• 제목/요약/키워드: microscopic observation

검색결과 708건 처리시간 0.032초

상피 및 비상피세포들의 Intermediate Filament에 대한 면역조직화학적 및 전자현미경적 연구 (Immunohistochemical and Electron Microscopic Studies on Intermediate Filament of Epithelial Cell and Non-Epithelial Cells)

  • 김일;김효성;노영복
    • Applied Microscopy
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    • 제21권1호
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    • pp.46-62
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    • 1991
  • The intermediate filament is one of the most important constituents of the intracytoplasmic cytoskeleton microtubule, actin, myosin and intermediate filament. It is composed of keratin, desmin, vimentin, neurofilament and glial filament, and has important role as a cellular marker, epithelial or mesenchymal origin. So it will be important to differentiated from some poorly or undifferentiated neoplasm to provide adequate therapeutic modalities. This study was performed by using immunohistochemical staining and electron microscopic observation to find out intermediate filaments of epithelial and non-epithelial tumor cells evaluate the degree of differentiation in tumors and therefore to provide some diagnostic and therapeutic modalities. The materials consisted of 83 epithelial and non-epithelial elements bearing 23 normal control, 28 epithelial tumors, and 32 non-epithelial tumors, that are resected for definite treatment at Chosun University Hospital from June, 1988 to June, 1990. Immunohistochemical stain for keratin, desmin and vimentin, and electron microscopic study were performed in all cases. The results obtained were as follows. 1. Immunohistochemical stain for intermediate filament were very useful diagnostic aid for differentiated epithelial tumor to non-epithelial tumor in diagnostic neoplasia. 2. In the electron microscopic finding, the size of intermediate filaments were possible differentiated to cell components of epithelial tumor and non-epithelial tumors.

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법랑질 평골면 갈색반의 형태학적 관찰

  • 민성기
    • 대한치과의사협회지
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    • 제12권10호
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    • pp.763-766
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    • 1974
  • The electron microscopic observation on the browny discolored enamel spot on smooth surface of permanent teeth was performed by mean of the Filmy Replica method and obtained the following: 1) The browny discolored enamel spot on smooth surface of permanenet teeth was identified as a dental caries.

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차세대염기서열 및 현미경 분석을 통한 저어새의 토사물 내 먹이생물 분석 (Analyzing Vomit of Platalea minor (Black-faced Spoonbill) to Identify Food Components using Next-Generation Sequencing and Microscopy)

  • 김현정;이택견;정승원;권인기;유재원
    • 환경생물
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    • 제36권2호
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    • pp.165-173
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    • 2018
  • 저어새의 먹이생물을 파악하기 위해 2010년 6월부터 2014년 6월까지 인천 남동유수지에서 저어새의 토사물 시료를 채집하여 현미경 관찰 및 차세대염기서열(NGS) 기법으로 분석하였다. 저어새의 먹이생물은 어류, 갑각류, 다모류, 곤충류로 구성되어 있었으며, 주로 저어새는 어류와 갑각류를 섭이하는 것으로 나타났다. 최우점 먹이생물은 풀망둑(Acanthogobius hasta)이었으며, 이 외에도 길게(Macrophthalmus abbreviates), 징거미새우류(Macrobrachium sp.), 칠게(Macrophthalmus japonicus), 각시흰새우(Exopalaemon modestus), 참갯지렁이(Neanthes japonica)가 우점 먹이생물로 출현하였다. 이들 먹이생물은 번식지 인근지역인 송도갯벌과 시화호에서 흔히 발견되며, 저어새는 채식지로써 이들 지역에 대한 의존도가 높을 것으로 판단된다. 현미경과 NGS로 분석한 일부 먹이생물에서 차이를 보였는데, 이는 토사물 내 먹이생물은 저어새의 위 내에서 분해되어 현미경 분석을 통한 형태학적 분류 특징을 찾기 어려웠던 반면, NGS 분석은 유전자를 통해 분류가 가능하기 때문에 형태학적 분석의 결과보다 높은 종 다양성을 보인 결과이다.

백서난관상피(白鼠卵管上皮)의 주사현미경적(走査電子顯微鏡的) 관찰(觀察) (Scanning Electron Microscopic Observation on the Rat Oviductal Epithelium in each Segment during Normal Sexual Cycle)

  • 이재현
    • Applied Microscopy
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    • 제10권1_2호
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    • pp.41-51
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    • 1980
  • 정상(正常) 성주기(性週期)를 나타내는 백서(白鼠)의 난관(卵管)에서 각(各) 영역(領域)에 분포(分布)하는 상피세포(上皮細胞)를 주사전자현미경(走査電子顯微鏡)으로 관찰(觀察)하여 다음과 같은 결과(結果)를 얻었다. 즉, 백서난관상피세포(白鼠卵管上皮細胞)는 섬모세포와 무(無)섬모세포로 구성되며, 무(無)섬모세포는 4가지 세포형(細胞型)으로 구별(區別)할 수 있고 또한 이들 각형(各型)의 세포(細胞)는 성주기시(性週期時) 특정영역(特定領域)에 분포(分布)하였다.

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토끼의 바이러스성(性) 출혈증(出血症) 감염시(感染時) 신장(腎臟)의 광학(光學) 및 전자현미경적(電子顯微鏡的) 관찰(觀察) (Light and electron microscopy of the kidney tissues on viral haemorrhagic disease in rabbits)

  • 김진호;정치영;박남용
    • 대한수의학회지
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    • 제29권2호
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    • pp.99-108
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    • 1989
  • In order to observe the pathological changes of kidney in rabbits infected with the viral haemorrhagic disease, the kidney tissues from the 91 rabbits infected with the viral haemorrhagic disease were examined by light and electron microscopy. The results observed were as follows: 1. On light microscopic observation, the kidney lesions were identified as haemorrhagic glomerular necrosis(33.0%), membranous glomerulonephritis(20.9%), thrombotic glomerulopathy(19.8%), membranoproliferative glomerulonephritis(8.8%), mesangial proliferative glomerulonephritis(8.8%) ischemic acute tubular necrosis(7.7%), and acute serous glomerulitis(6.6%). 2. On electron microscopic observation, cytoplasmic degeneration of mesangial cells, and irregular thickening of basement membranes with electron dense granular materials were observed. In podocytes swelling of mitochondria, dilatation of endoplasmic reticulum and extensive fusion of foot processes were also observed. Nonenveloped round icosahedral picornaviral particles with a diameter of 28~33nm were detected in the cytoplasm of degenerative endothelial cells, polymorphonuclear leucoytes, and monocytes.

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직물 복합재료의 드레이핑 미소 거동 관찰 : 일방향 편향 인장실험과 이축 인장실험 (MICROSCOPIC OBSERVATION OF DRAPED COMPOSITE MATERIALS : Bias Extension and Biaxial Tests)

  • 장승환
    • Composites Research
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    • 제17권1호
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    • pp.38-46
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    • 2004
  • 본 논문의 목적은 탄소섬유 건직물의 토우 구조의 미소 거동과 변형 중의 평면 하중과의 관계를 규명하는 것이다. 평면 하중은 변형 중에 토우들을 재배치하며, 결국에는 잠김 현상을 유발하게 된다. 본 논문에서는 토우 간격, 주름각등의 미소 변형 변수들을 관찰하기 위해 일방향 편향 인장실험과 이축 인장실험을 겪은 시편들을 전단각에 따라 분류하여 현미경을 이용하여 변형 경향을 관찰하였다. 전단각에 따른 변형된 토우 구조의 변화를 간단한 설계 변수 모델을 이용하여 근사하였다.

Improvement of Predictive and Corrective Inspection Methods to Control Nosema bombycis Infection in the Silkworm, Bombyx mori

  • Nguyen, Mau-Tuan;Jon
    • 한국잠사곤충학회지
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    • 제39권2호
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    • pp.146-153
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    • 1997
  • The microsporidian infection with Nosema bombycis, reconfirmed its high virulence and transovarial tranmissibility, however, the characteristic symptom of the spots like pepper grains on the diseased larval skin was no more recognized by present varieties of the silkworm. Transovarial transmission rate detected from moth was above 90% in dead eggs or dead larvae in the rearing by mulberry leaves, 80% in the newly hatched larvae starved to death. Transovarially transmitted N. bombycis was easily observed from dead eggs and larvae, and were suggested an individual inspection of a few of dead eggs for detection of the pathogenic spores. The progeny population provided indicative factors on the sampling of predictive and corrective inspection. The higher concentration of N. bombycis spores included in the hindabdormal part of infected moth, applicative on the simple method of indivisual moth inspection. For the predictive inspection of growned 5th-instar larvae, N. bombycis infection was detectable without microscopic observation by the unique symptom of turbid milky-white spots on the silk gland. Inspection of the meconia artificially discharged from silkworm moth, was also succesful of microscopic observation before crossing, without killing or homogenize the moths. The results provided a basis of rational methods for the inspection of N. bombycis infection of the silkworm.

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