• Title/Summary/Keyword: midgut

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cDNA Sequence and mRNA Expression of a Novel Serine Protease from the Firefly, Pyrocoelia rufa

  • Lee, Kwang-Sik;Kim, Seong-Ryul;Sohn, Hung-Dae;Jin, Byung-Rae
    • International Journal of Industrial Entomology and Biomaterials
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    • v.5 no.1
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    • pp.103-108
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    • 2002
  • We describe here the cDNA sequence and mRNA expression of a novel serine pretense from the firefly, Pyrocoelia rufa. The 771 bp cDNA encodes for 257 amino acid residues. The deduced protein of P. rufa serine pretense gene contains the catalytic triad and six-conserved cysteine residues. Alignment of the deduced protein of P. rufa serine pretense gene showed 47.4% protein sequence identity to known coleopteran insect Rhyzopertha dominica midgut trpsin-like enzyme. Northern blot analysis revealed that the P. rufa serine pretense is specifically expressed in the midgut of P. rufa larvae.

A Case of Midgut Volvulus with Gastric Perforation and Periveintricular Leukomalacia in a Term Infant

  • Park, Seul Gi;Hwang, Jong Hee
    • Neonatal Medicine
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    • v.28 no.1
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    • pp.53-58
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    • 2021
  • Intestinal malrotation with midgut volvulus (MV) is a life-threatening surgical emergency. Most events of MV occur in the neonatal period with bilious vomiting, abdominal distension, feeding intolerance, and bloody stools. Neonatal gastric perforation (GP) is a rare and life-threatening condition associated with high mortality. It occurs either in an idiopathic form or in association with gastrointestinal anomalies such as duodenal atresia and MV. The pathogenesis of both MV and GP is related to ischemic change and inflammatory response. MV and GP can lead to morbidities such as sepsis, intestinal ischemia, and organ failure, but not neurologic problems. We herein report the case of a term infant at 5 days after birth, with MV accompanied by GP, who developed periventricular leukomalacia.

Gastrointestinal Emergency in Neonates and Infants: A Pictorial Essay

  • Gayoung Choi;Bo-Kyung Je;Yu Jin Kim
    • Korean Journal of Radiology
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    • v.23 no.1
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    • pp.124-138
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    • 2022
  • Gastrointestinal (GI) emergencies in neonates and infants encompass from the beginning to the end of the GI tract. Both congenital and acquired conditions can cause various GI emergencies in neonates and infants. Given the overlapping or nonspecific clinical findings of many different neonatal and infantile GI emergencies and the unique characteristics of this age group, appropriate imaging is key to accurate and timely diagnosis while avoiding unnecessary radiation hazard and medical costs. In this paper, we discuss the radiological findings of essential neonatal and infantile GI emergencies, including esophageal atresia and tracheoesophageal fistula, hypertrophic pyloric stenosis, duodenal atresia, malrotation, midgut volvulus for upper GI emergencies, and jejunoileal atresia, meconium ileus, meconium plug syndrome, meconium peritonitis, Hirschsprung disease, anorectal malformation, necrotizing enterocolitis, and intussusception for lower GI emergencies.

Secretory Granul Cells in the Midgut Epithelium of the Blattella germanica L. (바퀴의 중장 상피조직내에 있는 분비과립세포)

  • Yu, Chai-Hyeock;Kim, Woo-Kap;Kim, Chang-Whan
    • Applied Microscopy
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    • v.10 no.1_2
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    • pp.1-6
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    • 1980
  • The secretory granul cells in the midgut epithelium of Blattella germanica L. were observed by the electron microscope. These secretory granul cells contain many electron dense granules, and granules are about $200{\AA}$ in diameter respectively. It is easy to distinguish 3 different types of granul cells based on their shapes, location, and staining intensity: 1) The light secretory granul cells and their nucleus are both round form and a number of mitochondria, vacuoles, and other cell organelles appear in the cytoplasm. 2) The other kind of light secretory granul cells are small and oval form but ceil organelles are not well developed in the cytoplasm. This granul cell is surrounded by a few regenerative cells ('nidi'). 3) Dark secretory granul cells are cone shaped, well stained, and endoplasmic reticulum, ribosomes, and a lot of secretory granules are found in the cytoplasm. They are all located in the basal portion of the midgut epithelium.

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Age-related Differences Effect the Clinical Characteristics of Intestinal Malrotation (장 회전이상 환자에서 연령에 따른 임상적 특성의 차이)

  • Kim, Jong-Jin;Jung, Kyu-Whan;Park, Tae-Jin;Jung, Sung-Eun;Park, Kwi-Won
    • Advances in pediatric surgery
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    • v.15 no.2
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    • pp.121-131
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    • 2009
  • Intestinal malrotation presents with different clinical characteristics, depending upon the age of the patient. The medical records of 44 patients treated for intestinal malrotation with/without midgut volvuls between January 2002 and August 2009 at Seoul National University Children's Hospital were reviewed retrospectively. Patients were grouped by the age criteria of 1 and 12 months. Fourteen patients were under 1 month of age (31.8 %), 9 patients between 1 month and 12 months (20.5 %) and 21 patients over 12 months (47.7 %). Twenty patients (45.5 %) presented with volvulus. Vomiting (66 %) and abdominal pain (20 %) were the most common symptoms. UGIS (68.2 %) was the most frequent diagnostic tool. Mean postoperative hospital stay was 13.7 days. There were 7 mild postoperative complications. Volvulus was more frequent in patients under 1 month (p=0.025) than over 1 month. The interval between diagnosis and operation was shorter in patients under 1 month (p=0.003) than in patients over one month of age. In the age between under and over 12 months, volvulus was more common in those under 12 months of age, but the difference was not significant. The interval from diagnosis to operation was shorter in patients under 12 months than over 12 months of age (p=0.001). Vomiting was the most frequent symptom in patients under 12 months. On the other hand, abdominal pain was the most frequent symptom in patients over 12 months. In conclusion, patients with intestinal malrotation had age-related differences in the presence of midgut volvulus, the interval between diagnosis and operation, and clinical symptoms. The age of the patient should be considered in order to determine adequate treatment of malrotation.

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Activity of Alkaline Phosphatase from the Mosquito, Culex pipiens pallens (홍모기(Culex pipiens pallens)의 난성숙 과정 중 alkaline phosphase의 활성)

  • 이영수;이승훈;박영민;성기창
    • The Korean Journal of Zoology
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    • v.36 no.3
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    • pp.425-432
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    • 1993
  • Alkaline phosphatase from Culex pipiens pallens was examined to determine the optimal assay condition and to assay the activity during ovarian development. The activity of alkaline phosphatase in a male and a nongravid female continuously were declined after eclosion. But by the stimulus of a blood meal, the enzyme activity was increased dramatically. At 30 hr. after a blood meal, the maximal activity was reached and then declined. And after 48 hr. after a blood meal, the second activity increase was revealed. This second increase was maintained up to oviposition. The first activity increase was revealed in the midgut and the second increase was done in the ovary to assay the organ distribution of alkaline phosphatase. In electrophresis data, it was shown 5 isozyme bands, ALP-1 and ALP-2 in the ovary, ALP-3 in the thorax and the midgut, and ALP-4 and ALP-5 in the thorax, the fatbody and the midgut in crude extract at 30 hr. after a blood meal. One the same ovary pattern were shown at 72 hr. after a blood meal.

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