• Animal Bioscience
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• 제35권11호
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• pp.1725-1732
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• 2022

Objective: This study was conducted to investigate the effects of selenium benefits on the physiological responses, litter performance, blood profiles and milk composition of lactating sows and tissue concentration of their progeny when mixed form of selenium was provided in a lactation diet. Methods: A total of 45 multiparous sows (Yorkshire×Landrace) with similar body weight, backfat thickness, and parity were assigned to one of three treatments with 15 sows per treatment in a completely randomized design. Organic and inorganic selenium were mixed and added to the diet at 0.15 ppm and 0.25 ppm, respectively. A non-Se-fortified corn-soybean meal basal diet served as a negative control. Treatments were as follows: i) Control: corn-soybean meal based diet, ii) ISOS15: control+ inorganic Se 0.15 ppm+organic Se 0.15 ppm, iii) ISOS25: control+inorganic Se 0.25 ppm+organic Se 0.25 ppm. Results: Serum selenium concentrations of sows and piglets were increased by the supplemental Se mixture at 7 days of lactation compared with the control (p<0.01, respectively). The kidney and muscle selenium concentrations of piglets were increased by the supplemental Se mixture at 21 days of lactation compared with the control (p = 0.03; p = 0.04, respectively). Conclusion: Consequently, supplementation with mixed inorganic and organic selenium in a lactating diet could improve the selenium status of sows and piglets; no differences were observed among the mixing levels.

• 한국동물위생학회지
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• 제46권1호
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• pp.1-13
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• 2023

Fatty liver hemorrhagic syndrome (FLHS) is a metabolic disorder found in caged layer hens and causes reduced egg production and sudden death. Dandelion (Taraxacum coreanum, TC) and milk thistle (Cirsium japonicum var. ussuriense, CJ) are well known wild herbs inhabiting Korean peninsula and presenting antioxidative effects. This study investigated alleviate effects of these herbal mixture (6:4, w/w) composed of dried powder of TC and CJ against fatty liver in laying hens. The herbs mixture 5.0, 10.0, 20.0 or 40.0 g/kg feed was provided via feed admixture for 3 weeks to laying hens having FLHS. FLHS was induced by intramuscular injection of β-estradiol (2 mg/kg bw) 2 times per week for 3 weeks and supply with high caloric feed. Egg production rate was reduced from 76.2% at pre-treatment to 49.4% at 1 week and further decreased according to β-estradiol treatment. Increment of serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), total cholesterol (T-Chol) and total bilirubin (T-Bil) and decrement of serum superoxide dismutase (SOD) and glutathione peroxidase (GPX) with fatty liver were found by the treatment of β-estradiol. Supplementation of TC and CJ mixture via feed admixture recovered the reduction of egg production and attenuated serological changes and gross and pathological lesions of fatty liver with the best amelioration effects at 5 and 10 g TC and CJ mixture per kg feed. In conclusion, TC and CJ mixture attenuates FLHS by means of antioxidative effects. Further mechanistic study is required to explain TC and CJ's amelioration effects against FLHS in laying hens.

• Pediatric Gastroenterology, Hepatology & Nutrition
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• 제26권2호
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• pp.99-115
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• 2023

Purpose: Exclusive breastfeeding promotes gut microbial compositions associated with lower rates of metabolic and autoimmune diseases. Its cessation is implicated in increased microbiome-metabolome discordance, suggesting a vulnerability to dietary changes. Formula supplementation is common within our low-income, ethnic-minority community. We studied exclusively breastfed (EBF) neonates' early microbiome-metabolome coupling in efforts to build foundational knowledge needed to target this inequality. Methods: Maternal surveys and stool samples from seven EBF neonates at first transitional stool (0-24 hours), discharge (30-48 hours), and at first appointment (days 3-5) were collected. Survey included demographics, feeding method, medications, medical history and tobacco and alcohol use. Stool samples were processed for 16S rRNA gene sequencing and lipid analysis by gas chromatography-mass spectrometry. Alpha and beta diversity analyses and Procrustes randomization for associations were carried out. Results: Firmicutes, Proteobacteria, Bacteroidetes and Actinobacteria were the most abundant taxa. Variation in microbiome composition was greater between individuals than within (p=0.001). Palmitic, oleic, stearic, and linoleic acids were the most abundant lipids. Variation in lipid composition was greater between individuals than within (p=0.040). Multivariate composition of the metabolome, but not microbiome, correlated with time (p=0.030). Total lipids, saturated lipids, and unsaturated lipids concentrations increased over time (p=0.012, p=0.008, p=0.023). Alpha diversity did not correlate with time (p=0.403). Microbiome composition was not associated with each samples' metabolome (p=0.450). Conclusion: Neonate gut microbiomes were unique to each neonate; respective metabolome profiles demonstrated generalizable temporal developments. The overall variability suggests potential interplay between influences including maternal breastmilk composition, amount consumed and living environment.

• 동아시아식생활학회지
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• 제18권6호
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• pp.918-926
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• 2008

본 연구는 2006년 1월부터 2007년 5월까지 수축기/이완기 혈압이 120/80 mmHg이상인 고혈압 전단계 및 본태성 고혈압 환자 43명 (대조군 21명, 시험군 23명)을 대상으로 12주간의 의학 영양 치료와 병행하여 생리활성 펩타이드가 함유된 유산균 발효유 섭취가 혈압에 미치는 영향을 조사한 결과를 요약하면 다음과 같다. 1. 본 연구 대상자의 평균 연령은 대조군이 $47.8{\pm}12.9$세, 시험군은 $49.9{\pm}11.9$세였으며, 연구 시작 시 혈압은 각 군에서 수축기/이완기 혈압이 $135.4{\pm}8.8/85.0{\pm}6.9$, $135.7{\pm}7.4/88.0{\pm}5.5\;mmHg$로 군 간의 차이가 없었다. 2. 식이 섭취 조사 결과, 연구 12주 후 대조군, 시험군 모두 열량, 단백질, 지질, 콜레스테롤, 나트륨의 섭취가 유의적으로 감소하였다(p<0.05). 칼슘과 칼륨의 섭취는 시험군에서만 유의적으로 증가하였으며, 다른 영양소의 섭취는 군 간에는 유의적인 차이를 보이지 않았다. 3. 연구 기간 동안 수축기 혈압은 대조군과 시험군 모두 연구 4주 후부터 유의적인 감소를 보였으며, 연구 12주까지 두 군 모두에서 유의적으로 감소하였다(p<0.05). 이완기 혈압은 시험군에서만 연구 4주 후부터 유의적으로 감소하였다(p<0.05). 결론적으로, 고혈압 전단계 및 고혈압 환자에게 12주간의 의학 영양 치료와 함께 유산균 발효유의 섭취는 총열량, 지방, 콜레스테롤, 나트륨의 섭취를 감소시켰고 칼슘 및 칼륨의 섭취를 증가시켰으며 수축기와 이완기 혈압을 유의적으로 감소시켰다. 따라서 생리활성 펩타이드가 함유된 유산균 발효유를 의학영양치료와 병행하여 섭취할 경우, 수축기 혈압감소뿐만 아니라 이완기 혈압 감소 모두에 긍정적인 영향을 미칠 것으로 기대한다.

• Asian-Australasian Journal of Animal Sciences
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• 제22권7호
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• pp.1005-1015
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• 2009

Two experiments were carried out to determine the utility of sainfoin hay, a temperate tanniferous forage legume, as a dietary supplement for grass-fed cows. The condensed tannins (CT) of sainfoin might counteract the typical metabolic ammonia load of cows in intensive grazing systems. Furthermore, the physical fibrousness of sainfoin might improve ruminal pH stability. In the preliminary experiment, the eating rate of non-lactating Holstein cows of two tanniferous hays, sainfoin and birdsfoot trefoil, was compared to that of a grass-clover hay after specific periods of time (n = 4). The eating rate of sainfoin was superior to that of the other forages. In the main experiment, designed as a replicated 3${\times}$3 Latin square, six ruminally-cannulated, lactating Red Holstein cows received grass, concentrate and either no supplementation, 3 kg/d of grass hay or 3 kg/d of sainfoin hay (n = 6). Measured intakes of the grass hay and the sainfoin hay were 2.0 and 1.5 kg DM, and two cows entirely refused to eat the sainfoin hay and had to be excluded from data analysis. Grass DMI was similar for cows supplemented with sainfoin hay and cows fed only grass whereas intake of concentrate was higher (p<0.01) for the latter treatment. Continuous measurement of ruminal pH showed that the minimum pH at night tended to be lower (p<0.10) with grass-only feeding compared to sainfoin supplementation, but pH did not decline below the threshold of subacute acidosis for a longer period of time. The slightly higher intake of nitrogen (N) for cows supplemented with sainfoin hay (413 g/d) compared to cows fed only grass (399 g/d) was accompanied by an increased (p<0.05) fecal N excretion and a tendency for an increased (p<0.10) urinary N excretion. Ruminal ammonia concentration, as well as plasma and milk urea, were not affected by sainfoin supplementation. In conclusion, the lack of positive effects typical for CT might be explained either by the limited CT content of this plant species (55 g/kg DM) or the relatively low proportion of sainfoin in the total diet or both. Moreover, due to the unexpected low grass quality, the general ammonia load might have been too low for CT to have an impact.

• Animal Bioscience
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• 제35권2호
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• pp.204-216
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• 2022

Objective: The aim of this study was to investigate the effects of dietary supplementation with a multi-strain probiotic (MSP) product containing of Bifidobacterium animalis, Lactobacillus casei, Streptococcus faecalis, and Bacillus cerevisiae on growth, health, and fecal bacterial composition of dairy calves during the first month of life. Methods: Forty Holstein calves (24 female and 16 male) at 2 d of age were grouped by sex and date of birth then randomly assigned to 1 of 4 treatments: milk replacer supplementation with 0 g (0MSP), 2 g (2MSP), 4 g (4MSP), and 6 g (6MSP) MSP per calf per day. Results: Supplementation of MSP did not result in any significant differences in parameters of body measurements of calves during the 30 d period. As the dosage of MSP increased, the average daily gain (p = 0.025) and total dry matter intake (p = 0.020) of calves showed a linear increase. The fecal consistency index of the 2MSP, 4MSP, and 6MSP group calves were lower than that of the 0MSP group calves (p = 0.003). As the dosage of MSP increased, the concentrations of lactate dehydrogenase (p = 0.068) and aspartate aminotransferase (p = 0.081) in serum tended to decrease, whereas the concentration of total cholesterol increased quadratically (p = 0.021). The relative abundance of Dorea in feces was lower (p = 0.011) in the 2MSP, 4MSP, and 6MSP group calves than that in the 0MSP group calves. The relative abundance of Dorea (p = 0.001), Faecalibacterium (p = 0.050), and Mitsuokella (p = 0.030) decreased linearly, whereas the relative abundance of Prevotella tended to increase linearly as the dosage of MSP increased (p = 0.058). Conclusion: The MSP product can be used to reduce the diarrhea, improve the performance, and alter the composition of the fecal bacteria in neonatal dairy calves under the commercial conditions.

• Asian-Australasian Journal of Animal Sciences
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• 제13권12호
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• pp.1699-1707
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• 2000

Thirty-one multiparous Bos indicus${\times}$Bos taurus cows were offered concentrate supplements based on (1) 2 kg wheat bran; (2) 4 kg wheat bran; and (3) concentrate (30 maize: 67 wheat bran) at 0.5 kg per 1.0 kg milk produced, in a one year study in India. All supplements also contained 2 parts of a mineral mixture and 1 part salt. Cows were allocated to treatments at calving on parity (2nd and 3rd calf) with 13, 8 and 10 cows respectively in treatments 1, 2 and 3. They were individually fed for whole lactation, the basal diet being ad libitum berseem clover plus 2 kg wheat straw in the cool season/winter (period 1) and chopped maize in summer (period 2). Diets with berseem offered TDN and CP contents of 67.6, 18.2; 65.5, 16.8; and 67.5, 16.8 percent; and with maize fodder 62.6, 12.0; 62.6, 12.5; and 63.3, 12.5 percent for treatments 1, 2 and 3, respectively. Total dry matter (DM) intakes (1) 9.9 kg, (2) 10.9 kg and (3) 11.1 kg DM/day and intake of nutrients (TDN, CP) increased with level of supplementation (p<0.01), but effects of treatment on animal performance were not significant. Cow milk yields averaged (1) 7.9 kg, (2) 8.1 kg and (3) 8.8 kg milk/day (p>0.05) for lactation lengths of 252, 270 and 220 days (p>0.05) and cows gained +7.3; +8.1; and +12.0 kg respectively over their lactation (p>0.05). Wheat bran was used effectively as the sole energy component in concentrates for lactating dairy cows. Its use could potentially reduce feed costs and demands for cereal grain. Reduced concentrate levels may be considered if green forages of high nutrient content are fed ad libitum. Associated economic advantages or disadvantages require further evaluation.

• 한국식품과학회지
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• 제32권6호
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• pp.1414-1417
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• 2000

젖산균(Streptococcus thermophilus KCTC 2185와 Lactobacillus acidophilus ATCC 4356) 발효유가 흰쥐기관의 알루미늄 축적 감소효과를 조사하기 위하여 사료에 알루미늄화합물$[Al_2(SO_4)_3]$을 혼화하여 먹이면서 음용수로 증류수를 준 대조군, 증류수 대신 S. thermophilus 발효시킨 발효유(ST)와 이를 살균한 발효유(STS), L. acidophilus로 발효시킨 발효유(LA)와 이를 살균한 발효유(LAS)를 먹게 한 군으로 나누어 4주 동안 사육한 후 뇌, 뼈, 폐, 심장, 간, 소장, 대장에 축적된 알루미늄 함량을 조사하였다. 대조군의 체 조직에 축적된 알루미늄 양은 뼈>폐>심장>대장>신장>간>뇌>소장의 순이었으며, S. thermophilus와 L. acidophilus로 발효된 발효유와 그 발효유를 멸균시킨 것을 각각(STS, ST 와 LAS, LA) 섭취한 군은 각각의 대조군(100%)에 비하여 축적량이 뼈의 경우 각각 46.71%, 51.03%와 66.57%, 79.23%, 폐의 경우 각각 19.04%, 29.20%와 67.73%, 76.98%, 심장의 경우 각각 50.72%, 56.46%와 72.59%, 86.63%, 대장의 경우 각각 29.94%, 35.94%와 53.81%, 81.01%, 간의 경우 각각 20.89%, 47.43%와 60.39%, 82.10%, 뇌의 경우 각각 21.82%, 38.33%와 63.85%, 81.15%, 소장의 경우 각각 3.97%, 14.13%와 60.43%, 62.42% 감소되는 효과가 있었다. 알루미늄화합물을 흡수한 흰쥐 체내 알루미늄 축적량 감소는 젖산균 발효유가 멸균시킨 젖산균 발효유보다 효과가 컸다.

• 대한한방소아과학회지
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• 제26권1호
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• pp.70-78
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• 2012

Objectives Recently, vitamin D supplements to breast-fed infants are being encouraged in Korea, and other countries. However, the reliability and validity assessment of supplements is insufficient. Therefore, this study suggests new Korean guidelines for vitamin D supplements of breast-fed infant. Methods The roles of Vitamin D for bone metabolism in children and the correlation between breast milk and Vitamin D were examined throughout domestic and international literature review. In addition, the efficacy and safety of vitamin D supplements were reviewed. Results Preventive effects of rickets by vitamin D supplementation remain unclear. Furthermore, concerns about the safety of vitamin D supplements intake have been raised. Korean guideline suggests breast-fed infants can get vitamin D through the skin safely, but maternal intake of vitamins through sunbath and diet is more effective and safe. Conclusions Limiting sunlight excessively and applying foreign countries' medical guideline for vitamin D supplements are not valid to apply as a domestic guideline for Korean breast-fed infants without considering ethnic and cultural characteristics.

• Clinical and Experimental Pediatrics
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• 제56권10호
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• pp.417-423
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• 2013

Vitamin D is an essential component of bone and mineral metabolism; its deficiency causes growth retardation and skeletal deformities in children and osteomalacia and osteoporosis in adults. Hypovitaminosis D (vitamin D insufficiency or deficiency) is observed not only in adults but also in infants, children, and adolescents. Previous studies suggest that sufficient serum vitamin D levels should be maintained in order to enhance normal calcification of the growth plate and bone mineralization. Moreover, emerging evidence supports an association between 25-hydroxyvitamin D (25[OH]D) levels and immune function, respiratory diseases, obesity, metabolic syndrome, insulin resistance, infection, allergy, cancers, and cardiovascular diseases in pediatric and adolescent populations. The risk factors for vitamin D insufficiency or deficiency in the pediatric population are season (winter), insufficient time spent outdoors, ethnicity (non-white), older age, more advanced stage of puberty, obesity, low milk consumption, low socioeconomic status, and female gender. It is recommended that all infants, children, and adolescents have a minimum daily intake of 400 IU ($10{\mu}g$) of vitamin D. Since the vitamin D status of the newborn is highly related to maternal vitamin D levels, optimal vitamin D levels in the mother during pregnancy should be maintained. In conclusion, given the important role of vitamin D in childhood health, more time spent in outdoor activity (for sunlight exposure) and vitamin D supplementation may be necessary for optimal health in infants, children, and adolescents.