• Title/Summary/Keyword: mites

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Determination of amitraz by high-performance Liquid chromatography with photodiode array detection and method validation (HPLC-PDA를 이용한 Amitraz의 분석법 확립 및 검증)

  • Yun, Hyeong-Jun;Yun, So-Mi;Lee, Myoung-Heon;Son, Seong-Wan
    • Korean Journal of Veterinary Research
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    • v.48 no.1
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    • pp.33-38
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    • 2008
  • In veterinary medicine amitraz has been used as an insecticide to eliminates mites, lice, and ticks in dogs, cats, goats, swine and cattle. The objective of present study was to developed an analytical method using one-step extraction and determination of the amitraz in veterinary drugs by liquid chromatography (LC). The amitraz was analyzed by LC equipped with Waters XTerra RP18 ($4.8{\times}250mm;\;5{\mu}m;\;Waters,\;USA$) analytical column, using 75% acetonitrile (acetonitrile/D.W; 75/25) at 1.0 ml/ min. The UV-VIS detection of amitraz was made at 290 nm. Calibration graphs were linear with very good correlation coefficients ($r^2>0.9999$) from $80{\sim}120{\mu}g/ml$. The limit of detection was $0.09{\mu}g/ml $ and limit of quantification was $0.27{\mu}g/ml $. The method showed good intra-day precision (CV 0.05~0.09%) and inter-day precision (CV 0.06~0.18%).

A Case of Tsutsugamushi Disease (경북지방에서 발견된 Tsutsugamushi 병 치험 1례)

  • Park, Shie-Hwoa;Yoon, Sung-Chul;Lee, Young-Hyun;Chung, Jae-Chun;Kim, Chong-Suhi;Hong, Seok-Il
    • Journal of Yeungnam Medical Science
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    • v.4 no.1
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    • pp.173-176
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    • 1987
  • Tsutsugamushi disease is an acute, febrile, typhus-like disease of rural Asia transmitted by the bite of larval trombiculid mites. The disease is caused by infection with Rickettsia tsutsugamushi. Recently the authors experienced a case of tsutsugamushi disease which was serologically confirmed. Here we reports a case of tsutsugamushi disease which successfully managed with doxycycline and reviewed literatures on it briefly.

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Comparative Study on Life Style according to the Existence of Atopic Dermatitis in Middle School Student (중학생의 아토피피부염 유무에 따른 생활양식 비교 연구)

  • Lee, Hwa-Ja;Cho, Young-Ran;Park, Ji-Eun
    • Journal of the Korean Society of School Health
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    • v.18 no.2
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    • pp.93-104
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    • 2005
  • Purpose: This study compared lifestyles of middle school students with and without atopic dermatitis in order to obtain the baseline information for health education. Methods: This study involved 256 students from 20 middle schools in Busan who suffered from atopic dermatitis and 231 students from 2 middle schools in Busan who did not have atopic dermatitis. Results: Students with atopic dermatitis had a lower family income (탑=16.99, p<.001) and were more likely to come from a nuclear family (탑=10.28, p<.001) than control students. 98% of the students with atopic dermatitis had several skin symptoms. 78.5% of students with atopic dermatitis reported experiencing difficulties in their daily because of their condition. Students with atopic dermatitis took shorter baths and used soap less frequently than the students in the control group. The students with atopic dermatitis cleaned their houses and bedclothes more often than normal students in an attempt to remove cockroaches and house-dust mites. Students with atopic dermatitis also ate fast and instant foods more often than normal students. Conclusions: Some lifestyle differences exist between students with and without atopic dermatitis. Therefore, education and guidance may be important to promote good health and improve lifestyle quality.

Insecticidal Activity of Paecilomyces fumosoroseus SFP-198 as a Multi-Targeting Biological Control Agent against The Greenhouse Whitefly and The Two-Spotted Spider Mite

  • Kim, Jae-Su;Roh, Jong-Yul;Choi, Jae-Young;Shin, Sang-Chul;Jeon, Mun-Jang;Je, Yeon-Ho
    • International Journal of Industrial Entomology and Biomaterials
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    • v.17 no.2
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    • pp.181-187
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    • 2008
  • An entomopathogenic fungus, Paecilomyces fumosoroceus SFP-198 was isolated in Kyungbuk province, Korea and screened out for the control of Trialeurodes vaporariorum nymphs. It showed 72.5% efficacy against second instars of T. vaporariorum nymphs at 6 days after treatment in the laboratory. To select an active ingredient for the mass production and the formulation, SFP-198 culture products were compared based on their insecticidal activities against T. vaporariorum nymphs. Among them, conidia and blastospores showed much higher insecticidal activity than supernatants. Furthermore, SFP-198 conidia were more heat-resistant than blastospores, and also powder form of conidia was more stable than their suspension form. SFP-198 conidia showed high pathogenicity on not only T. vaporariorum but also Tetranychus urticae in the glasshouse. This result suggested that SFP-198 conidia can be used as a multi-targeting biological control agent against sucking agri- cultural pests, such as whiteflies and mites.

A Taxonomic Study of the Family Zerconidae(Acari.Mesostigmata) in the Korean Peninsula (한반도 구멍응애과 (진드기목, 중기문아목)의 분류학적 연구)

  • Lim, Jae-Won;Lee, Won-Koo
    • Animal Systematics, Evolution and Diversity
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    • v.17 no.2
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    • pp.191-205
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    • 2001
  • This study includes the taxonomy, description, and a key to species of mites in the Family Zerconidae(Acari, Mesostigmata) from the Korean Peninsula. Materials examined and information gathered were based on 1,020 specimens which has been collected nation-wide from April, 1994 to May, 2000. As a result of this study, the following 8 species were identified : Exhinozercon orientalis Blazak, 1975, Eurozercon pacificus Halaskova, 1979, Kaikiozercon mamillosus Halaksova, 1979, Kaikiozercon peregrinus Halaskova, 1979, Koreozercon bacatus Halaskova, 1979, Mesozercon coeranus Blaszak, 1975, Mesozercon plumatus (Aoki, 1966), Zercon szeptyckii Blaszak, 1976. Predominant species was Zercon szeptyckii which has been collected more then 600 specimens at 25 localities in the Korea Peninsula. Seven species except Mesozercon plumatus(Aoki, 1966) had been recorded from North Korea but newly recorded from the southern part of Korean Penunsula.

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A Study on the Domatia Structure of Broad-leaved Trees in Korea (한국산 활엽수종의 도마티아 구조에 대한 연구)

  • Kim, Gab-Tae;Choo, Gab-Cheul;Kim, Hoi-Jin
    • Korean Journal of Environment and Ecology
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    • v.24 no.3
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    • pp.258-263
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    • 2010
  • To study on the typical domatia structure, the leaves of six broad-leaved tree species were examined. Domatia of the leaves of the broad-leaved trees is the defense strategies against hervivory of many broad-leaved tree species. We observed four type domatia; Pouch type, Tuft type, Pocket type, and Pocket + tuft type. This result suggests that broad-leaved trees have develope leaf domatia structures for mutualism with predatory mites.

An Analysis on the Safety related to Architectural Elements in Housing (주거 공간 내 건축 구성 요소의 안전성 분석)

  • Yi, Hoon;Lee, Yong-Hee;Jeong, Sang-Kyu
    • KIEAE Journal
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    • v.5 no.4
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    • pp.9-16
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    • 2005
  • The purpose of this study is to analyze the safety issues with regard to architectural elements in housing. Causes of accidents and threats to health are reviewed for 'safety' aspects. Threats to health tend to draw more attention due to recent increase of SHS and its public perceptions. The majority of victims by accidents in residential houses are on juveniles aged under 14 in bathrooms and living rooms. Non-slip finish materials are recommended to floors for access and circulation and protective pads to furniture edges. Colored glass may increase the safety of the windows in the living room. Handles and non-slip mats will be a good addition for the safety. Human scale design of the kitchen system is mandatory and appropriate size of storages for hazardous equipment are to be provided. Passive air ventilation for better air quality by dwellers in residence is occasionally used whereas large-scale apartments provide mechanical ventilation to supplement the volume of fresh air. Since the internal air quality is proved one of the major causes of atopic and respiratory diseases, steady effort to achive better air quality utilizing appropriate materials, plants and equipment is mandatory. Frequent cleaning and the use of anti-fungus materials are necessary because House Dust Mites are believed to be one of main causes of such diseases.

Cloning and expression of trypsin-encoding cDNA from Blattella germanica and its possibility as an allergen

  • OCK Mee Sun;KIM Bong Jin;KIM Sun Mi;BYUN Kang Hyun
    • Parasites, Hosts and Diseases
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    • v.43 no.3 s.135
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    • pp.101-110
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    • 2005
  • In this study, the trypsin gene (bgtryp-1) from the German cockroach, Blattella germanica, was cloned via the immunoscreening of patients with allergies to cockroaches. Nucleotide sequence analysis predicted an 863 bp open reading frame which encodes for 257 amino acids. The deduced amino acid sequence exhibited $42-57\%$ homology with the serine protease from dust mites, and consisted of a conserved catalytic domain (GOSGGPLV). bgtryp-1 was determined by both Northern and Southern analysis to be a 0.9 kb, single-copy gene. SDS-PAGE and Western blotting analyses of the recombinant protein (Bgtryp-1) over-expressed in Escherichia coli revealed that the molecular mass of the expressed protein was 35 kDa, and the expressed protein was capable of reacting with the sera of cock-roach allergy patients. We also discussed the possibility that trypsin excreted by the digestive system of the German cockroach not only functions as an allergen, but also may perform a vital role in the activation of PAR-2.

The Effects of Bojungikgi-tang on the Nasal Tissue in Allergic Rhinitis of Ovalbumin0inhalation Rats. (보중익기탕이 알레르기 비염 유발 白鼠의 과립구 및 조직학적 변화에 미치는 효과)

  • Kim Jong-sung;Sim Sung-yong;Um Yu-sik;Nam Hae-jeong;Kim Kyung-jun
    • The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
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    • v.17 no.1
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    • pp.66-74
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    • 2004
  • Allergic rhinitis is an allergic reaction characterized by sneezing, coughing, itchy nose, mouth and throat, congestion and/or nasal discharge. The offending allergens are usually pollens, molds, dust mites and animal allergens. We aimed to observe inhibitory effects of Bojungikgi-tang on incerase of the number of eosinophil and neutrophil, and protective effects of nasal mucosal tissue in the allergic rhinitis. 1. In Inhibitory Effect of Bojungikgi-tang on Increase of the number of Eosinophil in Serum, Bojungikgi-tang showed significant effects.(p〈0.05) 2. In Inhibitory Effect of Bojungikgi-tang on Increase of the number of Neutrophil in Serum, Bojungikgi-tang showed significant effects.(p〈0.05) 3. In histopathologic change of nasal mucosal tissue, Bojungikgi-tang showed significant protective effects. Depending upon above results, it is considered that Bojungikgi-tang has the inhibitory effects on the process of allergic rhinitis and that it could be used in reliving patients of the symptoms caused by allergic rhinitis.

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New records to the Korean soil dwelling Mesostigmata fauna (Acari)

  • Kontschan, Jeno;Jeon, Mi Jeong;Hwang, Jeong Mi;Seo, Hong Yul
    • Journal of Species Research
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    • v.4 no.1
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    • pp.33-44
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    • 2015
  • A total of 34 species belonging to 11 families and 21 genera in the Order Mestostigmata are reported from the Korean Peninsula, of which 20/34 species are new for the fauna of the Korean Peninsula (Lasioseius furcisetus Athias-Henriot, 1959, Cheiroseius nepalensis Evans & Hyatt, 1960, Cheiroseius curtipes (Halbert, 1923), Hypoaspis imitatus Reitblat, 1963, Hypoaspis presternalis Willmann, 1949, Hypoaspis kargi Costa, 1968, Pseudoparasitus placentulus (Berlese, 1887), Gamasiphis novipulchellus Ma & Yin, 1998, Parholaspulus bregetovaae Alexandrov, 1965, Parholaspulus paradichaetes Petrova, 1967, Neparholaspis arcuatus Petrova, 1977, Neparholaspus simplex Evans, 1956, Parholaspulus excentricus Petrova, 1967, Gamasholapis asiaticus Petrova, 1967, Veigaia carpillaris Tseng, 1994, Veigaia anmashanensis Tseng, 1994, Leptogamasus bicorniger Witalinski, 1977, Neogamasus insignis (Holzman, 1969), Iphidinychus kakumeiensis Hiramatsu & Hirschmann, 1992, Trigonuropoda sanguinea Hirschmann & Hiramatsu, 1977). A total of 19 of the previously unreported species were collected in soil samples from the Democratic People's Republic of Korea (DPRK), while 13 species were collected from soil samples from the Republic of Korea (ROK). Only two species were found in both the DPRK and the ROK. Illustrations and short descriptions for previously unreported species provided.