• Asian Pacific Journal of Cancer Prevention
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• 제15권6호
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• pp.2777-2783
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• 2014

The purpose of this study was to explore the expression of ATAD2 in ovarian tumor tissue as well as its relationship with degree of malignancy. Tumor tissue from 110 cases of ovarian cancer was collected in accordance with the Declaration of Helsinki for evaluation of ATAD2 expression iimmunohistochemistry, quantitative PCR (qPCR) and Western blotting. The correlation between the ATAD2 expression and and the prognosis of ovarian cancer was evaluated by Cox regression model. In addition, HO-8910 and OVCAR-3 cells were transfected with two siRNAs targeting ATAD2. Cell viability was evaluated with MTT assay, and cell migration by transwell migration assay. ATAD2 was shown to be highly expressed in 65.5% (72/110) of ovarian cancer cases, both at transcriptional and protein levels. Moreover, highly expression was positively correlated with degree of malignancy. Knock-down of ATAD2 in HO-8910 and OVCAR-3 cells was found to reduce cell migration. In addition, follow-up visits of the patients demonstrated that the 5-year survival rate was lower in patients with high expression of ATAD2. Our study suggested that ovarian tumor tissue may have highly expressed ATAD2, which is associated with tumor stage, omentum-metastasis, ascites and CA-125. Increased ATAD2 may play important roles in tumor proliferation and migration. ATAD2 could serve in particular as a prognostic marker and a therapeutic target for ovarian cancer.

• 가족자원경영과 정책
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• 제14권2호
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• pp.153-176
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• 2010

The purpose of this study was to investigate the relationship between marriage migration females' acculturative stress and marital adjustment, especially the mediating and moderating effects of ego-resilience. The structural equation models were tested by setting marriage migration females' acculturative stress as a prediction variable, their marital adjustment as an outcome variable and their ego-resilience as mediator and moderator variables. The sample consisted of 172 marriage migration females from Busan. I analyzed the data using correlation analysis in order to discover the correlation coefficient of those variables among acculturative stress, ego-resilience and marital adjustment. I used the structural equation model (SEM) for investigating the relationship among acculturative stress, ego-resilience and marital adjustment and for investigating the mediating effects of ego-resilience. I also used multiple group analysis and two way ANOVA to investigate the moderating effects of ego-resilience. The results of structural equation modeling were as follows: first, it was proved that ego-resilience was exerted as a mediating variable, because acculturative stress appeared to affect marital adjustment in relation to ego-resilience. Therefore, marital adjustment was evident when ego-resilience was low. Second, it was proved that ego-resilience was exerted as a moderating variable, because those with low ego-resilience experienced high acculturative stress and low marital adjustment, and those with high ego-resilience experienced low acculturative stress and high marital adjustment. Such findings point out the importance of considering personal characteristics, like ego-resilience, in the relationship between acculturative stress and marital adjustment.

• 한국컴퓨터정보학회논문지
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• 제12권5호
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• pp.57-64
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• 2007

이동 에이전트는 분산 응용을 위한 효율적인 통신 패러다임으로 기존의 통신 패러다임 높은 유연성과 성능을 제공하는 것으로 알려져 왔다. 그러나 이런 장점에도 불구하고 이동 에이전트가 널리 사용되지 않는 이유는 이동 에이전트가 갖는 보안 취약성 때문이다. 이를 해결하기 위해 이동 에이전트 보안에 대한 연구가 진행되었지만 대부분의 경우 높은 보안성 제공만을 목표로 하여 성능 저하를 유발하였다. 따라서 이동 에이전트가 분산 응용 개발에 적용되기 위해서는 보안성과 성능이 모두 고려되어야 한다. 본 논문에서는 높은 보안성을 제공하는 통합 보안 관리자를 이용하여 이동 에이전트의 이주 성능을 향상시키는 방법을 제안하고 그 성능을 평가하였다. 제안한 방법에서 통합 보안 관리자는 자신이 관리하는 신뢰 도메인 내의 이주 계획을 모두 수행한 후에 작업 결과를 반환하도록 여행안내 서비스를 제공한다. 제안한 방법이 기존의 방법보다 $33{\sim}82%$ 향상된 성능을 제공함을 알 수 있다.

• Journal of Acupuncture Research
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• 제22권2호
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• pp.171-180
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• 2005

Background & Objective : Angiogenesis consists of the proliferation, migration, and differentiation of endothelial cells, and angiogenic factors and matrix protein interactions modulate this process. The aim of this study was to determine whether Puerariae radix could induce angiogenic activity in human umbilical vein endothelial cells (HUVECs). Methods: The angiogenic activity of Puerariae radix were evaluated by using BrdU assay, chemotactic migration assay, tube formation assay, measurement of bFGF in HUVECs, and Matrigel plug assay in mice. Results : Puerariae radix significantly increased HUVECs proliferation in a dose-dependent manner. In addition, Puerariae radix increased migration and tube-like formation in HUVECs. Interestingly,the expression of basic fibroblast growth factor (bFGF), an angiogenesis-stimulating growth factor, was dose-dependently increased by Puerariae radix. The angiogenic activity of Puerariae radix was confirmed using an in vivo Matrigel angiogenesis model, showing promotion of blood vessel formation. Conclusion : Puerariae radix significantly induces angiogenesis in vitro and in vivo. These results suggest that Puerariae radix is a potent angiogenic agent, and a promising drug, for the induction of neovascularization.

• 정보처리학회논문지D
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• 제11D권2호
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• pp.351-360
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• 2004

대부분의 이동 에이전트는 순차적인 노드 이주 방식에 의하여 다수의 이동 에이전트 시스템들로 이주된다. 하지만, 이러한 이주방식에서는 호스트의 결점이나 장애 등과 같은 문제가 발생하였을 경우, 이동 에이전트가 무한 대기나 고아 상태에 빠지므로 노드들 사이의 네트워크 소요시간이 증가하기 때문에 실제 다른 분산 기술들을 사용한 것만큼의 기대효과를 얻기가 어렵다. 따라서 이러한 문제를 해결하기 위한 연구들이 진행되어 왔지만 대부분 수동적 라우팅 테이블을 기반으로 노드의 이주를 결정하거나 문제가 발생된 호스트를 선회하는 방법이므로 실제 전체적인 네트워크 소요시간을 감소시키기 위한 연구는 아직 미흡하다. 본 논문에서는 이동 에이전트가 이동 에이전트 시스템들로 이주시 네트워크 소요시간을 감소시키기 위하여 네이밍 에이전트의 메타-테이블에 등록된 구현 객체 정보를 기반으로 능동적 라우팅 테이블을 설계한다. 또한 사용자의 키워드에 대하여 메타-테이블에서 일치하는 객체 참조자의 정보와 수에 따라 다수의 에이전트 객체를 복제한다. 복제된 객체는 이동에이전트 시스템들로 병렬 이주되며, 최소의 네트워크 소요시간을 제공한다.

• Ecology and Resilient Infrastructure
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• 제5권4호
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• pp.276-284
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• 2018

하단배출 형태의 연직수문을 대상으로 퇴적토 배출특성에 따른 두부침식 거리비, 퇴적토 이동거리와 이동량을 분석하기 위해 수리 모형실험과 차원해석을 수행하였다. Froude 수와 배출특성의 상관관계를 도식화하고, 퇴적토 배출특성을 지배하는 무차원 매개변수에 의한 다중회귀식을 제안하였다. 두부침식거리, 퇴적토 이동거리와 이동량에 대한 각 다중회귀 분석식의 결정계수는 각각 0.618, 0.632, 0.866으로 높게 나타났다. 개발한 퇴적토 배출특성식의 사용성을 평가하기 위해 실제 측정값과 회귀분석식에 의해 계산된 값의 95%의 예측 신뢰구간 분석을 수행하였고, 두부침식거리, 퇴적토 이동거리와 이동량에 대한 예측의 정확도 분석차원의 NSE (Nash-Sutcliffe Efficiency), RMSE (root mean square)와 MAPE (mean absolute percentage error)는 적절한 것으로 판단되었다.

• 지역연구
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• 제35권2호
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• pp.49-57
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• 2019

생애주기단계에서 청년층은 그 사회적위치가 빠르게 변화하는 연령대로, 본 연구는 청년층의 인구이동에 주목하고 주택시장요인을 포함한 지역특성요인과 청년층 인구이동의 관계를 서울지역을 중심으로 실증 분석하였다. 분석결과, 청년층의 이동은 거리와 일자리는 중요한 이동의 원인이 되고 있다. 이외에 주택시장에서의 전세가격이나 주택공급이 청년층 이동에 유의한 영향 요인으로 작용하는 것으로 분석되었다. 또한 서울시내 개별 지역과 이동의 기원지간의 전세가격과 같은 가격의 차이가 커질수록, 주택공급 차이가 커질수록 서울시 청년층의 순전출이 지속될 수 있음을 유추할 수 있다. 분석 결과 일자리의 경우, 청년층 유입을 증가시키는 데 기여하고 있어 고용중심지 주변에서의 청년층 주거수요는 높아질 것으로 예상된다. 따라서 현재 서울시나 정부에서 추진하고 있는 일자리가 많은 지역주변이나 역세권 주변에서의 청년층을 대상으로 한 공공주택을 비롯한 민간의 주택공급이 원활하게 이루어질 필요가 있으며, 저렴한 가격으로 거주할 수 있는 공적 주택이나 민간임대 주택 공급이 지속된다면, 서울시 청년층 전출을 줄이는 데 기여할 수 있을 것으로 기대된다.

• Journal of Radiation Protection and Research
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• 제46권2호
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• pp.58-65
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• 2021

Background: The evaluation of skyshine distribution, release of airborne radioactive nuclides, and soil activation and groundwater migration were required for radiological assessment of the impact on the environment surrounding In-Flight (IF)-system facility of the RAON (Rare isotope Accelerator complex for ON-line experiment) accelerator complex. Materials and Methods: Monte Carlo simulation by MCNPX code was used for evaluation of skyshine and activation analysis for air and soil. The concentration model was applied in the estimation of the groundwater migration of radionuclides in soil. Results and Discussion: The skyshine dose rates at 1 km from the facility were evaluated as 1.62 × 10^-3 μSv·hr^-1. The annual releases of ³H and ¹⁴C were calculated as 9.62 × 10^-5 mg and 1.19 × 10^-1 mg, respectively. The concentrations of ³H and ²²Na in drinking water were estimated as 1.22 × 10^-1 Bq·cm^-3 and 8.25 × 10^-3 Bq·cm^-3, respectively. Conclusion: Radiological assessment of environmental impact on the IF-facility of RAON was performed through evaluation of skyshine dose distribution, evaluation of annual emission of long-lived radionuclides in the air and estimation of soil activation and groundwater migration of radionuclides. As a result, much lower exposure than the limit value for the public, 1 mSv·yr^-1, is expected during operation of the IF-facility.

• The Korean Journal of Physiology and Pharmacology
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• 제26권5호
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• pp.347-355
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• 2022

Abdominal aortic aneurysm (AAA) is a life-threatening disorder worldwide. Fibroblast growth factor 21 (FGF21) was shown to display a high level in the plasma of patients with AAA; however, its detailed functions underlying AAA pathogenesis are unclear. An in vitro AAA model was established in human aortic vascular smooth muscle cells (HASMCs) by angiotensin II (Ang-II) stimulation. Cell counting kit-8, wound healing, and Transwell assays were utilized for measuring cell proliferation and migration. RT-qPCR was used for detecting mRNA expression of FGF21 and activating transcription factor 4 (ATF4). Western blotting was utilized for assessing protein levels of FGF21, ATF4, and markers for the contractile phenotype of HASMCs. ChIP and luciferase reporter assays were implemented for identifying the binding relation between AFT4 and FGF21 promoters. FGF21 and ATF4 were both upregulated in Ang-II-treated HASMCs. Knocking down FGF21 attenuated Ang-II-induced proliferation, migration, and phenotype switch of HASMCs. ATF4 activated FGF21 transcription by binding to its promoter. FGF21 overexpression reversed AFT4 silencing-mediated inhibition of cell proliferation, migration, and phenotype switch. ATF4 transcriptionally upregulates FGF21 to promote the proliferation, migration, and phenotype switch of Ang-II-treated HASMCs.

• Nutrition Research and Practice
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• 제4권5호
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• pp.362-368
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• 2010

Oral administration of royal jelly (RJ) promotes wound healing in diabetic mice. Concerns have arisen regarding the efficacy of RJ on the wound healing process of normal skin cells. In this study, a wound was created by scratching normal human dermal fibroblasts, one of the major cells involved in the wound healing process. The area was promptly treated with RJ at varying concentrations of 0.1, 1.0, or 5 mg/ml for up to 48 hrs and migration was analyzed by evaluating closure of the wound margins. Furthermore, altered levels of lipids, which were recently reported to participate in the wound healing process, were analyzed by HPTLC and HPLC. Migration of fibroblasts peaked at 24 hrs after wounding. RJ treatment significantly accelerated the migration of fibroblasts in a dose-dependent manner at 8 hrs. Although RJ also accelerated the migration of fibroblasts at both 20 hrs and 24 hrs after wounding, the efficacy was less potent than at 8 hrs. Among various lipid classes within fibroblasts, the level of cholesterol was significantly decreased at 8 hrs following administration of both 0.1 ug/ml and 5 mg/ml RJ. Despite a dose-dependent increase in sphinganines, the levels of sphingosines, ceramides, and glucosylceramides were not altered with any concentration of RJ. We demonstrated that RJ enhances the migration of fibroblasts and alters the levels of various lipids involved in the wound healing process.