• Journal of Animal Science and Technology
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• v.58 no.1
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• pp.2.1-2.10
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• 2016

Background: Heat stress adversely affects the physiological and metabolic status, and the productive performance of buffalo. Methods: The present study was conducted to explicate the effect of misting and wallowing cooling strategies during heat stress in lactating Murrah buffalo. The study was conducted for three months (May-July) of which first two months were hot dry and last month was hot humid. Eighteen lactating buffaloes, offered the same basal diet, were blocked by days in milk, milk yield and parity, and then randomly allocated to three treatments: negative control (no cooling), cooling by misting, and cooling by wallowing. Results: The results showed higher (P < 0.05) milk yield in buffaloes of misting and wallowing group compared to control during the experimental period however wallowing was found more (P < 0.05) effective during July (hot humid period). Both the treatments resulted into significant (P < 0.05) reduction in rectal temperature (RT) and respiratory rate (RR) compared to control animals during study period whereas wallowing was found to be effective on pulse rate (PR) only during July. Both treatments were resulted in mitigating the heat stress mediated decrease in packed cell volume (PCV), lymphocytopnoea and neutrophilia whereas decrease in total erythrocyte count (TEC) and monocytes was only mitigated by wallowing. Heat load induced alteration in serum creatinine and sodium concentration was significantly (P < 0.05) ameliorated by misting and wallowing whereas aspartate aminotransferase, alkaline phosphatase and superoxide dismutase activity, and reactive oxygen species concentration could be normalized neither by misting nor by wallowing. The significant (P < 0.05) increment in serum cortisol and prolactin levels observed in June and July period in control animals was significantly (P < 0.05) prevented by misting and wallowing. Conclusions: It can be concluded that misting and wallowing were equally effective in May and June (hot dry period) whereas wallowing was more effective during hot humid period in preventing a decline in milk production and maintaining physiological, metabolic, endocrine and redox homeostasis.

• Journal of the Korean Society of Food Science and Nutrition
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• v.14 no.4
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• pp.381-386
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• 1985

This experiment was conducted to investigate changes of soybean curd yield according to the extension of soaking time during manufacturing of soybean curd. To investigate those changes systematically, transmission electron microscopy and disc-gel electrophoresis were used. The soybean curd yield was increased from 45.0% to 50.5% and 55.4% respectively as soaking time is extended from 5 hours to 10 and 24 hours. The solid extraction and soybean milk coagulation were also increased according to the extension of soaking time. From disc-gel electrophoresis patterns of soybean milk protein and soybean curd protein, numbers of band were increased and major band thickened by expending the soaking time. Most of high molecular bands of soybean milk protein were transfered to soybean curd. Crude 7S proteins of soybean milk and soybean curd in dis-gel electrophoresis were appeared to be 4 and 5 bands respectively, and crude 11S proteins of soybean milk and soybean curd were appeared to be 9 and 8 bands respectively. Of soybean milk bands, most of 11S component transfered to soybean curd. Transmission electron photomicrographs revealed that the dimension of each protein body became larger and the numbers of spherosome around the protein bodies in unit area fewer by extending the soaking time of soybean.

• Asian-Australasian Journal of Animal Sciences
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• v.31 no.8
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• pp.1259-1266
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• 2018

Objective: This study was carried out to investigate the possible application of Broussonetia papyrifera (B. papyrifera) silage as a functional feeding stuff in dairy cattle. Methods: Seventy-two Holstein cows were divided into four groups randomly and allocated to 6 pens with 3 individuals in each group and fed the original total mixed ratio (TMR) in the dairy farm or the new TMR with 5%, 10%, and 15% B. papyrifera silage, separately. Feed intake were recorded, milk and blood samples were collected, and milk composition, blood metabolites and milk fatty acids composition were measure at the end of the experiment. Results: Dry matter intake of cows decreased when they fed on diet with B. papyrifera, but no differences were observed in body condition score, milk yield, milk protein and lactose, feed efficiency and serum metabolites between groups. Both 10% or 15% of B. papyrifera silage in the diet significantly increased the immunoglobulin A (IgA) and IgG in serum, 15% of B. papyrifera silage increased the content of serum catalase, superoxide dismutase, total antioxidant capacity, and decreased the content of 8-hydroxy-2'-deoxyguanosine. Furthermore, 10% or 15% of B. papyrifera silage resulted in a significant decrease in the milk somatic cell count, and increased the polyunsaturated fatty acids content in the milk. Conclusion: The diets with 10% to 15% of B. papyrifera silage might enhance the immune and antioxidant function of dairy cows and increase the polyunstaturated fatty acid concentration in the milk.

• Asian-Australasian Journal of Animal Sciences
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• v.14 no.5
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• pp.646-650
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• 2001

The effect of feeding concentrate mixture devoid of grain on the performance of crossbred dairy cows was studied. Twelve crossbred cows of first/second lactation were randomly distributed into two equal groups. The animals of group 1 were fed on a concentrate mixture containing 30% maize grain whereas, the cows of group 2 were offered a concentrate mixture where grain was completely replaced with deoiled rice bran (DORB) and molasses. Wheat straw was given ad libitum to the cows of both the groups. The feeding was continued for 112 days. The intake of dry matter, CP and TDN were similar in both the groups. Digestibility of DM, OM, EE, NDF and ADF were also comparable between the groups. The average daily fat corrected milk (FCM) yield was 7.70 kg and 7.43 kg in group 1 and 2, respectively. The chemical composition of milk (protein, fat and total solids) also remained unaffected. The animals of both the groups gained 9-10 kg body weight which indicates that both the diets were nutritionally adequate and grain can be successfully replaced with DORB and molasses from the diet to sustain about 6-7 kg FCM production.

• Asian-Australasian Journal of Animal Sciences
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• v.24 no.10
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• pp.1386-1392
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• 2011

The aim of the present work was to study the effects of a polyherbal supplement on cross bred does, starting from the last month of pregnancy to weaning, on milk yield, kid birth weight and growth rate. Thirty does were divided into three treatments of ten each in individual pens: low level supplementation (LS), high level supplementation (HS) and non-supplemented treatment (NS) as control. Low supplemented goats were given 125 mg/kg BW/d of polyherbal combination; high supplemented goats were given 250 mg/kg BW/d. The study was carried out in 2008. Fifty-nine kids were born from all the experimental animals. There was no difference on milk yield between supplemented groups and control (p>0.05), although polyherbal supplementation had positive effect on litter birth weight and growth rate compared to control. Weaning weights were higher (p<0.001) in LS and HS compared to NS does. In both supplemented treatments compared to control, mortalities and morbidities were also lower in kids born. It is concluded that pre-partum to weaning supplementation increases kids growth rates and weaning weights, as well as reduces kid mortalities, but it doesn't have significant effect on milk production.

• Journal of Animal Environmental Science
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• v.5 no.3
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• pp.181-188
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• 1999

This study was carried out to investigate the effect of blowing fan for reducing heat stress in lactating dairy cows in summer. Treatment conditions were divided into 3 types ; No blow(T1), blow to oblique angle(T2) and blow to right angle(T3). Feed intake milk yield, respiration rate, rectal temperature and plasma cortisol concenration were measured to investigate the effect of hot environment on the physiological, productive responses in lactating dairy cows. The results obtained are summarized as follows: The daily milk yields were 20.47, 22.82 and 24.31kg per head in T1, T2 and T3 during experimental period, respectively. The respiration rate was significantly (p<0.05) higher in T1 than those of T2 and T3. The rectal temperature in T1(39.4$^{\circ}C$) was higher than those of T2(39.1$^{\circ}C$), (P<0.05). The plasma cortisol concentration was higher in T1 than those of T2 and T3(P<0.05). It was suggested that blowing fan installed in the pen was effective means to reduce heat stress.

• Journal of Animal Science and Technology
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• v.63 no.2
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• pp.405-416
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• 2021

The aim of the study was to determine the effect of whole milk powder (WMP) as heterologous proteins on chicken breast emulsion-type sausages. The quality properties of WMP on such chicken breast emulsion-type sausages were investigated by measuring the proximate composition, pH, color, cooking yield, protein solubility, and by applying other methods, such as texture profile analysis (TPA), microphotograph, sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), and electronic nose. The crude fat, protein, and ash contents of 15% WMP samples were significantly higher than the control samples (p < 0.05). The redness of the cooked samples significantly increased with an increase in the WMP contents (p < 0.05). The cooking yield of WMP treated samples was significantly higher than the control sample (p < 0.05). Additionally, the hardness, gumminess, and chewiness of WMP treated samples were significantly higher than the control sample (p < 0.05). The sarcoplasmic and myofibrillar proteins of samples containing 15% WMP were significantly higher than the control samples (p < 0.05). The result of SDS-PAGE showed that the C protein, sarcoplasmic protein, actin, and tropomyosin increased with an increase in the WMP contents. The principal component analysis plot of WMP-treated samples was clearly different from that of the control samples. Based on these results, it was predicted that WMP could be useful as heterologous protein on emulsion-type sausage.

• Asian-Australasian Journal of Animal Sciences
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• v.29 no.12
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• pp.1734-1741
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• 2016

This study was conducted to examine the effects of adding phytoncide oil extracted from Korean pine nut cone by-product to the diet of dairy cows on milk yield and compositions, fatty acid characteristics, complete blood count and stress response. A total of 74 Holstein cows were used for 30 days and divided into two groups. Each group was given a basal diet (C) or an experimental diet containing phytoncide additives at 0.016% (T) in feed. The results showed that phytoncide feeding had no effect on milk yield. In addition, there were no observed effects on milk composition, but the ratio of fatty acid in milk was significantly affected by the phytoncide diet, and it showed a positive effect. Not only were the major functional fatty acids, conjugated linoleic acid and eicosapentaenoic acid increased, but also ${\omega}6:{\omega}3$ fatty acid ratio was reduced in milk of T group (p<0.05). In blood analysis, the complete blood count showed no significant difference between C and T group on all parameters. However, the cortisol concentration was significantly decreased in T group compared to control (p<0.05). Taken together, we suggest that phytoncide oil does not have a great influence on the physiological changes, but can be a potential feed additive that improves the milk fatty acid and stress resilience in dairy cows. In addition, it will contribute to the development of feed resource, a reduction in feed cost and a lessening of environmental pollution.

• Food Science of Animal Resources
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• v.33 no.4
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• pp.487-492
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• 2013

The objective of this study was to examine the effect of supplementation of high linoleic ($C_{18:2}$) oil or high linolenic ($C_{18:3}$) oil to the diet on milking performance and content of conjugated linoleic acid (CLA) isomers in goat milk fat. Forty five dairy goats (Sumnen, 25 d post-partum, $62.5{\pm}1.2kg$) were randomly assigned to three treatment groups with each group of 15 dairy goats. The goats were fed a basal diet (CON) consisting 1.2 kg concentrate and 1.2 kg chopped hay (0.6 chopped alfalfa and 0.6 kg hay) daily with 4% soybean oil (SO) or 4% linseed oil (LO). Daily feed intake was not influenced (p>0.05) but daily milk yield (p<0.001) and milk fat yield (p<0.001) were significantly increased by supplementation of oils. Supplementation of oils decreased the short chain fatty acid, medium-chain fatty acid and saturated fatty acid in goat milk fat while increased trans vaccenic acid (trans-11-$C_{18:1}$, TVA), oleic acid ($C_{18:1}$), $C_{18:2}$, $C_{18:3}$, cis-9, trans-11-CLA (c9, t11-CLA), trans-10, cis- 12-conjugated linoleic acid (t10, c12-CLA), unsaturated fatty acids, mono unsaturated fatty acid and long-chain fatty acid in goat milk fat (p<0.001). Especially, c9, t11-CLA, t10, c12-CLA and ${\omega}-3$ fatty acid ($C_{18:3\;n-3}$) in milk fat were highest when goat fed LO diet. Based on the result, it is suggested that supplementation of linseed oil should be an effective method to increase CLA isomers and ${\omega}-3$ fatty acid in goat milk fat without negative effect on lactating performance.

• Korean Journal of Food Science and Technology
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• v.30 no.3
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• pp.574-580
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• 1998

The technical feasibility of removing cholesterol from milk fat by supercritical carbon dioxide $(SC-CO_2)$ extraction followed by adsorption on different adsorbents and of fractionating milk fat into different fatty acid composition at $40^{\circ}C/276$ bar was investigated. Cholesterol could be selectively removed from milk fat by adsorption on a typical commercial florisil with $SC-CO_2$ extraction. Lower weight ratio of milk fat feed to florisil showed higher reduction of cholesterol, but gave lower yield in the milk fat fractions. The effective capacity of florisil for removing cholesterol from milk fat was 2.0g/g, which is the ratio of the fat feed to the adsorbent for 89% cholesterol reduction with a fat yield of 57.5%. Fatty acid composition showed higher short-chain and lower unsaturated long-chain fatty acids in the extracted fractions. Milk fat fractionation method by supercritical fluid extraction in coupled with adsorption would appear suitable for removing undesirable ingredients such as cholesterol and for enriching short-chain fatty acids in the fractions.