• 제목/요약/키워드: modified shake flask method

검색결과 2건 처리시간 0.014초

Shake Flask Method와 개량 Shake Flask Method에 의한 키토산의 MRSA 향균성 평가 (A Study on the Antibacterial Activity of Chitosan on the MRSA by the Shake Flask Method and Modified Shake Flask Method)

  • 최정임;전동원
    • 한국의류산업학회지
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    • 제5권1호
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    • pp.64-70
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    • 2003
  • Water-insoluble chitosan with molecular weight of 2,000,000, 580,000, 80,000, and 40,000 and more than 90% of degree of deacetylation were prepared to test antibacterial activity of chitosan against a pathogenic bacteria, methicillin resistant Staphylococcus aureus (MRSA). As experimental method, the Shake Flask Method (SFM) and Modified Shake Flask Method (MSFM) were applicated. The anti-microbial activity of chitosan/acetic acid aqueous solution is consistent irrespective of Mw of chitosan. MIC value of SFM measurement was 0.2 ppm, and MIC value of modified SFM measurement was 25 ppm. But MIC value of chitosan/acetic add solution and chitosan treated cotton filter paper was equally 5 ppm. The antibacterial activities of chitosan were different in different test measurements employed. The antibacterial activities of chitosan/acetic acid solution and chitosan treated cotton filter paper were also different. Therefore, it needs to be pointed out that the test measurements of anti-microbial activity have some problems.

A Study on the Antibacterial Activity of Chitosan

  • Chli, In-Ryu;Park, Jeong-Im
    • 복식문화학회:학술대회논문집
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    • 복식문화학회 2003년도 International Exhibition and Workshop
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    • pp.24-25
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    • 2003
  • Water-soluble chitosan and water-insoluble chitosan with molecular weight of 2,000,000, 500,000, 80,000, and 40,000 and more than 90% degree of deacetylation were controlled to evaluate the antibacterial activity of chitosan against a pathogenic bacteria, methicillin resistant Staphylococcus aureus(MRSA), which is being issued in the world. The Shake Flask Method and Modified Shake Flask Method were used to find out the antibacterial activities of 5types of chitosan/acetic acid solution, and the other antibacterial activities test with the cotton filter treated with chitosan /acetic acid solution. Those test methods showed the great differences ,but the results of the antibacterial activites showed the same difference.

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