• 환경생물
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• 제23권2호
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• pp.200-205
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• 2005

This study carried out to certify of taxonomic delimitation in A. artemisiaefolia, A. trifida, A. trifida for. integrifolia, and A. psilostachya in the area of literature and experiment. A. psilostachya was not a vestige of naturalized in Korea and A. trifida and A. trifida for. integrifolia didn't have any valuable differences of morphological and molecular biological experiment. I arranged that naturalized species in Ambrosia are A. artemisiaefolia L. and A. trifida L. in Korea.

• Journal of Astronomy and Space Sciences
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• 제35권2호
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• pp.111-117
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• 2018

A previous exo-terrestrial life-detecting experiment, which was conducted on Mars, sought to detect the products of glucose metabolism, the most common biological process on Earth (Viking biological experiment). Today, glucose metabolism is not considered the universal process of life survival. As NASA plans to launch an orbiter mission in the near future (2020s, the Clipper) and ultimately conduct a lander mission on Europa, a detection experiment that can give broader information regarding habitability is highly required. In this study, we designed a life-detecting experiment using a more universal feature of life, the amphipathic molecular membrane, theoretically considering the environment of Europa (waterdominant environment). This designed experiment focuses on finding and profiling hydrophobic cellular membrane-like microstructures. Expected results are given by conceptual data analysis with plausible hypothetical samples.

• Applied Biological Chemistry
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• 제41권8호
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• pp.568-571
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• 1998

The conformations of human insulin precursors, proinsulin and preproinsulin, are described in terms of molecular dynamics simulations. Despite the presence of the C-peptide and/or the signal peptide, molecular dynamics calculations utilizing the hydration shell model over a period of 500 ps indicate that the native conformations of the A and B chains are well conserved in both cases. These results further support the NMR spectroscopy results that the C-peptide is relatively disordered and does not influence the overall conformation of the native structure. The robustness of the native structure as demonstrated by experiment and simulation will permit future protein engineering applications, whereby the expression or purification yields can be improved upon sequence modification of the C-peptide and/or the signal peptide.

• 한국산업융합학회 논문집
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• 제15권3호
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• pp.71-77
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• 2012

This study gives the optimal reaction conditions, reaction mechanisms, reaction rates leaded from the oxidation of phenol by electron beam accelerator and ozone used for recent water treatment. It gives the new possibility of water treatment process to effectively manage industrial sewage containing toxic organic compounds and biological refractory materials. The high decomposition of phenol was observed at the low dose rate, but at this low dose rate, the reaction time was lengthened. So we must find out the optimal dose rate to promote high oxidation of reactants. The reason why the TOC value of aqueous solution wasn't decreased at the low dose was that there were a lot of low molecular organic acids as an intermediates such as formic acid or glyoxalic acid. In order to use both electron beam accelerator and biological treatment for high concentration refractory organic compounds, biological treatment is needed when low molecular organic compounds exist abundantly in sewage. In this experiment, the condition of making a lot of organic acids is from 5 kGy into 20 kGy dose. Decomposition rate of phenol by electron beam accelerator was first order reaction up to 300ppm phenol solution on the basic of TOC value and also showed first order reaction by using both air and ozone as an oxidants.

• BMB Reports
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• 제33권5호
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• pp.385-390
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• 2000

Overwintering plants should survive the various biotic and abiotic stresses that occur during winter. Previous studies indicated that active oxygen species are involved in freezing, dehydration, anoxia and pathogen infections. As the importance of the events that occur in the intercellular compartment became apparent in disease resistance, we examined the nature of intercellular antioxidant enzymes in order to access their possible involvement in the winter hardiness of barley. The levels of intercellular peroxidase, catalase, and SOD activities on the unit protein basis were 394, 18, and 9% of those of cellular activities, respectively. Major intercellular peroxidase isoforms consisted of four neutrals and four basic forms; whereas major cellular isoforms were two basic forms. Out of the two major catalase isoforms a higher molecular weight form was predominantly abundant in both cellular and intercellular compartments. Among the five major cellular SOD isoforms, three were also present in the intercellular compartment. The presence of substantial amounts of intercellular antioxidant enzymes in overwintering barley leaves may suggest the involvement of these enzymes in the tolerance mechanism to the various stresses that occur during winter.

• Molecular Medicine Reports
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• 제19권3호
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• pp.1809-1816
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• 2019

The extract of Sappan Lignum, the heartwood of Caesalpinia sappan L., has been used in medicine to improve blood circulation. Recently, the application of microwave extraction methods has been a major focus of research into the extraction of components from natural sources. In this experiment, we compared the anti-inflammatory effects of Sappan Lignum prepared by heat-70% EtOH extraction (CSE-H-70E) and microwave-70% EtOH extraction (CSE-MW-70E). High-performance liquid chromatography analysis was used to identify the compounds in these extracts. The heat-70% EtOH and microwave-70% EtOH extracts of Sappan Lignum had different chromatograms. CSE-MW-70E significantly inhibited the protein expression of iNOS and COX-2, PGE2, TNF-α, and reduced NO and IL-1β production in macrophages exposed to LPS, whereas, only high concentrations of CSE-H-70E (20 ㎍/ml) resulted in any effects. Furthermore, CSE-MW-70E upregulated heme oxygenase-1 (HO-1) expression. In addition, the use of tin protoporphyrin, an inhibitor of HO-1, confirmed the inhibitory effects of CSE-MW-70E on pro-inflammatory mediators. These results suggested that the CSE-MW-70E-mediated upregulation of HO-1 played an important role in the anti-inflammatory effects of macrophages. Therefore, these findings showed that microwave extraction can be utilized to improve the extraction efficiency and biological activity of Sappan Lignum.

• Asian-Australasian Journal of Animal Sciences
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• 제1권2호
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• pp.99-106
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• 1988

A high performance liquid chromatographic procedure is described for the direct determination of the picomole amount of palmitoyl-Coenzyme A and stearoyl-Coenzyme A, using a stainless steel column packed with C-18 derivatized porous silica ($5{\mu}m$), an isocratic elution with a mixture of 33 mM $KH_2PO_4$/acetonitrile as a mobile phase and a UV detector. The long-chain acyl-Coenzyme A esters were determined in incubated microsomal fractions of a bovine liver to demonstrate the utility of this method for monitoring acyl-CoA synthesis in biological samples. The reaction rate of palmitate was higher than that of stearate. After a 60 minute incubation period, the generated amount of palmitoyl-Coenzyme A and stearoyl-Coenzyme A were approximately 70 and 20 n mol/mg micresomal protein, respectively. The advantage of this method are in that no decomposition of the CoA esters is involved, while the constituent molecular species is detected.

• 정보처리학회논문지D
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• 제10D권6호
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• pp.897-906
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• 2003

전통적인 동물 계통수(系統樹)는 초기발생 혈질에 기초하여 몸 구조가 단순한 것에서 복잡한 것으로 동물문(animal phylum)들을 배열하는 것이다. 현재 활발하게 연구 진행되는 분자수준에서의 분자계통 분류학(Molecular Systematics) 연구들이 이런 경향을 재평가하고 새로운 계통과 진화의 의미를 제시하고 있다. 본 논문에서는 한 염기서열로부터 획득할 수 있는 특성 값들을 추출하여 트레이닝 데이터를 생성하고, 생성된 데이터를 기반으로 데이터마이닝 기법중의 하나인 분류기법(classification) 을 사용하여 계통수를 생성하였다. 실험용 데이터는 미토콘드리아 염기서열을 사용하였으며 생물학분야에서 사용하는 분석 프로그램인 MEGA 프로그램을 사용하여 이를 증명하였다. 비록 마이닝을 수행한 결과는 생물학적 실험을 거쳐 정확성을 검증 받아야 하지만 인터넷상에 떠다니는 무수한 유전체들에 대한 유효한 분류기준을 제시할 수 있고 계통수 제작을 위한 실험에 소요되는 많은 시간과 노력들을 줄일 수 있다.

• Molecules and Cells
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• 제24권3호
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• pp.307-315
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• 2007

Gene regulation is a fundamental process in biological systems, where transcription factors (TFs) play crucial roles. Inferring transcriptional interactions between TFs and their target genes has utmost importance for understanding the complex regulatory mechanisms in cellular systems. On one hand, with the rapid progress of various high-throughput experiment techniques, more and more biological data become available, which makes it possible to quantitatively study gene regulation in a systematic manner. On the other hand, transcription regulation is a complex biological process mediated by many events such as post-translational modifications, degradation, and competitive binding of multiple TFs. In this review, with a particular emphasis on computational methods, we report the recent advances of the research topics related to transcriptional regulatory networks, including how to infer transcriptional interactions, reveal combinatorial regulation mechanisms, and reconstruct TF activity profiles.

• 대한한방내과학회지
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• 제23권2호
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• pp.202-211
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• 2002

1. Background The previous studies on anticancer medicine derived from korean traditional medicine have focused on the life elongation of cancer cell bearing animals. However, it is thought that more molecular biological studies are needed to reveal their mechanism. 2. Objective The aim of this study was to investigate the molecular biological function of Sagunjatang plus Cremastrae Appenediculatae Tuber on cytostaticity, apoptosis and apoptosis related genes revelation against human stomach cancer cells(AGS). 3. Methods After administrating Sagunjatang and Sagunjatang plus Cremastrae Appenediculatae Tuber to human stomach cancer cell. MTT assay was performed to compare and examine the efficacy of each medicine on the cytostaticity of stomach cancer cells in proportion to time and doses, and apoptosis assay was performed to examine their effect on apoptosis by using DAPI dye and counting the number of cells which developed in an apoptotic body. In addition, the quantitative RT-PCR was used to examine their effect on the revelation of Bcl-2, Bax and P53, which are genes related to apoptosis. 4. Result and Conclusion Sagunjatang plus Cremastrae Appenediculatae Tuber demonstrated increased cytostaticity. decreased apoptosis and unremarkable revelation of apoptosis related genes. But in the cytostaticity and apoptosis, Sagunjatang plus Cremastrae Appenediculatae Tuber showed a tendency to control stomach cancer cells. Therefore, we can expect the clinical application to the related diseases. Besides, it needs another experiment on various cancer cells, such as, lung cancer cell and hysterocarcinoma cell.