments and the dense cores. Until Feb. 2017, the real OTF observation time is 460 hours. We have almost completed mapping observation with four molecular lines ($^{13}CO$ 1-0, $C^{18}O$ 1-0, $N_2H^+$ 1-0, and $HCO^+$ 1-0) on the five regions of molecular clouds (L1251 of Cepheus, Perseus west, Polaris south, BISTRO region of Serpense, California, and Orion B). The maps of a total area of $7.38deg^2$ for both $^{13}CO$ and $C^{18}O$ lines and $2.19deg^2$ for both $N_2H^+$ and $HCO^+$ lines were obtained. All OTF data were regridded to a cell size of 22 by 22 arcseconds. The $^{13}CO$ and $C^{18}O$ data show the RMS noise level of about 0.22 K and $N_2H^+$ and $HCO^+$ data show about 0.14 K at the velocity resolution of 0.06 km/s. Additional observations will be made on some regions that have not reached the noise level for analysis. We are refining the process for a massive amount of data and the data reduction and analysis are underway. This presentation introduces the overall progress from observations to data processing and the initial analysis results to date.

• Journal of The Korean Astronomical Society
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• v.38 no.2
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• pp.291-293
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• 2005

Using 13.7 m telescope of Qinghai station of NAO, PMO at Delin Ha, 43 IRAS sources were mapped with $^{13}CO\;J=1-0\;C^{18}O\;J=1-0$ and CO J=1-0. Each source has one or more cores. The distances of these cores range from 1 pc to several pc, and the masses from $10^2\;M_{\bigodot}$ to $10^5\;M_{\bigodot}$. High velocity outflows were detected. The mass, momentum and energy of these massive cores are larger than those of the low mass ones. With radio, IRAS, MSX data, stellar source distribution were investigated, and sourceless cores that deviate from infrared sources were identified. They are potential high mass star formation sites.

• The Bulletin of The Korean Astronomical Society
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• v.38 no.2
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• pp.67.1-67.1
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• 2013

It has been known that HCN is one of ubiquitous high-density gas tracer, and the abundance ratio between HCN and its Isomer, HNC sensitively depends on kinetic temperature in star-forming regions. Here we investigate the molecular abundance ratio toward three different evolutionary phases of massive star formation: Infrared Dark Clouds, High-mass Protostellar Objects and Ultracompact HII Regions. We obtained the abundances of HCN and HNC using optically thin H13CN and HN13C lines observed with the KVN single-dish telescopes and MAMBO 1.2mm and SCUBA $850{\mu}m$ continuum data. According to our results, the ratio of [HCN]/[HNC] increases statistically with the evolutionary stage, indicative of the effect of temperature. We also found a strong anti-correlation between the column density of molecular hydrogen and the HNC abundance.

• The Bulletin of The Korean Astronomical Society
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• v.42 no.2
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• pp.79.2-79.2
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• 2017

To dynamically and chemically understand how filaments, dense cores, and stars form under different environments, we are conducting a systematic mapping survey of nearby molecular clouds using the TRAO 14 m telescope with high ($N_2H^+$ 1-0, $HCO^+$ 1-0, SO 32-21, and $NH_2D$ v=1-0) and low ($^{13}CO$ 1-0, $C^{18}O$ 1-0) density tracers. The goals of this survey are to obtain the velocity distribution of low dense filaments and their dense cores for the study of their origin of the formation, to understand whether the dense cores form from any radial accretion or inward motions toward dense cores from their surrounding filaments, and to study the chemical differentiation of the filaments and the dense cores. Until the 2017A season, the real OTF observation time is ~760 hours. We have almost completed mapping observation with four molecular lines ($^{13}CO$ 1-0, $C^{18}O$ 1-0, $N_2H^+$ 1-0, and $HCO^+$ 1-0) on the six regions of molecular clouds (L1251 of Cepheus, Perseus West, Polaris South, BISTRO region of Serpens, California, and Orion B). The cube data for $^3CO$ and $C^{18}O$ lines were obtained for a total of 6 targets, 57 tiles, 676 maps, and $7.1deg^2$. And $N_2H^+$ and $HCO^+$ data were added for $2.2deg^2$ of dense regions. All OTF data were regridded to a cell size of 44 by 44 arcseconds. The $^{13}CO$ and $C^{18}O$ data show the RMS noise level of about (0.1-0.2) K and $N_2H^+$ and $HCO^+$ data show about (0.07-0.2) K at the velocity resolution of 0.06 km/s. Additional observations will be made on some regions that have not reached the noise level for analysis. To identify filaments, we are using and testing programs (DisPerSE, Dendrogram, FIVE) and visual inspection for 3D image of cube data. A basic analysis of the physical and chemical properties of each filament is underway.

• The Korean Journal of Physiology and Pharmacology
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• v.16 no.2
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• pp.145-151
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• 2012

In the present work, we studied the structure-activity relationship (SAR) of tautomycetin (TMC) and its derivatives. Further, we demonstrated the correlation between the immunosuppressive fuction, anticancer activity and protein phosphatase type 1 (PP1) inhibition of TMC and its derivatives. We have prepared some TMC derivatives via combinatorial biosynthesis, isolation from fermentation broth or chemical degradation of TMC. We found that the immunosuppressive activity was correlated with anticancer activity for TMC and its analog compounds, indicating that TMC may home at the same targets for its immunosuppressive and anticancer activities. Interestingly, TMC-F1, TMC-D1 and TMC-D2 all retained significant, albeit reduced PP1 inhibitory activity compared to TMC. However, only TMC-D2 showed immunosuppressive and anticancer activities in studies carried out in cell lines. Moreover, TMC-Chain did not show any significant inhibitory activity towards PP1 but showed strong growth inhibitory effect. This observation implicates that the maleic anhydride moiety of TMC is critical for its phosphatase inhibitory activity whereas the C1-C18 moiety of TMC is essential for the inhibition of tumor cell proliferation. Furthermore, we measured $in$ $vivo$ phosphatase activities of PP1 in MCF-7 cell extracts treated with TMC and its related compounds, and the results indicate that the cytotoxicity of TMC doesn't correlate with its $in$ $vivo$ PP1 inhibition activity. Taken together, our study suggests that the immunosuppressive and anticancer activities of TMC are not due to the inhibition of PP1. Our results provide a novel insight for the elucidation of the underlying molecular mechanisms of TMC's important biological functions.

• BMB Reports
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• v.40 no.6
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• pp.1009-1015
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• 2007

In this communication, we report the efficacy of $\beta$-carotene towards differentiation and apoptosis of leukemia cells. Dose ($20{\mu}M$) and time dependence (12 h) tests of $\beta$-carotene showed a higher magnitude of decrease (significance p < 0.05) in cell numbers and cell viability in HL-60 cells than U937 cells but not normal cell like Peripheral blood mononuclear cell (PBMC). Microscopical observation of $\beta$-carotene treated cells showed a distinct pattern of morphological abnormalities with inclusion of apoptotic bodies in both leukemia cell lines. When cells were treated with $20{\mu}M$ of $\beta$-carotene, total genomic DNA showed a fragmentation pattern and this pattern was clear in HL-60 than U937 cells. Both the cell lines, on treatment with $\beta$-carotene, showed a clear shift in $G_1$ phase of the cell cycle. In addition the study also revealed anti-oxidant properties of $\beta$-carotene since there was reduction in relative fluorescent when treated than the control at lower concentration. Collectively this study shows the dual phenomenon of apoptosis and differentiation of leukemia cells on treatment with $\beta$-carotene.

• The Bulletin of The Korean Astronomical Society
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• v.38 no.1
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• pp.50.2-50.2
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• 2013

In the process of star formation, the density and temperature of associated material, which are the physical conditions for the molecular chemistry, vary dramatically. As a result, the connection between physical and chemical conditions has been used to trace the evolutionary stages in star formation. One chemical tracer for the physical conditions in star forming material is the [HCN]/[HNC] abundance ratio since the ratio strongly depends on the kinetic temperature in molecular clouds. Here we investigate the [HCN]/[HNC] abundance ratios in objects related to the massive star formation. For the investigation, we carried out $H^{13}CN$ and $HN^{13}C$ line observation toward objects in three different evolutionary stages of massive star formation: Infrared dark clouds (IRDCs), High-mass protostellar object (HMPOs), and Ultra-compact HII regions (UCHIIs). According to our observational results, both $H^{13}CN$ and $HN^{13}C$ lines have been detected toward 19 IRDCs, 25 HMPOs, and 31 UCHIIs. We will discuss about the [HCN]/[HNC] abundance ratios in different evolutionary stages of massive star formation and associate the results with the physical conditions of the targets.