• Title/Summary/Keyword: molecular distribution

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Isolation and Differential Expression of an Acidic PR-1 cDNA Gene from Soybean Hypocotyls Infected with Phtophthora sojae f. sp. glycines

  • Kim, Choong-Seo;Yi, Seung-Youn;Lee, Yeon-Kyung;Hwang, Byung-Kook
    • The Plant Pathology Journal
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    • v.16 no.1
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    • pp.9-18
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    • 2000
  • Using differential display techniques, a new acidic pathogenesis-related (PR) protein-1 cDNA (GMPRla) gene was isolated from a cDNA library of soybean (Glycinemax L.Merr, cultivar Jangyup) hypocotyls infected by Phytophthora sojae f. sp. glycines. The 741 bp of fulllength GMPRla clone contains an open reading frame of 525 nucleotides encoding 174 amino acid residues (pI 4.23) with a putative signal peptide of 27 amino acids in the N-terminus. Predicted molecular weight of the protein is 18,767 Da. The deduced amino acid sequence of GMPRla has a high level of identity with PR-1 proteins from Brassica napus, Nicotiana tabacum, and Sambucus nigra. The GMPRla mRNA was more strongly expressed in the incompatible than the compatible interaction. The transcript accumulation was induced in the soybbean hypocotyls by treatment with ethephon or DL-$\beta$-amino-n-butyric acid, but not by wounding. In situ hybridization data showed that GMPRIa mRNAs were usually localized in the vascular bundle of hypocotyl tissues, especially phloem tissue. Differences between compatible and incompatible interactions in the timing of GMPRla mRNA accumulation were remarkable, but the spatial distribution of GMPRla mRNA was similar in both interactions. However, more GMPRla mRNA was accumulated in soybean hypocotyls at 6 and 24 h after inoculation.

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Spatial Autocorrelation Analysis among Subpopulations of Salix koriyanagi in Swampy Area at the Namgang River, Korea (남강 습지에 분포하는 키버들 집단의 공간적 상관 분석)

  • Huh, Man-Kyu
    • Journal of Life Science
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    • v.18 no.11
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    • pp.1465-1470
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    • 2008
  • Salix koriyanagi is a deciduous shrub and native to Korea. The spatial distribution of multilocus allelic frequencies and geographical distances of the natural population in upper swampy area at the Namgang River in Korea were studied. The species showed a significant positive and negative spatial autocorrelation according to geographical distances as measured by Moran's I. Genetic similarity of individuals was found among subpopulations at up to a scale of a 12 m distance, and this was partly due to a combination of allelic frequencies, and therefore, a significant spatial autocorrelation was composed of a scale of 12 m intervals. Within S. koriyanagi in swampy area at the Namgang River, a strong spatial structure was observed for allozyme markers, indicating a migration within subpopulations.

A nonlocal strain gradient theory for scale-dependent wave dispersion analysis of rotating nanobeams considering physical field effects

  • Ebrahimi, Farzad;Haghi, Parisa
    • Coupled systems mechanics
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    • v.7 no.4
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    • pp.373-393
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    • 2018
  • This paper is concerned with the wave propagation behavior of rotating functionally graded temperature-dependent nanoscale beams subjected to thermal loading based on nonlocal strain gradient stress field. Uniform, linear and nonlinear temperature distributions across the thickness are investigated. Thermo-elastic properties of FG beam change gradually according to the Mori-Tanaka distribution model in the spatial coordinate. The nanobeam is modeled via a higher-order shear deformable refined beam theory which has a trigonometric shear stress function. The governing equations are derived by Hamilton's principle as a function of axial force due to centrifugal stiffening and displacement. By applying an analytical solution and solving an eigenvalue problem, the dispersion relations of rotating FG nanobeam are obtained. Numerical results illustrate that various parameters including temperature change, angular velocity, nonlocality parameter, wave number and gradient index have significant effect on the wave dispersion characteristics of the understudy nanobeam. The outcome of this study can provide beneficial information for the next generation researches and exact design of nano-machines including nanoscale molecular bearings and nanogears, etc.

Consanguinity Protecting Effect Against Breast Cancer among Tunisian Women: Analysis of BRCA1 Haplotypes

  • Medimegh, Imen;Troudi, Wafa;Omrane, Ines;Ayari, Hajer;Uhrhummer, Nancy;Majoul, Hamdi;Benayed, Farhat;Mezlini, Amel;Bignon, Yves-Jean;Sibille, Catherine;Elgaaied, Amel Benammar
    • Asian Pacific Journal of Cancer Prevention
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    • v.16 no.9
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    • pp.4051-4055
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    • 2015
  • The purpose of this study is to assess the effect of consanguinity on breast cancer incidence in Tunisia. We conducted a case-control study to evaluate the involvement of heterozygote and homozygote haplotypes of BRCA1 gene SNPs according to consanguinity among 40 cases of familial breast cancer, 46 cases with sporadic breast cancer and 34 healthy controls. We showed significant difference in consanguinity rate between breast cancer patients versus healthy controls P=0.001. Distribution of homozygous BRCA1 haplotypes among healthy women versus breast cancer patients was significantly different; p=0.02. Parental consanguinity seems to protect against breast cancer in the Tunisian population.

Mechanical Degradation of Polymers in Dilute Solution (III). The Influence of the Mixed Solvents (稀薄溶液에서의 Polymer의 機械的 切斷 (제3보). 混合溶媒의 영향)

  • Yeong Moo Won
    • Journal of the Korean Chemical Society
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    • v.17 no.3
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    • pp.207-212
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    • 1973
  • The mechanical degradation of poly (-vinylacetate) in several mixed solvents (dioxane-n-butanol, dioxane-sec-butanol, dioxane-ethyleneglycol, dioxane-kerosene.) was studied using the capillary flow method. The velocity constant of scission reaction (k) and the limited degree of polymerization (g) were compared at the same value of [${\eta}$], which is considered as the parameter of molecular dimension of polymers in solution. As result, (k) did not change much, even if the species and the volume fraction of poor (non-) solvents changed, while the value of (g) changed according to the species of poor solvents and the value of [${\eta}$]. From the facts described above, It follows that the limited degree of polymerization (g) were affected by the composition and distribution of mixed solvent molecules around the polymer chain, and the value of $\alpha$ (at [${\eta}$] = $KM^a$) in the polymer solution.

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Behavior of Natural Organic Matter(NOM), Chlorine Residual, and Disinfection By-Products(DBPs) Formation in Pulsed UV Treated Water (Pulsed UV 처리수에서의 자연유기물질, 잔류염소 및 소독부산물 생성 거동)

  • Sohn, Jinsik;Han, Jihee
    • Journal of Korean Society of Water and Wastewater
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    • v.26 no.5
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    • pp.685-692
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    • 2012
  • UV technology is widely used in water and wastewater treatment. Many researches have been conducted on microbial disinfection and micro pollutant reduction with UV treatment. However, the study on NOM with UV has limited because low/medium pressure UV lamp is not sufficient to affect refractory organics such as NOM. Pulsed UV treatment using UV flash lamp can be operated in the pulsed mode with much greater peak intensity. The pulse duration is typically in microseconds, whereas the interval between pulses is in the order of milliseconds. The high intensity of pulsed UV would mineralize NOM itself as well as change the characteristics of NOM. Chlorine demand and DBPs formation is affected on the changed amounts and properties of NOM. The objective of this study is to investigate the effect on NOM, chlorine residual, and chlorinated DBPs formation with pulsed UV treatment.

A Study on the Semiconductor Wastewater Treatment and Recycling by VSEP system (진동막분리장치에 의한 반도체폐수처리와 재이용에 관한 연구)

  • Kang Gyung-Hwan
    • Journal of Environmental Science International
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    • v.14 no.3
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    • pp.335-343
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    • 2005
  • The objective of this research is to evaluate a feasibility of wastewater reuse by membrane treatment with vibrating membrane separation equipment. Molecular weight of compounds in wastewater, permeability of membrane and retentate characterization after membrane filtration were investigated in order to determine appropriate membrane pore size and materials for wastewater treatment. Selected membrane was evaluated with vibration membrane separation equipment to optimize operating conditions. The following conclusion are drawn. 1. We got as following test results after the distribution of particles in the semiconductor wastewater, are made up of $1\~20{\mu}m$. Si, gold and Al in turn are contained in semiconductor wastewater. 2. Recovery rate is changeless under increasing recovery rate in operation. Though a value can be if pressure can be changed, the highest value of permeate rate is presented in 150 psi. 3. The AS-100(polysulpone) was selected as the most appropriate membranes for the treatment of semi-conductor wastewater to VSEP system. The fouling almost did not occur during this experiments. The analyses of treated water with VSEP system showed conductivity: 0.059,us/cm, TDS: 40mg/l, COD: 20mg/l, SS : 5mg/l, n-Hexane: 8.3mg/l. Comparing previous systems, operating expenses is decreased by more $50\%$.

Distribution of Human Papilloma Virus Infections of Uterine Cervix among Women of Reproductive Age - a Cross Sectional Hospital-Based Study from North East India

  • Sarma, Usha;Mahanta, Jagadish;Borkakoty, Biswajyoti;Sarmah, Bidula
    • Asian Pacific Journal of Cancer Prevention
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    • v.16 no.4
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    • pp.1519-1523
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    • 2015
  • Infection of the uterine cervix by human papilloma viruses (HPV) may be associated with cervical pre-cancer and invasive cervical carcinoma if left untreated. With advance in molecular techniques, it has become easier to detect the resence of HPV DNA long before the appearance of any lesion. This study concerned cervical scrape samples of 310 married non-pregnant women attending a gynecology outpatient department for both Pap and PCR testing to detect HPV DNA. Nested PCR using primers for L1 consensus gene with My9/My11 and GP6+/GP5+followed by multiplex PCR were carried out to detect HPV 16 and HPV18. Result: HPV prevalence was 11.9% out of which 3.67% cases of negative for intra-epithelial lesion or malignancy (NILM) and in 71.1% (27/38) of atypical cervical smears were HPV positive. There was increasing trend of high-risk-HPV positivity (HR HPV 16 and 18), from 20% in benign cytology (NILM) to 42.9 % in LSIL, 71.41% in HSIL and 100% in SCC. There was highly significant association of HPV infection with cervical lesion ($x^2=144.0$, p<0.01) and also with type specific HPV prevalence ($x^2=7.761^*$, p<0.05).

Verification on Diffusion of Beam Propagation in Randomly Distributed Scattering Medium (무질서하게 분포된 산란매질에서 빔전파의 확산에 관한 검증)

  • Kim, Ki-Jun;Lee, Hoo-Seol
    • Journal of the Korean Applied Science and Technology
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    • v.24 no.4
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    • pp.354-361
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    • 2007
  • The distribution of light in a randomly scattering medium can represent problems found in many area. Particularly, in the clinical application of lasers for Photodynamic therapy(PDT) or in the fluorescence spectroscopy for biological tissue, turbidity plays a very important role. The influences of fluorophor, scatterer, and absorber in turbid material by light scattering were interpreted for the scattered fluorescence intensity and wavelength. The molecular properties have been studied by laser induced fluorescence spectroscopy in scattering medium as tissue. It has been found that the effects of optical properties in scattering media could be investigated by the optical $parameters({\mu}_s$, ${\mu}_a$ ,${\mu}t)$. Experimental and Monte Carlo simulation method for modelling light transport in tissue was applied. The experimental results using a randomly distributed scattering medium were discussed and compared with those obtained through Monte Carlo simulation. It'll be also important in designing the best model for oil chemistry, medicine and application of medical engineering.