• Journal of Microbiology and Biotechnology
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• v.14 no.6
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• pp.1239-1248
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• 2004

The methylotrophic yeast Hansenula polymorpha has been extensively studied as a model organism for methanol metabolism and peroxisome biogenesis. Recently, this yeast has also attracted attention as a promising host organism for recombinant protein production. Here, we describe the fabrication and evaluation of a DNA chip spotted with 382 open reading frames (ORFs) of H. polymorpha. Each ORF was PCR-amplified using gene-specific primer sets, of which the forward primers had 5'-aminolink. The PCR products were printed in duplicate onto the aldehyde-coated slide glasses to link only the coding strands to the surface of the slide via covalent coupling between amine and aldehyde groups. With the partial genome DNA chip, we compared efficiency of direct and indirect cDNA target labeling methods, and found that the indirect method, using fluorescent-labeled dendrimers, generated a higher hybridization signal-to-noise ratio than the direct method, using cDNA targets labeled by incorporation of fluorescence-labeled nucIeotides during reverse transcription. In addition, to assess the quality of this DNA chip, we analyzed the expression profiles of H. polymorpha cells grown on different carbon sources, such as glucose and methanol, and also those of cells treated with the superoxide­generating drug, menadione. The profiles obtained showed a high-level induction of a set of ORFs involved in methanol metabolism and oxidative stress response in the presence of methanol and menadione, respectively. The results demonstrate the sensitivity and reliability of our arrays to analyze global gene expression changes of H. polymorpha under defined environmental conditions.

• Journal of Korean Society of Environmental Engineers
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• v.39 no.8
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• pp.478-488
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• 2017

Stormwater filtration is widely used for the urban runoff treatment. However, intensive maintenance and lack of information about the performance have resulted in an increased need of proper evaluation. In this study, the performance of an upflow stormwater runoff filtration system, consisting of a supporting unit and a filtration unit filled with a ceramic media, was investigated. The maximum head loss increase was about 3 cm under the suspended solid (SS) load of $30kg/m^2$ and the SS removal was more than 96%, when the filtration velocity was 20-40 m/h. The head loss and the porosity of the media can successfully be described by a power model. It was confirmed that the a significant amount of SS can effectively be removed at supporting unit, minimizing SS load to the filter media bed. Several backwashing strategies have been tested to establish the optimum condition. It was found that the stagnant water discharge is important to minimize the SS release immediately after backwashing. Also, the filter bed loaded with $400-450kg/m^2$ SS can almost completely be washed to reduce the head loss to the that of empty bed. The results in this study indicate that the upflow ceramic media filter is an excellent alternative to stormwater treatment, with high SS removal and long lifespan.

• Journal of Embryo Transfer
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• v.15 no.3
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• pp.247-253
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• 2000

This study was undertaken to optimize enucleation and reconstitution methods for the production of cloned mice by somatic cell nuclear transfer Outbred ICR mouse oocytes at the metapahse- II stage were retrieved from female mice superovulated by PMSG and hCG. In Experiment 1, oocytes were enucleated in medium supplemented with cytochalasin B (CCB) of 3 levels (0, 7.5 or 15 $\mu\textrm{g}$/mL), and higher rate of encleation was obtained at 7.5 and 15 $\mu\textrm{g}$/mL than at $\mu\textrm{g}$/mL. In Experiment 2, oocytes enucleated in 7.5 $\mu\textrm{g}$/mL CCB-containing medium were reconstituted with different types of somatic cell by following methods; 1) cumulus cells by direct cell injection, 2) cumulus cells by electric fusion (1.25 kV/cm, 2 pulses for each 70 $mutextrm{s}$) or 3) STO cells by the electrofusion. Electrofusion of STO cells with enucleated oocytes yielded the greatest (P<0.05) rate of reconstitution without lysis (76%) than any other combinations. Although significant decrease in the rate of somatic cell introduction was found, the electrofusion of cumulus cells yielded better rate of reconstitution than direct injection (0 vs. 18%). In Experiment 3, the duration of electric stimulation for the fusion was changed to either 50 $mutextrm{s}$ or 90 $mutextrm{s}$, but no significant improvement of reconstitution efficacy was obtained. In conclusion, this study showed that ICR mouse oocytes could be used for the production of reconstituted oocytes and a fusion method of 1.25 KV/cm with 2 pulses using 570 cell was the optimal.

• Journal of Korean Tunnelling and Underground Space Association
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• v.17 no.3
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• pp.257-266
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• 2015

This study introduce that we studied optimization and possibility of smoke spread prevention with air-curtain system in undersea tunnel named from Ho-Nam to Jeju line in domestic if a fire break out in train. To verify performance, air-curtain system is installed between rescue station platform and each door of passenger car to provide safety route to evacuator and we studied simulation model of various cases about 15 MW fire severity considering domestic specifications. As a result we verified the fact that CASE1(air jet with 15degree toward passenger car) and CASE 5 (air jet with 15degree toward passenger car and pressure air blast from cross passage) is best Smoke Spread Prevention and less inflow carbon monoxide. Through above results, we expect that air-curtain system is one of the facilities for fire safety and provide us safety platform route in undersea tunnel.

• Environmental and Resource Economics Review
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• v.15 no.4
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• pp.693-712
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• 2006

A pre-feasibility test is done for renewable energy hybrid power systems at off~grid islands in which the current power supply is provided only by diesel generation. We apply Homer (Hybrid Optimization Model for Electric Renewables) which was developed by the National Renewable Energy Laboratory (NREL) for the analysis to identify the cost-minimizing combination of power generating facilities for the given load profiles. Chuja-Do, Geomun-Do and Youngsan-Do have been selected for our analysis considering the wind resources data of the Korea Institute of Energy Research (KIER). Information on wind speed, solar radiation and temperature is also used for the analysis. System component cost information from overseas market has been used due to the lack of domestic information. Site specific Load profile for electricity demand for those islands are reconstructed based on the partial survey results obtained form other sources. The LCOE of the least cost hybrid power systems for Chuja-Do, Geomun-Do and Youngsan-Do are $0.278/kWh, $0.234/kWh and $0.353/kWh, respectively Considering the fact that diesel generation is being subsidized at the price of $0.300/kWh by the government, first 2 cases are economically feasible for the introduction of renewable energy hybrid systems to those islands. But the third case of Youngsan-Do does not meet the criteria. The basic differences of these pre-feasibility test results are from the differences of the site specific renewable energy conditions, especially wind resources. In summary, promoting hybrid systems in the off-grid remote island should be based on the economic feasibility test results. Not all the off-grid islands are feasible for introducing this renewable energy hybrid system.

• Current Research on Agriculture and Life Sciences
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• v.6
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• pp.163-169
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• 1988

This study was conducted to predict the density changes according to concentration and temperature changes under freezing point. This information is needed for the design of freezing equipment and for the efficient utilization of refrigerating system. Orange juice, Apple juice, Grape juice and Sucrose solution were used for the measurement of density in this study at the temperature range from $-5^{\circ}C$ to $-40^{\circ}C$ and at the concentration range from 10 to 40%. The unfrozen water fraction of samples was determined by Heldman's method. The density values were determined by measuring the weight of a frozen solution at each temperature with a known volume. Solutions were placed in the thick-walled aluminum tubes. When the solution was frozen the excess ice was removed with a razor until the surface of the ice was flush with the top of the aluminum tube. The tube and ice were weighted immediately. Knowing the volume, tare weight, and final weight, the density could be determined. With this procedure, the data of density and unfrozen water fraction for fruit juices and sucrose solution were collected. The density prediction models of fruit juices and sucrose solution under freezing point were established by the optimization computer program with measured experimental data.

• Journal of Biomedical Engineering Research
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• v.38 no.3
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• pp.116-122
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• 2017

Physical breast phantoms would be useful for the development of a dedicated breast computed tomography (BCT) system and its optimization. While the conventional breast phantoms are available in compressed forms, which are appropriate for the mammography and digital tomosynthesis, however, the BCT requires phantoms in uncompressed forms. Although simple cylindrical plastic phantoms can be used for the development of the BCT system, they will not replace the roles of uncompressed phantoms describing breast anatomies for a better study of the BCT. In this study, we have designed a numerical voxel breast phantom accounting for the random nature of breast anatomies and applied it to the 3D printer to fabricate the uncompressed anthropomorphic breast phantom. The numerical voxel phantom mainly consists of the external skin and internal anatomies, including the ductal networks, the glandular tissues, the Cooper's ligaments, and the adipose tissues. The voxel phantom is then converted into a surface data in the STL file format by using the marching cube algorithm. Using the STL file, we obtain the skin and the glandular tissue from the 3D printer, and then assemble them. The uncompressed breast phantom is completed by filling the remaining space with oil, which mimics the adipose tissues. Since the breast phantom developed in this study is completely software-generated, we can create readily anthropomorphic phantoms accounting for diverse human breast anatomies.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.18 no.1
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• pp.579-584
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• 2017

The damper oil seal of a high-speed railway vehicle is made from nitrile butadiene rubber (NBR) in order to prevent lubricant from leaking into the damper and to stop harmful contaminants from entering the external environment while in service. Oil leakage through the seal primarily occurs from fatigue failure of the damper. Cumulative damage of the seal occurs due to the contact force between the rod and the rubber during movement due to track irregularities and cants, among other factors. Thus, the design of the oil seal should minimize the maximum principal strain at weak points. In this study, the optimal cross section of the damper oil seal was found using the multi-island genetic algorithm method to improve the durability of the damper. The optimal shape of the oil seal was derived using process automation and design optimization software. Nonlinear material properties for finite element analysis (FEA) of the rubber were determined by Marlow's model. The nonlinear FEA confirmed that the maximum principal strain at the oil leakage point was decreased 24% between the initial design and the optimum design.

• Journal of KIISE:Computer Systems and Theory
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• v.35 no.9_10
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• pp.485-496
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• 2008

As the complexity of SoC (System-on-Chip) design increases dramatically. traditional system performance analysis and verification methods based on RTL (Register Transfer Level) are no more valid for increasing time-to-market pressure. Therefore a new design methodology is desperately required for system verification in early design stages. and hardware software (HW-SW) cosimulation at TLM (Transaction Level Modeling) level has been researched widely for solving this problem. However, most of HW-SW cosimulators support few restricted ion levels only, which makes it difficult to integrate HW-SW cosimulators with different ion levels. To overcome this difficulty, this paper proposes a multipurpose framework for HW SW cosimulation to provide systematic SoC design flow starting from software application design. It supports various design techniques flexibly for each design step, and various HW-SW cosimulators. Since a platform design is possible independently of ion levels and description languages, it allows us to generate simulation models with various ion levels. We verified the proposed framework to model a commercial SoC platform based on an ARM9 processor. It was also proved that this framework could be used for the performance optimization of an MJPEG example up to 44% successfully.

• Journal of Navigation and Port Research
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• v.41 no.3
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• pp.137-148
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• 2017

Port service level is a metric of competitiveness among ports for the operating/managing bodies such as the terminal operation company (TOC), Port Authority, or the government, and is used as an important indicator for shipping companies and freight haulers when selecting a port. Considering the importance of metrics, we developed software to objectively define and manage six important service indicators exclusive to container and bulk terminals including: berth occupancy rate, ship's waiting ratio, berth throughput, number of berths, average number of vessels waiting, and average waiting time. We computed the six service indicators utilizing berth 1 through berth 5 in the container terminals and berth 1 through berth 4 in the bulk terminals. The software model allows easy computation of expected ship's waiting ratio over berth occupancy rate, berth throughput, counts of berth, average number of vessels waiting and average waiting time. Further, the software allows prediction of yearly throughput by utilizing a ship's waiting ratio and other productivity indicators and making calculations based on arrival patterns of ship traffic. As a result, a TOC is able to make strategic decisions on the trade-offs in the optimal operating level of the facility with better predictors of the service factors (ship's waiting ratio) and productivity factors (yearly throughput). Successful implementation of the software would attract more shipping companies and shippers and maximize TOC profits.