Major objectives were to evaluate effects of three schemes of bST-supplementation of Holstein cows (142.8 mg/14 d, POSILAC) during the prepartum and/or postpartum periods through 63 d (${\pm}3d$) of lactation. Measures evaluated the potential of treatments to improve body weight (BW) and body condition score (BCS), provoke changes in plasma concentrations of somatotropin (ST) and IGF-I, and improve milk yield, milk composition (percentages of protein and fat, and somatic cell counts), and several calving variables. Multiparous Holstein cows were randomly assigned to a $2{\times}2$ factorial arrangement of treatments (TRT) to give four groups (I = no bST, n = 26; II = bST postpartum, n = 25; III = bST prepartum, n = 27; IV = bST prepartum and postpartum, n = 25). During the prepartum period, cows in groups I and II were not supplemented but those in groups III and IV were supplemented every 2-wk beginning 21 d before expected calving date through calving. During the first 63 DIM only cows in groups II and IV were supplemented with bST. From 64 DIM through the end of lactation cows in all groups were supplemented with the full lactation dose of bST (500 mg/14 d). The BW and BCS were recorded weekly throughout the prepartum and postpartum periods and every 2-wk beyond 70 DIM. Blood samples were collected 3-times a week for analyses of ST and IGF-I. Milk yields were recorded daily though 150 DIM. Prepartum supplementation of bST did not affect BW or BCS, but mean concentrations of ST were increased 12.2% and were 15.5% greater at calving. Overall, mean concentration of IGF-I was not affected by treatment but concentrations were greater at 1 and 2 wk before calving in bST-supplemented cows. During the first 63 DIM the BW and BCS were not affected by treatment. Significant effects of bST-supplementation were detected on concentrations of ST, IGF-I and on milk yield compared to non-supplemented cows in group I. Postpartum concentrations of ST were greater in bST-supplemented cows (TRT II and IV; +41.9 and 54.6%). However, concentrations of IGF-I were greater only in cows in group IV (+25.9%) during the postpartum period. Overall, the three bST-supplemented groups had greater actual milk yield than the control group (I) during the first 63 and 150 DIM. The actual milk yields during 63 and 150 DIM were 6.5 and 4.6 kg/d greater for cows in group IV than cows in group I and the 305-d ME milk yield also was 15.6% greater. No adverse effects of TRT were observed on calf birth weight, colostrum immunoglobulins, ease of calving or other measures evaluated.
Two experiments were carried out to investigate the effect of Murrah buffalo cows pulling sledges under field conditions on milk production and milk composition. In Exp. 1, 24 buffaloes in the fourth month of lactation were used. They were allotted to four treatments according to a $2{\times}2$ factorial arrangement: work or no work, and with or without urea-molasses cake supplementation (700 g/animal/day). Feeds consisted of 20 kg fresh elephant grass (18% DM), 2 kg rice bran per day and rice straw ad lib. The animals worked in pairs three hours per day (work done: $3464{\pm}786kJ/d$) five days a week for three months. Three teams worked in the morning and the others worked in the afternoon in the same day. The following day the working times were switched. In Exp. 2, 16 lactating Murrah buffalo cows in the sixth month of lactation were allotted to two groups (work and no work). They were fed with fresh ruzi grass (Brachiaria ruziziensis) ad lib. supplemented with 2 kg rice bran and 700 g urea-molasses cake. The working regime was similar to that of the first experiment (work done: $3753{\pm}879kJ/d$) and they worked for two months. In the first experiment, there was a small but significant drop (p<0.05) in milk yield from 3.5 to 3.0 kg/day due to work, but there was no supplementation effect. The working buffaloes lost 5.2 kg whereas the non-working animals gained 9.7 kg during the three months (p<0.05). Supplementation increased live weight by 9.9 kg as compared to -5.4 kg for those not supplemented (p<0.05). Milk composition was not affected by the treatments. In the second experiment, daily milk production was similar for both treatments and approximately 3 kg. No significant differences were found in milk composition or in live weight changes for working and non-working groups, respectively. It was concluded that work may cause a reduction in milk yield and a loss of live weight on a poor rice straw diet but that an appropriate supplementation can alleviate this situation.
Automatic milking systems (AMS) rely upon voluntary cow traffic (the voluntary movement of cattle around a farm) for milk harvesting and feed consumption. Previous research on conventional milking systems has shown differences between dairy cow breeds for intake and milk production, however, the ability to manipulate voluntary cow traffic and milking frequency on AMS farms through breed selection is unknown. This study investigated the effect of breed (Holstein Friesian versus Illawarra) on voluntary cow traffic as determined by gate passes at the Camden AMS research farm dairy facility. Daily data on days in milk, milk yield, gate passes and milking frequency for 158 Holstein Friesian cows and 24 Illawarra cows were collated by month for the 2007 and 2008 years. Illawarra cows had 9% more gate passes/day than Holstein cows over the duration of the study; however, the milking frequency and milk yield of both breeds were similar. Gate passes were greatest for both breeds in early lactation and in the winter (June to August) and summer (December to February) seasons. These findings highlight an opportunity to translate increased voluntary cow movement associated with breed selection into increased milking frequencies, milk production and overall pasture-based AMS performance.
This study was conducted to investigate the relationship between lactational capacity and intakes of energy and energy-yielding nutrients. Food consumption, intakes of carbohydrate, protein, fat and energy and quantity and proximate composition of milk of 11 lactating Korean mothers were determined at 1, 2 and 3 months postpartum longitudinally. Food consumption was estimated using a 24-hour recall method ; intakes of energy and energy-yielding nutrients were calculated according to the Food Composition Table. Daily milk production was estimated using a 72-hour test-weighing method ; protein, fat, lactose and energy concentrations were analyzed. Average intakes of energy and protein were 1974$\pm$386㎉/day and 67.0$\pm$12.3㎉/day, these were 73% and 74% of the Korean Recommended Allowances, respectively. Average milk yield was 720.1$\pm$123.3ml/day containing energy 59.6$\pm$9.5㎉/dl, protein 1.1$\pm$0.1g/dl, fat 2.7$\pm$0.8g/dl, and lactose 6.3$\pm$0.3g/dl. No relationship existed between the intakes of carbohydrate, protein, fat and energy and the quantity and proximate composition of the milk. However, the intakes of energy, carbohydrate and vegetable protein were inversely related to the concentrations of energy and lactose in the milk. This result indicates that lactational capacity may be affected by the other factors excluding intakes of energy and energy-yielding nutrients and a high intake of energy may not guarantee optimal lactational capacity.
To determine the effects of dry period (DP) length on milk yield, milk composition, some blood metabolites, complete blood count (CBC), body weight and score and follicular status, twenty five primiparous and multiparous Holstein cows were assigned to a completely randomized design with DP-60 (n = 13) and DP-20 (n = 12) dry period lengths. Cows in the DP-60 produced more milk, protein, SNF, serum non-esterified fatty acids (NEFA) and beta hydroxyl butyrate acid (BHBA) compared with cows in DP-20 ($p{\leq}0.05$). Serum glucose, blood urea nitrogen (BUN), urea, and glutamic oxaloacetic transaminase (GOT) were all similar among the treatments. Body Condition Score (BCS), body weight (BW), complete blood count (CBC) and health problems were similar between the treatments. Diameter of the first dominant follicle and diameter of the dominant follicle on d 14 were different among the treatments. Thus, results of this study showed that reducing the dry period length to DP-20 had a negative effect on milk production, milk composition and reproductive performance in Holstein dairy cows.
Data were compiled and statistically analyzed on the lactation of 50% and 75% Thai native-Friesian crossbred and purebred Friesian cows that were fed at a national institute in Chiang Mai, Thailand. More than 30% higher milk production was obtained in the 75% crossbred compared with that in the 50%, but this amount of milk production in the upgraded breed was still about half that of purebred Friesians; 2,138 kg, least squares means during an average lactation period of 279 days in the 50% crossbred, 2,847 kg during 277 days in the 75% crossbred and 5,585 kg during 308 days in the purebred. Environmental stress due to tropical climate was alleviated by the use of electric fans and water sprinklers in the feeding house during the hot season, and improved diet seemed to enable purebred Friesians to keep their ability to produce a milk quantity of more than 6,500 kg per year. This special care was not given to crossbreds and significantly negative correlations were found between daily minimum temperature and humidity during the initial 100 days of lactation and total milk production and average daily milk yield in the 75% crossbreds. However, these correlations were not found in the 50% crossbreds.
Membrane technology has revolutionized the dairy sector. Different types of membranes are used in the industry for various purposes like extending the shelf life of milk without exposure to heat treatment, standardization of the major components of milk for tailoring new products as well increasing yield and quality of the dairy products, and concentrating, fractionation and purification of milk components especially valuable milk proteins in their natural state. In the cheese industry, membranes increase the yield and quality of cheese and control the whey volume, by concentrating the cheese milk. With the advancement of newer technology in membrane processes, it is possible to recover growth factor from whey. With the introduction of superior quality membranes as well as newer technology, the major limitation of membranes, fouling or blockage has been overcome to a greater extent.
This study was carried out to investigate the addition of Pueraria derived isoflavone into milk by means of microencapsulation technique. The yield microencapsulation sensory attributes, and capsule stability of Pueraria derived isoflavone microcapsules in milk were measured during 12 days. Coating materials used was polyglycerol monostearate(PGMS. The encapsulation yield of Pueraria derived isoflavone was 72.5% with PGMS when the ratio of coating material to core material was 15:1. The rate of pueraria derived isoflavone release was 15, 20, and 25% when stored at 4, 20, and $30^{circ}C$ for 12 days in milk respectively. In sensory evaluation, beany flavor and color of microcapsuled Pueraria derived isoflavone added milk were significantly different from control and uncapsuled Pueraria derived isoflavone added milk, however, bitterness was not significantly different. In vitro study, microcapsules of pueraria derived isoflavone in simulated gastric fluid with the range of 3 to 6 pHs were released 3.0${\sim}$15.0%, however, the capsules in simulated intestinal fluid with pH 7 were released 95.7% for 40 min incubation time. In conclusion, this study provided that PGMS as coating materials was suitable for the microencapsulation of Pueraria derived isoflavone, and the capsule containing milk was not affected with sensory attribute.
The objective of this work was to establish an approaching method for TMR feeding system in the farm situation by testing the effects of herd-mix feeding system on the performance of lactating cows. Fifty six Holstein cows were fed for the experimental period of 16 months. Prior to test the herd-mix feeding system, animals were kept on conventional feeding system for 4 months, separate feeding of forage with concentrate, then provided 3 types of herd-mix rations formulated by mean level of energy concentration requirement of higher 1/2 ranker in each herd for remaining 12 months to compare the effect on milk yield performance of animals with conventional feeding system by using a switch-over method. The herd-mix feeding system influenced substantially upon the improvements of milk yield(P<0.01) and milk fat percentage(P<0.05). In all of lactational ages, milk yield and milk fat were enhanced by the herd-mix feeding system. Especially, actual milk yield(AMY) and milk fat in the 1st lactating cows, and AMY and 4.0% fat corrected milk yield(FCM) in the 2nd lactating cows were increased signiticantly by the herd-mix feeding system(P<0.05). In the early and mid-stage of lactation, the herd-mix feeding system showed higher AMY and FCM compared with the conventional feeding system although the milk fat was not different. AMY, milk fat and FCM in the late-stage of lactation were increased generally by the herd-mix feeding system. Especially, milk fat and FCM obtained by the herd-mix feeding system were significantly higher than those by the conventional feeding system(P<0.01). The herd-mix feeding system showed a good type of milking curve with a higher persistency of FCM(93.24%) than in conventional one(92.69%). The income over feed cost with the herd-mix feeding system was lA-fold higher than that with the conventional feeding method. In conclusion, the results of this work suggest that the herd-mix feeding system based on a correct level of energy concentration of TMR and well-determined feed ingredients increases not only milk yield of dairy herd by enhancing the performance of cows in early to mid stage of lactation with improved milk persistency but also gross income of dairy farm.
This study was conducted to estimate the effects of year, age of dam at calving, farm and lactation period on milk yield with the data of 4,008 cows' records which were collected at 32 farms by Korea Animal Improvement Association from 1985 to 1989. The results obtained in this study are summarized as follows: 1. The average performance of the dairy cattle in the study were $5,959.23{\pm}2,113.03kg$ in actual milk yield, $49.19{\pm}22.77$ months in age of dam at calving, $27.11{\pm}5.13$ months in age at first calving and $255.11{\pm}79.68$ days in lactation period. 2. The percentages of variance component for different sources were 29.39% for the residuals, 1.91% for years, 4.86% for age at calving, 8.89% for farms and 54.94% for lactation period. 3. The overall mean of least-square estimate on the milk yield was 6,229.31kg. In the effects of year, the least-square means of milk yield were estimated 6,000.76kg in 1985-1987, 6,028.11kg in 1988 and 6,659.07kg in 1989. 4. The least-square means of calving age on the milk yield were estimated 5,456.01kg in less than 24 months, 6,565.48kg in 61-66 months which were the highest least-square means. This effects were gradually increased until the 61-66months and gradually decreased after the 61-66months, with highly significant differences among different months of age at calving(p<0.01). 5. In the effects of farm, the least-square means of milk yield were estimated 4,959.50 kg in the lowest farm and 7,497.07kg in the highest farm. Among the milk yield of each farm the effects showed highly significant difference(p<0.01). 6. The least-square means of milk yield in the effects of lactation period were gradually increased with the lapse of the lactation period. Among the lactation period the effects showed highly significant difference(p<0.01).
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