• Title/Summary/Keyword: model structural stability

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Biochemical and NMR Characterization of MTH1880 Mutant Proteins for Folding-Unfolding Studies

  • Kim, Hee-Youn;Ryu, Soo-Young;Yun, Ji-Hye;Kim, Suhk-Mann;Chang, Ik-Soo;Lee, Weon-Tae
    • Bulletin of the Korean Chemical Society
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    • v.31 no.12
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    • pp.3521-3524
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    • 2010
  • MTH1880 is a hypothetical protein derived from Methanobacterium thermoautotrophicum, thermophilic methanogen. The solution structure determined by NMR spectroscopy showed that it has a novel $\alpha+\beta$-fold with a highly acidic ligand binding pocket. Since MTH1880 maintains its ultra-stable structural characteristics at both high temperature and pressure, it has been considered as an excellent model for studying protein folding. To initiate the structural and folding study of MTH1880 in proving its unusual stability, we performed the site directed mutagenesis and biochemical analysis of MTH1880 mutants. Data from circular dichroism and NMR spectroscopy suggest that the point mutations perturbed the structural stability of protein even though the secondary structure is retained. This study will provide the useful information in understanding the role of participating residues during folding-unfolding process and our result will be used in designing further folding experiments for hyper-thermopile proteins like MTH1880.

Effect of countermeasures on the galloping instability of a long-span suspension footbridge

  • Ma, Ruwei;Zhou, Qiang;Li, Mingshui
    • Wind and Structures
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    • v.30 no.5
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    • pp.499-509
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    • 2020
  • The aeroelastic stability of a long-span suspension footbridge with a bluff deck (prototype section) was examined through static and dynamic wind tunnel tests using a 1:10 scale sectional model of the main girder, and the corresponding aerodynamic countermeasures were proposed in order to improve the stability. First, dynamic tests of the prototype sectional model in vertical and torsional motions were carried out at three attack angles (α = 3°, 0°, -3°). The results show that the galloping instability of the sectional model occurs at α = 3° and 0°, an observation that has never been made before. Then, the various aerodynamic countermeasures were examined through the dynamic model tests. It was found that the openings set on the vertical web of the prototype section (web-opening section) mitigate the galloping completely for all three attack angles. Finally, static tests of both the prototype and web-opening sectional models were performed to obtain the aerodynamic coefficients, which were further used to investigate the galloping mechanism by applying the Den Hartog criterion. The total damping of the prototype and web-opening models were obtained with consideration of the structural and aerodynamic damping. The total damping of the prototype model was negative for α = 0° to 7°, with the minimum value being -1.07%, suggesting the occurrence of galloping, while that of the web-opening model was positive for all investigated attack angles of α = -12° to 12°.

Experimental Study of System Identification for Seismic Response of Building Structure (건축구조물의 지진응답제어를 위한 시스템 식별의 실험적 연구)

  • 주석준;박지훈;민경원;홍성목
    • Journal of the Earthquake Engineering Society of Korea
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    • v.3 no.4
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    • pp.47-60
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    • 1999
  • The stability and efficiency of structural control systems depend on the accuracy of mathematical model of the system to be controlled. In this study, state equation models of a small scale test structure and an AMD(active mass damper) are obtained separately using OKID(observer/Kalman filter identification) which is a time domain system identification method. The test structure with each floor acceleration as outputs is identified for two inputs - the ground acceleration and the acceleration of the moving mass of AMD relative to the installation floor - individually and the two identified state equation models are integrated into one by model reduction method. The AMD is identified with the motor control signal as an input and the relative acceleration of the moving mass as an output, and it is shown that the identified model has large damping ratio and phase shift. The transfer functions and the time histories reconstructed from the identified models of the test model and the AMD match well with those measured from the experiment.

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Analysis and Design of 750kW Nacelle Cover (750kW 나셀커버 구조해석 및 설계)

  • Park, Jae-Hyun;Bang, Jo-Hyug;Park, Jin-Il;Ryu, Ji-Yune
    • 한국신재생에너지학회:학술대회논문집
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    • 2008.10a
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    • pp.295-298
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    • 2008
  • The major function of the nacelle cover is protecting the inside equipments. Therefore, it is required not only sufficient strength and stability but also light weight. In this paper, design loads are calculated according to the GL Wind guideline Ed. 2003. To ensure the structural safety, a composite structure is selected. The structural design is processed by two steps which are preliminary design and detail design. In the preliminary design step, a structural analysis is performed with initial thickness, 5mm. As reviewing above analysis results, weak regions of the nacelle cover reinforced using the spar cap structure which is same as the blade structure. In the analysis model, the support structure is connected with the nacelle cover and analyzed its structural safety at the same time.

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Structural Safety Evaluation of Yangjindang in Sang-ju Using Vibration Characteristics (동적 특성을 고려한 상주 양진당의 구조 안전성 평가)

  • Lee, Ga-Yoon;Lee, Sung-Min;Kim, Si-Yun;Lee, Kihak
    • Journal of Korean Association for Spatial Structures
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    • v.19 no.1
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    • pp.37-44
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    • 2019
  • Yangjindang house, which is located in Sang-ju province of South Korea, is one of the special Hanok structures dated back to Joseon dynasty. This study aims to examine structural safety of the Yangjindang wood frame building considering dynamic parameters such as the natural frequency and damping ratio. The numerical model of the wood frame building is implemented using Midas Gen, especially the wood joint where column and beam were connected. The behavior of the actual frame building was compared with the modeling results. In addition, structure responses such as shear force, axial force, flexural moment and deflections were calculated and compared with the allowable limits. Numerical results show that, generally, despite of some local members shear failure, Yangjindang's structural response does not exceed the limitation according to current standards.

A Study on the Characteristics of Dynamic Behaviors for the Spatial Structures using Equivalent Lumped Mass Model (중간 면진층을 가지는 래티스 돔 구조물의 병렬 다질점계 등가모델을 이용한 동적 거동 특성에 관한 연구)

  • Han Sang-Eul;Lee Sang-Ju;Kim Min-Sik
    • Journal of the Computational Structural Engineering Institute of Korea
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    • v.19 no.2 s.72
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    • pp.187-194
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    • 2006
  • Generally, earthquake-resistant structural systems have to ensure the sufficient stiffness and ductility for stability In this paper, the spatial structures are applied an isolation system to boundary parts between roof systems and sub-structures. So, it is necessary to examine the characteristics of dynamic behaviors of spatial structures governed by higher modes rather than lower modes different from the cases of high-rise buildings. The objectives of this paper are to develop the equivalent lumped mass model to simplify an analytical processes and to investigate the dynamic behaviors of roof systems according to the mass and stiffness of sub-structures as a fundamental study of performance design for the spatial structures.

The Empirical Study of Variation of KOSPI Index & Macro Economic Variation (거시경제 변수 변화와 KOSPI 지수 변동의 연관성 분석)

  • An, Chang-Ho;Choi, Chang-Yeoul
    • International Commerce and Information Review
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    • v.12 no.4
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    • pp.171-192
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    • 2010
  • In general, a stock index and its individual stocks are assumed to follow a random walk. A stock index is an important source of information and one that is seen by people everyday, regardless of their investment intentions. This paper examines the correlation between the KOSPI-the index that best reflects the Korean stock market and the macro - economic variables that have been found to influence the index by previous studies. The sample period considers the years after 2000 when the Korean stock market matured as restrictions on foreign investors were removed. For this purpose, a Vector Error Correction Model (VECM) and KOSPI equation with a general pacific approach were used. This paper aims at verifying the factors that determined the KOSPI after 2000 and at examining whether there was structural change in the investment environment. It also investigates changes in the factors determining the KOSPI's performance as a result of structural changes in the investment environment. The V AR (Vector Autoregressive) model including the nine variables was selected as a baseline model whose stability was tested using the unit root test. The results from the VECM and the structural changes in the investment environment can be summarized by the following Inner story points.

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A Large-scale Structural Mixing Model applied to Blowout of Turbulent Nonpremixed Jet Flames in a Cross Jet Flow (횡분류(流)(橫噴流)에서 난류 비예흔합 화염의 화염날림에 대한 거대 와(渦)구조 혼합 모텔 적용)

  • Lee, Kee-Man;Park, Jeong
    • Transactions of the Korean Society of Mechanical Engineers B
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    • v.26 no.1
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    • pp.133-140
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    • 2002
  • This article presents an application of a large-scale structural mixing model(Broadwell et at. 1984) to the blowout of turbulent reacting cross flow jets. Experimental observations, therefore, aim to identify the existence of large-scale vortical structure exerting an important effect upon the flame stabilization. In the analysis of common stability curve, it is seen that the phenomenon of blowout are only related to the mixing time scale of the two flows. The most notable observation is that the blowout distance is traced at a fixed positions according to the velocity ratio at all times. Measurements of the lower blowout limits in the liftable flame are qualitatively in agreement with the blowout parameter $\xi$, proposed by Broadwell et al. Good agrement between the results calculated by a modified blowout parameter $\xi$'and the present experimental results confirms the important effect of large-scale structure in the stabilization feature of blowout.

Enhanced finite element modeling for geometric non-linear analysis of cable-supported structures

  • Song, Myung-Kwan;Kim, Sun-Hoon;Choi, Chang-Koon
    • Structural Engineering and Mechanics
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    • v.22 no.5
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    • pp.575-597
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    • 2006
  • Enhanced three-dimensional finite elements for geometrically nonlinear analysis of cable-supported structures are presented. The cable element, derived by using the concept of an equivalent modulus of elasticity and assuming the deflection curve of a cable as catenary function, is proposed to model the cables. The stability functions for a frame member are modified to obtain a numerically stable solution. Various numerical examples are solved to illustrate the versatility and efficiency of the proposed finite element model. It is shown that the finite elements proposed in this study can be very useful for geometrically nonlinear analysis as well as free vibration analysis of three-dimensional cable-supported structures.

Effects of damping on the linear stability of a free-free beam subjected to follower and transversal forces

  • Kavianipour, O.;Sadati, S.H.
    • Structural Engineering and Mechanics
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    • v.33 no.6
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    • pp.709-724
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    • 2009
  • In this paper a free-free uniform beam with damping effects subjected to follower and transversal forces at its end is considered as a model for a space structure. The effect of damping on the stability of the system is first investigated and the effects of the follower and transversal forces on the vibration of the beam are shown next. Proportional damping model is used in this work, hence, the effects of both internal (material) and external (viscous fluid) damping on the system are noted. In order to derive the frequency of the system, the Ritz method has been used. The mode shapes of the system must therefore be extracted. The Newmark method is utilized in the study of the system vibration. The results show that an increase in the follower and transversal forces leads to an increase of the vibrational motion of the beam which is not desirable.