• Asian Pacific Journal of Cancer Prevention
• /
• v.16 no.17
• /
• pp.7749-7754
• /
• 2015

Background: Currently, cationic liposome has become the commonly used vehicles for gene transfection. Furthermore, one of the most significant steps in microRNAs expression studies is transferring microRNAs into cell cultures successfully. In this study we aim to approach the feasibility of transfection of cervical cancer cell lines mediated by liposome and to obtain the optimized transfection condition for cervical cancer cell lines. Materials and Methods: $Lipofectamine^{TM}2000$ as the carrier, miR-101 mimic was transfected into Hela cells and Siha cells. Using green fluorescent protein as reporter gene, to set different groups according to cell seeding density, the amount of miRNA, miRNA and the proportion of Liposomes, Whether to add serum into medium to study their impact on the liposomal transfection efficiency. Finally, MTT assay was used to analyze the relative minimal cell toxicity of liposome reagents. Results: The seeding density of Hela cell line and Siha are $1.5{\times}10^4$ (per well of 24 well plates), miRNA amount is 1ul of both, the ratio of miRNA and liposome is 1:0.5 of Hela cell line; 1:0.7 of Siha cell line respectively, after 24 hours we can get the highest transfection efficiency. Compared with serum medium, only Siha cells cultured with serum-free medium obtained higher transfection efficiency before transfection (P<0.01). MTT assay showed that according to the above conditions which has the lowest cytotoxicity. Conclusions: The method of Liposome to transfected is a suitable way and it can be an efficient reagent for miRNA delivery for Hela cells and Siha cells in vitro. It may serve as a reference for the further research or application.

• Journal of the Institute of Electronics Engineers of Korea TC
• /
• v.44 no.10
• /
• pp.185-190
• /
• 2007

In this paper, minimum sampling rates and method of nonlinear characterization were suggested for low power, quasi-memoryless PAs. So far, the Nyquist rate of the input signal has been used for nonlinear PA modeling, and it is burdening Analog-to-digital converters for wideband signals. This paper shows that the input Nyquist rate sampling is not a necessary condition for successful modeling of quasi-memoryless PAs. Since this sampling requirement relives the bandwidth requirements for Analog-to-digital converters (ADCs) for feedback paths in digital pre-distortion systems, relatively low-cost ADcs can be used to identify nonlinear PAs for wideband signal transmission, even at severe aliasing conditions. Simulation results show that a generic memoryless nonlinear RF power amplifier with AMAM and AMPM distortion can be successfully identified at any sampling rates. Measurement results show the modeling error variation is less than 0.8dB over any sampling rates.

• Journal of Trauma and Injury
• /
• v.19 no.1
• /
• pp.59-66
• /
• 2006

Purpose: Acute liver failure after massive partial hepatectomy is critical condition with high mortality. To prevent postoperative liver failure from being induced by a massive partial hepatectomy, many doctors do a minimal resection on the single lobe of the liver that might cause postoperative bleeding from the remaining ruptured parenchyma. The objective of this study was to assess clinical experience with postoperative hepatic arterial embolization to control bleeding from the remaining ruptured liver during the postoperative period. Methods: This retrospective 4-year study was conducted from May 2002 to April 2006 and included consecutive patients who had sustained massive hepatic injuries and who had undergone a laparotomy, followed by postoperative hepatic arterial angiographic embolization to control bleeding. Data on the injury characteristics, the operative treatment and embolization, and the amount of transfused packed red cells (PRBC) were gathered and analyzed. In addition, data on the overall complications and survival rate were collected and analyzed. Results: Every case showed severe liver injury, higher liver injury scaling grade IV. Only ten cases involved a ruptured bilateral liver lobe. A lobectomy was done in 6 cases, a left lobectomy was done in 3 cases, and a primary suture closure of the liver was done in 2 cases. Suture closure was also done on the remaining ruptured liver parenchyma in cases of lobectomies. The postoperative hepatic arterial embolizations were done by using the super-selection technique. There were some cases of arterio-venous malformations and anomalous vessel branches. The average amount of transfused PRBC during 24 hours after embolization was $2.36{\pm}1.75$, which statistically significantly lower than that before embolization. Among the 11 cases, 9 patients survived, and 2 died. There was no specific complications induced by the embolization. Conclusion: In cases of postoperative bleeding in severe hepatic injury, if there is still a large amount of bleeding, postoperative hepatic arterial embolization might be a good therapeutic option.

• The Journal of Korean Academy of Prosthodontics
• /
• v.54 no.2
• /
• pp.132-139
• /
• 2016

The progressive attrition of teeth is a normal process by aging. However, excessive tooth wear with decreased vertical dimension of occlusion and collapse of occlusal plane may cause pathologic pulpal condition, occlusal disharmony, functional disorders and esthetic problems. The change of vertical dimension is required in case that there is no sufficient clearance for restoration or in case that the occlusal relationship is modified. For gaining the vertical dimension, a careful diagnosis is essential prior to starting the restoration treatment. After evaluating adaptation of neuromuscular system of patient during provisional phase, the final restorations can be fabricated. In this case, a 78 year old male with severely worn down dentition was treated. To improve the esthetic appearance and to achieve the ideal occlusal relationship, the full mouth rehabilitation with minimal increase of vertical dimension is planned and diagnostic wax-up was performed at the increased vertical dimension. After evaluation of provisional restorations for 12 weeks, final restorations were fabricated and routine clinical assessments were made. After 1 year, the restorations with newly established occlusal scheme are well maintained without significant complications and esthetically and functionally satisfactory results were obtained.

• Korean Journal of Cognitive Science
• /
• v.4 no.1
• /
• pp.177-212
• /
• 1993

My research is based on the parser for ill-formed input by Mellish in a paper in ACL 27th meeting Proceedings. 1989. My system is composed of two parsers:WFCP and IFCP. When WFCP fails to give the parse tree for the input sentence, the sentence is identified as ill-formed and is parsed by IFCP for error detection and recovery at the syntactic level. My system is indendent of grammatical rules. It does not take into account semantic ill-formedness. My system uses a grammar composed of 25 context-free rules. My system consistes of two major parsing strategies:top-down expection and bottem-up satisfaction. With top-down expectation. rules are retrieved under the inference condition and expaned by inactive arcs. When doing bottom-up parsing. my parser used two modes:Left-Right parsing and Right-to-Left parsing. My system repairs errors sucessfully when the input contains an omitted word or an unknown word substitued for a valid word. Left- corner and right-corner errors are more easily detected and repaired than ill-formed senteces where the error is in teh middle. The deviance note. with repair details, is kept in new inactive arcs which are generated by the error correction procedure. The implementation of my system is quite different from Mellish's. When rules are invoked. my system invokes all rules with minimal inference. My bottom up parsing strategy uses Left-to-Right mode and Right-to-Left mode. My system is bottom-up-parsing-oriented like the chart parser. Errors are repaired in two ways:using top-down hypothesis, and using Need-Chart which keeps the information of expectation and complection of expanded goals by rules. To reduce the number of top-down cycles. all rules are invoked simultaneously and this invocation information is kept in Need-Chart. This idea will be extended for the implementation of multiple error recovery system.

• The Korean Nurse
• /
• v.29 no.3
• /
• pp.25-35
• /
• 1990

It is a fact that there are recent increasing trends or incidence of the colorectal carcinoma among other colorectal diseases. In such trend, the early detection remain to be most important by the air contrast barium enema and total colonofiberscopy. There are several ways to prepare the colon for barium enema and colonofiberscopy which include several agents. The mechanical cleansing have been used most frequently since Brown's method was adapted to be most perfect for colon deansing. The ideal laxatives and enema solutions were limited to adequate dose, it's effectiveness, patient's diet and bowel habit, minimal side effect, low cost and simple to perform. In order to compare the effectiveness of various cleansing solutions, six experimental methods were formed as shown; 1. normal saline enema, 2. castor oil with normal saline enema, 3. castor oil with soap suds enema, 4. magnesium citrate with normal saline, 5. magnesium citrate with soap suds enema and 6. ingestion of Golyetly solution. The authors have compared and determined the degree of cleanness by an experienced endoscopist. The total number of patients was 247, age distribution was $43{\pm}15$ years old, and sex distribution was 133 males and 114 females. The grade I and II represented no difficulties at performing the colonofiberscopy, but grade ill and N had some difficulties, even unable to perform the colonofiberscopy. The effectiveness the cleansing agents, represented with grade I and II was 95.9% (47/49) in method 6, 93.2%(54/58) in method 2, 83.3%(30/33) in method 3, 70.0%(28/10) in method 5, 66.7%(16/24) in method i, and 45.7%(18/40) in method 4. Method 2 and 6 were the most effective in normal bowel habit patients. In constipated patients, method 6 was the most effective and all method except method 11 were effective in diarrhea patients. The degrees of less mucosal irritation by various bowel cleansing method were in the order of method 6(100%), 1(100%), 5(74%), 2(69%). In subjective symptoms and cleansing groups, abdominal distension, pain, nausea and vomiting were complained, and that's subject symptoms were in the order of method 3(88.9%), 6 (79.6%), 1(75%), 5(72.5%), 2(72.4%), 4(67.5%). In conclusion, we believe that the Golytely of the mechanical cleansing solution for colonofiberscopy was the most effective, but others depended on the patient's condition und bowel habit.

• Journal of Conservation Science
• /
• v.29 no.2
• /
• pp.161-170
• /
• 2013

The purpose of this study is to find the reasonable plan for the restoration method through the practical repair of excavated costume. The minimal repair only without adding the physical force as far as possible after investigating the state of excavated costume has been progressed in case of excavated costume since it is already degraded and weak due to the characteristics of organic matter at the moment when it is excavated. Moreover, it has been restored as the following course; The restoration has been applied according to the state of excavated costume with the maximum stability by classifying items capable of being restored considering the fabric state of excavated costume. The drawn thread from the weak in strength and the refined thread of the Juasa(simple gauze) or the light degraded silk thread has been used in case of thread for restoration. The cotton fabrics has been used in the fabric of cellulose system, the Juasa or the silk has been used in the silk depending on the kind of fabric in connection with the support fabric, some parts have been dyed with Alnus firma fruit and the persimmon, has been repaired by using the dyed fabric with the clove for the purpose of mothproof-antibacterial treatment. The method to repair has been carried out by classifying according to the state of damage and the way of formation of costume. The needlework method suitable for the part and state of damage by using the basting, the broad-stitching, backstitch, the blind stitch and the hemming stitch in connection with the needlework method for restoration.

• International Journal of Highway Engineering
• /
• v.5 no.2 s.16
• /
• pp.15-24
• /
• 2003

The performance of waterproofing system (WPS) is known to be a function of many complex interaction of material factors, design details, and the quality of construction, but it is mainly determined by the bond strength, which is measured by tensile adhesive strength (TAS) test. to the concrete bridge deck. In this research, eight waterproofing membranes were selected from commercial market and the tensile adhesive characteristics of the WPS on concrete bridge deck were investigated in view of various factor in asphalt pavement. The factors include type of asphalt mixture, pavement thickness, paving temperature and influence of wheel loading. TAS test of different asphalt pavement types showed that TAS of WPS under SMA (Stone Mastic Asphalt) pavement was greater than that under dense asphalt pavement. TAS of sheet membranes was improved as the compaction temperature of asphalt concrete increase, but TAS of liquid membranes were not. The influence of thickness of pavement val minimal with given laboratory test condition. TAS of sheet membranes after wheel tracking test were in the order of the sites under wheel path (UWP), before wheel tracking (BWT) and nearby wheel path (NWP). Since TAS of the same WPS of UWP was higher than TAS of BWT, wheel loading had function of pressing WPS resulting in higher adhesive strength. But liquid membranes were variable on types. The feature of detached interface after TAS test showed that sheet types were all detached in between deck concrete and WPS, and liquid types were detached in between asphalt pavement and WPS.

• Journal of Plant Biotechnology
• /
• v.32 no.3
• /
• pp.175-179
• /
• 2005

To develop a high efficiency plant regeneration system from in vitro cultures of strawberry, cv. Yeobong, petiole and leaf explants were cultured on MS basal medium containing a combination of 0.5 mg/L IBA and 3.2 mg/L kinetin or zeatin or benzyl amino purine (BAP) for 6 weeks, and leaf explants with dark pretreatment for a week ($T_1$), 2 weeks ($T_2$), and 4 weeks ($T_3$) were cultured on medium supplemented with 0.5 mg/L IBA and 3.2 mg/L zeatin under 16 hr photoperiod for 6 weeks. Shoot organogenesis was observed from the greenish calli containing minimal anthocyanin formed at proximal cutting edges of the leaf explant (57%) when cultured adaxial side on the medium, whereas was directly formed from a cutting edges of petiole explant (6.3%). Frequency of callus formation and shoot organogenesis at large size of leaf explant ($1.0{\sim}1.5\;cm^2$) was higher than small size ($0.5{\sim}1.0\;cm^2$), and dark pretreatment significantly improved the frequency of leaf explants that produced calli and shoots. The maximum frequency (87%) for shoot organogenesis was obtained from the leaf explants that transferred to a 16 hr photoperiod condition after the initial 4 weeks dark period. The improved frequency (87%) in comparision with control without dark pretreatment (27%). When the shoots were transferred to 1/2 MS basal medium, formed roots with 20 d of culture. The rooted plants were subsequently transferred to the pots and to the field.

• Journal of Hospice and Palliative Care
• /
• v.9 no.1
• /
• pp.35-39
• /
• 2006

A 77-year-old female presented panperitonitis due to advanced gastric ranter during palliative care. In the case of panperitonitis following obstruction or perforation, surgical treatment is vital to avoid fatal sepsis and dehydration. However, the risk of surgery and the residual life of a patient must be carefully considered because of high mortality and complication rate in those patients with advanced disease due to the poor condition. The therapeutic value of aggressive hydration, nasogastric tube insertion, and the use of antibiotics is also questionable. Palliative surgery was not performed on this case, and she passed away peacefully in the presence of family after 4 days of palliative medical care. Here is the appropriate management for this kind of patients we would like to recommend through review of relevant references and long discussions. Firstly, we need to predict survival time using clinical variables. Secondly, considering patient status and risk of surgery, non surgical palliative care such as pain control, transient nasogastric tube insertion, and parenteral hydration is recommended. Minimal use of fluid is desirable to minimize complications such as edema and dyspnea if massive hydration in the beginning of treatment is not proved to be effective. Even though started earlier in the course of disease, discontinuation of antibiotics could be discussed with patients and their caregiver if patient status is not improved.