• 제목/요약/키워드: molecular cloning

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싸리에서 isoflavone synthase 유전자의 분리 및 생화학적 특성 (Cloning and Characterization of Isoflavone synthase from Lespedeza bicolor)

  • 김봉규
    • Journal of Applied Biological Chemistry
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    • 제53권1호
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    • pp.60-64
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    • 2010
  • None of genes from Lespedeza bicolor involved in isoflavonoid biosynthesis have been biochemically characterized. An isoflavone synthase from Lespedeza bicolor was cloned. To verify its catalytic activity, a fusion protein of LbIFS with P450 reductase from rice was made. Using this construct, production of isoflavone from flavanone was confirmed.