• Title/Summary/Keyword: mixture experimental design

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Effects of Eucalyptus Crude Oils Supplementation on Rumen Fermentation, Microorganism and Nutrient Digestibility in Swamp Buffaloes

  • Thao, N.T.;Wanapat, M.;Cherdthong, A.;Kang, S.
    • Asian-Australasian Journal of Animal Sciences
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    • v.27 no.1
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    • pp.46-54
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    • 2014
  • This study was conducted to investigate the effects of eucalyptus (E. Camaldulensis) crude oils (EuO) supplementation on voluntary feed intake and rumen fermentation characteristics in swamp buffaloes. Four rumen fistulated swamp buffaloes, body weight (BW) of $420{\pm}15.0$ kg, were randomly assigned according to a $2{\times}2$ factorial arrangement in a $4{\times}4$ Latin square design. The dietary treatments were untreated rice straw (RS) without EuO (T1) and with EuO (T2) supplementation, and 3% urea-treated rice straw (UTRS) without EuO (T3) and with EuO (T4) supplementation. The EuO was supplemented at 2 mL/h/d in respective treatment. Experimental animals were kept in individual pens and concentrate mixture was offered at 3 g/kg BW while roughage was fed ad libitum. Total dry matter and roughage intake, and apparent digestibilites of organic matter and neutral detergent fiber were improved (p<0.01) by UTRS. There was no effect of EuO supplementation on feed intake and nutrient digestibility. Ruminal pH and temperature were not (p>0.05) affected by either roughage sources or EuO supplementation. However, buffaloes fed UTRS had higher ruminal ammonia nitrogen and blood urea nitrogen as compared with RS. Total volatile fatty acid and butyrate proportion were similar among treatments, whereas acetate was decreased and propionate molar proportion was increased by EuO supplementation. Feeding UTRS resulted in lower acetate and higher propionate concentration compared to RS. Moreover, supplementation of EuO reduced methane production especially in UTRS treatment. Protozoa populations were reduced by EuO supplementation while fungi zoospores remained the same. Total, amylolytic and cellulolytic bacterial populations were increased (p<0.01) by UTRS; However, EuO supplementation did not affect viable bacteria. Nitrogen intake and in feces were found higher in buffaloes fed UTRS. A positive nitrogen balance (absorption and retention) was in buffaloes fed UTRS. Supplementation of EuO did not affect nitrogen utilization. Both allantoin excretion and absorption and microbial nitrogen supply were increased by UTRS whereas efficiency of microbial protein synthesis was similar in all treatments. Findings of present study suggested that EuO could be used as a feed additive to modify the rumen fermentation in reducing methane production both in RS and UTRS. Feeding UTRS could improve feed intake and efficiency of rumen fermentation in swamp buffaloes. However, more research is warranted to determine the effect of EuO supplementation in production animals.

Effects of dietary enzyme cocktail on growth performance, intestinal morphology, and nutrient digestibility of weaned pigs

  • Kim, Yunkang;Baek, Jangryeol;Jang, Kibeom;Kim, Junsu;Kim, Sheena;Mun, Daye;Kim, Byeonghyeon;Kim, Younghwa;Park, Juncheol;Choe, Jeehwan;Song, Minho
    • Korean Journal of Agricultural Science
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    • v.44 no.4
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    • pp.513-518
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    • 2017
  • Soybean, one of most widely used swine feed component in the world, contains non-starch polysaccharides (NSP). The digestive system of weaned pigs is not yet fully developed, and thus weaned pigs cannot easily digest diets based on corn and soybean meal. Dietary exogenous enzymes supplementation has been intensively investigated to assist digestion of anti-nutritional factors, such as NSP. This experiment was conducted to investigate the effects of dietary enzyme cocktail on growth performance, intestinal morphology, and nutrient digestibility of weaned pigs. A total 36 weaned pigs ($5.92{\pm}0.48kg\;BW$; 28 d old) were randomly allotted to 2 dietary treatments (3 pigs/pen, 6 replicates/treatment) in a randomized complete block design. The dietary treatments were a typical diet based on corn and soybean meal (CON) and CON with 0.05% enzyme cocktail (Cocktail; mixture of xylanase, ${\alpha}-amylase$, protease, ${\beta}-glucanase$, and pectinase). Pigs were fed their respective diets for 6 wk. Growth performance, morphology of ileum, apparent ileal digestibility (AID) and apparent total tract digestibility (ATTD) of dry matter, crude protein, and energy of weaned pigs were measured. No significant differences (p > 0.05) were observed for growth performance for the duration of the experimental period, and morphology of ileum, and nutrient digestibility between CON and Cocktail treatment groups. Therefore, the results from the current study indicated that enzyme cocktail supplementation in diets had no influence on growth performance, intestinal morphology, and nutrient digestibility of weaned pigs.

Characteristics of Bentonite Filter Cake on Vertical Cutoff Walls Evaluated by Modified Fluid Loss Test (수정 fluid loss 시험을 이용한 연직 차수벽에 생성된 벤토나이트 필터케익 특성 평가)

  • Nguyen, The-Bao;Park, Moon-Seo;Lim, Jee-Hee;Choi, Hang-Seok;Han, Shin-In
    • Journal of the Korean Geotechnical Society
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    • v.27 no.2
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    • pp.53-62
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    • 2011
  • During the construction of vertical cutoff walls, filtration of bentonite slurry into the adjacent soil formation fabricates a layer of bentonite filter cake on the wall surface. The bentonite filter cake possesses much lower hydraulic conductivity compared to that of backfill materials in the cutoff wall. Hydraulic conductivity of bentonite filter cakes formed with three different types of bentonites has been measured by performing the modified fluid loss test under various pressure levels. Three different mixture ratios, 4, 6, and 8%, were selected for fabricating bentonite filter cakes to represent common field conditions. Two analysis methods for interpreting the experimental data from the modified fluid loss tests were employed to estimate hydraulic conductivity of the bentonite cakes. The range of hydraulic conductivities of the three bentonite cakes is between $2.15{\times}10^{-11}\;m/s$ and $2.88{\times}10^{-10}\;m/s$ which is 1 to 2.7 orders of magnitude lower than that of the design cutoff wall backfill. The stress distribution and thickness of the bentonite cakes were also evaluated in this paper.

Nutrient Digestibility, Ruminal Fermentation Activities, Serum Parameters and Milk Production and Composition of Lactating Goats Fed Diets Containing Rice Straw Treated with Pleurotus ostreatus

  • Kholif, A.E.;Khattab, H.M.;El-Shewy, A.A.;Salem, A.Z.M.;Kholif, A.M.;El-Sayed, M.M.;Gado, H.M.;Mariezcurrena, M.D.
    • Asian-Australasian Journal of Animal Sciences
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    • v.27 no.3
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    • pp.357-364
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    • 2014
  • The study evaluated replacement of Egyptian berseem clover (BC, Trifolium alexandrinum) with spent rice straw (SRS) of Pleurotus ostreatus basidiomycete in diets of lactating Baladi goats. Nine lactating homo-parity Baladi goats (average BW $23.8{\pm}0.4$ kg) at 7 d postpartum were used in a triplicate $3{\times}3$ Latin square design with 30 d experimental periods. Goats were fed a basal diet containing 0 (Control), 0.25 (SRS25) and 0.45 (SRS45) (w/w, DM basis) of SRS. The Control diet was berseem clover and concentrate mixture (1:1 DM basis). The SRS45 had lowered total feed intake and forages intake compared to Control. The SRS25 and SRS45 rations had the highest digestibilities of DM (p = 0.0241) and hemicellulose (p = 0.0021) compared to Control which had higher (p<0.01) digestibilities of OM (p = 0.0002) and CP (p = 0.0005) than SRS25 and SRS45. Ruminal pH and microbial protein synthesis were higher (p<0.0001) for SRS25 and SRS45 than Control, which also had the highest (p<0.0001) concentration of TVFA, total proteins, non-protein N, and ammonia-N. All values of serum constituents were within normal ranges. The Control ration had higher serum globulin (p = 0.0148), creatinine (p = 0.0150), glucose (p = 0.0002) and cholesterol (p = 0.0016). Both Control and SRS25 groups had the highest (p<0.05) milk (p = 0.0330) and energy corrected milk (p = 0.0290) yields. Fat content was higher (p = 0.0373) with SRS45 and SRS25 groups compared with Control. Replacement of BC with SRS in goat rations increased milk levels of conjugated linoleic acid and unsaturated fatty acids compared with Control. It was concluded that replacing 50% of Egyptian berseem clover with SRS in goat rations improved their productive performance without marked effects on metabolic indicators health.

Development of Herbal Chicken Porridge and the Establishment of Optimizing the Mixing Ratio (한방 닭죽 개발 및 최적 배합비율 확립)

  • Kim, Kyung-Yeon;Baik, Moo-Yeol;Park, Cheon-Seok;Choi, Sung-Won;Kim, Chang-Nam;Kim, Byung-Yong
    • The Korean Journal of Food And Nutrition
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    • v.26 no.1
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    • pp.22-28
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    • 2013
  • The objectives of this study were to develop porridge with medicinal herbs and chicken breast meat and to find out the optimum mixing ratio. Several herb extracts such as Acanthopanacis senticosus, Rehmannia glutinosa, Disocorea japonica, and Poria cocos Wolf were used in the ratio of 2, 1, 1, and 1, respectively. The optimum processing condition for herbal chicken porridge was determined by a design expert program. Seventeen experimental points were selected, and herb extracts (82~101 g), chicken breast meat (30~50 g), and glutinous rice (40~60 g) were chosen as the independent variables. The measured responses were preference of taste, DPPH radical scavenging, SOD-like activity, and cost. The optimum formulation of herbal chicken porridge using the numerical analysis was set at herbal extracts (101 g), chicken breast meat (38.66 g), and glutinous rice (41.34 g) with a 0.714 desirability value. DPPH radical scavenging effect, preference of taste and the cost showed a linear model, whereas SOD-like activity showed a quadratic model indicating a higher interaction among the mixture. As a result of proximate composition of optimized herbal chicken porridge, the contents of moisture, carbohydrate, crude protein, crude lipid, and ash contents were 76.4, 9.0, 1.4, 0.5, and 1.6%, the calories of the porridge was 46.1 kcal/100 g.

Studies of Solid-Liquid Phase Equilibria for Mixtures of N-vinyl-2-pyrrolidone+2-pyrrolidone and Growth Rate of N-vinyl-2-pyrrolidone Crystal Using Melt Crystallization (N-vinyl-2-pyrrolidone과 2-pyrrolidone 혼합물의 고-액 상평형 및 용융결정화를 이용한 N-vinyl-2-pyrrolidone의 결정성장속도 연구)

  • Kim, Sun Hyung;Seo, Myoung Do;Tak, Moon Seon;Kim, Woo Sik;Yang, Dae Ryuk;Kang, Jeong Won
    • Korean Chemical Engineering Research
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    • v.51 no.5
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    • pp.587-590
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    • 2013
  • Solid-liquid equilibria for mixtures composed of n-vinyl-2-pyrrolidone (NVP) and 2-pyrrolidone were measured as a basic study for the melt crystallization process to remove 2-pyrrolidone as impurity included in NVP. A differential scanning calorimeter (DSC) and a crystallizer were used and the experimental results obtained from two methods were similar. The mixture showed a eutectic system which has a single composition at the minimum melting temperature. Calculation results from simple thermodynamic equations were found in general agreements with present data. To determine the growth rate of NVP crystal which is important for the design of crystallization process, thicknesses of the crystal were measured with the time using a layer melt crystallizer. The growth rates increased as cooling temperatures decreased. Heat transfer coefficient correlated from present data was found to successfully describe the crystal growth behavior.

The Study on Speaker Change Verification Using SNR based weighted KL distance (SNR 기반 가중 KL 거리를 활용한 화자 변화 검증에 관한 연구)

  • Cho, Joon-Beom;Lee, Ji-eun;Lee, Kyong-Rok
    • Journal of Convergence for Information Technology
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    • v.7 no.6
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    • pp.159-166
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    • 2017
  • In this paper, we have experimented to improve the verification performance of speaker change detection on broadcast news. It is to enhance the input noisy speech and to apply the KL distance $D_s$ using the SNR-based weighting function $w_m$. The basic experimental system is the verification system of speaker change using GMM-UBM based KL distance D(Experiment 0). Experiment 1 applies the input noisy speech enhancement using MMSE Log-STSA. Experiment 2 applies the new KL distance $D_s$ to the system of Experiment 1. Experiments were conducted under the condition of 0% MDR in order to prevent missing information of speaker change. The FAR of Experiment 0 was 71.5%. The FAR of Experiment 1 was 67.3%, which was 4.2% higher than that of Experiment 0. The FAR of experiment 2 was 60.7%, which was 10.8% higher than that of experiment 0.

Effects of Eco-friendly Multi-enzyme on Growth Performance, Intestinal Morphology, and Nutrient Digestibility of weaned Pigs (친환경 복합효소제 첨가가 이유자돈의 성장, 장내 형태학, 영양소 소화율에 미치는 영향)

  • Kim, Seong-Ki;Cho, Myung-Woo;Kim, Jun-Su;Jang, Ki-Beom;Kim, Sheen-A;Mun, Da-Ye;Kim, Byeong-Hyeon;Kim, Young-Hwa;Park, Jun-Cheol;Choe, Jee-Hwan;Song, Min-Ho
    • Korean Journal of Organic Agriculture
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    • v.26 no.1
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    • pp.141-149
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    • 2018
  • This experiment was conducted to investigate the effects of multi-enzyme on growth performance, intestinal morphology, and nutrient digestibility of weaned pigs. A total 36 weaned pigs ($5.92{\pm}0.48kg\;BW$; 28 d old) were randomly allotted to 2 dietary treatments (3 pigs/pen, 6 replicates/treatment) in a randomized complete block design. The dietary treatments were a typical diet based on corn and soybean meal (CON) and CON with 0.1% multi-enzyme (Multi; mixture of ${\beta}-mannanase$, xylanase, ${\alpha}-amylase$, protease, ${\beta}-glucanase$, and pectinase). Pigs were fed their respective diets for 6 wk. Measurements were growth performance, morphology of ileum, apparent ileal digestibility and apparent total tract digestibility of dry matter, crude protein, and energy of weaned pigs. There were no significant differences on growth performance during overall experimental period. No differences were found for the morphology of ileum and nutrient digestibility between CON and Multi groups. Therefore, the results in the current study indicated that multi-enzyme supplementation in diets had no effects on growth performance, intestinal morphology, and nutrient digestibility of weaned pigs.

Optimization of Soy Sauce Production Conditions with Black Garlic Extract by Response Surface Methodology (반응표면 분석법에 의한 흑마늘 추출물이 첨가된 간장의 제조 조건 설정)

  • Shim, Hye-Jin;Kang, Min-Jung;Kim, Gyeong-Min;Lee, Chang-Kwon;Kim, Jeong-Hwan;Shin, Jung-Hye
    • Korean journal of food and cookery science
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    • v.32 no.3
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    • pp.307-315
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    • 2016
  • Purpose: The central composition design was used to optimize the mixture conditions of black garlic extract. Methods: The response surface methodology (RSM) was carried out from concentration of black garlic extract ($X_1$) and the amount of the black garlic extract ($X_2$) as independent variables, and salts ($Y_1$), reducing sugars ($Y_2$), the content of total phenolic compounds ($Y_3$) and ABTS radical scavenging activity ($Y_4$) as dependent variables. We confirmed the conditions that salinity was minimized and reducing sugar, total phenolic compounds and ABTS radical scavenging activity had maximum values through the response surface analysis. Results: All results had saddle points in originally set up conditions hence, ridge analysis was carried out for narrowing the experimental area. The minimum salt concentration was 16.03% at black garlic extract concentration of $14.84^{\circ}brix$ and contents of 9.26%. Reducing sugar content had maximum of value 7.30 g/mL at $24.58^{\circ}brix$ and contents of 8.08%. Total phenolic compounds contents and ABTS radical scavenging activity had maximum values at black garlic extract concentration of 20.33 and $25.02^{\circ}brix$. The results indicate that addition of black garlic extract contributed to increased reducing sugar, phenolic compounds contents and antioxidant activity of the soy sauce, but the salt concentration was not significantly affected. Conclusion: Based on the results of RSM, the optimum ranges of addition conditions for lowering the salt concentration and, increasing the sensory and functional ability of soy sauce were as follows: black garlic extract concentration of $15-25^{\circ}brix$ and content of 7.8-9.3%.

The effect of a soil amendment on phosphate efficiency in a low productive paddy soil (저위생산답(低位生産沓) 토양(土壤)에 대(對)한 개량제(改良劑)와 인산(燐酸)의 효과(效果))

  • Shim, Sang Chil;Song, Ki Joon;Kim, Chung Ja
    • Korean Journal of Soil Science and Fertilizer
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    • v.4 no.1
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    • pp.21-26
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    • 1971
  • Present work is concerned with the effects of a soil amendment (a mixture of basic slag with trace elements Cu, Mn, Zn and B) on the phosphate uptake by rice plants and in improving yield of rice in low productive paddy soils. The experiment was conducted at Kimpo-myun, Kimpo-kun, Kyunggi province which is characterized as "Akiochi" area and split plot experimental design was adapted. The results are summarized as follows; 1. Combined effect of the soil amendment and phosphate applications on the grain yield is pronounced, which is also characterized by the increased grain weight, maturing rate and seed setting rate. 2. Treatment of soil amendment appears to improve phosphate efficiency; grain weight, maturing rate and seed setting rate are all improved as the rate of phosphate application increased. 3. Phosphate tends to accelerate plant growth at earlier stages of plant development while the soil amendment retards the growth, inhibiting excessive tillering. 4. The soil amendment increases silicate and manganese, but decreases phosphate, copper and Zinc contents in the rice plants.

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