• 한국광물학회지
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• 제4권1호
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• pp.51-55
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• 1991

산청 할로이사이트의 전자 회절분석 및 레플리카와 박편을 이용한 형태 연구결과 관산 할로이사이트는 반경이 서로 다른 몇 개의 관들이 중첩되어 있으며 내부의 작은 관은 원형이나 외부의 큰 관은 다각형으로서 두 관 사이에 삼각형의 공극이 형성되어 있음이 관찰되었다. 할로이사이트의 단면은 대체로 직경이 500${\AA}$보다 작으면 원형을 이루고 그보다 크면 다각형을 이룬다. 다각형의 단면을 갖는 할로이사이트의 전자 회절상은 이들이 이층 단사 구조를 갖고 있음을 지시한다.

• 대한한의학회지
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• 제35권4호
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• pp.83-97
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• 2014

Objectives: This study aimed for establishing the discriminative criteria for Aucklandiae Radix, Inulae Radix, Vladimiriae Radix and Aristolochiae Radix, which can be confused as 'Mokhyang', because of similar appearances and synonyms. Methods: Morphological characteristics of dried herbal medicines were compared by macroscopic observation. To examine microscopic features of 'Mokhyang', paraffin embedding and the staining by using Ju's method were conducted. Physicochemical experiments were performed using HPLC analysis and antioxidant assay. Results: The types of stem, phyllotaxy and leaf shape were chosen as macroscopic criteria and the size, oil spots, color and root top as well as phloem and central cylinder were compared using microscopic features. The HPLC results showed different amounts of costunolide and dehydrocostuslactone among Aucklandiae Radix, Inulae Radix and Vladimiriae Radix. However, Aristolochiae Radix only contained aristolochic acid. The antioxidant assays also showed that Vladimiriae Radix exhibited strongest antioxidant activity followed by Aucklandiae Radix, Inulae Radix and Aristolochiae Radix. Conclusions: These results demonstrate that macroscopic, microscopic and physicochemical methods would be useful tools for the discrimination of 4 kinds of 'Mokhyang'.

• Applied Microscopy
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• 제42권2호
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• pp.111-114
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• 2012

The scanning electron microscopy is an ideal technique for examining plant surface at high resolution. Most hydrate samples, however, must be fix and dehydrate for observation in the scanning electron microscope. Because the microscopes operate under high vacuum, most specimens, especially biological samples, cannot withstand water removal by the vacuum system without morphological distortion. Cryo-techniques can observe in their original morphology and structure without various artifacts from conventional sample preparation. Rapid cooling is the method of choice for preparing plant samples for scanning electron microscopy in a defined physiological state. As one of cryo-technique, high-pressure freezing allows for fixation of native non-pretreated samples up to $200{\mu}M$ thick and 2 mm wide with minimal or no ice crystal damage for the freezing procedure. In this study, we could design to optimize structural preservation and imaging by comparing cryo-SEM and convention SEM preparation, and observe a fine, well preserved Arabidopsis stem's inner ultrastructure using HPF and cryo-SEM. These results would suggest a useful method of cryo-preparation and cryo-SEM for plant tissues, especially intratubule and vacuole rich structure.

• 대한수의학회지
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• 제39권6호
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• pp.1057-1065
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• 1999

The study was designated to investigate the electron microscopic findings following diethylnitrosamine (DEN) treatment in rats. Forty four male (Srague Dawley) rats were continuously given water containing 0.01% DEN for 13 weeks and livers of five rats with more tumor lesions at 16 and 17 weeks after initial treatment were used as EM materials. In transmission electron microscopic findings, most small-sized hepatocytes were active cells containing large mount of organelles, but light (pale staining) hepatocytes among small-sized hepatocytes were injured cells containg disorganized organelles. Tumor cells among small-sized hepatocytes were irregularly arranged and have pleomorphic nuclei containing electron dense chromatin but the organelles in cytoplasm were swelled. Large-sized hepatocytes were active cells with condensed chromatin but the cytoplasm of these cells were pale due to be injured and dilated organelles. Dark hepatocytes were apoptotic cells with homogenous pyknotic nuclei and cytoplasm, and the cytoplasm of these cells contained dilated smooth endoplasmic reticulum (sER) but these sER were non-vesiculated. Cholangiocarninoma cells were crowded and were pale by far less number of organelles in cytoplasm and nuclei. In scanning electron microscopic findings, the lumens of portal veins, bile canaliculi, bile ductules, bile ducts and sinusoids were dilated and have irregular folded inner surface by protruded parenchyma.

• 한국세라믹학회지
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• 제12권2호
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• pp.21-25
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• 1975

As the major alumina source, domestic alunite was applied to synthesize the clinker for rapid hardening cement. The main minerals of the clinker were found to be C3S, C11A7-CaF2 and some C4AF by means of chemical treatment, x-ray diffraction analysis and microscopic observation. Rapid hardening cement was made of the clinker by adding suitable amount of hemi-hydrite and anhydrite. The setting time of the cement was regulated with citric acid as setter.

• 한국잠사곤충학회지
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• 제29권2호
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• pp.82-83
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• 1987

The number of chromosomes of the wild mulberry silkworm, Bombyx mandarina, collected in Suwon area in the middle part of Korean peninsula was investigated. The chromosomes were examined after smearing and squashing the testis of the last instar larvae which were at spining stage and the microscopic observation showed that the number of chromosomes of the wild mulberry silkworm was 27.

• Journal of Pharmaceutical Investigation
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• 제15권1호
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• pp.8-14
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• 1985

Data obtained from X-ray diffractometry, thermal analysis, IR spectroscopy and microscopic observation were used for the identification and characterization of four crystalline modifications of piroxicam. form a was crystallized from sodium hydroxide-hydrochloric acid and from c was obtained by crystallization from toluene. Form b and d was crystallized from methanol under the different temperature conditions. Relative rates of dissolution and solubility of four crystal forms of piroxicam in distilled water were measured.

• 한국식품영양학회지
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• 제2권2호
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• pp.21-26
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• 1989

The morphological changes of fresh beef treated with ficin(0.1% : 2 hrs, 6 hrs)were examined with transmission electron microscope(TEM), the results obtained were as follows ; Connective tissue protein in fresh beef treated with ficin became gradually fragmentation and was occurred solubilization with time The length of sarcomere in myofibrillar protein was elongated, M-line became dim, and the 1-band of Z-line was broken and beck me fragmentation with time.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2005년도 국제학술회의
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• pp.204-205
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• 2005

• 한국식품영양학회지
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• 제1권2호
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• pp.9-17
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• 1988

The morphological changes of fresh beef treated with ficin(0.1% : 2hrs, 6hrs) were examined with optical microscope (OM, LM), the results obtained were as follows : Connective tissue protein in fresh beef treated with ficin was occurred swelling and separation of endomysial reticulum with time, followed showing granulation and solubilization slightly. Myofibrillar protein was loosed wavy contractile muscle fiber, and showed erosin, cracks and breaks in fibers with time.