• 제목/요약/키워드: mixture experimental design

검색결과 300건 처리시간 0.024초

Effects of Eucalyptus Crude Oils Supplementation on Rumen Fermentation, Microorganism and Nutrient Digestibility in Swamp Buffaloes

  • Thao, N.T.;Wanapat, M.;Cherdthong, A.;Kang, S.
    • Asian-Australasian Journal of Animal Sciences
    • /
    • 제27권1호
    • /
    • pp.46-54
    • /
    • 2014
  • This study was conducted to investigate the effects of eucalyptus (E. Camaldulensis) crude oils (EuO) supplementation on voluntary feed intake and rumen fermentation characteristics in swamp buffaloes. Four rumen fistulated swamp buffaloes, body weight (BW) of $420{\pm}15.0$ kg, were randomly assigned according to a $2{\times}2$ factorial arrangement in a $4{\times}4$ Latin square design. The dietary treatments were untreated rice straw (RS) without EuO (T1) and with EuO (T2) supplementation, and 3% urea-treated rice straw (UTRS) without EuO (T3) and with EuO (T4) supplementation. The EuO was supplemented at 2 mL/h/d in respective treatment. Experimental animals were kept in individual pens and concentrate mixture was offered at 3 g/kg BW while roughage was fed ad libitum. Total dry matter and roughage intake, and apparent digestibilites of organic matter and neutral detergent fiber were improved (p<0.01) by UTRS. There was no effect of EuO supplementation on feed intake and nutrient digestibility. Ruminal pH and temperature were not (p>0.05) affected by either roughage sources or EuO supplementation. However, buffaloes fed UTRS had higher ruminal ammonia nitrogen and blood urea nitrogen as compared with RS. Total volatile fatty acid and butyrate proportion were similar among treatments, whereas acetate was decreased and propionate molar proportion was increased by EuO supplementation. Feeding UTRS resulted in lower acetate and higher propionate concentration compared to RS. Moreover, supplementation of EuO reduced methane production especially in UTRS treatment. Protozoa populations were reduced by EuO supplementation while fungi zoospores remained the same. Total, amylolytic and cellulolytic bacterial populations were increased (p<0.01) by UTRS; However, EuO supplementation did not affect viable bacteria. Nitrogen intake and in feces were found higher in buffaloes fed UTRS. A positive nitrogen balance (absorption and retention) was in buffaloes fed UTRS. Supplementation of EuO did not affect nitrogen utilization. Both allantoin excretion and absorption and microbial nitrogen supply were increased by UTRS whereas efficiency of microbial protein synthesis was similar in all treatments. Findings of present study suggested that EuO could be used as a feed additive to modify the rumen fermentation in reducing methane production both in RS and UTRS. Feeding UTRS could improve feed intake and efficiency of rumen fermentation in swamp buffaloes. However, more research is warranted to determine the effect of EuO supplementation in production animals.

Effects of dietary enzyme cocktail on growth performance, intestinal morphology, and nutrient digestibility of weaned pigs

  • Kim, Yunkang;Baek, Jangryeol;Jang, Kibeom;Kim, Junsu;Kim, Sheena;Mun, Daye;Kim, Byeonghyeon;Kim, Younghwa;Park, Juncheol;Choe, Jeehwan;Song, Minho
    • 농업과학연구
    • /
    • 제44권4호
    • /
    • pp.513-518
    • /
    • 2017
  • Soybean, one of most widely used swine feed component in the world, contains non-starch polysaccharides (NSP). The digestive system of weaned pigs is not yet fully developed, and thus weaned pigs cannot easily digest diets based on corn and soybean meal. Dietary exogenous enzymes supplementation has been intensively investigated to assist digestion of anti-nutritional factors, such as NSP. This experiment was conducted to investigate the effects of dietary enzyme cocktail on growth performance, intestinal morphology, and nutrient digestibility of weaned pigs. A total 36 weaned pigs ($5.92{\pm}0.48kg\;BW$; 28 d old) were randomly allotted to 2 dietary treatments (3 pigs/pen, 6 replicates/treatment) in a randomized complete block design. The dietary treatments were a typical diet based on corn and soybean meal (CON) and CON with 0.05% enzyme cocktail (Cocktail; mixture of xylanase, ${\alpha}-amylase$, protease, ${\beta}-glucanase$, and pectinase). Pigs were fed their respective diets for 6 wk. Growth performance, morphology of ileum, apparent ileal digestibility (AID) and apparent total tract digestibility (ATTD) of dry matter, crude protein, and energy of weaned pigs were measured. No significant differences (p > 0.05) were observed for growth performance for the duration of the experimental period, and morphology of ileum, and nutrient digestibility between CON and Cocktail treatment groups. Therefore, the results from the current study indicated that enzyme cocktail supplementation in diets had no influence on growth performance, intestinal morphology, and nutrient digestibility of weaned pigs.

수정 fluid loss 시험을 이용한 연직 차수벽에 생성된 벤토나이트 필터케익 특성 평가 (Characteristics of Bentonite Filter Cake on Vertical Cutoff Walls Evaluated by Modified Fluid Loss Test)

  • 웽테바오;박문석;임지희;최항석;한신인
    • 한국지반공학회논문집
    • /
    • 제27권2호
    • /
    • pp.53-62
    • /
    • 2011
  • 연직 차수벽 시공 시, 벤토나이트 슬러리는 토양층으로 여과되면서 차수벽의 측벽 표면에 필터케익 층을 형성하고 이렇게 형성된 필터케익은 차수벽의 자체 투수계수보다 매우 낮은 값을 갖는다. 본 연구에서는 수정 fluid loss 시험을 수행하여 다양한 작용압력 하에서 슬러리월 시공현장에서 주로 사용되는 세 가지 종류의 벤토나이트로 형성된 필터케익의 투수계수를 평가하였다. 수정 fluid loss 시험에서는 일반적인 연직 차수벽 시공 조건을 반영하기 위해 중량비 4, 6, 8% 농도의 벤토나이트 슬러리를 적용하였다. 벤토나이트 필터케익의 투수계수를 예측하기 위해 수정 fluid loss 시험 결과를 기존에 제안된 두 가지 방법을 이용하여 해석하였다. 본 연구결과로부터 평가된 세 가지 벤토나이트 필터케익의 투수계수는 $2.15{\times}10^{-11}m/s$$2.88{\times}10^{-10}m/s$ 범위로 이는 일반적인 연직차수벽 뒤채움재의 설계값 보다 10-1000배 가량 작음을 알 수 있다. 또한, 필터케익 내 응력분포와 필터케익의 두께가 각 조건에 대해 비교되었다.

Nutrient Digestibility, Ruminal Fermentation Activities, Serum Parameters and Milk Production and Composition of Lactating Goats Fed Diets Containing Rice Straw Treated with Pleurotus ostreatus

  • Kholif, A.E.;Khattab, H.M.;El-Shewy, A.A.;Salem, A.Z.M.;Kholif, A.M.;El-Sayed, M.M.;Gado, H.M.;Mariezcurrena, M.D.
    • Asian-Australasian Journal of Animal Sciences
    • /
    • 제27권3호
    • /
    • pp.357-364
    • /
    • 2014
  • The study evaluated replacement of Egyptian berseem clover (BC, Trifolium alexandrinum) with spent rice straw (SRS) of Pleurotus ostreatus basidiomycete in diets of lactating Baladi goats. Nine lactating homo-parity Baladi goats (average BW $23.8{\pm}0.4$ kg) at 7 d postpartum were used in a triplicate $3{\times}3$ Latin square design with 30 d experimental periods. Goats were fed a basal diet containing 0 (Control), 0.25 (SRS25) and 0.45 (SRS45) (w/w, DM basis) of SRS. The Control diet was berseem clover and concentrate mixture (1:1 DM basis). The SRS45 had lowered total feed intake and forages intake compared to Control. The SRS25 and SRS45 rations had the highest digestibilities of DM (p = 0.0241) and hemicellulose (p = 0.0021) compared to Control which had higher (p<0.01) digestibilities of OM (p = 0.0002) and CP (p = 0.0005) than SRS25 and SRS45. Ruminal pH and microbial protein synthesis were higher (p<0.0001) for SRS25 and SRS45 than Control, which also had the highest (p<0.0001) concentration of TVFA, total proteins, non-protein N, and ammonia-N. All values of serum constituents were within normal ranges. The Control ration had higher serum globulin (p = 0.0148), creatinine (p = 0.0150), glucose (p = 0.0002) and cholesterol (p = 0.0016). Both Control and SRS25 groups had the highest (p<0.05) milk (p = 0.0330) and energy corrected milk (p = 0.0290) yields. Fat content was higher (p = 0.0373) with SRS45 and SRS25 groups compared with Control. Replacement of BC with SRS in goat rations increased milk levels of conjugated linoleic acid and unsaturated fatty acids compared with Control. It was concluded that replacing 50% of Egyptian berseem clover with SRS in goat rations improved their productive performance without marked effects on metabolic indicators health.

한방 닭죽 개발 및 최적 배합비율 확립 (Development of Herbal Chicken Porridge and the Establishment of Optimizing the Mixing Ratio)

  • 김경연;백무열;박천석;최성원;김창남;김병용
    • 한국식품영양학회지
    • /
    • 제26권1호
    • /
    • pp.22-28
    • /
    • 2013
  • 죽의 기능성 향상을 위하여 2:1:1:1의 배합으로 혼합한 한약재료(가시오가피, 숙지황, 산약, 백복령)와 닭가슴살 등의 재료를 사용해 한방 영양죽을 제조하였다. 한방 닭죽의 DPPH radical 소거능, SOD 유사활성, 맛의 선호도, cost는 수학적인 canonical model과 trace plot을 이용하여 분석한 결과, SOD 유사활성 이외에는 각 성분들이 독립적으로 작용하여 상호작용이 없는 선형 모델을 보여주었다. DPPH radical 소거능, SOD 유사활성, 맛의 선호도를 최대로, cost를 최소로 목적 범위를 설정하고, 각각의 canonical 계수를 이용하여 수적 최적화를 통하여 최적 배합비를 구한 결과, 한약 혼합물 101 g, 닭가슴살 38.66 g, 찹쌀 비율 41.34 g의 최적 배합비를 구하였으며, 이 배합비에 따른 맛의 선호도는 6.39, DPPH radical 소거능은 17.25 mgVCE/${\ell}$, SOD 유사활성은 2.25%, cost는 1,382.39 won으로 나타났으며, 여러 결과들 사이에 최적화된 한 값에 수렴하는 desirability는 0.714로 나타났다. 이는 한약혼합물이 많을수록 기능성 면에서는 높게 나타나지만 맛의 선호도면에서는 낮게 나타냈고, 닭가슴살의 경우에도 그 함량이 많을수록 맛의 선호도는 올라갔지만 그에 반해 cost가 높게 올라간 것을 반영한 결과로 사료된다.

N-vinyl-2-pyrrolidone과 2-pyrrolidone 혼합물의 고-액 상평형 및 용융결정화를 이용한 N-vinyl-2-pyrrolidone의 결정성장속도 연구 (Studies of Solid-Liquid Phase Equilibria for Mixtures of N-vinyl-2-pyrrolidone+2-pyrrolidone and Growth Rate of N-vinyl-2-pyrrolidone Crystal Using Melt Crystallization)

  • 김선형;서명도;탁문선;김우식;양대륙;강정원
    • Korean Chemical Engineering Research
    • /
    • 제51권5호
    • /
    • pp.587-590
    • /
    • 2013
  • N-vinyl-2-pyrrolidone (NVP)에 포함된 불순물인 2-pyrrolidone을 제거하기 위해 용융결정화가 이용될 수 있으며, 그 기본 연구로써 두 물질의 고-액 상평형을 측정하였다. 시차주사 열량계(DSC)와 결정화기를 이용하여 얻어진 두 실험결과는 비슷한 경향을 보였으며, NVP와 2-pyrrolidone으로 구성된 2성분계 혼합물이 공융계를 형성함을 보였다. 간단한 열역학 식을 이용하여 혼합물의 상평형과 공융점(eutectic point)을 계산하였으며 실험결과와 비교적 잘 일치하였다. 결정화 공정의 설계에 중요한 요소인 결정성장속도를 알기 위해 판형 결정화기를 이용하여 시간에 따른 NVP 결정의 두께를 측정하였다. 냉각온도가 낮을수록 NVP의 결정성장속도가 증가하였다. 실험데이터로부터 상관된 열전달계수는 결정의 성장 거동을 잘 설명하였다.

SNR 기반 가중 KL 거리를 활용한 화자 변화 검증에 관한 연구 (The Study on Speaker Change Verification Using SNR based weighted KL distance)

  • 조준범;이지은;이경록
    • 융합정보논문지
    • /
    • 제7권6호
    • /
    • pp.159-166
    • /
    • 2017
  • 본 논문에서는 방송 뉴스에서 화자 변화 검증 성능 향상을 위해서 입력소음음성 향상과 SNR(Signal to Noise Ratio)기반 가중 함수 $w_m$를 적용한 KL 거리 $D_s$를 실험하였다. GMM-UBM(Gaussian Mixture Model-Universal Background Model) 기반 KL(Kullback Leibler) 거리 D를 이용한 화자 변화 검증 시스템(실험 0)을 기본 시스템으로 한다. 실험 1은 실험 0의 입력소음음성 향상을 위해 MMSE Log-STSA(Minimum Mean Square Error Log-Spectral Amplitude Estimator)를 적용하였다. 실험 2는 실험 1의 기존 KL거리 D 대신에 $D_s$를 적용하였다. 실험 데이터베이스는 다양한 소음을 반영하기 위해 스포츠 뉴스와 실외 인터뷰를 중심으로 구축하였다. 실험은 화자 변화 정보의 누락을 막기 위해 MDR(Missed Detection Rate) 0%를 기준으로 하였다. 실험 0은 FAR(False Alarm Rate) 71.5%의 성능을 보였다. 실험 1은 FAR 67.3%로 실험0에 비해 4.2% 향상되었고, 실험 2는 FAR 60.7%로 10.8% 향상되었다.

친환경 복합효소제 첨가가 이유자돈의 성장, 장내 형태학, 영양소 소화율에 미치는 영향 (Effects of Eco-friendly Multi-enzyme on Growth Performance, Intestinal Morphology, and Nutrient Digestibility of weaned Pigs)

  • 김성기;조명우;김준수;장기범;김신아;문다예;김병현;김영화;박준철;최지환;송민호
    • 한국유기농업학회지
    • /
    • 제26권1호
    • /
    • pp.141-149
    • /
    • 2018
  • This experiment was conducted to investigate the effects of multi-enzyme on growth performance, intestinal morphology, and nutrient digestibility of weaned pigs. A total 36 weaned pigs ($5.92{\pm}0.48kg\;BW$; 28 d old) were randomly allotted to 2 dietary treatments (3 pigs/pen, 6 replicates/treatment) in a randomized complete block design. The dietary treatments were a typical diet based on corn and soybean meal (CON) and CON with 0.1% multi-enzyme (Multi; mixture of ${\beta}-mannanase$, xylanase, ${\alpha}-amylase$, protease, ${\beta}-glucanase$, and pectinase). Pigs were fed their respective diets for 6 wk. Measurements were growth performance, morphology of ileum, apparent ileal digestibility and apparent total tract digestibility of dry matter, crude protein, and energy of weaned pigs. There were no significant differences on growth performance during overall experimental period. No differences were found for the morphology of ileum and nutrient digestibility between CON and Multi groups. Therefore, the results in the current study indicated that multi-enzyme supplementation in diets had no effects on growth performance, intestinal morphology, and nutrient digestibility of weaned pigs.

반응표면 분석법에 의한 흑마늘 추출물이 첨가된 간장의 제조 조건 설정 (Optimization of Soy Sauce Production Conditions with Black Garlic Extract by Response Surface Methodology)

  • 심혜진;강민정;김경민;이창권;김정환;신정혜
    • 한국식품조리과학회지
    • /
    • 제32권3호
    • /
    • pp.307-315
    • /
    • 2016
  • Purpose: The central composition design was used to optimize the mixture conditions of black garlic extract. Methods: The response surface methodology (RSM) was carried out from concentration of black garlic extract ($X_1$) and the amount of the black garlic extract ($X_2$) as independent variables, and salts ($Y_1$), reducing sugars ($Y_2$), the content of total phenolic compounds ($Y_3$) and ABTS radical scavenging activity ($Y_4$) as dependent variables. We confirmed the conditions that salinity was minimized and reducing sugar, total phenolic compounds and ABTS radical scavenging activity had maximum values through the response surface analysis. Results: All results had saddle points in originally set up conditions hence, ridge analysis was carried out for narrowing the experimental area. The minimum salt concentration was 16.03% at black garlic extract concentration of $14.84^{\circ}brix$ and contents of 9.26%. Reducing sugar content had maximum of value 7.30 g/mL at $24.58^{\circ}brix$ and contents of 8.08%. Total phenolic compounds contents and ABTS radical scavenging activity had maximum values at black garlic extract concentration of 20.33 and $25.02^{\circ}brix$. The results indicate that addition of black garlic extract contributed to increased reducing sugar, phenolic compounds contents and antioxidant activity of the soy sauce, but the salt concentration was not significantly affected. Conclusion: Based on the results of RSM, the optimum ranges of addition conditions for lowering the salt concentration and, increasing the sensory and functional ability of soy sauce were as follows: black garlic extract concentration of $15-25^{\circ}brix$ and content of 7.8-9.3%.

저위생산답(低位生産沓) 토양(土壤)에 대(對)한 개량제(改良劑)와 인산(燐酸)의 효과(效果) (The effect of a soil amendment on phosphate efficiency in a low productive paddy soil)

  • 심상칠;송기준;김정자
    • 한국토양비료학회지
    • /
    • 제4권1호
    • /
    • pp.21-26
    • /
    • 1971
  • 몇가지 미량원소(微量元素)(Mn, Cu, Zn, B)를 혼합(混合)한 시판(市販) 규산질비료(珪酸質肥料)의 추답토양(秋畓土壤)에 대(對)한 개량제(改良劑)로서의 효과(效果)와 인산질비료(燐酸質肥料)의 비효(肥效) 증진(增進)에 미치는 영향(影響)을 조사(調査)한 결과(結果)는 다음과 같았다. 1. 개량제(改良劑)로서의 시용(施用) 효과(效果)가 현저(顯著)하였다. 대조구(對照區)의 정조수량(精粗收量) 462kg/10a에 비하여 개량제처(改良劑處) 이구(理區)는 560kg/10a 로 이는 천립중(千粒重), 등숙비(登熟比), 임실률(稔實率) 및 수당입수(穗當粒數)가 개량제(改良劑)의 처리(處理)에 의(依)해 각각(各各) 증가(增加)한 때문인 것 같다. 2. 개량제(改良濟)의 처리(處理)에 의(依)해 인산(燐酸)의 비효(肥效)가 증대(增大)되는 경향(傾向)이었다. 인산(燐酸)의 시비량(施肥量) 증가(增加)에 의(依)해 천립중(千粒重), 임실률(稔實率), 등숙비(登熟比)가 증가(增加)되었으며 특(特)히 대조구(對照區)의 임실률(稔實率)이 크게 증가(增加)되었다. 3. 인산(燐酸)의 시용(施用)은 수도(水稻)의 생육(生育)을 촉진(促進)하고 개량제(改良劑)의 시용(施用)은 생육(生育)을 억제(抑制)하는 경향(傾向)이 었으며 결과적(結果的)으로 개량제(改良劑)의 시용(施用)에 의(依)해 무효분얼(無效分蘖)의 발생(發生)이 억제(抑制)되었다. 4. 개량제(改良劑)의 시용(施用)에 의(依)해 식물체중(植物體中)의 규산(珪酸)과 망강의 함량(含量)은 증가(增加)하고 인산(燐酸), 동(銅), 아연(亞鉛)의 함량(含量)은 감소(減少)되었다.

  • PDF