• Journal of the Korean Society of Manufacturing Process Engineers
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• v.19 no.12
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• pp.42-48
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• 2020

Given the increasing interest in air pollution, several technologies to measure black carbon (BC) aerosol particles have been developed. As most BC aerosol particles are smaller than 1 ㎛, it is necessary to pre-separate the particles by size before a BC measuring instrument samples the aerosol particles. In this study, a miniature cyclone separator for portable BC measuring instruments was developed. A numerical approach was used to design the miniature cyclone separator with operating flow rates of 50, 100, or 150 mLPM, and then a prototype cyclone separator was manufactured for experimental validation. The numerical results of the cut-off size and pressure drop of the miniature cyclone separator agreed well with the experimental data. The cut-off sizes of the miniature cyclone separator were determined to be 2.9, 0.94, and 0.63 ㎛ for operating flow rates of 50, 100, and 150 mLPM, respectively. Thus, the miniature cyclone separator is suitable for use as a sampling inlet for the portable black carbon measuring instrument to sample BC aerosol or PM2.5 aerosol.

• Reproductive and Developmental Biology
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• v.35 no.3
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• pp.385-390
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• 2011

The purpose of this study was undertaken to evaluate of cryopreservation efficiency in ${\alpha}$ 1,3-galactosyltransferase knock-out(GalT KO) cloned miniature pig sperm. To compare ability of frozen-thawed sperm characteristics, three different pig strains (GalT KO) cloned miniature pig, PWG miniature pig and Duroc were used. The ejaculated semen from the three pig species was diluted with same volume extender and added to LEY solution for freezing. The diluted semen was placed in 0.5 ml straws, and freezing was initiated by exposing the straws to liquid nitrogen ($LN_2$) vapours for 10 min before placing them into $LN_2$ for cryopreservation. After thawing, the sperm ability were assessed for viability (SYBR-14/PI staining), abnormality (Rose Bengal staining), and acrosome status (intactness, intensity and capacitation) (chlorotetracycline, CTC staining). The viability of frozen-thawed GalT KO pig sperm had no significant difference as compared with Duroc and PWG miniature pig sperm. However, The CTC pattern of frozen-thawed GalT KO cloned miniature pig spermatozoa showed significantly lower rates in F pattern and AR pattern (p<0.05) and significantly higher rates in B pattern than Duroc and PWG miniature pig (p<0.05). The abnormality of GalT KO cloned miniature pig sperm was significantly lower as compared to Duroc and PWG miniature pig sperm (p<0.05). In conclusion, GalT KO cloned miniature pig semen can be cryopreserved successfully and used for artificial insemination reasonably.

• Journal of Veterinary Clinics
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• v.24 no.4
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• pp.593-596
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• 2007

The purpose of this study is to investigate normal hepatobiliary functions in healthy miniature pigs. $^{99m}Tc-DISIDA$ hepatobiliary scintigraphy(HBS) was used for it. Five mCi dose of $^{99m}Tc-DISIDA$ was injected intravenously into 3 healthy adult miniature pigs, and dynamic images were obtained during 1 hour. $^{99m}Tc-DISIDA$ HBS in a miniature pig was evaluated for 6 variables. A cardiac washout occurred within 1 min in all miniature pigs and radioactivities in the gallbladder were not detected in two miniature pigs. Thus, the initial radioactivity and Tmax of the gallbladder were non-available to identify. Mean Tmax of liver was $8.67{\pm}2.08$ min and initial small intestinal radioactivity was seen at $9.67{\pm}2.52$ min after $^{99m}Tc-DISIDA$ injection. Mean hepatic washout time was not detected in 60 min dynamic images. Therefore, $^{99m}Tc-DISIDA$ HBS is the effective diagnostic method to evaluate the function of hepatocyte and bile flow rate. However, it was not a proper method to evaluate the function of gallbladder, which indicates that an additional study is needed to further specify the reasons for the absence of radioactivities in gallbladder of two miniature pigs.

• Journal of Embryo Transfer
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• v.29 no.2
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• pp.119-125
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• 2014

Although the majority of surviving pigs cloned by somatic cell nuclear transfer (SCNT) appear to be physiologically normal, there is a general lack of detailed hemato-physiologic studies for the period of early adulthood to substantiate this claim. In the present study, we investigated variation in blood chemistry and endocrinological parameters between mesenchymal stem cells (MSCs) derived from cloned and normal age-matched female and male miniature pigs. Cloned females and males showed normal ranges for complete blood count assessments. Biochemical assessments showed that ${\gamma}$-GGT, ALT and cholesterol levels of male and female clones were significantly (P<0.05 or P<0.01, respectively) higher than that of age-matched control miniature pigs. Variations in insulin and IGF-1 were higher in female clones than in male clones and controls. Thus, although female and male cloned miniature pigs may be physiologically similar to normal animals, or at least within normal ranges, a greater degree of physiological and endocrinological variation was found in cloned pigs. The above variation must be taken into account before considering cloned female or male miniature pigs for various biomedical applications.

• Nuclear Engineering and Technology
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• v.48 no.3
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• pp.799-804
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• 2016

A miniature X-ray tube based on a carbon-nanotube electron emitter has been employed for the application to a dental radiography. The miniature X-ray tube has an outer diameter of 7 mm and a length of 47 mm. The miniature X-ray tube is operated in a negative high-voltage mode in which the X-ray target is electrically grounded. In addition, X-rays are generated only to the teeth directions using a collimator while X-rays generated to other directions are shielded. Hence, the X-ray tube can be safely inserted into a human mouth. Using the intra-oral X-ray tube, a dental radiography is demonstrated where the positions of an X-ray source and a sensor are reversed compared with a conventional dental radiography system. X-ray images of five neighboring teeth are obtained and, furthermore, both left and right molar images are achieved by a single X-ray shot of the miniature X-ray tube.

• Reproductive and Developmental Biology
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• v.33 no.4
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• pp.249-255
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• 2009

The miniature pig is considered to be a better organ donor breed for xenotransplantation than other pig breeds because the size of the organs of the miniature pig is similar to that of humans. In this study, we aimed at identifying differentially expressed genes in the miniature pig ovary during pregnancy. For this, we used the miniature pig ovary model, annealing control primer-based reverse transcription polymerase chain reaction (PCR), quantitative real-time PCR (qRT-PCR), and northern blotting analysis. We identified 13 genes showing differential expression on the based of pregnancy status and validated 8 genes using qRT-PCR. We also sequenced the full-length cDNA of ephrin receptor A4 (EphA4), which had a significant difference in expression level, and validated it by northern blotting. These genes may provide a better understanding of the cellular and molecular mechanisms during pregnancy in miniature pig ovary.

• Progress in Superconductivity and Cryogenics
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• v.9 no.1
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• pp.86-90
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• 2007

The cryoprobe used in cryosurgery should be fabricated in milimeter-order size for its practical usage. In general a miniature J-T(Joule-Thomson) refrigerator is applied to a cryoprobe. In case of the miniature J-T refrigerator, the mass flow rate of working fluid is small due to considerable friction in a minute flow path. For that reason, the miniature J-T refrigerator has a limited cooling power. To obtain the large cooling power from the J-T refrigerator, the refrigerator should have large mass flow rate and effective J-T temperature drop. These quantities are closely related to the geometry of the heat exchanger and the expansion nozzle in a cryoprobe, and are contradictory. The large mass flow rate leads to the small J-T temperature drop and vice versa in the miniature J-T refrigerator. Therefore, the optimal design of a cryoprobe to achieve maximum cooling power at fixed tube size and fixed operating temperature is required. This paper presents the design procedure of such case.

• Journal of the Korean Society for Precision Engineering
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• v.17 no.12
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• pp.16-19
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• 2000

• Journal of Life Science
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• v.15 no.1 s.68
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• pp.45-48
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• 2005

The aim of this study was to construct a correct pedigree of miniature horses (MH). The sex of MH was detected by PCR amplification of the sex determining region of the Y chromosome gene (SRY) prior to parentage testing. Ten random MH samples for parentage testing were genotyped by using 16 micro satellite markers. Since the SRY band (430 bp) was detected in horses No.1, 2, 6, 7, 8, 9, 10, these are male. However, the DNA segment was not identified in horses No.3, 4, and 5, which therefore are female. After genotyping, parentage testing was performed according to Mendelian fashion and International Society for Animal Genetics (ISAG) guideline. Of the 10 MH, 3 were qualified by the compatibility of 16 markers according to Mendelian fashion in the present DNA typing for parentage verification. These results can provide basic information for developing parentage verification and an individual identification system in MH.

• Proceedings of the Korean Nuclear Society Conference
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• 1996.05d
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• pp.182-187
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• 1996

Deformation characteristics of miniature plate tensile specimens have been studied to develop the thickness requirement and a correlation to estimate the mechanical properties of bulk material from miniature specimen data. The material used was a SA 508 C1.3 reactor pressure vessel steel and the thicknesses of miniature tensile specimens varied from ().12 m to 2 mm. The effects of thickness on the tensile deformation properties such as strength, ductility, and necking characteristics were analyzed. The yield and ultimate tensile strengths were independent of specimen thickness when the thickness was larger than about 0.2 mm. The uniform and total elongations decreased as the specimen thickness decreased. It was also observed that the uniform strain component in the width direction decreased with decrease in the specimen thickness, however, that in the thickness direction was rather constant in total thickness range studied. Based on this observation and a relationship between the necking angle and the ratio between strain components, a correlation between the uniform elongations of miniature specimen and standard specimen was derived. The uniform elongations calculated by this new correlation agreed well with the measured values.